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OfflineNesil
Stranger on aStrange Trip
Registered: 02/08/04
Posts: 48
Loc: Here
Last seen: 12 years, 5 months
No growth?
    #2354876 - 02/19/04 03:22 PM (12 years, 9 months ago)

Hey, everyone.  It's been about three weeks after I innoculated my first 12 jars (PF Tek), and it seems that 5 of them aren't growing.  In two or three of them, a strange, white-grey gel-like substance appears to have taken hold of the top just under the verm.  I have no idea what this is, but I'm more concerned with the lack of growth.

Is it possible that germination is occuring inside the substrate that's not visible?

Below are a few pictures...  The jars are faceted, by the way, so some of the pics may look a little strange.

White stuff

Better example of white stuff

More white stuff

No white stuff



On the other hand...  The rest


Thanks for your input.  :smirk:



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Nesil
My Shroom Movies
User: fungus, Pass: lover


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Offlinecoop
The Rookie

Registered: 01/19/04
Posts: 189
Last seen: 11 years, 7 months
Re: No growth? [Re: Nesil]
    #2354943 - 02/19/04 03:45 PM (12 years, 9 months ago)

I'm on my first growth as well and it's been about 2 1/2 weeks, and only about 1/3 of each jar is colonized. It just takes some time from what I hear. Your jars look like they are going pretty well, but I don't know about those other ones you have questions about. They look somewhat weird lol. I just opened up my incubator and pulled my jars out to take a peep. Most of them look like they are still trying, though there is this one that looks like there could be some green developing...maybe it's just me being cautious. Anyhow, just keep those whack-ass jars away from the ones that look good and hopefully you'll be fine.


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InvisibleImOver18
FormerlyMr.Sleep

Registered: 10/05/03
Posts: 763
Re: No growth? [Re: coop]
    #2355138 - 02/19/04 04:29 PM (12 years, 9 months ago)

Nesil, the jars you described with a "strange, white-grey gel-like substance" are probably contaminated. Mycelium has a distinct look, it doesn't look like anything moldy. I wouldn't throw those jars out though, I'd keep them and watch them carefully. It's very possible they have germinated and you just haven't seen it, as in it's not visible yet. Even if those few jars end up contaminated, don't sweat it. 8 colonized PF jars is still something to be happy about. Good luck.


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InvisibleZapotecorum
AlcoHoliC

Registered: 01/15/04
Posts: 186
Loc: my conversion van
Re: No growth? [Re: Nesil]
    #2355460 - 02/19/04 05:38 PM (12 years, 9 months ago)

did you shake the syringe good between jars. spores tend to sink to the tip of the syringe when inoculation casung them to be spent on the first jars. just a thought for next time.


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OfflineNesil
Stranger on aStrange Trip
Registered: 02/08/04
Posts: 48
Loc: Here
Last seen: 12 years, 5 months
Re: No growth? [Re: ImOver18]
    #2355642 - 02/19/04 06:15 PM (12 years, 9 months ago)

I was hoping the gray stuff was harmless... But I'm more than happy with the 7 jars that have grown. :-) That was my main question -- Whether it was time to throw them out or not. Thanks for the advice.

Regarding shaking the syringe, I don't think I did it after every jar... Just before I started and half-way through. But I'll definitely shake it more often next time. Can't be too careful, right?


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Nesil
My Shroom Movies
User: fungus, Pass: lover


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OfflineMex
Mother Earth
Male

Registered: 12/25/03
Posts: 256
Loc: ITA ~ Earth ~ 13:20
Last seen: 9 years, 9 months
Re: No growth? [Re: Nesil]
    #2356014 - 02/19/04 07:38 PM (12 years, 9 months ago)

Good time for throwin' away the jars?
When you start to see green / gray / yellow / ... spot
color inside them; when the smell starts to get veeery bad;
after have been posting a pic in the forum :smile:

Good luck!


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Amazon Shop for: ½ Pint Jars, Vermiculite

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