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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Set myself on fire twice using SAB?
#23546740 - 08/16/16 12:26 PM (7 years, 5 months ago) |
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Hey everyone.
So I'm inclined to believe I'm not doing something right. I set up my SAB on a tray in a small closet. Got shower, got in closet naked. Sprayed inside of SAB with alcohol. Put gloves on. Used alcohol on the gloves, put alcohol pad, syringe, jars, etc in SAB. Lit my syringe with a torch, and poof. I was engulfed in flames. My arms and hands. Luckily I had gloves on. Ripped them off. Blew all the fire out.
So, I got a gallon of water incase this happened again. Did a few jars no problem,, alcoholed the needle for each jar. 4th or 5th one later, it happened Again. I was more on fire this time. But I got the water and put it all out.
Surely you guys don't often set yourself on fire often doing this.... Am I using too much alcohol? Maybe my SAB is too small?
-------------------- KSSS And PE WBS.
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Dactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
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Re: Set myself on fire twice using SAB? [Re: Moabfighter]
#23546754 - 08/16/16 12:32 PM (7 years, 5 months ago) |
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Dude... are you flaming your needles INSIDE your SAB? Also, it isn't necessary to use alcohol on the needle if you're going to flame sterilize it. Also also, don't even bother using alcohol inside the still air box. Just spray some soapy water on the walls so the potential contaminants stick when the air settles.
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alexmir

Registered: 02/26/15
Posts: 388
Last seen: 1 year, 2 months
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Re: Set myself on fire twice using SAB? [Re: Moabfighter]
#23546756 - 08/16/16 12:33 PM (7 years, 5 months ago) |
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Alcohol is highly flammable, you should be using a bleach solution.
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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
Last seen: 4 years, 5 months
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Re: Set myself on fire twice using SAB? [Re: Moabfighter]
#23546762 - 08/16/16 12:35 PM (7 years, 5 months ago) |
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Hahaha oh man I can totally relate, I lit my whole arm on fire...second degree burns!
The vapors ate highly flammable, and if your in a small closet they probably built up and ignited lighting the residue left on you.
Wait longer in between applying the alcohol and approaching the flame. And get out of that closet!
I switched to bleach/dishsoap/water solution for inside box..and flame and or alcohol outside box
Im hoping your flame is outside of the box?
Edited by Peteyboy (08/16/16 12:38 PM)
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Set myself on fire twice using SAB? [Re: alexmir] 1
#23546763 - 08/16/16 12:35 PM (7 years, 5 months ago) |
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Shit wtf am I doing.
Didn't wanna sound like a dumbass asking if alcohol was the thing to use, because I've used it often in the past just never really used a SAB much.
Yes flaming inside the box...
I burned all my arm hairs off.
-------------------- KSSS And PE WBS.
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rider420
Ghost in the machine


Registered: 02/11/16
Posts: 659
Last seen: 4 months, 7 days
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Re: Set myself on fire twice using SAB? [Re: Moabfighter]
#23546765 - 08/16/16 12:36 PM (7 years, 5 months ago) |
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DON'T use a lighter with this method! Next time use a clear plastic bag the kind you recycle in. Put the alcohol spray bottle in the bag with the syringe and jars and tie it air tight shut leave it only half full of air so you can use the corners as gloves. Spray everything down with the 99% alcohol including the bag, jars and syringes from the outside by using the corners as gloves. Then wait 15 min then using the corners as gloves inoculate the jars, only when finished open the bag up and take out the now inoculated jars and empty syringes. Went from 20 to 40 contamination to 0% every time!
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Set myself on fire twice using SAB? [Re: Moabfighter]
#23546766 - 08/16/16 12:37 PM (7 years, 5 months ago) |
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I was under the impression every movement I made had to be done inside the cramped SAB. Removing foil from lids, wiping the jars down with the wipe to clean them and the lids.
Was just trying to be as sterile as I thought I could be. Almost burned the house down. Twice.
-------------------- KSSS And PE WBS.
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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
Last seen: 4 years, 5 months
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Re: Set myself on fire twice using SAB? [Re: Moabfighter]
#23546772 - 08/16/16 12:39 PM (7 years, 5 months ago) |
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ALWAYS flame outside of box...will solve all your problems...as far as lighting yourself on fire goes lol
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Set myself on fire twice using SAB? [Re: Peteyboy]
#23546779 - 08/16/16 12:42 PM (7 years, 5 months ago) |
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Well shit. I did 6 jars all flaming inside the box.
Didn't bring no bullshit in there haha, maybe these will turn out good.
-------------------- KSSS And PE WBS.
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Dactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
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Re: Set myself on fire twice using SAB? [Re: Moabfighter]
#23546784 - 08/16/16 12:43 PM (7 years, 5 months ago) |
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It's all good dude. The heat from the flame actually creates an air current within the still air box so it's not only a fire hazard but it raises your chances of contamination. If you're flaming your needles until they're red hot nothing that touches them on their way into the still air box will survive. Also, disregard that advice about the bag. If anything you should be using 70% alcohol to sanitize. It's to my understanding the extra water content helps penetrate the cell walls of the nasties. Everything you're doing is fine, just get that flame out of there and spray your box down with soapy water before use next time.
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themadextractor
Stranger


Registered: 06/25/14
Posts: 230
Last seen: 1 year, 3 months
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Re: Set myself on fire twice using SAB? [Re: Dactylium]
#23546811 - 08/16/16 12:52 PM (7 years, 5 months ago) |
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Dude. You use soapy water in a spray bottle. alcohol vapors ignite and don't sterilize anything. Youre tying to trap contaminants to the sides. Not sterilize your box. Switch to soapy water and you won't see fire every time you use your box and will fight contams much better
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Psykronik
OverGrower


Registered: 02/28/12
Posts: 150
Loc: Scotland
Last seen: 7 years, 4 months
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Re: Set myself on fire twice using SAB? [Re: Dactylium]
#23546822 - 08/16/16 12:55 PM (7 years, 5 months ago) |
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Your not looking to sanitise or sterilise the inside of an SAB. Your looking to put some form of way for airborne particles and such to stick to the walls of the SAB and the floor. You don't even need bleach... I don't know where people are reading this info. Just water or soapy water sprayed inside the SAB. I use a towel on the bottom but only to properly seal my SAB. I also spray the towel so as its slightly damp to make the particles stick to it and not fly around the SAB when moving things around.
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Re: Set myself on fire twice using SAB? [Re: Dactylium]
#23546832 - 08/16/16 12:58 PM (7 years, 5 months ago) |
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People who don't understand how flammable vapors work shouldn't be working with products that create flammable vapors.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Set myself on fire twice using SAB? [Re: Juiceh]
#23546869 - 08/16/16 01:12 PM (7 years, 5 months ago) |
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This whole thread is filled with crazy info.
Op, you do not need to sanitize your sab, so alcohol is useless. I use a soapy water concoction i cooked up by putting soap in water Before sab work, spray all the walls and floor of your sab and wait 15 or so minutes for the air to settle then get to work.
The sab works on the concept of still air not sanitation.
Also, it should go without saying that you flame sterilize OUTSIDE your sab and, while still red hot, bring your tool inside the sab to cool. If you sterilize inside the box, heat vapors created by the lighter stir up the air inside, defeating its purpose.
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morty422
Wuba-luba-dub-dub!



Registered: 07/06/16
Posts: 988
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Re: Set myself on fire twice using SAB? [Re: Juiceh] 1
#23546875 - 08/16/16 01:14 PM (7 years, 5 months ago) |
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Quote:
Juiceh said: People who don't understand how flammable vapors work shouldn't be working with products that create flammable vapors.

Hahaha.
Moab, this post is awesome. Thanks man!
I'm over here worried about my monotub starting to fruit and you're lighting yourself on fire! Hahah
Not intending to troll you or anything, this was just a breathe of fresh air kind of thread... lol
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Set myself on fire twice using SAB? [Re: natedawgnow]
#23546877 - 08/16/16 01:16 PM (7 years, 5 months ago) |
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Interesting info. I can pick through the good and the bad. Honest mistake. Should've researched better.
And yeah no harm no foul. Could've been really bad, but turned out alright.
-------------------- KSSS And PE WBS.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Set myself on fire twice using SAB? [Re: morty422]
#23546889 - 08/16/16 01:22 PM (7 years, 5 months ago) |
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If I was OP I wouldn't have admitted to this in a thread. lol
Fuck.... All you have to do is use the damn search engine
Quote:
MudaFuka said: Dish soap and water. I used to use alcohol but it's to expensive and dries to quickly to do the job properly. Water and soap work great to trap contaminants on the floor and walls of your SAB.
Quote:
SpitballJedi said:
Quote:
cc1 said: but I put Lysol in it mostly and let it sit for at least 10 min. And that seems to be working great, but if I am wasting money , as in of soapy water spray works, I need to give it a shot, I just hate to fuck something up as I know the Lysol works,
I used to buy Lysol and Oust and what-not, but I discovered it to be a waste of money.
The soap and water in a spray bottle is very effective because sterility is not required in a SAB. You just want the air to be still so nothing will drift in to your sterile media.
The reason I use soap water is because it doesn't dry up fast. Contams will stick to the wet surfaces. I don't take much. Also the mist attaches to some of the particles and drags them down where they stick to the damp surface.
Lysol, alcohol, bleach, Oust, or whatever isn't any more affective in this. I wipe my SAB down occasionally, but being anal is not required.
I do G2G and agar work in mine and rarely get contamination. It took me a long time to realize no chemical will ever make up for sloppy technique.
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PussyFart said: Your SAb does not have to be sterile, or even sanitary....it just has to provide a draft free environment.
Wetting the walls with anything will work....
And chemicals sanitize, heat sterilizes.
We cannot sterilize our SABs....
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RogerRabbit said: That's a joke right?
A plastic bag without the wind tunnel machine would work far better. You want still air. RR
Quote:
RogerRabbit said: Use still air, or buy a laminar flow hood. Don't attempt to put a fan and/or filter on a glovebox or you'll increase rate of contamination.
You also don't spray lysol in a glovebox. Use soap and water, and you can spray the inside with plain water to trap and hold contaminants while you work. RR
Quote:
TranscendingLife said:
I don't fix my gloves to my SAB ever...It gives you more freedom w/ in the SAB...dude, seriously, you need to check the SAB in my sig...I'm telling you. It's exact design is from RR (not personally, but my study of his SAB in his videos)
Please don't discredit those of us w/ tried & true methods. We all know RR is a guru on here, but we all have valuable advice...
Quote:
RogerRabbit said: A fan is the worst possible thing you can do to a glovebox. There is also no need to spray lysol or oust in a GB. Wipe it with a damp cloth and go to work. There is nothing sterile about a glovebox. STILL air is what you want. RR
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Roadkill said: Still air is what you want!~

mine.... http://www.shroomery.org/forums/showflat.php/Number/4359637
tc
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RogerRabbit said: I would never recommend 'open air' anything related to inoculations of grains, petri dishes, or even pf cakes. It's just too easy to build a simple still air box as shown in more than one post above. RR
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13shrooms said: as long as the air inside the box was still, you can achieve many things with very little as long as your sterile tek is good, if you werent going in and out of your box and worked quickly but smoothly (not having to take your arms in and out) your good. 
you only need to wet the walls with a lil spray of soapy water in your GB. the whole reason to do that is so if there are any airborn contams they will stick to the sides/bottom of the GB and not land in your work, not to "sterilize/sanitize the GB. its good to wipe it out with iso alc but wet it down w/soapy water for best results.
Also
https://www.shroomery.org/forums/showflat.php/Number/20048771
Very first result
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Bikerfool
Your Local Edgelord


Registered: 11/21/05
Posts: 1,577
Last seen: 5 months, 14 days
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Re: Set myself on fire twice using SAB? [Re: bodhisatta]
#23547023 - 08/16/16 02:16 PM (7 years, 5 months ago) |
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So.... You're doing your inoculations naked?
-------------------- Just an angsty teen contributing to the pubs decline with contentless posts.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Set myself on fire twice using SAB? [Re: Bikerfool]
#23547040 - 08/16/16 02:22 PM (7 years, 5 months ago) |
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Quote:
Psykronik said: Your not looking to sanitise or sterilise the inside of an SAB. Your looking to put some form of way for airborne particles and such to stick to the walls of the SAB and the floor. You don't even need bleach... I don't know where people are reading this info. Just water or soapy water sprayed inside the SAB. I use a towel on the bottom but only to properly seal my SAB. I also spray the towel so as its slightly damp to make the particles stick to it and not fly around the SAB when moving things around.
.......
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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theGODSmademedoit

Registered: 03/04/16
Posts: 516
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Re: Set myself on fire twice using SAB? [Re: Moabfighter]
#23547583 - 08/16/16 05:34 PM (7 years, 5 months ago) |
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Quote:
Moabfighter said: Hey everyone.
So I'm inclined to believe I'm not doing something right. I set up my SAB on a tray in a small closet. Got shower, got in closet naked. . ....bro you know you can put clothes on right.lmao
Edited by theGODSmademedoit (08/16/16 05:35 PM)
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