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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Quote:
Rooster Cogburn said: Alright guys I have some updates The only samples to get mold which in this case is cobweb is the dry and the hydrated, the hydrated plate filled up in less than 24 hours from not seeing anything, but I just noticed faint wisps of it's mycelium on the dry sample, I couldnt get a picture good enough to see it, but it's there and will probably be able to get a good picture of tomorrow.
Here is the hydrated sample, see the mold

And here is the dry sample

The pasteurized sample

And the bucket/boiling water treated sample

The hot water treated coir seems to have the fewest colonies out of all of them. Im going to see If I can get some better pics of the mold in few minutes
pretty cool shit. It's cool that the hydrated coir is developing the most shit, and even a mold
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Let's see what is inside of coir [Re: bodhisatta]
#23550239 - 08/17/16 03:18 PM (7 years, 5 months ago) |
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Quote:
Trusted Cultivator said: then you can enumerate CFU/g of coir
say you use 100mL of water in a jar and sterilize it then weigh it and it weighs 300 grams we assume 100g is water. then add coir. you weigh it after adding coir and it's 310g. so we know 10g of coir in 100mL water.
we plate 1mL of coir water in a pour plate.
then if you get any colonies in the plate since you did a pour plate you can count the colonies.
then say you counted 10 colonies. you would have 10cfu per 1ml and 1ml has .1g coir since there's 10g in 100mL
CFU calulation: #of CFU/(dilution plated*dilution factor) = #of CFU/gram
CFU = colony-forming unit, and only applies to viable cells.
It has a lot of reasons to be useful in mycology, like knowing the severity of a contaminate problem before and after a period of time, whether or not your anti-contaminate countermeasures are working or getting worse, and making beer. Which is really useful in mycology imo. Cheers!
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Re: Let's see what is inside of coir [Re: Kenetic]
#23550532 - 08/17/16 04:51 PM (7 years, 5 months ago) |
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Thanks Mad.
Hey Pasty, the mold is rhizopus for sure, the ends of the myc turned green today as well the dry sample took off with rhizopus like crazy. I'll get some pictures later.
Edited by Rooster Cogburn (08/17/16 04:58 PM)
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Frig this makes me glad I learned about the bucket tek before just hydrating and givin'er which was my original plan...
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Let's see what is inside of coir [Re: ComebackKid]
#23551116 - 08/17/16 08:07 PM (7 years, 5 months ago) |
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Quote:
ComebackKid said: Frig this makes me glad I learned about the bucket tek before just hydrating and givin'er which was my original plan...
Except it won't hurt to hydrate and giver. Coir is a poor food source for bacteria and mold can't germinate on it. If not for the agar media those CFU'S wouldn't stand a chance to get a foothold. Only thing that would suffer would be yield.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Let's see what is inside of coir [Re: Pastywhyte]
#23552329 - 08/18/16 06:05 AM (7 years, 5 months ago) |
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Science bitch! Okay that makes sense. Nutes would usually already be taken up in the spawn
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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