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InvisibleMad Season
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Registered: 09/16/12
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Re: Let's see what is inside of coir [Re: Rooster Cogburn]
    #23549393 - 08/17/16 09:31 AM (7 years, 5 months ago)

Quote:

Rooster Cogburn said:
Alright guys I have some updates
The only samples to get mold which in this case is cobweb is the dry and the hydrated, the hydrated plate filled up in less than 24 hours from not seeing anything, but I just noticed faint wisps of it's mycelium on the dry sample, I couldnt get a picture good enough to see it, but it's there and will probably be able to get a good picture of tomorrow.

Here is the hydrated sample, see the mold


And here is the dry sample


The pasteurized sample


And the bucket/boiling water treated sample


The hot water treated coir seems to have the fewest colonies out of all of them.
Im going to see If I can get some better pics of the mold in few minutes



:awedance: pretty cool shit. It's cool that the hydrated coir is developing the most shit, and even a mold


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OfflineKenetic
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Re: Let's see what is inside of coir [Re: bodhisatta]
    #23550239 - 08/17/16 03:18 PM (7 years, 5 months ago)

Quote:

Trusted Cultivator said:
then you can enumerate CFU/g of coir

say you use 100mL of water in a jar and sterilize it then weigh it and it weighs 300 grams we assume 100g is water. then add coir. you weigh it after adding coir and it's 310g. so we know 10g of coir in 100mL water.

we plate 1mL of coir water in a pour plate.

then if you get any colonies in the plate since you did a pour plate you can count the colonies.

then say you counted 10 colonies. you would have 10cfu per 1ml and 1ml has .1g coir since there's 10g in 100mL





CFU calulation:  #of CFU/(dilution plated*dilution factor) = #of CFU/gram

CFU = colony-forming unit, and only applies to viable cells.

It has a lot of reasons to be useful in mycology, like knowing the severity of a contaminate problem before and after a period of time, whether or not your anti-contaminate countermeasures are working or getting worse, and making beer.  Which is really useful in mycology imo.  Cheers!


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OfflineRooster Cogburn
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Registered: 07/19/16
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Re: Let's see what is inside of coir [Re: Kenetic]
    #23550532 - 08/17/16 04:51 PM (7 years, 5 months ago)

Thanks Mad. :super:

Hey Pasty, the mold is rhizopus for sure, the ends of the myc turned green today as well the dry sample took off with rhizopus like crazy. I'll get some pictures later.


Edited by Rooster Cogburn (08/17/16 04:58 PM)


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InvisibleComebackKid
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Registered: 05/27/16
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Re: Let's see what is inside of coir [Re: Rooster Cogburn]
    #23550578 - 08/17/16 05:10 PM (7 years, 5 months ago)

Frig this makes me glad I learned about the bucket tek before just hydrating and givin'er which was my original plan...


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InvisiblePastywhyteMDiscord
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Re: Let's see what is inside of coir [Re: ComebackKid]
    #23551116 - 08/17/16 08:07 PM (7 years, 5 months ago)

Quote:

ComebackKid said:
Frig this makes me glad I learned about the bucket tek before just hydrating and givin'er which was my original plan...




Except it won't hurt to hydrate and giver. Coir is a poor food source for bacteria and mold can't germinate on it. If not for the agar media those CFU'S wouldn't stand a chance to get a foothold. Only thing that would suffer would be yield.


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InvisibleComebackKid
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Re: Let's see what is inside of coir [Re: Pastywhyte]
    #23552329 - 08/18/16 06:05 AM (7 years, 5 months ago)

Science bitch! Okay that makes sense. Nutes would usually already be taken up in the spawn :congrats:


--------------------
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Look around you... Everything you see exists inside the mind.
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Our individual experiences are all part of the universe's experience of itself


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