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OfflinePieEyed-Messiah
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Registered: 11/24/15
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Using mycelium
    #23537300 - 08/13/16 08:39 AM (7 years, 5 months ago)

So I had a huge issue and lost almost everything I have a qt jar with mycelium in it nice white clumps the jar was dumped out there is also trich in the jar I have no spores or cultures left how do I salvage this mycelium? Please help


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Invisibleweetsie
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Re: Using mycelium [Re: PieEyed-Messiah]
    #23537314 - 08/13/16 08:49 AM (7 years, 5 months ago)

Take really small pieces of mycelium and drop them on agar and go from there.


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Offlinelukehighwalker710
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Re: Using mycelium [Re: PieEyed-Messiah]
    #23537317 - 08/13/16 08:50 AM (7 years, 5 months ago)

It's going to be a little work to clean it up but if that's really all you have then you better do it right.

Agar is the answer. I'd start by reading into Pasty's agar tek. You can transfer a grain to some plates in a still air box (SAB) and as soon as you get healthy growth transfer to a new plate, away from contams. Repeat as necessary.

Some cases may necessitate the use of the agar sandwich isolation tek. Its going to take some good timing, and steady sterile work but all hope is not lost.



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Edited by lukehighwalker710 (08/13/16 08:51 AM)


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
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Re: Using mycelium [Re: lukehighwalker710]
    #23537323 - 08/13/16 08:54 AM (7 years, 5 months ago)

.


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Edited by amidogen (06/21/18 06:50 AM)


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InvisiblebodhisattaMDiscordReddit
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Re: Using mycelium [Re: amidogen]
    #23537649 - 08/13/16 11:26 AM (7 years, 5 months ago)

I wouldn't bother to be honest. Chances are you're not saving it


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OfflinePieEyed-Messiah
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Re: Using mycelium [Re: bodhisatta]
    #23537755 - 08/13/16 11:55 AM (7 years, 5 months ago)

Yea I was seriously considering just buying new spore syringes


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OfflinePieEyed-Messiah
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Re: Using mycelium [Re: PieEyed-Messiah]
    #23537762 - 08/13/16 11:56 AM (7 years, 5 months ago)

I have some blue/pink oysters and some reshi cultures to play with until I get my new batch


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InvisiblebodhisattaMDiscordReddit
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Re: Using mycelium [Re: PieEyed-Messiah]
    #23537768 - 08/13/16 11:58 AM (7 years, 5 months ago)

You can try but it will be such an uphill battle that 10$ is super duper worth it


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OfflinePieEyed-Messiah
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Re: Using mycelium [Re: PieEyed-Messiah]
    #23537774 - 08/13/16 11:59 AM (7 years, 5 months ago)

Although if I do get it to work or just the process I feel might teach me alot I have trouble cloning I always get contamination so this might teach me trial by fire on my steril process


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InvisiblebodhisattaMDiscordReddit
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Re: Using mycelium [Re: PieEyed-Messiah]
    #23537795 - 08/13/16 12:03 PM (7 years, 5 months ago)

In the spare time yea it's not a bad idea to try things. That's how you turn into an expert


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InvisibletheGODSmademedoit

Registered: 03/04/16
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Re: Using mycelium [Re: bodhisatta]
    #23537901 - 08/13/16 12:51 PM (7 years, 5 months ago)

Yes get in to agar and you wont run into the problem of no myc again


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