Home | Community | Message Board

Cannabis Seeds - Original Sensible Seeds
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals CBD Concentrates   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   PhytoExtractum Kratom Powder for Sale   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineDeadPhan
Male User Gallery


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
Is bacteria a given with consistent high Temps on inoculated grain jars?
    #23542988 - 08/15/16 05:57 AM (7 years, 5 months ago)

In the past I usually store jars inside but I have had to store mine lately outside. In shade and all but with this heat wave Temps getting upwards to 100+ and crazy humidity, (I want to kill the sun) all my jars have gone to shit. I should of known better and found somewhere else to let them colonize but figured, clean master jar, whatever. But they all went to shit and I can't help but wonder that no matter how clean your spawn or master jar is, if it's too hot they will contam regardless. Do you think I may have fucked up along the way with my sterile technique?  Because, I was otherwise having a good run of clean spawn from transfers up until this heat wave. What gives if heat makes a sterile enough jar of spawn to go bad?  Is that the likely culprit. In which case till this bullshit heat subsides I'll find a better place to store. I gotta go back to agar again which is ok but Definitly setting me back. I had clean spawn but they all got bacteria now. I got shit in the fridge but I'm not gonna g2g them. Throw some kernels on agar and all that. So it goes. I was just wondering if being to hot almost always makes for bacterial contamed spawn.


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


Extras: Filter Print Post Top
Offlineinvitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 21 days
Re: Is bacteria a given with consistent high Temps on inoculated grain jars? [Re: DeadPhan]
    #23543013 - 08/15/16 06:22 AM (7 years, 5 months ago)

good question


Extras: Filter Print Post Top
Invisiblepablokabute
Hari ng Amag
 User Gallery


Registered: 11/22/11
Posts: 5,163
Loc: rural ghetto
Re: Is bacteria a given with consistent high Temps on inoculated grain jars? [Re: invitro]
    #23543087 - 08/15/16 07:15 AM (7 years, 5 months ago)

plausible


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..


Extras: Filter Print Post Top
OfflineIkarus
Whooooshhh

Folding@home Statistics
Registered: 06/13/14
Posts: 61
Last seen: 6 years, 5 months
Re: Is bacteria a given with consistent high Temps on inoculated grain jars? [Re: pablokabute]
    #23543156 - 08/15/16 08:01 AM (7 years, 5 months ago)

100 degree heat, plus any heat your mycelium was generating in the jar, and I'd say the mycelium is struggling just to survive, if it isn't dying off already. Any bacterial spores that survived sterilization are probably having free run.


Extras: Filter Print Post Top
Offlinetommyg57

Registered: 07/05/15
Posts: 140
Loc: Under the stars Flag
Last seen: 6 years, 7 months
Re: Is bacteria a given with consistent high Temps on inoculated grain jars? [Re: Ikarus]
    #23543175 - 08/15/16 08:18 AM (7 years, 5 months ago)

I'm very curious to this as well. I just lost 3 out of 8 jars to contams. The three jars were covered in a grey mold, almost half the grain. Its also the warmest I think I've had them colonize.. Temps were 79-85 outside jar temp for a week. So very curious to see what some TC's and vets have to say:thumbup:


Extras: Filter Print Post Top
InvisibleCosmoKramer
The Assman

Registered: 06/22/16
Posts: 555
Re: Is bacteria a given with consistent high Temps on inoculated grain jars? [Re: Ikarus]
    #23543352 - 08/15/16 09:45 AM (7 years, 5 months ago)

Quote:

Ikarus said:
100 degree heat, plus any heat your mycelium was generating in the jar, and I'd say the mycelium is struggling just to survive, if it isn't dying off already. Any bacterial spores that survived sterilization are probably having free run.



:whathesaid:

Lower temps are recommended for a reason. When you start going above 80f the ratio of contamination just increases more and more.


--------------------
"Get yourself some vitamin C with rose hips and bioflavonoids."


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Bridgetown Botanicals CBD Concentrates   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   PhytoExtractum Kratom Powder for Sale   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* whole grain jars last?.................... Shadowfoot 1,439 1 10/29/02 04:17 PM
by SixTango
* When to case grain jars shianggu 3,182 1 04/30/02 07:23 AM
by humplok4201
* Grain jars not PCd wikedanjel 1,047 5 01/16/03 07:39 AM
by Azmodeus
* Foamy yellow liquid in Rye Grain jar Visigoth 3,241 3 10/14/02 08:45 AM
by MAIA
* Finished Grain jars, having a few questions... wikedanjel 1,672 2 01/17/03 05:27 AM
by debianlinux
* First Time Grower: Day 16 : Grain Jar Transfer Questions, 'Howmidoin?' Gordon Shumway 1,025 8 05/07/21 05:39 PM
by Gordon Shumway
* Re: temp of incubating jars AnubisRonin 2,388 9 01/27/00 10:27 AM
by Lana
* Do i need to cap my grain jars while autoclaving? darshan 2,349 12 09/10/02 04:38 PM
by darshan

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
424 topic views. 17 members, 198 guests and 25 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.007 seconds on 12 queries.