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ihatethis
Slightly Confused

Registered: 09/08/10
Posts: 521
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LC - does this look ready?
#23531179 - 08/11/16 11:05 AM (7 years, 5 months ago) |
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Here is a pint jar of KSSS LC. Made with organic honey.
I've never done LC before and I think this looks ready. I would love to make some jars with this. What do you guys think? I do see a few small dots that aren't white, but only when I shake it up. I think this is just dirt or mineral deposits from the water?

Is this:
1. Ready 2. Not ready 3. Contaminated
Thanks in advance!
Edited by ihatethis (08/11/16 11:06 AM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: LC - does this look ready? [Re: ihatethis]
#23531222 - 08/11/16 11:20 AM (7 years, 5 months ago) |
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I don't like the look of those bubbles. It can stand to get a lot more colonized as well.
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ihatethis
Slightly Confused

Registered: 09/08/10
Posts: 521
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Re: LC - does this look ready? [Re: Pastywhyte]
#23531285 - 08/11/16 11:42 AM (7 years, 5 months ago) |
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The bubbles are from a shake I just did. I shook it, waited a few minutes and then took the picture.
Ok so I should wait until I see white in the whole jar? In cybers LC tek it states that once you see the white, it is ready. Thanks for the input.
https://www.shroomery.org/10477/Cybers-Liquid-Culture-Tek
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Psykronik
OverGrower


Registered: 02/28/12
Posts: 150
Loc: Scotland
Last seen: 7 years, 4 months
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Re: LC - does this look ready? [Re: ihatethis]
#23531339 - 08/11/16 12:02 PM (7 years, 5 months ago) |
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My honey LC has a lot of little bits of crap floating around. It's just sediment from the honey. How did you inoculate your LC ?
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ihatethis
Slightly Confused

Registered: 09/08/10
Posts: 521
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Re: LC - does this look ready? [Re: Psykronik]
#23531429 - 08/11/16 12:35 PM (7 years, 5 months ago) |
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Because I'm a semi-newb, I used the oven tek. I got some criticism for doing so, even though it looks like everything worked out ok.
In future inoculations for LC I'm going to use a glove box
Edited by ihatethis (08/11/16 12:36 PM)
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FractalXplora
Grainiack




Registered: 02/11/06
Posts: 2,494
Loc: UK
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Re: LC - does this look ready? [Re: ihatethis]
#23531441 - 08/11/16 12:39 PM (7 years, 5 months ago) |
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Thats the thing with an LC, you cant really tell if its contaminated until you knock some jars up with it!Good luck!
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Psykronik
OverGrower


Registered: 02/28/12
Posts: 150
Loc: Scotland
Last seen: 7 years, 4 months
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Re: LC - does this look ready? [Re: ihatethis]
#23531446 - 08/11/16 12:40 PM (7 years, 5 months ago) |
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Sorry, I meant did you use a MS syringe or wedge of agar or a colonised grain to inoculate your jar
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ihatethis
Slightly Confused

Registered: 09/08/10
Posts: 521
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Re: LC - does this look ready? [Re: Psykronik]
#23531480 - 08/11/16 12:52 PM (7 years, 5 months ago) |
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You're cool. I used a syringe for these, and for two others I did with colonized WBS.
Trying a ton of different techniques this time.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: LC - does this look ready? [Re: ihatethis]
#23531548 - 08/11/16 01:11 PM (7 years, 5 months ago) |
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MS syringe AND oven tek, so yeah, criticized a little perhaps. And you're still under the delusion that everything "worked out okay"?
Just you wait..
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Psykronik
OverGrower


Registered: 02/28/12
Posts: 150
Loc: Scotland
Last seen: 7 years, 4 months
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Re: LC - does this look ready? [Re: ihatethis]
#23531554 - 08/11/16 01:12 PM (7 years, 5 months ago) |
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I would be very dubious about the spore syringe LC and inoculating in the oven...
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ihatethis
Slightly Confused

Registered: 09/08/10
Posts: 521
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Re: LC - does this look ready? [Re: Psykronik]
#23531582 - 08/11/16 01:21 PM (7 years, 5 months ago) |
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It looks ok, I never said it was ok. It isn't a delusion if I'm not sure yet...
Inocule, I feel like you just plain don't like me. Did I do something to you? I'm just trying to grow and I feel like you are pushing me away...
Aren't we all in it for the PNL (Peace and love)?
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ihatethis
Slightly Confused

Registered: 09/08/10
Posts: 521
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Re: LC - does this look ready? [Re: ihatethis]
#23531608 - 08/11/16 01:28 PM (7 years, 5 months ago) |
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I'm going to follow that up by saying again: most of this is all new to me. Most of the teks I'm using this time around are 100% brand new to me. Learning isn't immediate - for anyone. I'm just looking for help, and I feel like you just beat me when I'm down.
It's just plain not nice and makes me want to not post here anymore. I'm not an idiot, I'm just ignorant of a lot of the "better" ways of doing things. I ask for help, but then I get spit on.
This site was friendly a few years ago.
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Psykronik
OverGrower


Registered: 02/28/12
Posts: 150
Loc: Scotland
Last seen: 7 years, 4 months
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Re: LC - does this look ready? [Re: ihatethis]
#23531629 - 08/11/16 01:35 PM (7 years, 5 months ago) |
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Chill man. I think your just mistaking his sarcasm for impoliteness. By the looks of your sweet PE monotub sig. Whatever your doing is working for you. I would just keep doing what your doing if it's working out. Minus the MS to LC ofcourse
Edited by Psykronik (08/11/16 01:37 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: LC - does this look ready? [Re: Psykronik]
#23531809 - 08/11/16 02:54 PM (7 years, 5 months ago) |
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And the oven tek...
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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Re: LC - does this look ready? [Re: ihatethis]
#23531825 - 08/11/16 03:05 PM (7 years, 5 months ago) |
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I don't think anyone really dislikes you or is upset. It does seem like you're using a ton of old or bad info though. Mentioning a glovebox, MS to LC, oven tek?! Refer to section 1. of this post: https://www.shroomery.org/forums/showflat.php/Number/22861379
Spore syringes are often also full of stuff that isn't cube spores. This means that if you go to LC directly from a syringe or a print you're highly likely to be growing bacteria as well.
I've made plenty of LCs before and believe me, you will hardly ever be able to see contams until it's on grains or something. Literally I've only been able to see contams in LC once and it was because there was no myc in the LC, just a ton of bacillus.
If I were you, I'd wait a while so it can colonize longer then I'd shoot up a small test jar of some grains or a PF cake if you'd rather. Even then bacteria can hide from you, which is why agar is so handy.
Edited by Greg (08/11/16 03:12 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: LC - does this look ready? [Re: Greg]
#23531997 - 08/11/16 04:10 PM (7 years, 5 months ago) |
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Wanting you to grow more mushrooms for your efforts is pretty friendly to me. Especially for free. When you're growing felonies we assume you're an adult an can take some no nonsense advice. We're not the ones that looked up 10 year old threads or threads with no results or replications.
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ihatethis
Slightly Confused

Registered: 09/08/10
Posts: 521
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Re: LC - does this look ready? [Re: bodhisatta]
#23532271 - 08/11/16 05:32 PM (7 years, 5 months ago) |
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I would agree that it would be sarcasm if he didn't this type of stuff on each and every thread I have posted here for several weeks.
The first thing I heard from him:
"What the fuck?
Seriously? This is how you inoculate? Please do not ever make any teks here."
Is that sarcasm? No, I think he just has a problem with my process. Everyone has an opinion, but he / she / they have just been plain rude to me since I started back up here about a month ago.
The teks I used for this are:
https://www.shroomery.org/10477/Cybers-Liquid-Culture-Tek https://www.shroomery.org/forums/showflat.php/Number/15034407 https://www.shroomery.org/9666/Cybers-Self-Healing-Injection-point-lids
The only thing that came from experience was the "oven tek" inoculation that I actually never read about before doing it. I actually learned this from experience when the guy that taught me to grow (about 8 years ago) did this with his WBS Hpoo grow with enormous success. He paid off his semester with results from the oven tek. Honestly have always used it because it just works for me. I am a clean freak and my house is always spotless so maybe that's why I have had so much success?
Trust me, in the future I am only using an SAB / GB for these types of inoculations. I will post the result of my jars inoculated with LC after I let it simmer for a few more days.
Thanks again for the responses.
Edited by ihatethis (08/11/16 05:54 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: LC - does this look ready? [Re: ihatethis]
#23532586 - 08/11/16 07:32 PM (7 years, 5 months ago) |
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Paid off his semester with results from the oven tek... 
Read the rules. http://www.shroomery.org/forums/showflat.php/Number/14960167
Also I'm putting that LC tek you just linked in the archive where it belongs.. I can't touch the honey LC one but I'd trash that one too if I could. Quit making LC with spores, it's not a good idea, it's no fun to troubleshoot, for us, or for you.
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