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Milaweak
Stranger


Registered: 05/29/15
Posts: 41
Last seen: 6 years, 10 months
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Spore syringe went bacterial.. Antibiotic ?
#23550149 - 08/17/16 02:37 PM (7 years, 5 months ago) |
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Hi folks !
I tried to germinate some spores from a spore syringe but every plate looks like this :
  (Except for the agglomerate of spores on this one )
They are panaeolus.
So I was thinking of making antibacterial agar with gentamicin sulfate .
Would it help ?
Thanks !
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tito123

Registered: 01/23/10
Posts: 3,006
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Re: Spore syringe went bacterial.. Antibiotic ? [Re: Milaweak]
#23550220 - 08/17/16 03:12 PM (7 years, 5 months ago) |
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I'm not super experienced, but I've never had any luck germinating spores from syringes on agar.
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Milaweak
Stranger


Registered: 05/29/15
Posts: 41
Last seen: 6 years, 10 months
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Re: Spore syringe went bacterial.. Antibiotic ? [Re: tito123]
#23550482 - 08/17/16 04:36 PM (7 years, 5 months ago) |
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I did, a second syringe went really well.

The problem is only on one particular syringe.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Spore syringe went bacterial.. Antibiotic ? [Re: Milaweak]
#23550598 - 08/17/16 05:18 PM (7 years, 5 months ago) |
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If you wait it out, the mycelium will try to escape from the bacterial pool and after about three four or five transfers you should have a culture grade isolate.
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: Spore syringe went bacterial.. Antibiotic ? [Re: Milaweak]
#23550613 - 08/17/16 05:25 PM (7 years, 5 months ago) |
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Quote:
Milaweak said: Hi folks !
I tried to germinate some spores from a spore syringe but every plate looks like this :
  (Except for the agglomerate of spores on this one )
They are panaeolus.
So I was thinking of making antibacterial agar with gentamicin sulfate .
Would it help ?
Thanks !
That's way too many spores! Of course you're gonna see bacteria. You should not even see the spores you inoculate with, just see the points of germination. That's the goal.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Spore syringe went bacterial.. Antibiotic ? [Re: dankington]
#23550645 - 08/17/16 05:38 PM (7 years, 5 months ago) |
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Doing this dramatically helps your chances
Quote:
Trusted Cultivator said:
Quote:
Trusted Cultivator said:
Quote:
bodhisatta said:
Quote:
bodhisatta said:
take your spores to an inoculation loop. if you have a print then flame the loop dip in agar, pick spores off print and then swab the dish
if you have a syringe flame the loop and drop a few drops onto the loop to cool it down and then one more drop to load it with spores and then swab that


Quote:
bodhisatta said: Try using an inoculation loop. Flame the syringe needle and the loop then squirt onto the loop to cool the needle and the loop. Squirt another drop to prime the loop. Then swab the dish.

 
Then you can see growth immediately, see contamination easier, grab healthy mycelium easier, and never worry about spore drops rolling all over a dish giving you germination everywhere at the same time.
also the agar link in my signature
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Spore syringe went bacterial.. Antibiotic ? [Re: bodhisatta]
#23550662 - 08/17/16 05:45 PM (7 years, 5 months ago) |
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nice info and good technique
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Milaweak
Stranger


Registered: 05/29/15
Posts: 41
Last seen: 6 years, 10 months
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Re: Spore syringe went bacterial.. Antibiotic ? [Re: bodhisatta]
#23552054 - 08/18/16 01:34 AM (7 years, 5 months ago) |
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Quote:
dankington said: That's way too many spores!
I know ! But even the plates under this one where you couldn't see the spores have gone the same. It's really the syringe.
Quote:
Trusted Cultivator said: Doing this dramatically helps your chances
Thank you sir ! I need to find a inoculation loop. Would sterile cotton swaps work ?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Spore syringe went bacterial.. Antibiotic ? [Re: Milaweak]
#23552194 - 08/18/16 03:39 AM (7 years, 5 months ago) |
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Yes
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