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InvisibleBoogieman47
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how thick should the agar be when sandwiching a contaminated tissue?
    #23533019 - 08/11/16 09:52 PM (7 years, 6 months ago)

I've only ever done warm pours which suck and my first two attempts at a sandwich failed my agar was maybe an 1/16-1/8 at most should I make it like 1/4 -1/2??


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Invisibleazur
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Re: how thick should the agar be when sandwiching a contaminated tissue? [Re: Boogieman47]
    #23533058 - 08/11/16 10:03 PM (7 years, 6 months ago)

Sandwiching is for cleaning up bacteria, not other contams.
Pour antibiotic agar over it. As thick as it needs to be to cover it. As soon as the healthy myc pops through, transfer.


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InvisibleBoogieman47
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Re: how thick should the agar be when sandwiching a contaminated tissue? [Re: azur]
    #23533111 - 08/11/16 10:16 PM (7 years, 6 months ago)

So molds and other weird shit like that use antibiotic agar, and if it's only bacteria then I can just sandwich ?? But either way the thick the better ??


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Invisibleazur
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Re: how thick should the agar be when sandwiching a contaminated tissue? [Re: Boogieman47]
    #23533141 - 08/11/16 10:24 PM (7 years, 6 months ago)

Molds and other weird shit like that use antibiotic agar? Yes

If it's only bacteria then I can just sandwich ??
Yes, with antibiotic agar.

But either way the thick the better ?? Not necessarily. I like thicker so I csn pick it up better.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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InvisibleBoogieman47
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Registered: 03/05/16
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Re: how thick should the agar be when sandwiching a contaminated tissue? [Re: azur]
    #23533172 - 08/11/16 10:31 PM (7 years, 6 months ago)

OK I appreciate it man I'll pick up some antibiotics tomorrow


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