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Offlinedonkeycong
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Registered: 05/05/14
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How do you know if Agar plates are contaminated or not?
    #23543497 - 08/15/16 10:28 AM (7 years, 5 months ago)

I inoculated some agar plates using prints. It seems that the most common way to tell if the plates are clean is to see the organization of the growth on the plate. Since I used prints and don't want to isolate how would I tell if the plates are clean if there isn't much organization while using spores right away anyway? Here are some pics of what stage they're at:



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InvisiblebodhisattaMDiscordReddit
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Re: How do you know if Agar plates are contaminated or not? [Re: donkeycong]
    #23543529 - 08/15/16 10:36 AM (7 years, 5 months ago)

You want to takenat least 1-2 more transfers from the original plate that got spores anyway.

Even if it's clean in appearance you don't want to bring along any contamination that may not have germinated that could have been in your spores.

Take from the leading edge to a new plate. It will be much easier to tell on plate 2 or 3.


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