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Drakk
Drakk Lord


Registered: 08/08/03
Posts: 35
Loc: Van. ISland, BC
Last seen: 7 years, 4 months
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Time frame for spore usage
#23537829 - 08/13/16 12:23 PM (7 years, 5 months ago) |
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I had been reading alot of people asking and replying on how long spores will keep. I had gotten to the point where about the longest I had heard of was 3-3.5yrs in storage. Well, I had approximately 5.5 yrs ago placed spores into a mason jar with distilled water and a sealed lid. About 2.5 weeks ago, I brought them out and thought what the hell....So I did the BRF method and inoculated 22 jars, not really thinking they would work. (pics to be uploaded later) but low and behold...all my jars are almost colonized and looking so nice and white and very very healthy. SO I just thought for some I would throw this info out there...BTW...the spores were from EQs I got from
Edited by Prisoner#1 (08/13/16 12:40 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Time frame for spore usage [Re: Drakk]
#23537838 - 08/13/16 12:26 PM (7 years, 5 months ago) |
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I would put my money on white contamination rather than cubensis mycelium.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Time frame for spore usage [Re: bodhisatta]
#23537916 - 08/13/16 12:56 PM (7 years, 5 months ago) |
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I like how they look "very healthy" in the mind of someone who probably is looking at a bunch of mold.
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Drakk
Drakk Lord


Registered: 08/08/03
Posts: 35
Loc: Van. ISland, BC
Last seen: 7 years, 4 months
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Re: Time frame for spore usage [Re: bodhisatta]
#23537924 - 08/13/16 01:00 PM (7 years, 5 months ago) |
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you know, I thought of that, and have been checking quite stringently. I am extremely clean when I am doing anything with mycology. I have grown for approximately 6 yrs but took a hiatus when I had kids.
The jars look healthy and actually perfect coloring. I will take some pics and upload to this string, and you can check them out. But I believe they will be ok.
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Drakk
Drakk Lord


Registered: 08/08/03
Posts: 35
Loc: Van. ISland, BC
Last seen: 7 years, 4 months
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Re: Time frame for spore usage [Re: Inocuole]
#23537929 - 08/13/16 01:03 PM (7 years, 5 months ago) |
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wow....been a few years since I been on here, and I really like the ENCOURAGEMENT ......such positive responses from such knowledgeable Einsteins. thanks for such kind and uplifting words.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Time frame for spore usage [Re: Drakk]
#23537930 - 08/13/16 01:03 PM (7 years, 5 months ago) |
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Either put pics or accept guesses based on probabilities.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Time frame for spore usage [Re: Inocuole]
#23537983 - 08/13/16 01:21 PM (7 years, 5 months ago) |
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There's a reason you have to go into the doctors office and can't just call them up
You can always wait to author a thread until you actually have the pictures. Otherwise the advice is solid regardless of whether you would like to hear it or not
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Time frame for spore usage [Re: bodhisatta]
#23538014 - 08/13/16 01:31 PM (7 years, 5 months ago) |
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It's not like we haven't seen a thousand of these threads before and are just going based off of patterns. White =/= right and people who say their sterile technique is good always throw up red flags.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Time frame for spore usage [Re: Drakk]
#23538037 - 08/13/16 01:40 PM (7 years, 5 months ago) |
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Quote:
Drakk said: wow....been a few years since I been on here, and I really like the ENCOURAGEMENT ......such positive responses from such knowledgeable Einsteins. thanks for such kind and uplifting words.
Do you want encouragement or knowledgeable advice? We can pump your tires all day long but it won't help your grow. Or we can give you knowledge and help you do better.
In this forum if you don't have pics then no one can help you. We see 1000's of jars loaded with everything from bacteria to mold that people swear is clean. We had a guy tell off people for 20 pages of thread that his tub was fine and he was gonna love throwing it our collective faces when it flushed strong and healthy. His mycologist buddy told him we were idiots.
Then it triched out in epic fashion. We can help. But only if you let us. Your spores may well have been good. But without pics this thread is kinda pointless.
Good luck
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TheChief
Cube Collector



Registered: 07/09/14
Posts: 1,905
Loc: Executing TEKs...
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Re: Time frame for spore usage [Re: Pastywhyte]
#23538102 - 08/13/16 02:07 PM (7 years, 5 months ago) |
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Yeah, I done a lot of researchin here on these forums, to see how long they are viable for... Turns out spores have been claimed viable since the shroomery began up until now... Some people claiming 13+ year old spore syringes taking off. Some of these claims have pics to prove the so called "old spores" as well. I would say if made and stored correctly, they can last indefinitely, for a decade or more.
But proof still stands, post pics or it did not happen.
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jcbowling1985 said: "Additionally I did one Armageddon jar with all of the types together."
Edited by TheChief (08/13/16 02:08 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Time frame for spore usage [Re: TheChief]
#23538122 - 08/13/16 02:14 PM (7 years, 5 months ago) |
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I have had spore prints I took myself end up unviable as quickly as 3 years later, and had far older ones do just fine. It's not an exact science by a long shot.
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TheChief
Cube Collector



Registered: 07/09/14
Posts: 1,905
Loc: Executing TEKs...
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Re: Time frame for spore usage [Re: Pastywhyte]
#23538138 - 08/13/16 02:22 PM (7 years, 5 months ago) |
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I think the storage method plays a big role. Over a short period of time it isn't so important, but if we are talking about storing for years or more, it can be a delicate matter.
Maybe other factors play a role as well, maybe some variety of cube spores can last longer than others?
Nobody know because there is not much factual information backing these claims. Cubes are a controlled substance, and for that reason, these things are not documented very well. Everything is hear say, we are not professional scientists who document every detail with factual proof backing them.
Personally, I have gotten syringes almost 2 years old going. These were stored in the fridge around 1C. One thing I noted though, it took mutiple plates to get these going... Rather than one plate it usually takes for fresh spores. This means that there were a lot of unviable spores in the old syringes, it also makes me come to the conclusion that some spores of the same variety will last longer than others. Stronger genetics of single spores may be playing a role here, I don't know.
Edited by TheChief (08/13/16 08:30 PM)
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Drakk
Drakk Lord


Registered: 08/08/03
Posts: 35
Loc: Van. ISland, BC
Last seen: 7 years, 4 months
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Re: Time frame for spore usage [Re: TheChief]
#23538166 - 08/13/16 02:36 PM (7 years, 5 months ago) |
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ok, number one...I wont be going off on anyone. I have grown as I said for 6 yrs...I have had my fair share of mold and contams...I recognize that you people are the pros and most everyone else compared to you is still a noob...pics are being loaded...thanks for the responses....
as far as sterile....I use a clean room which is a walkin freezer thats been converted...I usually do my shit in boxers and I have been sprayed down and cleaned to within an inch of my life....no one esle is allowed into the room...EVER...but whatever...as I said..thanks for the responses and I will take all into consideration. What I dont appreciate is the put downs or condemnations on my processes when you dont know how when or why....have a pleasant day and enjoy...
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Time frame for spore usage [Re: Drakk]
#23538175 - 08/13/16 02:42 PM (7 years, 5 months ago) |
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Well you haven't explained anything so far, you've just gotten offended, and have yet to post pics. 
A clean room where you have to spray a bunch of shit down doesn't actually sound like ideal conditions for sterile work. Are you using a SAB or LFH?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Time frame for spore usage [Re: Inocuole]
#23538179 - 08/13/16 02:45 PM (7 years, 5 months ago) |
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The room that I do everything in is far from clean. I have success because I use good techniques and recognize where the contam vectors lay. Although it does sound like a lot of 20 year old knowledge being employed so far, I am going to reserve judgement until I see pics.
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TheChief
Cube Collector



Registered: 07/09/14
Posts: 1,905
Loc: Executing TEKs...
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Re: Time frame for spore usage [Re: Pastywhyte]
#23538185 - 08/13/16 02:47 PM (7 years, 5 months ago) |
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Quote:
Pastywhyte said: The room that I do everything in is far from clean. I have success because I use good techniques and recognize where the contam vectors lay. Although it does sound like a lot of 20 year old knowledge being employed so far, I am going to reserve judgement until I see pics.
You don't need a clean room. All you need is still air or a flow hood.
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jcbowling1985 said: "Additionally I did one Armageddon jar with all of the types together."
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Drakk
Drakk Lord


Registered: 08/08/03
Posts: 35
Loc: Van. ISland, BC
Last seen: 7 years, 4 months
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Re: Time frame for spore usage [Re: Drakk]
#23538191 - 08/13/16 02:51 PM (7 years, 5 months ago) |
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 now I am not a pro by any stretch, but they jars I have all look like this, some colonized quicker as usually happens, but all looks good and as I stated..healthy..
also...little info....been growing oyster mushrooms for about 2 years now off of spores from a corporate growing company on the island here and as long as stored in a constant temp, in fluid of clear and clean water they can stay viable for years....but thats just me doing research and studying before I ever try to grow anything.
have a great day all, and again, thanks for the input...I shall use it as best as I see fit.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Time frame for spore usage [Re: Drakk]
#23538246 - 08/13/16 03:15 PM (7 years, 5 months ago) |
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While it does look like cubes I'm not liking the look of the tabs in those jars nor the growth pattern. Definitely bacterial. However provided they are fruited as cakes and not spawned or providing the fruiting chamber is decent (like a SGFC) there is no reason those won't put out a flush.
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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Re: Time frame for spore usage [Re: Pastywhyte]
#23538298 - 08/13/16 03:40 PM (7 years, 5 months ago) |
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Quote:
Pastywhyte said: I have had spore prints I took myself end up unviable as quickly as 3 years later, and had far older ones do just fine. It's not an exact science by a long shot.
Yeah I swiped a bunch of old prints to agar a few weeks ago. PE and GT printed in 2012. Zero growth on those plates. No bacteria, no molds. etc. Just nothin.
I think slants are the only way to go. I mean I guess you could just do the PP5 containers in a monotub with a bunch of diff varieties each year or two to keep your print selection going.
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george castanza
Lord Of The Idiots!


Registered: 10/21/02
Posts: 8,723
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Re: Time frame for spore usage [Re: d0urd3n]
#23539534 - 08/14/16 01:10 AM (7 years, 5 months ago) |
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Quote:
Pastywhyte said: While it does look like cubes I'm not liking the look of the tabs in those jars nor the growth pattern. Definitely bacterial.
This made me take a much harder look at the pic.
That is a very good eye you have there sir.
I have an eight year old print from a wild cub that was found in Oklahoma that another user sent me. It is still in the envelope it was delivered in, unopened.
Damn how time flies when you're having fun.
 
-------------------- KRAMER CAKES


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