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OfflineDrakk
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Time frame for spore usage
    #23537829 - 08/13/16 12:23 PM (7 years, 5 months ago)

I had been reading alot of people asking and replying on how long spores will keep. I had gotten to the point where about the longest I had heard of was 3-3.5yrs in storage.
Well, I had approximately 5.5 yrs ago placed spores into a mason jar with distilled water and a sealed lid. About 2.5 weeks ago, I brought them out and thought what the hell....So I did the BRF method and inoculated 22 jars, not really thinking they would work. (pics to be uploaded later) but low and behold...all my jars are almost colonized and looking so nice and white and very very healthy. SO I just thought for some I would throw this info out there...BTW...the spores were from EQs I got from


Edited by Prisoner#1 (08/13/16 12:40 PM)


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Re: Time frame for spore usage [Re: Drakk]
    #23537838 - 08/13/16 12:26 PM (7 years, 5 months ago)

I would put my money on white contamination rather than cubensis mycelium.


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Re: Time frame for spore usage [Re: bodhisatta]
    #23537916 - 08/13/16 12:56 PM (7 years, 5 months ago)

I like how they look "very healthy" in the mind of someone who probably is looking at a bunch of mold. :lol:


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OfflineDrakk
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Re: Time frame for spore usage [Re: bodhisatta]
    #23537924 - 08/13/16 01:00 PM (7 years, 5 months ago)

you know, I thought of that, and have been checking quite stringently. I am extremely clean when I am doing anything with mycology. I have grown for approximately 6 yrs but took a hiatus when I had kids.

The jars look healthy and actually perfect coloring. I will take some pics and upload to this string, and you can check them out. But I believe they will be ok.


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Re: Time frame for spore usage [Re: Inocuole]
    #23537929 - 08/13/16 01:03 PM (7 years, 5 months ago)

wow....been a few years since I been on here, and I really like the ENCOURAGEMENT ......such positive responses from such knowledgeable Einsteins. thanks for such kind and uplifting words.


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Re: Time frame for spore usage [Re: Drakk]
    #23537930 - 08/13/16 01:03 PM (7 years, 5 months ago)

Either put pics or accept guesses based on probabilities.


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Re: Time frame for spore usage [Re: Inocuole]
    #23537983 - 08/13/16 01:21 PM (7 years, 5 months ago)

There's a reason you have to go into the doctors office and can't just call them up

You can always wait to author a thread until you actually have the pictures. Otherwise the advice is solid regardless of whether you would like to hear it or not


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Re: Time frame for spore usage [Re: bodhisatta]
    #23538014 - 08/13/16 01:31 PM (7 years, 5 months ago)

It's not like we haven't seen a thousand of these threads before and are just going based off of patterns. :shrug:  White =/= right and people who say their sterile technique is good always throw up red flags.


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Re: Time frame for spore usage [Re: Drakk]
    #23538037 - 08/13/16 01:40 PM (7 years, 5 months ago)

Quote:

Drakk said:
wow....been a few years since I been on here, and I really like the ENCOURAGEMENT ......such positive responses from such knowledgeable Einsteins. thanks for such kind and uplifting words.




Do you want encouragement or knowledgeable advice? We can pump your tires all day long but it won't help your grow. Or we can give you knowledge and help you do better.

In this forum if you don't have pics then no one can help you. We see 1000's of jars loaded with everything from bacteria to mold that people swear is clean. We had a guy tell off people for 20 pages of thread that his tub was fine and he was gonna love throwing it our collective faces when it flushed strong and healthy. His mycologist buddy told him we were idiots.

Then it triched out in epic fashion. We can help. But only if you let us. Your spores may well have been good. But without pics this thread is kinda pointless.

Good luck :thumbup:


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Re: Time frame for spore usage [Re: Pastywhyte]
    #23538102 - 08/13/16 02:07 PM (7 years, 5 months ago)

Yeah, I done a lot of researchin here on these forums, to see how long they are viable for... Turns out spores have been claimed viable since the shroomery began up until now... Some people claiming 13+ year old spore syringes taking off. Some of these claims have pics to prove the so called "old spores" as well. I would say if made and stored correctly, they can last indefinitely, for a decade or more.

But proof still stands, post pics or it did not happen.

:chief:


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jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."


Edited by TheChief (08/13/16 02:08 PM)


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Re: Time frame for spore usage [Re: TheChief]
    #23538122 - 08/13/16 02:14 PM (7 years, 5 months ago)

I have had spore prints I took myself end up unviable as quickly as 3 years later, and had far older ones do just fine. It's not an exact science by a long shot.


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Re: Time frame for spore usage [Re: Pastywhyte]
    #23538138 - 08/13/16 02:22 PM (7 years, 5 months ago)

I think the storage method plays a big role. Over a short period of time it isn't so important, but if we are talking about storing for years or more, it can be a delicate matter.

Maybe other factors play a role as well, maybe some variety of cube spores can last longer than others? :shrug:

Nobody know because there is not much factual information backing these claims. Cubes are a controlled substance, and for that reason, these things are not documented very well. Everything is hear say, we are not professional scientists who document every detail with factual proof backing them.

Personally, I have gotten syringes almost 2 years old going. These were stored in the fridge around 1C. One thing I noted though, it took mutiple plates to get these going... Rather than one plate it usually takes for fresh spores. This means that there were a lot of unviable spores in the old syringes, it also makes me come to the conclusion that some spores of the same variety will last longer than others. Stronger genetics of single spores may be playing a role here, I don't know.

:chief:


Edited by TheChief (08/13/16 08:30 PM)


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OfflineDrakk
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Re: Time frame for spore usage [Re: TheChief]
    #23538166 - 08/13/16 02:36 PM (7 years, 5 months ago)

ok, number one...I wont be going off on anyone. I have grown as I said for 6 yrs...I have had my fair share of mold and contams...I recognize that you people are the pros and most everyone else compared to you is still a noob...pics are being loaded...thanks for the responses....

as far as sterile....I use a clean room which is a walkin freezer thats been converted...I usually do my shit in boxers and I have been sprayed down and cleaned to within an inch of my life....no one esle is allowed into the room...EVER...but whatever...as I said..thanks for the responses and I will take all into consideration. What I dont appreciate is the put downs or condemnations on my processes when you dont know how when or why....have a pleasant day and enjoy...


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Re: Time frame for spore usage [Re: Drakk]
    #23538175 - 08/13/16 02:42 PM (7 years, 5 months ago)

Well you haven't explained anything so far, you've just gotten offended, and have yet to post pics.  :shrug:

A clean room where you have to spray a bunch of shit down doesn't actually sound like ideal conditions for sterile work.  Are you using a SAB or LFH?


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Re: Time frame for spore usage [Re: Inocuole]
    #23538179 - 08/13/16 02:45 PM (7 years, 5 months ago)

The room that I do everything in is far from clean. I have success because I use good techniques and recognize where the contam vectors lay. Although it does sound like a lot of 20 year old knowledge being employed so far, I am going to reserve judgement until I see pics.


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Re: Time frame for spore usage [Re: Pastywhyte]
    #23538185 - 08/13/16 02:47 PM (7 years, 5 months ago)

Quote:

Pastywhyte said:
The room that I do everything in is far from clean. I have success because I use good techniques and recognize where the contam vectors lay. Although it does sound like a lot of 20 year old knowledge being employed so far, I am going to reserve judgement until I see pics.



:whathesaid:

You don't need a clean room. All you need is still air or a flow hood.

:chief:


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jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."


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OfflineDrakk
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Re: Time frame for spore usage [Re: Drakk]
    #23538191 - 08/13/16 02:51 PM (7 years, 5 months ago)


now I am not a pro by any stretch, but they jars I have all look like this, some colonized quicker as usually happens, but all looks good and as I stated..healthy..

also...little info....been growing oyster mushrooms for about 2 years now off of spores from a corporate growing company on the island here and as long as stored in a constant temp, in fluid of clear and clean water they can stay viable for years....but thats just me doing research and studying before I ever try to grow anything.

have a great day all, and again, thanks for the input...I shall use it as best as I see fit.


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Re: Time frame for spore usage [Re: Drakk]
    #23538246 - 08/13/16 03:15 PM (7 years, 5 months ago)

While it does look like cubes I'm not liking the look of the tabs in those jars nor the growth pattern. Definitely bacterial. However provided they are fruited as cakes and not spawned or providing the fruiting chamber is decent (like a SGFC) there is no reason those won't put out a flush.


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Re: Time frame for spore usage [Re: Pastywhyte]
    #23538298 - 08/13/16 03:40 PM (7 years, 5 months ago)

Quote:

Pastywhyte said:
I have had spore prints I took myself end up unviable as quickly as 3 years later, and had far older ones do just fine. It's not an exact science by a long shot.





Yeah I swiped a bunch of old prints to agar a few weeks ago. PE and GT printed in 2012. Zero growth on those plates. No bacteria, no molds. etc. Just nothin.

I think slants are the only way to go. I mean I guess you could just do the PP5 containers in a monotub with a bunch of diff varieties each year or two to keep your print selection going. :shrug:


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Re: Time frame for spore usage [Re: d0urd3n]
    #23539534 - 08/14/16 01:10 AM (7 years, 5 months ago)

Quote:

Pastywhyte said:
While it does look like cubes I'm not liking the look of the tabs in those jars nor the growth pattern. Definitely bacterial.




This made me take a much harder look at the pic.

That is a very good eye you have there sir.

I have an eight year old print from a wild cub that was found in Oklahoma that another user sent me. It is still in the envelope it was delivered in, unopened.

Damn how time flies when you're having fun.
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OfflineDrakk
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Re: Time frame for spore usage [Re: d0urd3n]
    #23540982 - 08/14/16 02:05 PM (7 years, 5 months ago)

north of it is just perlite border for contams. I always usually place about 1in to the top. clean edge with lysol wipes and a spray...
as I said...I know everyone has preferences to cleanliness, but I really am a clean freak....If I ever see any discolor, I throw out the whole jar....some guys try to save, I figure better safe than sorry later.

thanks for the input tho...cheers


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Re: Time frame for spore usage [Re: Drakk]
    #23540993 - 08/14/16 02:08 PM (7 years, 5 months ago)

Perlite is not a filter material. We use a dry vermiculite layer for that and we ensure it stays dry by removing the foil after inoculation.


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Re: Time frame for spore usage [Re: Pastywhyte]
    #23541043 - 08/14/16 02:21 PM (7 years, 5 months ago)

This is absolutely the first I'm ever hearing of perlite being used as a filter.


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Re: Time frame for spore usage [Re: Inocuole]
    #23552944 - 08/18/16 10:59 AM (7 years, 5 months ago)

sorry sorry sorry....it was verm I used....I had to go back once I said it and check my recipe and the product used...I am growing oyster shrroms as well,,,and it uses perlite....so my bad on the post...


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Re: Time frame for spore usage [Re: Drakk]
    #23553187 - 08/18/16 12:17 PM (7 years, 5 months ago)

I have read some where that RR (i guess) revived a 10 years old print.


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Re: Time frame for spore usage [Re: oontribe]
    #23553254 - 08/18/16 12:51 PM (7 years, 5 months ago)

Quote:

oontribe said:
I have read some where that RR (i guess) revived a 10 years old print.




Yep. He used lab grade rattlesnake venom to pull it off. Requires a background check and costs 800 bux per gram or something like that.


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Re: Time frame for spore usage [Re: Inocuole]
    #23553472 - 08/18/16 02:25 PM (7 years, 5 months ago)

Quote:

Inocuole said:
This is absolutely the first I'm ever hearing of perlite being used as a filter.



I know the guy made a typo but it is used as a filter in industry.

wiki reckons 8% of perlite is used as a "filter aid"
https://en.wikipedia.org/wiki/Perlite#Production_and_uses

I have wondered if it could be used as a filter on jars, for people who find it difficult to source stuff. I have used silicone hose as filters on jars, stuffing them with sponges. You could have sponge at the bottom and then perilite on top of that, the sponge holding it, possibly with more sponge on top to contain it. If crushed up it would be more compact, could be a use for the dust at the end of a bag.

Most people growing PF cakes will also have perlite, but some people growing on grains may have no need for verm, but may have perlite.

I doubt the verm or perlite will have the micron rating of recommended fitler material. But if it is thick enough it should do the job, in another thread I was saying if you had a sieve with 1mm holes then lots of 0.9mm diameter polystyrene balls would fall through. If you had 50 sieves on top of each other I expect few would make it to the bottom. My silicone tube sponge filters would have 1-2 inches of sponge in them.


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Re: Time frame for spore usage [Re: blackout]
    #23553617 - 08/18/16 03:04 PM (7 years, 5 months ago)

Would hydrating old spores potentially help them germinate on agar?  I have a Tamp print that I'm assuming is a few years old and for the life of me I can't get anything to grow from it.


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Re: Time frame for spore usage [Re: MountainManDan]
    #23608185 - 09/03/16 02:21 PM (7 years, 4 months ago)

so, dejarred yesterday, beautiful smell fruity I like to say....the spores colonized the jars nicely. took out yesterday and dunked for 24 hrs...placed in tubs today and everything looks healthy and nice. Will take a pic daily to upload in case I am completely retarded...

I understand some people reluctance to say what a time frame for spores is, so I will see....after 5 yrs I was kinda on the back burner as well, but hey what have I got to lose...if they grow awesome...if not..oh well...not gonna cry bout it, will just order new spores and try again...

BUT if they do work, then the way I had them stored would work for anyone..

will upload pics tomorrow morn, and every morn until I see growth..

Cheers
W


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Re: Time frame for spore usage [Re: Drakk]
    #23608270 - 09/03/16 02:43 PM (7 years, 4 months ago)

A fruity smell is not a good thing btw.


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Re: Time frame for spore usage [Re: Mushierage]
    #23608315 - 09/03/16 02:57 PM (7 years, 4 months ago)

fruity like a 'fruit:mushroom2:' or fruity like a jug of juice left in the sun? :puke:


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Re: Time frame for spore usage [Re: mushboy]
    #23608399 - 09/03/16 03:20 PM (7 years, 4 months ago)

Sweet smells are bacteria.  Just clean myc smells kinda musty, not particularly sweet though.


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Re: Time frame for spore usage [Re: Drakk]
    #23611481 - 09/04/16 02:00 PM (7 years, 4 months ago)

Quote:

Drakk said:
so, dejarred yesterday, beautiful smell fruity I like to say....the spores colonized the jars nicely. took out yesterday and dunked for 24 hrs...placed in tubs today and everything looks healthy and nice. Will take a pic daily to upload in case I am completely retarded...

I understand some people reluctance to say what a time frame for spores is, so I will see....after 5 yrs I was kinda on the back burner as well, but hey what have I got to lose...if they grow awesome...if not..oh well...not gonna cry bout it, will just order new spores and try again...

BUT if they do work, then the way I had them stored would work for anyone..

will upload pics tomorrow morn, and every morn until I see growth..

Cheers
W




Fruity/mushroomy is good, fruity/sweet is bad. 

It can be hard to describe smells properly sometimes, however once you smell the good and very distinct smell of healthy mycelium it becomes easy to recognize.

I have used old prints as well as old syringes with success. Properly made prints and/or syringes remain viable for a long time. They might not be ideal past a certain point, but I'm not sure anyone can really say definitively when that point pivots.

Also hope you gave them a nice roll in vermiculite after your dunk.

Good luck on your grow and post back with those pics!

:costanza:


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Re: Time frame for spore usage [Re: george castanza]
    #23650798 - 09/16/16 03:34 PM (7 years, 4 months ago)

ok, so after speaking with you guys and gals, I was unsure, but thought what the hell....So here are a few shots of the babies growing in, clean and no contams....



lemme know what you think...they look fantastic and are growing great

Cheers


FIVE YEARS IN STORAGE...AND STILL VIABLE


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Re: Time frame for spore usage [Re: Drakk]
    #23650835 - 09/16/16 03:55 PM (7 years, 4 months ago)

Cut the foil down to the circumference of the cake. In a 0 shape rather than a o shape. I love BRF cakes. Never ceases to amaze me those little cock suckers!


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Re: Time frame for spore usage [Re: JacobStorm]
    #23656935 - 09/18/16 08:29 PM (7 years, 4 months ago)

ok, I will do it, but could you explain the reason. I love how they are growing nice and clean. very nice, almost every cake has growth on them except like 4 out of 22 cakes..the mycelium is however continuing to grow and look so strong.


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InvisibleInocuole
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Re: Time frame for spore usage [Re: Drakk]
    #23656941 - 09/18/16 08:32 PM (7 years, 4 months ago)

Because perlite can't evaporate water with foil covering the top of it, nor can air flow up through the perlite at all.  Big flat surfaces ruin air flow in a box with holes.


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OfflineMushierage
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Re: Time frame for spore usage [Re: Drakk]
    #23658032 - 09/19/16 07:43 AM (7 years, 4 months ago)

Yap.  That's why you'll see a lot of people just use jar lids as opposed to using foil. 

Unless you need those jar lids to keep colonizing new stuff.


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InvisibleJacobStorm
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Re: Time frame for spore usage [Re: Mushierage]
    #23658844 - 09/19/16 01:44 PM (7 years, 4 months ago)

Quote:

Mushierage said:
Yap.  That's why you'll see a lot of people just use jar lids as opposed to using foil. 

Unless you need those jar lids to keep colonizing new stuff.




My jar lids started to rust.


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