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Psilosopherr
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Vaccinating clean mushroom cultures with dead/attenuated contaminants 1
#23550395 - 08/17/16 04:13 PM (7 years, 8 months ago) |
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Been wondering if this was possible for a while now. Anybody ever heard of this concept being tested or have thoughts on why it wouldn't/might work? The only potential benefit I see it having is more resistance to contams in the grow room. I don't have a clue how immune systems in fungi compare to ours, I've simply been reading about smallpox this morning. (did you know the first known vaccination practice involved the powdering and snorting of smallpox scabs in ancient china? chinese people are fucking awesome )
The proposed procedure is simply let a petri dish sit open for a while and contaminate, then kill the contams with heat/PC or attenuate them somehow before sporulation occurs. Then aseptically add a wedge of this to a clean culture. The idea is that the byproducts produced by the contaminates will promote an immune response in the mycelium, since it has spent practically its whole life in sterile environments it might be advantageous to "get it ready for the world," before spawning to open air. 
Probly just another of my wacky yet useless ideas, but hopefully you at least found it interesting.
Edited by Psilosopherr (08/17/16 05:22 PM)
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La Flama Blanca
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Psilosopherr]
#23550401 - 08/17/16 04:15 PM (7 years, 8 months ago) |
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extremely interesting. gonna stalk this thread
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mindbentempire



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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: La Flama Blanca]
#23550582 - 08/17/16 05:12 PM (7 years, 8 months ago) |
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SoulButter
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: mindbentempire]
#23551057 - 08/17/16 07:50 PM (7 years, 8 months ago) |
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I like the thinking here. Try it out! or check google scholar for breeding resistance in fungi. I will check some time tomorrow as well.
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PsilocyBen17
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: SoulButter] 1
#23551108 - 08/17/16 08:06 PM (7 years, 8 months ago) |
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Contaminates don't ususally occur on mycelium....They occur on the substrate mycelium is trying to colonize.
Wouldn't a better idea be to train mycelium to consume mold?
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Psilosopherr
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: PsilocyBen17]
#23551190 - 08/17/16 08:27 PM (7 years, 8 months ago) |
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thats a good idea, though this may or may not accomplish the same thing
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IlBLisSlI
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: PsilocyBen17]
#23551209 - 08/17/16 08:31 PM (7 years, 8 months ago) |
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any suggestions as to how to train mycelium to accomplish such a feat? very interesting thread indeed. #following
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PsilocyBen17
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: IlBLisSlI]
#23551489 - 08/17/16 10:04 PM (7 years, 8 months ago) |
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Not sure what your getting at with the whole vacination thing..,,
Aside from training myc to consume mold you might also be able to make mycelium mold resistant by allowing it to contaminate and then isolating away from the mold for a few successive generations....but i really dont have the background for this. Its just a thought.
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Pastywhyte
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: PsilocyBen17] 1
#23551632 - 08/17/16 10:47 PM (7 years, 8 months ago) |
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If it was do easy to train cultures to eat contams do you not think that the multibillon dollar gourmet and medicinal industries would be all over that. It's like trying to train dogs to kill Burmese Pythons. Sure they might give it a good old college try. But all you end up with is dead dogs.
The easiest way to deal with contamination is to not have it.
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Psilosopherr
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Pastywhyte]
#23551709 - 08/17/16 11:10 PM (7 years, 8 months ago) |
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"If it was possible somebody would have done it by now" is not an argument I'm a fan of. Though you would think somebody would have thought of this one.
Hard to find documentation of things that don't work though
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Pastywhyte
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Psilosopherr]
#23551729 - 08/17/16 11:15 PM (7 years, 8 months ago) |
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Quote:
rbalzer said: "If it was possible somebody would have done it by now" is not an argument I'm a fan of. Though you would think somebody would have thought of this one.
Hard to find documentation of things that don't work though
What you are proposing is the figurative equivalent of creating a teleportation device powered on wishful thinking and good vibes. Sure it's impossible to rule out 100% but if it is possible, the science behind it has a looong way to go.
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Psilosopherr
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Pastywhyte]
#23551739 - 08/17/16 11:17 PM (7 years, 8 months ago) |
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even if what you say is true that's no way to explain it
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Pastywhyte
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Psilosopherr]
#23551755 - 08/17/16 11:21 PM (7 years, 8 months ago) |
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Sure there is. For instance it's very unlikely that you could ever train cubes to eat or beat trich because trich is a preditor of cubes. Trich actually fuses with the cube hyphae and destroys it from within. It's like trying to train a sheep to kill lions. Sheep just are not naturally equipped with the right physiology to take down preditors like lions.
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PsilocyBen17
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Pastywhyte]
#23551767 - 08/17/16 11:24 PM (7 years, 8 months ago) |
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oh I never said training myc to eat mold would be easy....it just seems like a better line of thought than whatever is being purposed in the OP 
Most of what I say, think, do, when it comes to mycology has already been done. And probably by someone who knows a hell of a lot more than me...
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Psilosopherr
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Pastywhyte]
#23551770 - 08/17/16 11:25 PM (7 years, 8 months ago) |
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Okay, so is there nothing resembling an immune system in fungi then? There's no difference in contam resistance between a wild strain and a lab strain?
Not trying to disagree, merely asking questions.
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PsilocyBen17
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: PsilocyBen17]
#23551779 - 08/17/16 11:27 PM (7 years, 8 months ago) |
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Your analogies are very action packed today Pasty. Dogs, Snakes, Lions. Fuck ya.
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Pastywhyte
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: PsilocyBen17]
#23551798 - 08/17/16 11:33 PM (7 years, 8 months ago) |
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Sorry Pben I wasn't really trying to bash on you either. The OP is pretty out there as well given that mycelium colonies don't really operate the same way a mammals immune system does either. They fight with enzymes and metabolites not specialized cells. Which is why we use them to take down shit our own immune systems cannot handle. But in the fungal world it's pretty clear cut. You would need to be altering the fungus on a genetic level before you would be able to get it to adapt a solution. Given how many damn species of potential competition are out there, it would be a monumental and potentially dangerous undertaking.
Godamn can ya imagine cubes that could take down all higher fungi, molds, yeasts, bacteria, and live off of just about anything? They would fuck up so many ecosystems we would be sick of seeing them.
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Pastywhyte
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Psilosopherr]
#23551803 - 08/17/16 11:34 PM (7 years, 8 months ago) |
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Quote:
rbalzer said: Okay, so is there nothing resembling an immune system in fungi then? There's no difference in contam resistance between a wild strain and a lab strain?
Not trying to disagree, merely asking questions.
Mammals immune response is mostly on a specialized cellular level. Fungi use enzymes and metabolites but lack specialized cells.
Apples and oranges.
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Psilosopherr
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Pastywhyte]
#23551814 - 08/17/16 11:39 PM (7 years, 8 months ago) |
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Well thanks for pointing out the differences between mammal and fungal immunity, coulda done without the rest of it myself.
Off to make a rage thread on another site about anti-experimentalists.
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Pastywhyte
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Psilosopherr] 1
#23551819 - 08/17/16 11:41 PM (7 years, 8 months ago) |
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Experiments have a hypothesis and are controlled. I am always enthusiastic about any thread demonstrating the use of those things. Too bad there are so few of them
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