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OfflineBazookatooth
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Registered: 04/09/15
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Lots of pooling water in colonized jars.
    #23535975 - 08/12/16 07:59 PM (7 years, 5 months ago)

Hello! I have a bunch of colonized jars that have a lot of water collecting at the bottom that I believe is causing the yellowing in my jars. I did some research although the answer on good to fruit are flip-floppy the reason is usually from sitting around overheating.what do to guys think? Have you guys experienced anything like this? I'll post pictures when I get home.


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Invisibleweetsie
unlicensed tub surgeon
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Registered: 05/08/11
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Re: Lots of pooling water in colonized jars. [Re: Bazookatooth]
    #23536066 - 08/12/16 08:27 PM (7 years, 5 months ago)

Lot's of discolored liquid is likely metabolites produced in response to bacteria.

You can use it but the chances of failure are higher, yields might be less that what they could be etc.

If I end up with a jar like that it goes in the garden as id rather focus my efforts on clean spawn, but circumstances are personal so by all means have a go.

EDIT: misread your post, if the liquid is clear then it's probably fine.

Why does grain sweat only when it's colonized? I have a few jars only a week since colonizing and there is an inch or two of water in the bottom of them.



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Edited by weetsie (08/12/16 09:11 PM)


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OfflinecronicrFacebook
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Re: Lots of pooling water in colonized jars. [Re: weetsie]
    #23536080 - 08/12/16 08:31 PM (7 years, 5 months ago)

my jars are doing this right now simply because they are colonized and fucking warm...post a pic


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OfflineBazookatooth
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Registered: 04/09/15
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Re: Lots of pooling water in colonized jars. [Re: cronicr]
    #23537400 - 08/13/16 09:39 AM (7 years, 5 months ago)

Sorry it took so long had a suckish night. These are the jars in question. The first two is obvious metabolites, I need to toss a tub as a result haha

Cracked open the wettest one to smell, like mushrooms, tossed it in the conpost.


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OfflineBazookatooth
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Re: Lots of pooling water in colonized jars. [Re: Bazookatooth]
    #23537413 - 08/13/16 09:45 AM (7 years, 5 months ago)

Some more pictures


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Re: Lots of pooling water in colonized jars. [Re: Bazookatooth]
    #23537455 - 08/13/16 10:01 AM (7 years, 5 months ago)

Those all look pretty bacterial. I wouldn't spawn any of that to be honest, especially the 4 in your first post. I suggest throwing them in a compost pile or topfruiting them. You could risk spawning but you gotta manage your expectations if you do that. They could fruit, bacteria often does. They are a bit too far gone to be worth the time and supplies for spawning IMO though.


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OfflineBazookatooth
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Re: Lots of pooling water in colonized jars. [Re: Supalemonhaze]
    #23537481 - 08/13/16 10:17 AM (7 years, 5 months ago)

Damn I hate tossing out hard work but I hate bad work even more.


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InvisibleSupalemonhaze
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Re: Lots of pooling water in colonized jars. [Re: Bazookatooth]
    #23537495 - 08/13/16 10:22 AM (7 years, 5 months ago)

What are you using as inoculant? And how long do you PC for?


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OfflineBazookatooth
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Re: Lots of pooling water in colonized jars. [Re: Supalemonhaze]
    #23537501 - 08/13/16 10:25 AM (7 years, 5 months ago)

Rye grain and gypsum PC for 90 minutes let cool overnight. MS syringe, grain transferred to twenty jars.


Edited by Bazookatooth (08/13/16 10:27 AM)


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Invisibleweetsie
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Re: Lots of pooling water in colonized jars. [Re: Bazookatooth]
    #23537525 - 08/13/16 10:35 AM (7 years, 5 months ago)

If you're doing that many jars I'd invest the $10 or so needed to start with agar.


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Re: Lots of pooling water in colonized jars. [Re: Bazookatooth]
    #23537529 - 08/13/16 10:37 AM (7 years, 5 months ago)

That's your problem right there I reckon. MS inoculation straight to grains is risky enough, g2ging that grains to more grains is just wreaking havoc on your spawn. Every syringe is said to contain at least a minimal amount of bacteria, which isn't too bad if you don't g2g them. With a g2g they get better chance to gain a foothold though.

Ever try agar? It's the best tool for clean spawn. I use wedges on grains and frankly, I still don't g2g because of bacteria. That's how persistent it is.


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OfflineBazookatooth
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Re: Lots of pooling water in colonized jars. [Re: Supalemonhaze]
    #23537545 - 08/13/16 10:44 AM (7 years, 5 months ago)

Thanks for the feedback to guys I really appreciate it I had no idea about g2g like that. I have some agar with some sucess but my house is ridden with contams it's so hard


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Re: Lots of pooling water in colonized jars. [Re: Bazookatooth]
    #23537546 - 08/13/16 10:45 AM (7 years, 5 months ago)

Every house in every country on every continent in all of the world has contams. It's all about sterile tek.


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OfflineBazookatooth
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Re: Lots of pooling water in colonized jars. [Re: Supalemonhaze]
    #23537562 - 08/13/16 10:51 AM (7 years, 5 months ago)

Have you dealt with mucor before? It's this shitty black sporulating mess that popped out of nowhere. Three years ago it was typical tric and cobwebbing. Then this black shit started popping up in my plates, jars and tubs. Maybe it's always been here but it will literally suffocate trich and cobweb. I seriously hope I haven't been pulling out my hair for the better part of two years over my own technique lmao


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Re: Lots of pooling water in colonized jars. [Re: Bazookatooth]
    #23537575 - 08/13/16 10:56 AM (7 years, 5 months ago)

Mucor loves high temps and lack of FAE IIRC. If it's the same thing I'm thinking of (black pin mold), I had it twice on a straw laundry basket. Both times in summer.

Seriously though, if you are getting that on agar, you need to work on your sterile tek. If you are using a SAB, make sure you are moving slowly. SABs have been a pain for me so I feel you but it's worth trying to figure it out for the sake of using agar. Once you get used to agar it's almost like you magically get better overnight at growing.


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OfflineBazookatooth
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Registered: 04/09/15
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Re: Lots of pooling water in colonized jars. [Re: Supalemonhaze]
    #23537587 - 08/13/16 11:02 AM (7 years, 5 months ago)

Again thanks for all of the help, I think it's time to get back into some pastyplates.


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Offlinespacechildo
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Re: Lots of pooling water in colonized jars. [Re: Bazookatooth]
    #23537636 - 08/13/16 11:22 AM (7 years, 5 months ago)

first series of pics looks really bad, second pic post looks like they've been colonized for a long time already?


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OfflineBazookatooth
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Registered: 04/09/15
Posts: 250
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Re: Lots of pooling water in colonized jars. [Re: spacechildo]
    #23537641 - 08/13/16 11:23 AM (7 years, 5 months ago)

All have been at 100% for about two weeks, around when the water started pooling


Edited by Bazookatooth (08/13/16 11:24 AM)


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Offlinespacechildo
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Re: Lots of pooling water in colonized jars. [Re: Bazookatooth]
    #23537653 - 08/13/16 11:27 AM (7 years, 5 months ago)

why do you have jars sit 100% for weeks?


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OfflineBazookatooth
Jarl of Burger King

Registered: 04/09/15
Posts: 250
Last seen: 3 years, 6 months
Re: Lots of pooling water in colonized jars. [Re: spacechildo]
    #23537678 - 08/13/16 11:31 AM (7 years, 5 months ago)

Because I ran out of tubs to fruit and didn't know they would turn out like this. I've had jars sit around for months and eventually fruit in them, this really caught me off guard.


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