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SHROOM HEAD

Registered: 03/04/16
Posts: 1,933
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Re: Psilocybe subaeruginosa cultivation questions [Re: Bunya]
#23547404 - 08/16/16 04:35 PM (7 years, 5 months ago) |
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To add on to what I was saying about not isolating myc from spores from wild specimens, I just would not bother.  I would want to keep all the myc on the plate and just toss it in the substrate jars. Once you get fruit from it, then perhaps I would clone and isolate some traits.
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Bunya said: I was sort of wondering if i add some gypsum to the substrate will the sub mycelium like it or will it antagonise it.
I would not even bother doing that to be honest, but I'm not discouraging you, by all means, play around, you seem to like to play around as you are using jars with many diferent substrates, so by all means, do as you wish. 
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cronicr said: PH is not a concern as it is outdoors anyway
 Just like with the gypsum, I would not bother doing PH tests on the substrate, but by all means, you like to play around and go above and beyond and do some interesting shit, so go ahead, if you want to, that is. There is actually no point to be honest, but if you want to know then go right ahead, if you have an interest in doing that, by all means do it.
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van der griegen said: No to agar in the wood chips.
Not exactly. No to agar to 100% woodchips. However, if you want to do a mix of say, one part sawdust, one part woodchips and one part grain, go right ahead, just don't use 100% woodchip substrate or you may be disappointed.
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Bunya said: Is mixing a little agar with wood chips bad form? Thinking it might speed up colonization, just thinking crazy Thoughts?
Absolutely not, it's fucking excellent form, and here is the best part... I would ALWAYS add some woodchips to the sterilized substrate jar mix!!!
The reason behind this, is so the myc learns how to eat woodchips from day one, so once the myc does get to be spawned to woodchips, it's going to know wassup, and much the fuck on at those woodchips immediately!
Also, when it comes to woodlovers, grain is most likely to cause you contamination problems, if you over water and grain is still being digested, that is, you want to make sure that grain is digested before the substrate it too wet, but you should not need to worry about until you take the substrate out of the jar and it's spawned and you are tempted to water it.
Remember, you should always hydrate you substrate before hand.
Let me know if you have any more questions or anything.
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Hashed
SHROOM HEAD

Registered: 03/04/16
Posts: 1,933
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Re: Psilocybe subaeruginosa cultivation questions [Re: cronicr]
#23547409 - 08/16/16 04:36 PM (7 years, 5 months ago) |
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Quote:
cronicr said: Personally I'd ignore all that
Why?
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Hashed
SHROOM HEAD

Registered: 03/04/16
Posts: 1,933
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Re: Psilocybe subaeruginosa cultivation questions [Re: Hashed]
#23547416 - 08/16/16 04:40 PM (7 years, 5 months ago) |
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I welcome you to analyse everything I have stated, and point out what I said is incorrect.
It's all rock solid concrete shit that I stated.
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Hashed
SHROOM HEAD

Registered: 03/04/16
Posts: 1,933
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Re: Psilocybe subaeruginosa cultivation questions [Re: Hashed]
#23547460 - 08/16/16 04:56 PM (7 years, 5 months ago) |
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Let me know, cron.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Psilocybe subaeruginosa cultivation questions [Re: Hashed]
#23547479 - 08/16/16 05:06 PM (7 years, 5 months ago) |
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It's all good no worries cons on the bong hard right now
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Psilocybe subaeruginosa cultivation questions [Re: cronicr]
#23547486 - 08/16/16 05:07 PM (7 years, 5 months ago) |
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I never seen his jars till now
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Hashed
SHROOM HEAD

Registered: 03/04/16
Posts: 1,933
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Re: Psilocybe subaeruginosa cultivation questions [Re: cronicr]
#23547514 - 08/16/16 05:15 PM (7 years, 5 months ago) |
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Quote:
cronicr said: It's all good no worries cons on the bong hard right now
Told you woodlovers are my shit. 

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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Psilocybe subaeruginosa cultivation questions [Re: Hashed]
#23547519 - 08/16/16 05:16 PM (7 years, 5 months ago) |
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Hashed
SHROOM HEAD

Registered: 03/04/16
Posts: 1,933
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Re: Psilocybe subaeruginosa cultivation questions [Re: cronicr]
#23547542 - 08/16/16 05:23 PM (7 years, 5 months ago) |
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Yeah man, why not? It's a safe statement.  My knowledge of cultivation of primary wood decomposing species is quite excellent if you ask me.
 You are on the right track, Bunya, keep us updated.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Psilocybe subaeruginosa cultivation questions [Re: cronicr]
#23547547 - 08/16/16 05:24 PM (7 years, 5 months ago) |
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Quote:
Hashed said:
No point isolating wild Psilocybe subbaeruginosa myc IMO, if it is from spores from a wild print, don't even bother isolating shit...
Why not keep the genetic diversity to start off with? They are not really cubes that we want a sea of large shrooms of, it's fun to just chuck the whole agar plate in a pressure cooked jar of substrate and just let it do it's thing.
The bigger reason to not isolate is unlike cubes subs cannot be tested easily enough to justify. agar to woodchips isn't ideal because it has shitty leap off and the bigger contam concern is not grain it's the wood, wood is trichs favorite food and colonized grain is as contam resistent as it gets. you're doing great op keep it up.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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SHROOM HEAD

Registered: 03/04/16
Posts: 1,933
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Re: Psilocybe subaeruginosa cultivation questions [Re: cronicr]
#23547646 - 08/16/16 05:52 PM (7 years, 5 months ago) |
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Quote:
cronicr said:
Quote:
Hashed said:
No point isolating wild Psilocybe subbaeruginosa myc IMO, if it is from spores from a wild print, don't even bother isolating shit...
Why not keep the genetic diversity to start off with? They are not really cubes that we want a sea of large shrooms of, it's fun to just chuck the whole agar plate in a pressure cooked jar of substrate and just let it do it's thing.
The bigger reason to not isolate is unlike cubes subs cannot be tested easily enough to justify. agar to woodchips isn't ideal because it has shitty leap off and the bigger contam concern is not grain it's the wood, wood is trichs favorite food and colonized grain is as contam resistent as it gets.
Word up. That was the other point I was missing about isolating, indeed, cron. 
Well, Awakening taught me this in a spoon-feeding session. 
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On Tue May 10 2016 11:25 PM, Awakening said: Should rhizomorphic mycelium be isolated and grown out? No need, plus id assume youd want the genetic diversity to come through rather than a 'monoculture', especially in regards to surviving the elements and the test of time.Plus rhizomorphic mycelium from woodlovers often doesnt appear until it hits wood and ages a bit - then its all pretty much fat dense rhizos anyway. I sometimes have added strained soak water from wood to the agar, it works well.
You say add sawdust and wood into the grain spawn jars... How much sawdust and wood should be added into the grain spawn jars? you dont have to do this. But my logic is that they only need a small amount of grain to get started, (agar to wood doesnt work or takes forever). and by having some sawdust or fine woodchips in it, its going to get used to digesting wood during that step, and essentially allows you to skip the next step of having to spawn grain to wood in sterile jars. Ive even added small amounts of straw. I am not too sure on whats the best ratio yet, but 50/50 grain/wood or some variation of that works fine. Someone else mentioned this idea to me years ago - not my original idea.
I know how to make grain jars, but how do you prepare the sawdust and wood before being added to the grain jars? either soak it for several hours, or boil them for a few mins. and drain. smaller pieces of wood chip or using sawdust or shavings make sense here
About not watering after spawning to woodchip containers, or if the grain is not completely digested i try not to water them, just maintain the moisture. if need be these small tubs i just mist on top if required. you only need/want it to get to 90% + colonisation, as long as its mostly done before you expand it. also being that theres residual grain in there thats probably not digested at that stage, by pouring water in it you can risk harboring some kind of contam infection, plus the mycelium will be holding more nutrition in itself which makes it still a little sensitive to parasiting organisms like trichoderma - at least thats my theory.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Psilocybe subaeruginosa cultivation questions [Re: Hashed]
#23547658 - 08/16/16 05:55 PM (7 years, 5 months ago) |
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They have been eating woodchips for thousands of years...i'm sure they never forgot how to still not a bad thing or anything but still...
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Hashed
SHROOM HEAD

Registered: 03/04/16
Posts: 1,933
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Re: Psilocybe subaeruginosa cultivation questions [Re: cronicr]
#23547669 - 08/16/16 05:58 PM (7 years, 5 months ago) |
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Quote:
cronicr said: They have been eating woodchips for thousands of years...i'm sure they never forgot how to still not a bad thing or anything but still...
He means like, they will perhaps colonize faster if they are used to eating woodchips from day one, ya know.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Psilocybe subaeruginosa cultivation questions [Re: Hashed]
#23547675 - 08/16/16 05:59 PM (7 years, 5 months ago) |
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i get the idea just never really bought into it my brother accidently grew these indoors once lol
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Hashed
SHROOM HEAD

Registered: 03/04/16
Posts: 1,933
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Re: Psilocybe subaeruginosa cultivation questions [Re: cronicr]
#23547720 - 08/16/16 06:11 PM (7 years, 5 months ago) |
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Quote:
cronicr said: i get the idea just never really bought into it my brother accidently grew these indoors once lol
Which species did he accidentally grow indoors?
There are a few reports of users and users friends growing primary wood decomposing species my accident.
Ask Rainman13, he has done it indoors by accident with Psilocybe subaeruginosa before.
NothingsChanged's mate had left a Psilocybe cyanescens woodchip jar and forgot about it, found it, and it had fruited.  He even got a pic of it.
A few others have fruited woodlovers indoors before, so it can happen.
You know of anyone else doing it indoors? Always interesting to see woodlovers fruit indoors.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Psilocybe subaeruginosa cultivation questions [Re: Hashed]
#23547755 - 08/16/16 06:21 PM (7 years, 5 months ago) |
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he grew subs...they were supposed to be cyans or maybe azurs i cant' remember, i've done a few and seen many there hard but not impossible...more of a thing to do to pat yourself on the back though
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Hashed
SHROOM HEAD

Registered: 03/04/16
Posts: 1,933
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Re: Psilocybe subaeruginosa cultivation questions [Re: cronicr]
#23547767 - 08/16/16 06:24 PM (7 years, 5 months ago) |
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Quote:
cronicr said: he grew subs...they were supposed to be cyans or maybe azurs i cant' remember, i've done a few and seen many there hard but not impossible...more of a thing to do to pat yourself on the back though
Word, indeed, they are far better suited outdoors anyway. More of a novelty thing to fruit them indoors.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Psilocybe subaeruginosa cultivation questions [Re: Hashed]
#23547775 - 08/16/16 06:27 PM (7 years, 5 months ago) |
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i still talk with abert when he logs in about all kinds of shit....the guy is the boss when it comes to woodlovers.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Hashed
SHROOM HEAD

Registered: 03/04/16
Posts: 1,933
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Re: Psilocybe subaeruginosa cultivation questions [Re: cronicr]
#23547783 - 08/16/16 06:28 PM (7 years, 5 months ago) |
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IDK that user, I'll have check him out.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Psilocybe subaeruginosa cultivation questions [Re: Hashed]
#23547791 - 08/16/16 06:30 PM (7 years, 5 months ago) |
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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