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OfflineC99
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Registered: 08/12/16
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Last seen: 7 years, 5 months
Stalled growth after shaking jars.
    #23535743 - 08/12/16 06:28 PM (7 years, 5 months ago)

I bought 8 quart rye berry spawn jars from out grow and knocked them up with 2 multi-spore syringes. After they where around 20-25% colonized I shook them. 6 out of the 8 did fine and shortly after colonized 100%. The other 2 just stopped growing. My question is why did these two jars stop growing after shaking  but the other 6 didn't? Oh, it's also been about six weeks since inoculation and probably a month since shaking. Temps are around 75-80 F.


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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: Stalled growth after shaking jars. [Re: C99]
    #23535746 - 08/12/16 06:31 PM (7 years, 5 months ago)

Contamination is likely


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Invisibleweetsie
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Registered: 05/08/11
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Re: Stalled growth after shaking jars. [Re: C99]
    #23535757 - 08/12/16 06:34 PM (7 years, 5 months ago)

They almost definitely contaminated, sounds like you used a LOT of spores which are inherently dirty meaning the more you use the higher the chance of contam. Id say you did alright to get 6/8.


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OfflinePsykronik
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Registered: 02/28/12
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Re: Stalled growth after shaking jars. [Re: C99]
    #23535760 - 08/12/16 06:35 PM (7 years, 5 months ago)

3 out of 4 of my first MS inoculated grain jars stalled after a shake and started looking wettish. Cracked open after a week and it smelled sweet so i put it down to bacteria. G2G the 1 that was ok. Moved to agar now and have healthy cultures. Not going to waste my time again with MS unless it's straight to agar. I would toss the jars that have stalled. Especially after being so long.


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OfflineC99
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Registered: 08/12/16
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Last seen: 7 years, 5 months
Re: Stalled growth after shaking jars. [Re: Psykronik]
    #23535794 - 08/12/16 06:49 PM (7 years, 5 months ago)

Ok thanks for the replies.  I only used one syringe for 4 jars and don't visibly see any contamination, but it makes sense. Was just wandering if it might have been something else since I havn't seen any nasties in the jars. Thanks again.


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Offlinespore-ty
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Registered: 01/21/16
Posts: 1,028
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Re: Stalled growth after shaking jars. [Re: C99]
    #23535805 - 08/12/16 06:54 PM (7 years, 5 months ago)

Hey well congrats on your viable jars but its a big no no about posting vendors names

But one syringe for 4 jars is a bit too much maybe 7-10 jars per 10cc syringe so stall is probably from contamination


Edited by spore-ty (08/12/16 06:56 PM)


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