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OfflineFreeTheSoul
The wonderer.
Registered: 01/04/14
Posts: 2,297
Last seen: 2 years, 2 months
Culture slants
    #23528150 - 08/10/16 12:50 PM (7 years, 5 months ago)

When you inoculate a culture slant are you supposed to leave the lid on the test tube slightly ajar and wrap in parafilm until it is colonized, and once it is colonized do you close the lid when you put it into storage or do you leave parafilm on while lid is still slightly ajar and put into a ziplock bag for storage?


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InvisibleMarty Mycfly
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Registered: 12/16/13
Posts: 976
Re: Culture slants [Re: FreeTheSoul]
    #23528232 - 08/10/16 01:25 PM (7 years, 5 months ago)

I have done all of those combinations, I find it best to inoculate and leave the lid slightly loose then wrap in para film, let it colonize for a few days and then into the baggies and fridge.
Tubes that I stored with lid tight seemed to not keep as good as the ones with a little GE.


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Edited by Marty Mycfly (08/10/16 01:27 PM)


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OfflineFreeTheSoul
The wonderer.
Registered: 01/04/14
Posts: 2,297
Last seen: 2 years, 2 months
Re: Culture slants [Re: Marty Mycfly]
    #23528292 - 08/10/16 01:48 PM (7 years, 5 months ago)

Ty man I figured it was best to do it that way just wasn't sure, left you a rating for the help. :cheers:


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InvisibleMarty Mycfly
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Registered: 12/16/13
Posts: 976
Re: Culture slants [Re: FreeTheSoul]
    #23528297 - 08/10/16 01:51 PM (7 years, 5 months ago)

Thanks dude, good luck!


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