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DeadPhan



Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Is bacteria a given with consistent high Temps on inoculated grain jars?
#23542988 - 08/15/16 05:57 AM (7 years, 5 months ago) |
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In the past I usually store jars inside but I have had to store mine lately outside. In shade and all but with this heat wave Temps getting upwards to 100+ and crazy humidity, (I want to kill the sun) all my jars have gone to shit. I should of known better and found somewhere else to let them colonize but figured, clean master jar, whatever. But they all went to shit and I can't help but wonder that no matter how clean your spawn or master jar is, if it's too hot they will contam regardless. Do you think I may have fucked up along the way with my sterile technique? Because, I was otherwise having a good run of clean spawn from transfers up until this heat wave. What gives if heat makes a sterile enough jar of spawn to go bad? Is that the likely culprit. In which case till this bullshit heat subsides I'll find a better place to store. I gotta go back to agar again which is ok but Definitly setting me back. I had clean spawn but they all got bacteria now. I got shit in the fridge but I'm not gonna g2g them. Throw some kernels on agar and all that. So it goes. I was just wondering if being to hot almost always makes for bacterial contamed spawn.
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 21 days
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Re: Is bacteria a given with consistent high Temps on inoculated grain jars? [Re: DeadPhan]
#23543013 - 08/15/16 06:22 AM (7 years, 5 months ago) |
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good question
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,163
Loc: rural ghetto
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Re: Is bacteria a given with consistent high Temps on inoculated grain jars? [Re: invitro]
#23543087 - 08/15/16 07:15 AM (7 years, 5 months ago) |
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plausible
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Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
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Ikarus
Whooooshhh


Registered: 06/13/14
Posts: 61
Last seen: 6 years, 5 months
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Re: Is bacteria a given with consistent high Temps on inoculated grain jars? [Re: pablokabute]
#23543156 - 08/15/16 08:01 AM (7 years, 5 months ago) |
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100 degree heat, plus any heat your mycelium was generating in the jar, and I'd say the mycelium is struggling just to survive, if it isn't dying off already. Any bacterial spores that survived sterilization are probably having free run.
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tommyg57

Registered: 07/05/15
Posts: 140
Loc: Under the stars
Last seen: 6 years, 7 months
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Re: Is bacteria a given with consistent high Temps on inoculated grain jars? [Re: Ikarus]
#23543175 - 08/15/16 08:18 AM (7 years, 5 months ago) |
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I'm very curious to this as well. I just lost 3 out of 8 jars to contams. The three jars were covered in a grey mold, almost half the grain. Its also the warmest I think I've had them colonize.. Temps were 79-85 outside jar temp for a week. So very curious to see what some TC's and vets have to say
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CosmoKramer
The Assman

Registered: 06/22/16
Posts: 555
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Re: Is bacteria a given with consistent high Temps on inoculated grain jars? [Re: Ikarus]
#23543352 - 08/15/16 09:45 AM (7 years, 5 months ago) |
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Quote:
Ikarus said: 100 degree heat, plus any heat your mycelium was generating in the jar, and I'd say the mycelium is struggling just to survive, if it isn't dying off already. Any bacterial spores that survived sterilization are probably having free run.

Lower temps are recommended for a reason. When you start going above 80f the ratio of contamination just increases more and more.
-------------------- "Get yourself some vitamin C with rose hips and bioflavonoids."
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