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Anonymous

Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: TheDrugStore]
    #2370110 - 02/23/04 12:32 AM (15 years, 4 months ago)

Overlay is a hardened mass of mycelium. It can prevent the mycelium from taking up water or breathing.

I would mist and increase fanning. Don't case again, you will only delay pin set. Pins should form 5-9 days from casing. You just overincubated them a bit, NOT ENOUGH FANNING. They are still growing vegetatively, and casing again will just perpetuate that. Just mist and fan, and await pins shortly.


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Invisiblesci33
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Registered: 11/11/03
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: ]
    #2370151 - 02/23/04 12:47 AM (15 years, 4 months ago)

ack, I sort of gave in before I read your reply and placed a thin layer on top of them earlier in the day.. oh well.

Anyhow, I hooked up a cool mist humidifier to the chamber on a timer, and it will exchange air every hour in addition to the fanning. Hopefully that will do it.


--------------------
Looking for:
Ps. weilii, Ps. samuiensis, Ps. hispanica, Pan. cyanescens (any strain I don't have)

Have:
Ps. cubensis, Ps. tampanensis, Pan. cyanescens (Australian, Tai-KS, Hawaiian, Jamaican, Ban Lipa Noi)

PM for a trade.


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InvisibleLoki
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: sci33]
    #2370592 - 02/23/04 03:00 AM (15 years, 4 months ago)

Great thread, thank you.
And good luck :thumbup:

I think a thin layer won't hurt things, good luck.


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Offlinepsilocybespawn
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: sci33]
    #2372210 - 02/23/04 03:44 PM (15 years, 4 months ago)

Very nice grow log! Excellent work. As far as the overlay goes, I think you may need to increase the temperature in your terrarium. To my experience with Pans, and I have some, the fruiting temps need to be at least at or above 76F to achieve a good flush. Just my two cents...


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OfflineLawrence
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: sci33]
    #2373310 - 02/23/04 09:10 PM (15 years, 4 months ago)

Hooo P.Cyanescens Hawaii Coral Kingdom look very potent in Psilocibin !


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Offlinecomario2
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: sci33]
    #2375152 - 02/24/04 10:24 AM (15 years, 4 months ago)

so, how are they coming along? :smile:


--------------------
comario


"crusaders against emotional poverty"


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OfflinesinoptiK
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: comario2]
    #2375856 - 02/25/04 01:05 PM (15 years, 4 months ago)

give us an update sci33 :laugh:


Edited by sinoptiK (02/25/04 01:05 PM)


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Invisiblesci33
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: sinoptiK]
    #2376277 - 02/25/04 03:54 PM (15 years, 4 months ago)

I haven't posted an update because there are still no pins, but per request I just took some pictures for you. Teonan ended up being correct, when I gave in and cased them with another thin layer, they continued vegetative growth and shot through it, delaying the pinset. Lesson learned with pans, for next time unless the overlay looks really bad, just let it be.

Isolate 1:



Isolate 2:



Close up of some growth:



In the meantime while all this has been going on, isolate 3 finished incubating in the jars. The colonized substrate was layed out in a different tray and cased last night. Once the mycelium breaks through the casing I will post pictures of this as well - this time I will not case it more than once and see what happens. The trays in the above pictures are about 3" deep with substrate, and have a footprint of 5"x5". Through my reading, I have been told that Panaeolus is more efficient with more surface area and less substrate depth. So beginning with isolate 3, I am using different sized trays which are only 2" deep and have a footprint of around 4.5"x7". With the old trays I was only able to fit 6 in my fruiting chamber, with my new trays I can fit 8. Also, some RGS jars are incubating with a multispore innoculation, when room frees up in the fruiting chamber, I will move them in and compare a multispore tray to the isolates, as well as cloning the best fruits on agar and posting pics of that process. Finally, with regards to the air exchange, a cool mist humidifier was set on a timer of 30 minutes on, 60 minutes off. Since the cool mist humidfier puts out low humidity air and is mainly for air exchange, I felt the 60 minutes of 'off' time was not enough for the humidity to build up again. So today I changed it to 30 minutes on, 90 minutes off.

Three other strains have been germinated on agar, Jamaican, Hawaiian, and Tai-KS. I will work with them on agar first, because anyway I have run out of room in the fruiting chamber. Information about that will be posted in another thread when I'm ready.

I love you all.

-sci


--------------------
Looking for:
Ps. weilii, Ps. samuiensis, Ps. hispanica, Pan. cyanescens (any strain I don't have)

Have:
Ps. cubensis, Ps. tampanensis, Pan. cyanescens (Australian, Tai-KS, Hawaiian, Jamaican, Ban Lipa Noi)

PM for a trade.


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Invisiblesci33
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: sci33]
    #2377105 - 02/25/04 09:56 PM (15 years, 4 months ago)

OK! I just did a temperature check and it turns out my fruiting chamber was warmer that I thought. It was around 85-87 degrees in there. I'm thinking that may be why I haven't seen pins. I'm gonna set it to somewhere between 75-80. I heard pans does better at 80 degrees, but I keep cubensis in there too so I'll have to find a balance..


--------------------
Looking for:
Ps. weilii, Ps. samuiensis, Ps. hispanica, Pan. cyanescens (any strain I don't have)

Have:
Ps. cubensis, Ps. tampanensis, Pan. cyanescens (Australian, Tai-KS, Hawaiian, Jamaican, Ban Lipa Noi)

PM for a trade.


Edited by sci33 (02/25/04 10:08 PM)


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Anonymous

Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: sci33]
    #2382807 - 02/27/04 12:44 PM (15 years, 4 months ago)

Don't worry. Just wait. Any temp from 75-90 F will still produce pins. You just have to wait 5-9 days from the last time you added casing soil.


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OfflineDocPsilocybin
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: sci33]
    #2383250 - 02/27/04 02:55 PM (15 years, 4 months ago)

Looking great! Give those babies some time and air exchange and they'll work magic :wink: 

Speaking of which I've got a few casings going into pinning right now that I should grow log up.


--------------------
You can't hold a man down without staying down with him.
-- Booker T. Washington


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Offlinecomario2
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: DocPsilocybin]
    #2390249 - 03/01/04 06:30 AM (15 years, 4 months ago)

update? :smile:


--------------------
comario


"crusaders against emotional poverty"


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Offlinefruityshroom
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: sci33]
    #2390298 - 03/01/04 07:22 AM (15 years, 4 months ago)

they looked wicked. thats so awesome for a first time grow eh.

cant wait for a new update and to watch your log as they pin.
BTW where are you located worldwide?

peave! LOVE!


--------------------
"you got kids running around these days; bullet proof vests, c4, armour piecring bullets. what the fuck? its no good, they've got no skill" - benico del toro - Way of the Gun -


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Invisiblesci33
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: fruityshroom]
    #2394525 - 03/02/04 11:50 AM (15 years, 4 months ago)

Ok well they finally started pinning. Nothing spectacular yet but they're coming.. isolate 1 seems to have thicker stems than isolate 2. Will post pics later, don't have my camera with me. I also added some airstones below the wet perlite to aid in humidity and air transfer like the poor mans pod, except I also have the cool mist hooked up. My cubies haven't pinned yet though and they've been in the chamber for a long time.. but pans are always faster anyway I hear.


--------------------
Looking for:
Ps. weilii, Ps. samuiensis, Ps. hispanica, Pan. cyanescens (any strain I don't have)

Have:
Ps. cubensis, Ps. tampanensis, Pan. cyanescens (Australian, Tai-KS, Hawaiian, Jamaican, Ban Lipa Noi)

PM for a trade.


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Offlinecomario2
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: sci33]
    #2394641 - 03/02/04 12:45 PM (15 years, 4 months ago)

:thumbup: pics! :smile:


--------------------
comario


"crusaders against emotional poverty"


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Offlinedaywalker
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: sci33]
    #2395183 - 03/02/04 03:17 PM (15 years, 4 months ago)

Oooohhhh! Can't wait to see this!

:mushroom2:


--------------------
"I suggest we ... learn to love our ... selves before it ... becomes illegal."


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OfflinesinoptiK
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: daywalker]
    #2395831 - 03/02/04 05:41 PM (15 years, 4 months ago)

great news but I gotta see some pics sci :laugh:


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Anonymous

Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: sci33]
    #2396349 - 03/02/04 08:07 PM (15 years, 4 months ago)

Cambodian strain is fastest to pin. On average it usually takes 7-9 days for most strains.

To mature the buttons, once enough have formed, stop misting and increase fanning, EVAPORATION RATE. Decrease the humidity in the air. I usually leave the lid to the chamber(rubbermaid) partially open at the corners. It results in shorter stems and larger caps with better spore formation.

Congrats, and enjoy the tai-ks and hawaiians.


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Invisiblesci33
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: ]
    #2399435 - 03/03/04 06:15 PM (15 years, 4 months ago)







The containers are clear, and although I taped up the sides with duct tape, the substrate itself shrank a little bit from the sides so some pins such as the last picture are shooting up from there. I shouldn't have this problem with my new trays as they have a flat lip at the sides and nothing is exposed even if the substrate shrinks a bit. Here's a shot of isolate 3 in the new tray compared to the other tray.



--------------------
Looking for:
Ps. weilii, Ps. samuiensis, Ps. hispanica, Pan. cyanescens (any strain I don't have)

Have:
Ps. cubensis, Ps. tampanensis, Pan. cyanescens (Australian, Tai-KS, Hawaiian, Jamaican, Ban Lipa Noi)

PM for a trade.


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OfflineRebirtha
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Re: The Experiments, vol. 2: Panaeolus cyanescens Australian [Re: sci33]
    #2399444 - 03/03/04 06:17 PM (15 years, 4 months ago)

i love it. exellent work!


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Panaeolus

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