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Offlinephangtonpower
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Stalled mono tub...what to do
    #23529565 - 08/10/16 08:37 PM (7 years, 5 months ago)

So I started a nano tub about 4 weeks ago. It's only about 1.5 liters. I used 3 grated BRF cakes as spawn mixed with pasteurized Hpoo and gypsum.

The thing is when I first put the tub together, I didn't make enough bulk. I made some the next day and mixed it in. I guess I didn't mix it in well enough because only about 60 to 70 % is colonized at the top half inch. The whole tub is about 90 to 95% colonized other wise. It's been 4 weeks no contams, the bottom colonized and probably consolidated by now, and actually the top parts that are colonized are starting to show primordia.

What would you guys do? wait and see if it colonizes, cut the top part off, 24 hour dunk, roll in vermiculite, and stick it in a SGFC like a big brf cake?


Edited by phangtonpower (08/11/16 02:15 AM)


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Invisibleweetsie
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Re: Stalled mono tub...what to do [Re: phangtonpower]
    #23529689 - 08/10/16 09:09 PM (7 years, 5 months ago)

If I saw primordia I'd let it fruit.


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Offlinephangtonpower
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Re: Stalled mono tub...what to do [Re: weetsie]
    #23529726 - 08/10/16 09:21 PM (7 years, 5 months ago)

But would you cut the top part off and fruit it in SGFC or leave it as it is with a mostly uncolonized surface and use the monotub?


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InvisibleInocuole
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Re: Stalled mono tub...what to do [Re: phangtonpower]
    #23529749 - 08/10/16 09:27 PM (7 years, 5 months ago)

You're cutting what off now?  Explain your thought process here...


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Offlinephangtonpower
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Re: Stalled mono tub...what to do [Re: Inocuole]
    #23529751 - 08/10/16 09:28 PM (7 years, 5 months ago)

The top half inch or so that hasn't colonized.


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Re: Stalled mono tub...what to do [Re: phangtonpower]
    #23529753 - 08/10/16 09:28 PM (7 years, 5 months ago)

For what purpose?  I've just never heard of this before, you'll have to excuse my ignorance.


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Offlinephangtonpower
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Re: Stalled mono tub...what to do [Re: Inocuole]
    #23529762 - 08/10/16 09:31 PM (7 years, 5 months ago)

as stated in the op it's been sitting for 4 weeks with the top half inch not colonized completely.


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Re: Stalled mono tub...what to do [Re: phangtonpower]
    #23529773 - 08/10/16 09:36 PM (7 years, 5 months ago)

Yeah, but that's probably because of an issue with the conditions.  I don't see why you would want to chop off the whole pinning surface to ensure that it can't fruit.  It would have to completely rebuild the damage to the network before it could even think about starting to produce pins, easily setting you back another week or so.  On the other hand the stress would be almost guaranteed to cause some side pins to form...


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Offlinephangtonpower
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Re: Stalled mono tub...what to do [Re: Inocuole]
    #23529815 - 08/10/16 09:49 PM (7 years, 5 months ago)

But if I fruit in the condition it is in now, I'm worried about the uncolonized areas contaminating.

I'm in no rush to fruit this and can wait longer if there is still a chance that the top might colonize, but after 4 weeks I'm thinking the top is dry.

That's why I'm thinking of cutting the uncolonized area and putting it into my SGFC.

If I went this way do you guys think I should treat it like a big cake? Dunk 24 hours and roll in verm.


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Offlinephangtonpower
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Re: Stalled mono tub...what to do [Re: phangtonpower]
    #23534138 - 08/12/16 09:51 AM (7 years, 5 months ago)

Does anyone think it would be ok to turn it into a big cake and how I would do it?


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Re: Stalled mono tub...what to do [Re: phangtonpower]
    #23534153 - 08/12/16 09:58 AM (7 years, 5 months ago)

if you say it's dry on top, give it a mist or something? :shrug:

pictures might help.


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Re: Stalled mono tub...what to do [Re: phangtonpower]
    #23534212 - 08/12/16 10:14 AM (7 years, 5 months ago)

.


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Edited by amidogen (04/19/18 09:20 AM)


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Offlinephangtonpower
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Re: Stalled mono tub...what to do [Re: amidogen]
    #23536085 - 08/12/16 08:33 PM (7 years, 5 months ago)

Quote:

mupetmower said:
if you say it's dry on top, give it a mist or something? :shrug:

pictures might help.




I don't really know if it's dry on top since I haven't opened it. I'm assuming that it's dry since it's been 4 weeks. Do you think I should open it up and give it a mist?

best I can do with picks. Don't want to open it up because I'm scared to contam it. Coke can for reference.

In the first pic you can see it's about 90 95% colonized. Been like that for maybe 2 weeks now.



kinda of hard to tell, but hopefully you guys can see at the top left it is not colonized completely.



Quote:

amidogen said:
Quote:

phangtonpower said:
Does anyone think it would be ok to turn it into a big cake and how I would do it?



I think you're asking for trouble with this. If Inoc's never heard of someone trying this, it's pretty far out there. Doing things that are pretty far out there usually lead to bad news bears. You're going to have two extremely stressed pieces of mycelium if you do this.

I'd follow Inoc and mupet's advice. Put up some pictures and let's focus on your conditions and getting your tub to fruit like a tub.


|

I swear I've read people washing uncolonized parts on cakes, I thought maybe It wouldn't be a problem with this.

Anyway so far the only suggestion is to fruit it with the uncolonized top. I really don't want to contam exposed bulk.

Thanks for the help.


Edited by phangtonpower (08/12/16 08:34 PM)


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InvisibleInocuole
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Re: Stalled mono tub...what to do [Re: phangtonpower]
    #23536270 - 08/12/16 09:31 PM (7 years, 5 months ago)

You haven't opened that for 4 weeks?


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Offlinephangtonpower
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Re: Stalled mono tub...what to do [Re: Inocuole]
    #23536292 - 08/12/16 09:40 PM (7 years, 5 months ago)

No sir.


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InvisibleLocN9ne
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Re: Stalled mono tub...what to do [Re: phangtonpower]
    #23536723 - 08/13/16 12:32 AM (7 years, 5 months ago)

Dude fruit that sumbitch... Thats all you can really do... The air won't contaminate it, but I think if it's been this long and hasn't started fruiting already that it probably won't.


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Offlinephangtonpower
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Re: Stalled mono tub...what to do [Re: LocN9ne]
    #23536742 - 08/13/16 12:47 AM (7 years, 5 months ago)

So fruit it as is in the tub?

If that's the only option will do.

I made 6 cakes all together. Three I used in the tub the other 3 in a SGFC.

The 2 of the 3 cakes in SGFC contamed as they were producing, so threw them out. The last cakes, which had a little more rolled verm just started fruiting a few days ago. Strain is B+. I'm worried that the uncolonized part will contaminate before it actually fruits.

I feel this thing is doomed, but I guess I'll try and fruit it in the tub.


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Re: Stalled mono tub...what to do [Re: LocN9ne]
    #23536769 - 08/13/16 01:06 AM (7 years, 5 months ago)

not being funny but how the fuck you gonna fruit that as a tub, you only got an inch above the substrate :crankey:


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Re: Stalled mono tub...what to do [Re: weetsie]
    #23536772 - 08/13/16 01:08 AM (7 years, 5 months ago)

That sub is wayyyy too thick, anything over 3 inches is pushing it


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Offlinephangtonpower
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Re: Stalled mono tub...what to do [Re: Psilosoulful]
    #23536793 - 08/13/16 01:23 AM (7 years, 5 months ago)

Quote:

weetsie said:
not being funny but how the fuck you gonna fruit that as a tub, you only got an inch above the substrate :crankey:




? What do you mean? The substrate is 4 inches. What do you suggest I do then? a few people say to fruit as is and only parts of the top 1/2 inch haven't colonized.

Quote:

Psilosoulful said:
That sub is wayyyy too thick, anything over 3 inches is pushing it




From what I've researched 4 inches is perfect depth.


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Invisibleweetsie
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Re: Stalled mono tub...what to do [Re: phangtonpower]
    #23536854 - 08/13/16 02:08 AM (7 years, 5 months ago)

I figured you were talking about fruiting it in the tub, as in a mono tub but maybe you're talking about putting that tub in the SGFC with the lid off or something.

I said what I think you should do 2 days ago, you have primordia, fruit it! washing away uncolonized sub is not the same with bulk, with a BRF cake inoculated at only a few points, the mycelium grows from A to B in a sense so when you have a small patch of uncolonized substrate you can wash it away and be confident the rest of the cake is colonized.


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Offlinephangtonpower
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Re: Stalled mono tub...what to do [Re: weetsie]
    #23536878 - 08/13/16 02:24 AM (7 years, 5 months ago)

This is actually what I'm confused about if I fruit it. I would either use the monotub to fruit or take it out of the tub remove the plastic and treat it as a cake, but if I did this should I case it with verm like a cake.

If i did the mono tub I would just stuff the holes with poly fill and hope for the best. If I stuck it in a SGFC, originally I was thinking slicing the top half inch off, removing the cake out of the tub, and sticking it into my SGFC, but now if I did it this way, from what I'm gathering, is just remove it from the tub with the uncolnized part as it is a stick it in into the SGFC?


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InvisibleLocN9ne
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Re: Stalled mono tub...what to do [Re: phangtonpower]
    #23536917 - 08/13/16 02:54 AM (7 years, 5 months ago)

Don't remove ANYTHING... Not the bucket it's in, not a 1/2" off the top, not the uncolonized parts,nothing... Set that whole bucket thing in your SGFC and let it fruit.


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Edited by LocN9ne (08/13/16 02:54 AM)


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Offlinephangtonpower
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Re: Stalled mono tub...what to do [Re: LocN9ne]
    #23536923 - 08/13/16 02:58 AM (7 years, 5 months ago)

Thanks for clarifying things :smile:
See how it goes!!


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Offlinephangtonpower
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Re: Stalled mono tub...what to do [Re: weetsie]
    #23536947 - 08/13/16 03:25 AM (7 years, 5 months ago)

Quote:

weetsie said:
not being funny but how the fuck you gonna fruit that as a tub, you only got an inch above the substrate :crankey:




Just got what you were getting at. It's actually a dub tub except I modified another tub and cut the lid where only the rim was left, flipped over and glued the tub part to rim. That way when I have the two halves together it snaps together and is more secure than a regular dub tub.

I usually have the regular lid on it when colonizing. There is way less than an inch for fruiting otherwise :P


Edited by phangtonpower (08/13/16 03:29 AM)


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Re: Stalled mono tub...what to do [Re: phangtonpower]
    #23537149 - 08/13/16 06:48 AM (7 years, 5 months ago)

Quote:

phangtonpower said:
Quote:

weetsie said:
not being funny but how the fuck you gonna fruit that as a tub, you only got an inch above the substrate :crankey:




Just got what you were getting at. It's actually a dub tub except I modified another tub and cut the lid where only the rim was left, flipped over and glued the tub part to rim. That way when I have the two halves together it snaps together and is more secure than a regular dub tub.

I usually have the regular lid on it when colonizing. There is way less than an inch for fruiting otherwise :P



Don't forget to drill some holes in the top tub and stuff with polyfill,
you want to make sure there's adequate FAE in there


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Offlinephangtonpower
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Re: Stalled mono tub...what to do [Re: Psilosoulful]
    #23538361 - 08/13/16 04:06 PM (7 years, 5 months ago)

Already done :smile:


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