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CosmoKramer
The Assman

Registered: 06/22/16
Posts: 555
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Re: Let's see what is inside of coir [Re: Pastywhyte]
#23545673 - 08/15/16 11:10 PM (7 years, 5 months ago) |
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Cool experimental run! 24-48hrs more and they will show if present.
-------------------- "Get yourself some vitamin C with rose hips and bioflavonoids."
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Let's see what is inside of coir [Re: CosmoKramer] 1
#23545758 - 08/15/16 11:47 PM (7 years, 5 months ago) |
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I'm off third shift in a week forever. So I can actually do shit I'll try this too I think
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Let's see what is inside of coir [Re: bodhisatta] 1
#23545980 - 08/16/16 04:26 AM (7 years, 5 months ago) |
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Yeah, definitely should have tried the PCed coir with this. As it sits, you can't be sure if it's your tools or sterile tek that's contaminating your samples. The controls you showed is simply a poured plate, not one that was used for transfers so in this case, it's only showing you that the agar you started off with was clean but nothing regarding anything that happened after.
It would be good to explain your method of obtaining the coir from the inside of the brick and your pre-cautions to make sure you do not contaminate it while getting the sample. Even with the PCed coir control, it wouldn't totally confirm that the dry sample was obtained with sterility, since you are going to be splitting your brick and taking a sample while the PCed sample is coming straight from a sterile jar.
This seems like a simple task but once you start thinking of the shit that can go wrong, you realise it's not as simple as you first thought it would be.
Quote:
Trusted Cultivator said: I'm off third shift in a week forever. So I can actually do shit I'll try this too I think
Now this I would be very interested in, I think the credibility of the user's capability is paramount in such an experiment . Can't really question someone who does this stuff for a living now, can you?
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mindbentempire



Registered: 10/26/09
Posts: 258
Loc: Australia
Last seen: 16 days, 23 hours
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Re: Let's see what is inside of coir [Re: Supalemonhaze]
#23546108 - 08/16/16 06:44 AM (7 years, 5 months ago) |
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Quote:
Supalemonhaze said: Yeah, definitely should have tried the PCed coir with this. As it sits, you can't be sure if it's your tools or sterile tek that's contaminating your samples. The controls you showed is simply a poured plate, not one that was used for transfers so in this case, it's only showing you that the agar you started off with was clean but nothing regarding anything that happened after.
It would be good to explain your method of obtaining the coir from the inside of the brick and your pre-cautions to make sure you do not contaminate it while getting the sample. Even with the PCed coir control, it wouldn't totally confirm that the dry sample was obtained with sterility, since you are going to be splitting your brick and taking a sample while the PCed sample is coming straight from a sterile jar.
This seems like a simple task but once you start thinking of the shit that can go wrong, you realise it's not as simple as you first thought it would be.
Quote:
Trusted Cultivator said: I'm off third shift in a week forever. So I can actually do shit I'll try this too I think
Now this I would be very interested in, I think the credibility of the user's capability is paramount in such an experiment . Can't really question someone who does this stuff for a living now, can you?
I agree, there can be lots of confounding variables that might skew the results here. But the OP is doing awesome work here. Any documented experiments will be good. Others can expand and fine tune based on the discussions this brings about.
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Re: Let's see what is inside of coir [Re: Supalemonhaze]
#23546910 - 08/16/16 01:27 PM (7 years, 5 months ago) |
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Quote:
Supalemonhaze said: Yeah, definitely should have tried the PCed coir with this. As it sits, you can't be sure if it's your tools or sterile tek that's contaminating your samples. The controls you showed is simply a poured plate, not one that was used for transfers so in this case, it's only showing you that the agar you started off with was clean but nothing regarding anything that happened after.
It would be good to explain your method of obtaining the coir from the inside of the brick and your pre-cautions to make sure you do not contaminate it while getting the sample. Even with the PCed coir control, it wouldn't totally confirm that the dry sample was obtained with sterility, since you are going to be splitting your brick and taking a sample while the PCed sample is coming straight from a sterile jar.
This seems like a simple task but once you start thinking of the shit that can go wrong, you realise it's not as simple as you first thought it would be.
Quote:
Trusted Cultivator said: I'm off third shift in a week forever. So I can actually do shit I'll try this too I think
Now this I would be very interested in, I think the credibility of the user's capability is paramount in such an experiment . Can't really question someone who does this stuff for a living now, can you?
Thank you for that absolutely enlightening contribution to this discussion
The dry and hydrated samples were obtained by opening a fresh packaged 5kg brick and splitting it open all in the lab, the packaging was even cleaned well before bringing into the lab which had been scrubbed for hours ahead of time, not for this but for the agar session, and then transferred to a baggie with out touching it. Now haze, here is a trick for clean tools, you sterilize them. Now while doing work you flame sterilize the tools like your scalpel between transfers.
Haze, understand this is just the start, of course we are going to weed out the variables, and add sterilized coir. And thanks to Bohdi we are going to be taking the experiment to pour plates and start counting the CFU's. What's with the hatin' man? This is going to be both fun and informative. Never stop trying to learn Haze, that's what takes us places we never thought we would go and brings a certain sense of humbleness.
Edited by Rooster Cogburn (08/16/16 03:15 PM)
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Quote:
Rooster Cogburn said:
Now haze, here is a trick for clean tools, you sterilize them. Now while doing work you flame sterilize the tools like your scalpel between transfers.
Ah, so that's how the pros do it. 
Hating? Where is this hating?
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Re: Let's see what is inside of coir [Re: Supalemonhaze]
#23548111 - 08/16/16 08:29 PM (7 years, 5 months ago) |
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Again, nice contribution to the subject
Grade A content, and as far as the hatin' goes maybe it's just the attitude, kind of unfriendly.
"Ah, so that's how the pros do it. "
What's up with that?
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Alright guys I have some updates The only samples to get mold which in this case is cobweb is the dry and the hydrated, the hydrated plate filled up in less than 24 hours from not seeing anything, but I just noticed faint wisps of it's mycelium on the dry sample, I couldnt get a picture good enough to see it, but it's there and will probably be able to get a good picture of tomorrow.
Here is the hydrated sample, see the mold

And here is the dry sample

The pasteurized sample

And the bucket/boiling water treated sample

The hot water treated coir seems to have the fewest colonies out of all of them. Im going to see If I can get some better pics of the mold in few minutes
Edited by Rooster Cogburn (08/16/16 11:12 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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I doubt that is dactyllium. Probably a rhizopus sp. Still pretty interesting.
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Re: Let's see what is inside of coir [Re: Pastywhyte]
#23548514 - 08/16/16 11:16 PM (7 years, 5 months ago) |
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I think your right, I just compared their pictures. Hoping to get better photos of it.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Quote:
Rooster Cogburn said: Again, nice contribution to the subject
Grade A content, and as far as the hatin' goes maybe it's just the attitude, kind of unfriendly.
"Ah, so that's how the pros do it. "
What's up with that?
What's up with that? Really? How else do you imagine people sterilizing their tools? I mean, if you really can't recognize humour when you see it, it's no wonder I am "hating".
Hmm, nevermind, I just noticed your postcount. You still have to learn that on the shroomery, you have to leave the ego at the door.
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Re: Let's see what is inside of coir [Re: Supalemonhaze]
#23548720 - 08/17/16 01:25 AM (7 years, 5 months ago) |
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Where am I giving off the impression I have a big ego
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
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Re: Let's see what is inside of coir [Re: bodhisatta] 1
#23548743 - 08/17/16 01:43 AM (7 years, 5 months ago) |
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--------------------
Highwalker
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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On it, gorilla glue , ho!
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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That's what's been floating around my part of the woods too.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Because every reply I posted in this thread seemed to rub you the wrong way, even the one where it's clearly a joke.
Quote:
What's with the hatin' man?
Quote:
Supalemonhaze said:
Quote:
Rooster Cogburn said:
Now haze, here is a trick for clean tools, you sterilize them. Now while doing work you flame sterilize the tools like your scalpel between transfers.
Ah, so that's how the pros do it. 
Quote:
Rooster Cogburn said: Again, nice contribution to the subject
Grade A content, and as far as the hatin' goes maybe it's just the attitude, kind of unfriendly.
"Ah, so that's how the pros do it. "
What's up with that?

But OK.... I guess.
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Re: Let's see what is inside of coir [Re: Supalemonhaze]
#23548806 - 08/17/16 02:32 AM (7 years, 5 months ago) |
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This was stupid shit, let's move on from it, take a with us while we are at it and tell us what you think is going on with the plates. I love this place, I'm not planning on leaving anytime soon so let's just get the fuck along
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Re: Let's see what is inside of coir [Re: bodhisatta]
#23548814 - 08/17/16 02:37 AM (7 years, 5 months ago) |
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Quote:
Trusted Cultivator said: That's what's been floating around my part of the woods too.
It's almost ALL of what's been around here, I like it though.
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