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C99
Stranger


Registered: 08/12/16
Posts: 3
Last seen: 7 years, 5 months
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Stalled growth after shaking jars.
#23535743 - 08/12/16 06:28 PM (7 years, 5 months ago) |
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I bought 8 quart rye berry spawn jars from out grow and knocked them up with 2 multi-spore syringes. After they where around 20-25% colonized I shook them. 6 out of the 8 did fine and shortly after colonized 100%. The other 2 just stopped growing. My question is why did these two jars stop growing after shaking but the other 6 didn't? Oh, it's also been about six weeks since inoculation and probably a month since shaking. Temps are around 75-80 F.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Stalled growth after shaking jars. [Re: C99]
#23535746 - 08/12/16 06:31 PM (7 years, 5 months ago) |
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Contamination is likely
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Re: Stalled growth after shaking jars. [Re: C99]
#23535757 - 08/12/16 06:34 PM (7 years, 5 months ago) |
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They almost definitely contaminated, sounds like you used a LOT of spores which are inherently dirty meaning the more you use the higher the chance of contam. Id say you did alright to get 6/8.
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
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Psykronik
OverGrower


Registered: 02/28/12
Posts: 150
Loc: Scotland
Last seen: 7 years, 4 months
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Re: Stalled growth after shaking jars. [Re: C99]
#23535760 - 08/12/16 06:35 PM (7 years, 5 months ago) |
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3 out of 4 of my first MS inoculated grain jars stalled after a shake and started looking wettish. Cracked open after a week and it smelled sweet so i put it down to bacteria. G2G the 1 that was ok. Moved to agar now and have healthy cultures. Not going to waste my time again with MS unless it's straight to agar. I would toss the jars that have stalled. Especially after being so long.
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C99
Stranger


Registered: 08/12/16
Posts: 3
Last seen: 7 years, 5 months
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Re: Stalled growth after shaking jars. [Re: Psykronik]
#23535794 - 08/12/16 06:49 PM (7 years, 5 months ago) |
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Ok thanks for the replies. I only used one syringe for 4 jars and don't visibly see any contamination, but it makes sense. Was just wandering if it might have been something else since I havn't seen any nasties in the jars. Thanks again.
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: Stalled growth after shaking jars. [Re: C99]
#23535805 - 08/12/16 06:54 PM (7 years, 5 months ago) |
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Hey well congrats on your viable jars but its a big no no about posting vendors names
But one syringe for 4 jars is a bit too much maybe 7-10 jars per 10cc syringe so stall is probably from contamination
Edited by spore-ty (08/12/16 06:56 PM)
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