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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 11 months, 13 days
n00b grow log
    #23536642 - 08/12/16 11:50 PM (7 years, 6 months ago)

Hey guys been lurking here for awhile and thought I'd post my second grow attempt ever to get some feedback. My first attempt was a damn mess. Long story short it ended in two quart sized BRF cakes in a SGFC and pushed out a zip or two.
 
  Right now the goal is four mini monos for each variety I've got: B+, Penis Envy, Golden Teacher, and Puerto Rican. I'll be including links to teks I use in the process since I did a lot of my learning from searching terms used in recent posts here. Maybe I'll help a fellow lurker out. I started with 8 pasty plates and inoculated two with each variety for no real reason other than practice.

  I didn't manage to get any good pictures of the pasty plates since they're rather hard to see through, so I opted for a different container. I did some searching and found OPB's Reusable, No Pour, Unbreakable, Glass Top, Agar Petri Dish for under $1 and thought the price was right. Here's some pics of my transfers from the pasty plates:



I only did one transfer of the B+ and two of the rest. I think I've moved them around since the first pic but in the second pic it goes from left to right B+, GT, Penis Envy, Puerto Rican. My technique could definitely use some work, I ended up with tons of inoculation points trying to unstick the damn agar chunk from the scalpel. The Puerto Ricans seem to be growing more on the Y-axis than the X. Does everything look healthy?

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InvisibleJvF
Fletcher Detcher


Registered: 02/13/14
Posts: 2,662
Loc: Chicago Flag
Re: n00b grow log [Re: Dactylium]
    #23536679 - 08/13/16 12:08 AM (7 years, 6 months ago)

Hate to say it but all your transfers have sattelite colonies, they all look contaminated.

I wouls say the one on the bottom left has a chance, so be extra careful and keep an eye on it


--------------------

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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 11 months, 13 days
Re: n00b grow log [Re: JvF]
    #23536907 - 08/13/16 02:44 AM (7 years, 6 months ago)

No worries. I'm not sure what proper colonization from multispore is supposed to look like to be honest. It's entirely possible I was transferring junk to begin with.

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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 2 months
Re: n00b grow log [Re: Dactylium]
    #23537145 - 08/13/16 06:45 AM (7 years, 6 months ago)

That rhizo culture to the middle left looks amazing.
Definitely take transfers from there, it looks as clean as a whistle.

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