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Offlinesshayne
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Darkening color of grain in incubation. Contamination?
    #23532876 - 08/11/16 09:07 PM (7 years, 5 months ago)

Hey guys, I have recently brought 2 bags of pre sterilized grain from a Australian mushroom site.  I have injected one of the bags with 4ccs of golden teacher spores and put it in the incubator, with the other bag I planed to store for a later date.

1week later and the color of the injected bag has turned a dark brown, is this normal? Or is it an contaminated ? 


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Invisibleazur
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Re: Darkening color of grain in incubation. Contamination? [Re: sshayne]
    #23532889 - 08/11/16 09:13 PM (7 years, 5 months ago)

No. Contaminated. Throw away


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Invisiblemycohumanity
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Re: Darkening color of grain in incubation. Contamination? [Re: sshayne]
    #23532890 - 08/11/16 09:13 PM (7 years, 5 months ago)

That definitely does not look right.  I would toss it if it was mine.


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InvisibleBoogieman47
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Re: Darkening color of grain in incubation. Contamination? [Re: mycohumanity]
    #23532907 - 08/11/16 09:19 PM (7 years, 5 months ago)

Your syringe could be bad the only way to find out is with agar really ... did you flame sterilize the needle till it was red hot then squirt a couple drops out to cool ?


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InvisibleInocuole
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Re: Darkening color of grain in incubation. Contamination? [Re: Boogieman47]
    #23532922 - 08/11/16 09:22 PM (7 years, 5 months ago)

Another vote for ew wtf get that out of there.


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Offlinesshayne
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Re: Darkening color of grain in incubation. Contamination? [Re: Inocuole]
    #23532976 - 08/11/16 09:39 PM (7 years, 5 months ago)

Thanks guys,  it's going in the bin right away. And no I didn't flame the needle I only used alcohol in my sterile procedure, I will be sure to flame the needle next time thanks.


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InvisibleBoogieman47
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Re: Darkening color of grain in incubation. Contamination? [Re: sshayne]
    #23532983 - 08/11/16 09:42 PM (7 years, 5 months ago)

That could of been the problem make sure the tip of the needle is red then you only need a drop or two to cool it, it will sizzle while heating it so don't trip ... look into doing agar also man that will make that syringe last a long time


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