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Bunya


Registered: 06/04/16
Posts: 601
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Psilocybe subaeruginosa cultivation questions
#23535331 - 08/12/16 04:11 PM (7 years, 5 months ago) |
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I have a few questions. Hoping some one can answer.
Ph range? Has anyone one done a Ph test on soil or wood in areas they are growing?
-------------------- Bunya cones kill.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Psilocybe subaeruginosa cultivation questions [Re: Bunya]
#23535369 - 08/12/16 04:23 PM (7 years, 5 months ago) |
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Fungi are some of the things along with bacteria that modify soil pH. They're the modifiers. Fungi especially mushroom forming usually tolerate a very wide range of pH. It's good to be concerned with the details but if you're having problems I doubt pH is the problem. Likewise its not the first place to start looking for success
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Bunya


Registered: 06/04/16
Posts: 601
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Re: Psilocybe subaeruginosa cultivation questions [Re: bodhisatta]
#23535393 - 08/12/16 04:30 PM (7 years, 5 months ago) |
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Quote:
Trusted Cultivator said: Fungi are some of the things along with bacteria that modify soil pH. They're the modifiers. Fungi especially mushroom forming usually tolerate a very wide range of pH. It's good to be concerned with the details but if you're having problems I doubt pH is the problem. Likewise its not the first place to start looking for success
Cheers. I was sort of wondering if i add some gypsum to the substrate will the sub mycelium like it or will it antagonise it. Thinking if they usually grow in very acidic conditions it may not be a good idea. Edit. I probably should add, that im trying it on a variety of different substrates to try and work out what works best for me.
-------------------- Bunya cones kill.
Edited by Bunya (08/12/16 04:32 PM)
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Re: Psilocybe subaeruginosa cultivation questions [Re: Bunya]
#23535421 - 08/12/16 04:40 PM (7 years, 5 months ago) |
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What's your goal? What're you working with? How far along have you come and do you have pictures? Don't worry about the pH.
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Bunya


Registered: 06/04/16
Posts: 601
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Re: Psilocybe subaeruginosa cultivation questions [Re: Adden]
#23535441 - 08/12/16 04:46 PM (7 years, 5 months ago) |
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Quote:
Adden said: What's your goal? What're you working with? How far along have you come and do you have pictures? Don't worry about the pH.
I only had 2 prints to wk with and a few stem buts on cardboard. Want to maximize growth. And inoc a few areas and do outside bed and pot. I have burried some cardbord mycelium but wanna do more.
If you have time have a look at my journal. Tells where im at now. Agar and a few jars. https://www.shroomery.org/forums/showflat.php/Number/23530056
-------------------- Bunya cones kill.
Edited by Bunya (08/12/16 04:52 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Psilocybe subaeruginosa cultivation questions [Re: Bunya]
#23535816 - 08/12/16 07:00 PM (7 years, 5 months ago) |
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PH is not a concern as it is outdoors anyway
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Bunya


Registered: 06/04/16
Posts: 601
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Re: Psilocybe subaeruginosa cultivation questions [Re: cronicr]
#23536064 - 08/12/16 08:26 PM (7 years, 5 months ago) |
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Quote:
cronicr said: PH is not a concern as it is outdoors anyway
The sterile jars are indoors ? But thanks for your time.
-------------------- Bunya cones kill.
Edited by Bunya (08/12/16 08:28 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Psilocybe subaeruginosa cultivation questions [Re: Bunya]
#23536072 - 08/12/16 08:29 PM (7 years, 5 months ago) |
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ph is a concern in casing layers that do not colonize....jars do
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Bunya


Registered: 06/04/16
Posts: 601
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Re: Psilocybe subaeruginosa cultivation questions [Re: cronicr]
#23536105 - 08/12/16 08:38 PM (7 years, 5 months ago) |
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Would the addition of Gypsum in the next lot of jars i do Like these ones assist in colonization? With Psilocybe subaeruginosa mycelium grown on agar.
. Cheers
-------------------- Bunya cones kill.
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Psilicon
Really Nice Guy

Registered: 08/26/12
Posts: 7,057
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Re: Psilocybe subaeruginosa cultivation questions [Re: Bunya]
#23536643 - 08/12/16 11:50 PM (7 years, 5 months ago) |
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I'd consider a pinch in the soaking water, but not much. It isn't crucial, since they grow on wood chips that aren't soaked in gypsum water pretty well in the wild.
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 10 hours, 54 minutes
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Re: Psilocybe subaeruginosa cultivation questions [Re: Bunya]
#23537097 - 08/13/16 05:47 AM (7 years, 5 months ago) |
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Quote:
Bunya said: Would the addition of Gypsum in the next lot of jars i do Like these ones assist in colonization? With Psilocybe subaeruginosa mycelium grown on agar.
. Cheers
Yes, Makes things less sticky, but perfect moisture content is most crucial after you get good clean spawn on agar to use.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Bunya


Registered: 06/04/16
Posts: 601
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Re: Psilocybe subaeruginosa cultivation questions [Re: wtfcrazymofo]
#23542817 - 08/15/16 02:37 AM (7 years, 5 months ago) |
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Cereal pest Sorry guys any thoughts on these.
 Spore on agar Where would you take transfer from?
Cardboard mycelium isolated Thoughts?

Transfer from cardboard isolate Any thought?

Any thoughts opinions? Wanna learn
-------------------- Bunya cones kill.
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liloldme
( ͝° ͜ʖ͡°)つ=D



Registered: 05/15/04
Posts: 5,087
Loc: Zone 8
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Re: Psilocybe subaeruginosa cultivation questions [Re: Bunya]
#23542895 - 08/15/16 04:02 AM (7 years, 5 months ago) |
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try more agar transfers that contain a little less nutrients. I think that will help you isolate the more rizomorphic growth you're seeking. it tends to stay very fuzzy and grow slow when nutrient levels are too high. Welcome to the club
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Bunya


Registered: 06/04/16
Posts: 601
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Re: Psilocybe subaeruginosa cultivation questions [Re: liloldme]
#23542904 - 08/15/16 04:23 AM (7 years, 5 months ago) |
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Quote:
liloldme said: try more agar transfers that contain a little less nutrients. I think that will help you isolate the more rizomorphic growth you're seeking. it tends to stay very fuzzy and grow slow when nutrient levels are too high. Welcome to the club 
Thanks will def try that with next lot of dishes soon. Its agar, potato powder, glucose n a little yeast. Should i cut back on all nutrients or just some? Do you think its fine to make LC as is or best to get more rizomorphic growth? Thanks for your help.
-------------------- Bunya cones kill.
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liloldme
( ͝° ͜ʖ͡°)つ=D



Registered: 05/15/04
Posts: 5,087
Loc: Zone 8
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Re: Psilocybe subaeruginosa cultivation questions [Re: Bunya]
#23544199 - 08/15/16 02:01 PM (7 years, 5 months ago) |
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I would transfer it a couple more times to play it safe.these are the ratios I use https://www.shroomery.org/5243/What-are-some-good-agar-media-recipes
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Psilicon
Really Nice Guy

Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
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Re: Psilocybe subaeruginosa cultivation questions [Re: liloldme]
#23545611 - 08/15/16 10:40 PM (7 years, 5 months ago) |
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As far as transfers go, all your sectors looked about the same except for in the last picture. In that I would have taken from 9:30.
Excellent, clean-looking growth. Great job so far.
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Bunya


Registered: 06/04/16
Posts: 601
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Re: Psilocybe subaeruginosa cultivation questions [Re: Psilicon]
#23545876 - 08/16/16 01:30 AM (7 years, 5 months ago) |
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Quote:
liloldme said: I would transfer it a couple more times to play it safe.these are the ratios I use https://www.shroomery.org/5243/What-are-some-good-agar-media-recipes
Thanks great link. Yes will be transfering lots as i started out with very little.want to bulk up on it and try everything i can think of or sugested.
Quote:
van der griegen said: As far as transfers go, all your sectors looked about the same except for in the last picture. In that I would have taken from 9:30.
Excellent, clean-looking growth. Great job so far.
Cheers, I will transfer that bit.
Is mixing a little agar with wood chips bad form? Thinking it might speed up colonization, just thinking crazy Thoughts?
-------------------- Bunya cones kill.
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Psilicon
Really Nice Guy

Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
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Re: Psilocybe subaeruginosa cultivation questions [Re: Bunya]
#23546471 - 08/16/16 10:05 AM (7 years, 5 months ago) |
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No to agar in the wood chips.
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Hashed
SHROOM HEAD

Registered: 03/04/16
Posts: 1,933
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Re: Psilocybe subaeruginosa cultivation questions [Re: Psilicon]
#23547329 - 08/16/16 04:09 PM (7 years, 5 months ago) |
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Sorry for taking a while to get back to you mate, I had other shit to do. Thank you for requesting I take a look, Bunya, this is my shit. 
No point isolating wild Psilocybe subbaeruginosa myc IMO, if it is from spores from a wild print, don't even bother isolating shit...
Why not keep the genetic diversity to start off with? They are not really cubes that we want a sea of large shrooms of, it's fun to just chuck the whole agar plate in a pressure cooked jar of substrate and just let it do it's thing.
That rhizomorpic myc does not show until it hits the substrate and ages a bit, so more often than not P. subaeruginosa myc starts out real fluffy.
If you are wanting to make myc from wild wood lover mushrooms, I have a fucking excellent tek for that.
From spores, it's not bad what you have been doing, playing with pressure cooked substrate.
Once a jar of your pressure cooked substrate material is 90% colonized, you can spawn in to woodchips to grow out, no problem, in a container, in a large tub to fruit, or in an outdoor woodchip bed, who cares, that's up to you.
And at that point it's absolutely fine to leave it out in the elements, so once a sterilized jar is at 90% just crack it open, it's fine to spawn it to non-sterilized woodchips afterwards, and I hear that a bit of dirt is always good to mix through your virgin woodchips, think of the substrate of a real wild P. subaeruginosa patch... Woodchips AND drit!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Psilocybe subaeruginosa cultivation questions [Re: Hashed]
#23547353 - 08/16/16 04:18 PM (7 years, 5 months ago) |
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Personally I'd ignore all that
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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