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Invisiblebilly jowl
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Registered: 12/11/12
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Colonized grain to agar
    #23549537 - 08/17/16 10:37 AM (7 years, 5 months ago)


This is probably a stupid question, but i havent read anything about it.

I have a pe grain jar fully colonized. it was made from a third transfer from agar. A small wedge, about the size of a quarter. It took about three weeks to fully colonize a quart jar.

The above pic was its recovery after a shake less than 24 hrs beforehand. I assume its pretty clean.

I was thinking if i took a few grains, put them to agar and do a few transfers to further try to clean from any possible bacteria, and get some plates with strong growth. I assume there would be no problem doing this, after all its still MS.

Then drop some agar plates to master grain jars, g2g those.
Then grow out 6 to 8 mono's, and then go from there with clones etc.

But most of all my intention is to get as close as i can to bacteria free tubs.
With hopefully a clean prolific tub or two.

Would this be a good strategy? Besides cloning my first ms grow?


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OfflineThe Mycologist
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Re: Colonized grain to agar [Re: billy jowl]
    #23549730 - 08/17/16 12:02 PM (7 years, 5 months ago)

There is no reason that this shouldn't work. All you need is one grain so its worth the try.


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"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
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InvisibleMad Season
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Re: Colonized grain to agar [Re: The Mycologist] * 1
    #23549805 - 08/17/16 12:21 PM (7 years, 5 months ago)

Yes it's been done by many, and will work


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OfflinecronicrFacebook
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Re: Colonized grain to agar [Re: Mad Season] * 1
    #23549840 - 08/17/16 12:31 PM (7 years, 5 months ago)

I do this a lot with left over grain from spawning....great leap off


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Invisiblebilly jowl
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Re: Colonized grain to agar [Re: Mad Season]
    #23549845 - 08/17/16 12:31 PM (7 years, 5 months ago)

Thanks, I figured that.
Another question I have. Ive heard is that bacteria can intermesh with the mycelium. Is this possible to spot without a microscope? Or are there tell tale signs that bacteria is present if not picked up visually?


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InvisibleMad Season
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Re: Colonized grain to agar [Re: billy jowl] * 2
    #23549855 - 08/17/16 12:35 PM (7 years, 5 months ago)



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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Invisiblebilly jowl
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Re: Colonized grain to agar [Re: Mad Season]
    #23549875 - 08/17/16 12:42 PM (7 years, 5 months ago)

Right on.
JUst what I was looking for. I cant believe Ive never ran across that thread.
Great job:thumbup:


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OfflineWill7401
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Re: Colonized grain to agar [Re: billy jowl]
    #23934786 - 12/17/16 08:36 AM (7 years, 1 month ago)

Thanks for this thread. i am getting ready to do the same thing. So what technique will you use to make the transfer of grain to agar. I've got a couple of ideas but if someone has a valid method i am eager to hear it. thanks in advance.


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OfflineSloppyJoseph
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Re: Colonized grain to agar [Re: Will7401]
    #23934810 - 12/17/16 08:53 AM (7 years, 1 month ago)

If you want the cleanest growth you take an un opened grain jar into an sab with the plate you are transferring to. Heat sterilize a pair of tweezers, and pluck one of the top grains and drop it on agar in the SAB Always practicing proper sterile technique.

Or you can do like cron said and spawn all your grain and take the extra onto agar. That been exposed to open air though and would require more clean up on agar so I don't see why you wouldn't just do the aforementioned before opening the jar up


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OfflineWill7401
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Re: Colonized grain to agar [Re: SloppyJoseph]
    #23935157 - 12/17/16 11:34 AM (7 years, 1 month ago)

Thank you. I had thought about it and yeah, flame sterilized tweezers is one of the things I thought of as well. Sounds good. I'll use that method.


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Love and Light to us all. Namaste


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InvisiblebodhisattaMDiscordReddit
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Re: Colonized grain to agar [Re: Will7401] * 1
    #23935164 - 12/17/16 11:38 AM (7 years, 1 month ago)

You could drop a colonized grain on carpet pick it up with your fingers and put it on agar and it would grow fast enough to outrun contaminants then transfer and you're golden. Any precautions taken will only help


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