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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Is it normal for spore syringes to look like this? [Re: ijustwantobehuman]
#23531044 - 08/11/16 10:29 AM (7 years, 5 months ago) |
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Okay so, once or twice, I've seen APE work on cakes. The other dozen times... it always looked like a bunch of sad dry aborts. APE is a very thirsty variety of cubensis, and cakes don't provide much water. Good luck at any rate, but I would really either work on getting the APE to a bulk substrate or do a Muda bottle grow, agar, something. APE syringes are dirty as all hell, very bacterial because of how they're made. Should hopefully work on a cake but you won't be able to use it on grains or other grain-based substrate.
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ijustwantobehuman
MastoDAAAMN

Registered: 03/03/15
Posts: 64
Last seen: 1 year, 10 days
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Re: Is it normal for spore syringes to look like this? [Re: mrmazdarx9]
#23531045 - 08/11/16 10:29 AM (7 years, 5 months ago) |
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Quote:
mrmazdarx9 said: Hope you used sterile techniques not just wiped the syringe on your top or what ever your doing there
lol I hope so too.
-------------------- Let The Rabbits Wear Glasses
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Is it normal for spore syringes to look like this? [Re: ijustwantobehuman]
#23531052 - 08/11/16 10:30 AM (7 years, 5 months ago) |
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Quote:
mrmazdarx9 said: Hope you used sterile techniques not just wiped the syringe on your top or what ever your doing there
Think that's just the luer lock plug.. Still. I wouldn't be touching that much of my syringe on non-sterile material. Should really only be opening and using it inside a SAB, and then put it back inside a container or bag so minimal shit lands on it. I mean, sometimes people use one spore syringe per grow, or even more than one syringe for an entire grow, and I guess you can be careless then, but eventually a spore syringe lasts you forever because you never use more than a couple drops out of it to make infinite inoculations, so you'd want to take better care of it so it's still viable a year or two down the line.
At least without like, cleaning the shit out of the outside of the syringe, which could lead to accidents involving compromising the whole thing.
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: Is it normal for spore syringes to look like this? [Re: Inocuole]
#23531060 - 08/11/16 10:32 AM (7 years, 5 months ago) |
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Ah right thought for a second he was shooting up in the belly
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ijustwantobehuman
MastoDAAAMN

Registered: 03/03/15
Posts: 64
Last seen: 1 year, 10 days
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Re: Is it normal for spore syringes to look like this? [Re: Inocuole]
#23531067 - 08/11/16 10:33 AM (7 years, 5 months ago) |
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Quote:
Inocuole said: Okay so, once or twice, I've seen APE work on cakes. The other dozen times... it always looked like a bunch of sad dry aborts. APE is a very thirsty variety of cubensis, and cakes don't provide much water. Good luck at any rate, but I would really either work on getting the APE to a bulk substrate or do a Muda bottle grow, agar, something. APE syringes are dirty as all hell, very bacterial because of how they're made. Should hopefully work on a cake but you won't be able to use it on grains or other grain-based substrate.
Only using this one cake to see how it responds before shooting up 6 jars of WBS for my 66qt mono.. Just lost 12 jars on two occasions using a bad syringe. Lots of wasted time and money BUT a good learning experience.
-------------------- Let The Rabbits Wear Glasses
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Is it normal for spore syringes to look like this? [Re: ijustwantobehuman]
#23531077 - 08/11/16 10:34 AM (7 years, 5 months ago) |
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Do not shoot APE spores directly to WBS. The cake is no indication of whether or not those spores will do well on grain. Cakes can work past a certain amount of bacteria, but in my experience, and tons of others, the bacteria in APE syringes often completely wrecks all the grain you throw it at until you clean it up on agar.
Mine was so dirty, I couldn't germinate it on agar, I had to germinate it on grain, then drop a colonized (but bacterial) grain on agar, and transfer clean growth away from the bacteria. None of the grain jars would ever finish if left to their own devices, they'd just get steadily more slimey and gross looking, wouldn't recover from shakes, etc.
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ijustwantobehuman
MastoDAAAMN

Registered: 03/03/15
Posts: 64
Last seen: 1 year, 10 days
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Re: Is it normal for spore syringes to look like this? [Re: Inocuole]
#23531112 - 08/11/16 10:40 AM (7 years, 5 months ago) |
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Thanks for the knowledge drop. Well I might just try it on a couple jars just for fun and see what happens, I'll keep the syringe around for when I study agar.
-------------------- Let The Rabbits Wear Glasses
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Is it normal for spore syringes to look like this? [Re: ijustwantobehuman]
#23531531 - 08/11/16 01:05 PM (7 years, 5 months ago) |
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Just be sure to save some of it. Any way you can salvage it, it's a great variety to grow, it's just.. very finicky.
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KThunderland
Dude


Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 2 months
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Re: Is it normal for spore syringes to look like this? [Re: Inocuole]
#23533084 - 08/11/16 10:10 PM (7 years, 5 months ago) |
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Quote:
Inocuole said: Do not shoot APE spores directly to WBS. The cake is no indication of whether or not those spores will do well on grain. Cakes can work past a certain amount of bacteria, but in my experience, and tons of others, the bacteria in APE syringes often completely wrecks all the grain you throw it at until you clean it up on agar.
Mine was so dirty, I couldn't germinate it on agar, I had to germinate it on grain, then drop a colonized (but bacterial) grain on agar, and transfer clean growth away from the bacteria. None of the grain jars would ever finish if left to their own devices, they'd just get steadily more slimey and gross looking, wouldn't recover from shakes, etc.
this is exactly how i do it to the t. Well said.
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