Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineMilaweak
Stranger


Registered: 05/29/15
Posts: 41
Last seen: 6 years, 10 months
Spore syringe went bacterial.. Antibiotic ?
    #23550149 - 08/17/16 02:37 PM (7 years, 5 months ago)

Hi folks !

I tried to germinate some spores from a spore syringe but every plate looks like this :


(Except for the agglomerate of spores on this one  :rolleyes: )

They are panaeolus.

So I was thinking of making antibacterial agar with gentamicin sulfate .

Would it help ?

Thanks !


Extras: Filter Print Post Top
Invisibletito123
Male
Registered: 01/23/10
Posts: 3,006
Re: Spore syringe went bacterial.. Antibiotic ? [Re: Milaweak]
    #23550220 - 08/17/16 03:12 PM (7 years, 5 months ago)

I'm not super experienced, but I've never had any luck germinating spores from syringes on agar.


Extras: Filter Print Post Top
OfflineMilaweak
Stranger


Registered: 05/29/15
Posts: 41
Last seen: 6 years, 10 months
Re: Spore syringe went bacterial.. Antibiotic ? [Re: tito123]
    #23550482 - 08/17/16 04:36 PM (7 years, 5 months ago)

I did, a second syringe went really well.



The problem is only on one particular syringe.


Extras: Filter Print Post Top
OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Spore syringe went bacterial.. Antibiotic ? [Re: Milaweak]
    #23550598 - 08/17/16 05:18 PM (7 years, 5 months ago)

If you wait it out, the mycelium will try to escape from the bacterial pool and after about three four or five transfers you should have a culture grade isolate.


Extras: Filter Print Post Top
Invisibledankington
The Stranger
Male User Gallery


Folding@home Statistics
Registered: 03/14/15
Posts: 4,577
Loc: 8te
Re: Spore syringe went bacterial.. Antibiotic ? [Re: Milaweak]
    #23550613 - 08/17/16 05:25 PM (7 years, 5 months ago)

Quote:

Milaweak said:
Hi folks !

I tried to germinate some spores from a spore syringe but every plate looks like this :


(Except for the agglomerate of spores on this one  :rolleyes: )

They are panaeolus.

So I was thinking of making antibacterial agar with gentamicin sulfate .

Would it help ?

Thanks !




That's way too many spores! Of course you're gonna see bacteria. You should not even see the spores you inoculate with, just see the points of germination. That's the goal.


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Spore syringe went bacterial.. Antibiotic ? [Re: dankington]
    #23550645 - 08/17/16 05:38 PM (7 years, 5 months ago)

Doing this dramatically helps your chances

Quote:

Trusted Cultivator said:
Quote:

Trusted Cultivator said:
Quote:

bodhisatta said:
Quote:

bodhisatta said:


take your spores to an inoculation loop. if you have a print then flame the loop dip in agar, pick spores off print and then swab the dish

if you have a syringe flame the loop and drop a few drops onto the loop to cool it down and then one more drop to load it with spores and then swab that








Quote:

bodhisatta said:
Try using an inoculation loop. Flame the syringe needle and the loop then squirt onto the loop to cool the needle and the loop. Squirt another drop to prime the loop. Then swab the dish.



Then you can see growth immediately, see contamination easier, grab healthy mycelium easier, and never worry about spore drops rolling all over a dish giving you germination everywhere at the same time.










also the agar link in my signature







Extras: Filter Print Post Top
OfflineKenetic
Nam Sayin
 User Gallery


Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land Flag
Last seen: 5 years, 3 months
Re: Spore syringe went bacterial.. Antibiotic ? [Re: bodhisatta]
    #23550662 - 08/17/16 05:45 PM (7 years, 5 months ago)

nice info and good technique


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


Extras: Filter Print Post Top
OfflineMilaweak
Stranger


Registered: 05/29/15
Posts: 41
Last seen: 6 years, 10 months
Re: Spore syringe went bacterial.. Antibiotic ? [Re: bodhisatta]
    #23552054 - 08/18/16 01:34 AM (7 years, 5 months ago)

Quote:

dankington said:
That's way too many spores!



I know ! But even the plates under this one where you couldn't see the spores have gone the same. It's really the syringe.

Quote:

Trusted Cultivator said:
Doing this dramatically helps your chances



Thank you sir ! I need to find a inoculation loop.
Would sterile cotton swaps work ?


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Spore syringe went bacterial.. Antibiotic ? [Re: Milaweak]
    #23552194 - 08/18/16 03:39 AM (7 years, 5 months ago)

Yes


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* spore syring is in trouble? mulan 14,124 12 08/29/09 09:15 PM
by HaRo On KoRn
* Spore Syring Cont/Clear Misconcept if any Zwieback0 5,676 4 08/11/08 03:22 AM
by fr33d0mfry
* Re: When buying a spore syring... 3Mshroom 7,932 2 06/01/00 08:17 PM
by 3Mshroom
* Re: Spore transfer? Anonymous 1,901 1 03/08/01 08:17 PM
by Juggles
* how far to spore prints go? AKA PC 3,737 11 01/28/03 08:07 AM
by debianlinux
* Possible Contaminated Spore Syringe? Tim2133 2,916 5 06/30/03 11:09 PM
by Sev
* spores userdoesnotexist 1,508 5 08/06/02 07:52 AM
by mickey_rourke
* How long do spore syringes last? SixCee 5,255 4 08/01/02 10:39 PM
by mickey_rourke

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
983 topic views. 17 members, 186 guests and 21 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.01 seconds on 14 queries.