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OfflineLa Flama Blanca
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Pastywhyte]
    #23552823 - 08/18/16 10:13 AM (7 years, 5 months ago)

i don't understand why all the unyielding skepticism. there are literally parasitic mushrooms spores that are able to invade and replace host tissue so efficiently that it will wipe out an entire ant colony. doesn't seem impossible that cubes could be selectively bred to be stronger vs certain contams. just because it seems impossible doesn't mean its not worth even thinking about. how many times have human beings accomplished the seemingly impossible?


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InvisiblePastywhyteMDiscord
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: La Flama Blanca]
    #23552828 - 08/18/16 10:16 AM (7 years, 5 months ago)

Can you selectively breed a sheep to kill lions? How long would that take? Is there a possibility of such a project having unforseen negative consequences?

No one has said impossible. Improbable, expensive, time consuming, perhaps dangerous. But not impossible.

Tell ya what. You get started now, maybe aquire a few PhD's and 100 million in funds, and in 30 years when you achieve it, assuming I'm still alive, I will grow them cubes.


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OfflinePsilosopherr
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Pastywhyte]
    #23552834 - 08/18/16 10:18 AM (7 years, 5 months ago)

this information-less lion analogy does nothing for me


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InvisiblePastywhyteMDiscord
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Psilosopherr]
    #23552842 - 08/18/16 10:22 AM (7 years, 5 months ago)

Quote:

rbalzer said:
this information-less lion analogy does nothing for me




Why? It's literally the same thing. Don't like lions? Okay how about breeding hamsters that can take out elephants.

There is a reason contams beat the cubes and not the other way around. Are you not familiar with concepts like food chain? Many molds eat and parasitize cubes. Cubes are not designed to eat molds.

What information do you want? There are pics of trich hyphae using anastomosis to kill cube hyphae. They are on the site. Educate yourself.


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Offlinelukehighwalker710
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Pastywhyte]
    #23552854 - 08/18/16 10:27 AM (7 years, 5 months ago)

Quote:

Pastywhyte said:
No one has said impossible. Improbable, expensive, time consuming, perhaps dangerous. But not impossible.

Tell ya what. You get started now, maybe aquire a few PhD's and 100 million in funds, and in 30 years when you achieve it, assuming I'm still alive, I will grow them cubes.




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InvisiblePastywhyteMDiscord
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: lukehighwalker710]
    #23552875 - 08/18/16 10:36 AM (7 years, 5 months ago)

So let's toss out the mushrooms and mammal analogies cause ya ain't getting it. Let's look at plants which admittedly I know very little about.

So how about we breed or vaccinate weed plants so they produce a toxin to kill any spider mites that might infest the grow. The cannabis industry should be all over that shit. It can't be impossible right?


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InvisiblebodhisattaMDiscordReddit
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Psilosopherr] * 1
    #23552876 - 08/18/16 10:36 AM (7 years, 5 months ago)

Quote:

rbalzer said:
my one question never got answered. Does a lab strain born and bred in sterility have the same exact reaction/resistance to mold as a wild strain?



Depends on its genetics.

Brewers yeast has been in sterile conditions for its whole life it doesn't loose its abilities


Vaccination doesn't work if you don't have T-cells and a CNS


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InvisiblePastywhyteMDiscord
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: bodhisatta]
    #23552882 - 08/18/16 10:38 AM (7 years, 5 months ago)

And yet most of the genetic mods done to yeast to allow them to produce morphine etc, seem to get bred out after several generations. They are now trying to modify yeasts to produce meds like insulin but it's proving expensive and difficult.


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InvisiblebodhisattaMDiscordReddit
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Pastywhyte] * 1
    #23552939 - 08/18/16 10:58 AM (7 years, 5 months ago)

It's hard to get yeast to be a good organism for a bio reactor. IE to use yeast as a factory for some other compound it doesn't naturally make.

Whoever tried making yeast produce insulin didn't really think that out. That's why they use GMO aspergillus to make other compounds in bio reactors.

Yeast chromosomes are polyploid and have aneuploidy
So most chromosomes have more than two copies some have a bunch of copies.

So it's incredibly difficult to mutate yeast since it has a natural ability to resist genetic modification whether by human modification, replication errors, or say a cosmic ray. The bad or damaged copy gets "fixed" right Away


That's why we can run yeast 100 generations in a row and see not a single mutation appear. Actually the first mutation that almost always happens in yeast is petite mutants. Because mitochondrial DNA is more easily damaged. If that happens the yeast  respire poorly and become very small. So if you see say 5% petites its time to go back to your slants/masters


Aspergillus isn't polyploid/aneuploid so its a way better Candidate for GMO use


Edited by Trusted cuItivator (08/18/16 11:00 AM)


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InvisiblePastywhyteMDiscord
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: bodhisatta]
    #23552982 - 08/18/16 11:09 AM (7 years, 5 months ago)

Great info which further proves the point that you cannot just randomly modify species to do anything. At least not easily. Not to the point where anyone growing an illegal organisim is going to pull it off.


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OfflinePsilosopherr
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Pastywhyte]
    #23553866 - 08/18/16 04:18 PM (7 years, 5 months ago)

Quote:

Pastywhyte said:
Quote:

rbalzer said:
this information-less lion analogy does nothing for me




Why? It's literally the same thing. Don't like lions? Okay how about breeding hamsters that can take out elephants.

There is a reason contams beat the cubes and not the other way around. Are you not familiar with concepts like food chain? Many molds eat and parasitize cubes. Cubes are not designed to eat molds.

What information do you want? There are pics of trich hyphae using anastomosis to kill cube hyphae. They are on the site. Educate yourself.



I've been trying to remain respectful this whole thread, yet you continue to be an asshole :shrug:

maybe just stop replying before we say things we'll regret.


I said in the OP that this is probably another waacky impractical idea. Not that half the people here even read that before running their mouths about nothing..


Edited by Psilosopherr (08/18/16 04:22 PM)


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Offlinemindbentempire
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Psilosopherr] * 1
    #23553930 - 08/18/16 04:34 PM (7 years, 5 months ago)

Quote:

rbalzer said:
Quote:

Pastywhyte said:
Quote:

rbalzer said:
this information-less lion analogy does nothing for me




Why? It's literally the same thing. Don't like lions? Okay how about breeding hamsters that can take out elephants.

There is a reason contams beat the cubes and not the other way around. Are you not familiar with concepts like food chain? Many molds eat and parasitize cubes. Cubes are not designed to eat molds.

What information do you want? There are pics of trich hyphae using anastomosis to kill cube hyphae. They are on the site. Educate yourself.



I've been trying to remain respectful this whole thread, yet you continue to be an asshole :shrug:

maybe just stop replying before we say things we'll regret.


I said in the OP that this is probably another waacky impractical idea. Not that half the people here even read that before running their mouths about nothing..





Youve got issues man..
Pasty and TC have both been very patient, respectful and educational throughout this whole thread.
Chill out. :bongload:


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OfflinePsilosopherr
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Psilosopherr]
    #23553933 - 08/18/16 04:34 PM (7 years, 5 months ago)

Gonna post a couple quotes from the OP, combined they make all these wolf/rabbit analogies look irrelevant, because nobodies talking about that except the naysayers; I was asking if maybe the rabbit might merely get a better chance ffs. Can you breed faster rabbits at home? abso-fucking-lutely.

Quotes from OP

"The only potential benefit I see it having is more resistance to contams in the grow room. "

"Probly just another of my wacky yet useless ideas, but hopefully you at least found it interesting."


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OfflinePsilosopherr
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: mindbentempire]
    #23553943 - 08/18/16 04:39 PM (7 years, 5 months ago)

Never wanted to swing back, really pasty's first comment nearly got a rise out of me. We have very opposing philosophies apparently.

Got respect for the guy outside of this thread. Hope we can still be cool once the thread dies


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InvisibleInocuole
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Psilosopherr] * 2
    #23554002 - 08/18/16 04:52 PM (7 years, 5 months ago)

Is it really THAT hard to just accept that the idea isn't gonna work?  It would take like 100x less time to get good enough at growing that you just didn't have issues with contams than it would to actually genetically modify a species to do something that it simply doesn't have the physical structure to do.

I don't understand why the animal analogies aren't doing it for you.  You could try to breed sheep until you have aggressive sheep with claws and jaws like a bear so it could defend itself against coyotes, or you could just keep your sheep away from the fucking coyotes.  If it was me, I'd go ahead and just build a fence, since investing time in a huge improbable maybe, that could have devastating consequences on ecosystems, just seems like a lot more work.

The mycelium does not have claws and jaws to defend itself against mold. 

Can you find a way to stop mosquitos from biting you in your sleep?

"Oh well I'll just breed myself to have metal skin further down the genetic line!"

The probabilities are about the same here.  Even if it did work you'd end up with retarded looking metallic dwarf babies who don't have any friends and probably get quarantined from everybody else, just like this super strain you're talking about that would completely devastate ecology and potentially trigger the end of the world if someone didn't get it under wraps quick.  Being that it produces spores, this is a terrible deadly idea.

If that makes you mad, and makes you want to say things you might regret, you may have issues. :shrug:


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OfflinePsilosopherr
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Inocuole]
    #23554008 - 08/18/16 04:54 PM (7 years, 5 months ago)

didn't read more than a couple lines, resposting the quote again???


"Probly just another of my wacky yet useless ideas, but hopefully you at least found it interesting."


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OfflinePsilosopherr
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Psilosopherr]
    #23554018 - 08/18/16 04:57 PM (7 years, 5 months ago)

resposting in an online community with social skills :coffee:

any mod feel free to delete the post


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InvisibleMad Season
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Psilosopherr] * 1
    #23554841 - 08/18/16 08:31 PM (7 years, 5 months ago)

Quote:

bodhisatta said:





This is what happens when trich meets cubes. You'd literally have to have some sort of epic genetic mutation for it to be the other way around. And even then, to stabilize the mutation.. :justno:


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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Mad Season]
    #23554848 - 08/18/16 08:32 PM (7 years, 5 months ago)

Mad comin in with the FACTZ.

:youthemandawg:


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OfflinePsilocyBen17
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Inocuole]
    #23554865 - 08/18/16 08:38 PM (7 years, 5 months ago)

wheres that from Mad?


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