Home | Community | Message Board

Sporeworks
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   Myyco.com Isolated Cubensis Liquid Culture For Sale   Bridgetown Botanicals CBD Concentrates   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinemycoic
Stranger
Registered: 07/02/16
Posts: 51
Last seen: 7 years, 2 months
Green Mold/Trich/ASP In Rye Grain/Wheat Aswell..
    #23536577 - 08/12/16 11:21 PM (7 years, 6 months ago)

Good Afternoon Everyone,

SO here's the story:

*All performed in a SAB with shit loads of bleached used, Hepa filter (store bought one) scrubbed the air for 24 hours before all events (switched off 1 hour prior)
Cleaned area and floors using disinfectant and use a drop sheet with bleach/alcohol on the table.
-Wearing a tyvek painting suit with a surgical mask also.
-SFD's on all jars used.
-Gloves worn and everything in the room cleaned and sanitized.

- 1# Used Spore Print from a vendor and did this method for syringes:

-PC'd a few jars for 45min @ 15psi.
-Had fresh Sterile Syringes and needles (in package)
-Fresh Sterile inj water from chemist.
-Innoc loop and blow torch for the scraping and heating.

-opened jar in SAB, poured in water from the container and then flamed needle and sucked up spores.

-Resulted in green mold/trich in what grain spawn bags and rye grain spawn bags. Bags had SHIP's also.

#2 Figured i cocked that one up.. So fresh spores from same vendor -  but newer.
Again...

-PC'd a few jars for 45min @ 15psi.
-Had fresh Sterile Syringes and needles (in package)
-Fresh Sterile inj water from chemist.
-Innoc loop and blow torch for the scraping and heating.

-Used jars this time.
-Did a Agar wedge to 1 jar, innoc'd 2 using fresh syringes made (again out of packets..)
-Jar 1 Agar to Grain, Jar 2 Syringe #1 (3cc Syringe), Jar 2 Syringe #1, Spawn bag of wheat grain Syringe # 2
-Rye started colonizing in all 3 jars and bag.
- All Got to 30% in roughly 1.5 weeks. Shook Jar 1 (Agar) no mold, clean nice myc growth.
- Jar 2 got to 30%, shook this morning and all was good before shaking, shook and a giant clump had green mold on it. Removed jar. Havent shaken Jar 3 yet.. Spawn bag looks fine so far with no mold appreaing and fresh myc on bottom.

Only thing i'm not doing is boiling water and letting syringes cool. The syringes have been in sealed packets..and needles.

Flame for needle is as follows.. Flame needle till red hot, squirt water, let cool, re flame and wait then innoc.

What am i doing wrong? the Agar to grain worked perfect and i've done open air innoc with spawn bags using SHIP's all worked perfect.

Real confused.. Is sterile syringes in packets really sterile or should I boil water and do that method.

I'm thinking for this new attempt i'm going to leave sealed syringes in foil, put in quart jars and pc them also.. Desperate.

Thinking it's Asp, not trich. see exact same looking pic below.

https://files.shroomery.org/files/12-50/519362875-3.jpg

Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,819
Loc: Canada Flag
Trusted Cultivator
Re: Green Mold/Trich/ASP In Rye Grain/Wheat Aswell.. [Re: mycoic]
    #23537380 - 08/13/16 09:27 AM (7 years, 6 months ago)

How are you prepping your grain? What kind of filters are you using? Pics of everything will tell more than your words could.

The best way to troubleshoot is to show us a pic and let us ask you questions. You typing a wall of text about the things you think are important for us to know is not going to be effective.

Extras: Filter Print Post Top
Offlinemycoic
Stranger
Registered: 07/02/16
Posts: 51
Last seen: 7 years, 2 months
Re: Green Mold/Trich/ASP In Rye Grain/Wheat Aswell.. [Re: Pastywhyte]
    #23537401 - 08/13/16 09:40 AM (7 years, 6 months ago)

Thanks for the reply,

With the Jars I've done exactly like RR has done using SFD's. Not cut or anything just holes in the lid with the SFD on top and then the ring to secure.

So far its been 1 Jar, 2x Wheat Grain baths and 1x Rye grain bags that had the same contam.

Also is at the top of jar, where as the bags it was everywhere and more localised around the innoc point.

I've binned the bags, but the jar was discovered this morning so i'll get photos soon.

Weird how the Agar to grain was okay and all agar has been fine, including multiple transfers.

Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,819
Loc: Canada Flag
Trusted Cultivator
Re: Green Mold/Trich/ASP In Rye Grain/Wheat Aswell.. [Re: mycoic]
    #23537425 - 08/13/16 09:49 AM (7 years, 6 months ago)

Maybe your not transferring what you think you are. Molds like trich have white mycelium and will mesh in with the mushroom mycelium becoming difficult to spot to the untrained eye. There are certain growth patterns that we need to look for. White and fuzzy is often not enough to be sure.

Extras: Filter Print Post Top
Offlinemycoic
Stranger
Registered: 07/02/16
Posts: 51
Last seen: 7 years, 2 months
Re: Green Mold/Trich/ASP In Rye Grain/Wheat Aswell.. [Re: Pastywhyte]
    #23539761 - 08/14/16 03:16 AM (7 years, 6 months ago)

Cheers for the reply, I actually used your tek for the agar too but with 1/4 quart jars haha.

So, I was thinking over my procedures for everything and couldn't work out if i was missing something; I started looking through RR vid's etc too see if i did, brushed up on my SAB procedures and whilst doing so realised why the issue was..

The last batch I more than likely botched due to my inner loop not getting red hot, but these syringes were done much better..

The Jar was contam'd right at top and in the middle, but the others weren't and the Agar seems okay atm, checked top of jar and I have a SHIP under the SFD which is raising it and not sealing correctly..

Extras: Filter Print Post Top
Offlinegone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 6 months, 18 days
Re: Green Mold/Trich/ASP In Rye Grain/Wheat Aswell.. [Re: mycoic] * 1
    #27373587 - 07/04/21 05:14 AM (2 years, 7 months ago)

Agar to grain is much more reliable than spores to grain. Your spore suppliers aren't generally clean enough to give you a contam-free spore print/syringe. And 45 minutes is considered too short: 90 minutes is touted as the minimum, while 2 hours is the standard recommendation. And that's after venting steam for ten minutes to increase the partial pressure of water in the headspace.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Kraken Kratom Red Vein Kratom   Myyco.com Isolated Cubensis Liquid Culture For Sale   Bridgetown Botanicals CBD Concentrates   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* The ease of rye grain. Anonymous 14,175 19 06/03/16 03:10 PM
by Disco Shiny
* beating green mold. Shooms still good? PIC abrcromby_dude 5,328 13 02/20/14 11:43 PM
by Happy Littletree
* growin zoomies with green mold next door abrcromby_dude 1,292 4 12/03/02 11:45 PM
by abrcromby_dude
* shrooms growin, but green mold in corner. abrcromby_dude 2,299 1 12/04/02 12:43 AM
by WildCardsRevenge
* Re: Rye grain - Always a bitch?
( 1 2 all )
vts1134 4,355 23 03/27/01 10:03 AM
by Ryche Hawk
* 90% green molds are NOT Trichoderma. Zen Peddler 7,562 5 04/02/02 01:39 PM
by Seuss
* It is possible to salvage jars 50% contamed by Green Mold CultyVader 4,274 6 05/18/03 12:59 AM
by CultyVader
* rye grain problem robinhood2 3,139 14 08/31/02 09:22 AM
by indkebr

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,189 topic views. 26 members, 162 guests and 29 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.01 seconds on 14 queries.