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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Bumholio]
#25589683 - 11/03/18 05:39 PM (5 years, 2 months ago) |
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Yes and no, I only managed about another week with tea vs no tea (open to air). It has a few other side effects. It makes the grains more acidic and also harder, and can attract various lignicolous molds.
It does reduce yeast and common bacteria numbers, but not molds.
Due to the modification, growth 'may' be weak, depending on your mycelium.
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
Last seen: 16 hours, 52 minutes
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Re: Cultivation General Discussion [Re: Ferather]
#25590805 - 11/04/18 05:27 AM (5 years, 2 months ago) |
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Antibiotic agar works for me. Its a good tool and I dont really see a ton of downsides at this point. My technique always needs improvement but I also manage to pull good weight for years at a time with no breaks.
Again the question was just whether folks thought that leaving a donor jar of LI in front of a flow hood uncovered during innoculations might be a place that contams can get in.
If I thought it was bacteria on my plates I'd use more antibiotic. I ran regular MEA for a 1.5 years before going to antibiotic and I rarely saw any bacteria except maybe on a germ plate.
Thanks again.
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Shadowboxing the apocalypse and wandering the land
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Crispykoot]
#25590835 - 11/04/18 06:08 AM (5 years, 2 months ago) |
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The same for me, when I started with T-Gel. I examined a few vectors when I designed it.
The first vector, was to not sterilize it, simple boiling water and a 45 second microwave. Nothing grew even after 4 weeks (open air assembly, lid on after). Next, leave the plate fully exposed to see what happens. Nothing after 4 weeks, unless modified (higher pH), which germinated 3 different mycelium. Next transfer of yeast and common bacteria, was a pass, transfer of archaea was a negative (it's decays phenols) and it grew out.
Conclusion, containing no sugar and starch reduces contamination very largely, only organisms capable of decaying phenols will grow.
Note: Archaea does not produce spores, it was transferred and obtained from seaweed.
All tests where done without the optional grain flour additive.
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Cultivation General Discussion [Re: Crispykoot]
#25590933 - 11/04/18 07:21 AM (5 years, 2 months ago) |
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Quote:
Crispykoot said: Antibiotic agar works for me. Its a good tool and I dont really see a ton of downsides at this point. My technique always needs improvement but I also manage to pull good weight for years at a time with no breaks.
Again the question was just whether folks thought that leaving a donor jar of LI in front of a flow hood uncovered during innoculations might be a place that contams can get in.
If I thought it was bacteria on my plates I'd use more antibiotic. I ran regular MEA for a 1.5 years before going to antibiotic and I rarely saw any bacteria except maybe on a germ plate.
Thanks again.
antibacterial agar only prevents bacteria from germinating. there could be endospores all over your plates when you go to expand it in LI, which will then germinate when you put it on grains. thats why I never recommend anyone use antibacterial agar. learn to clean your cultures the right way, and not use a crutch like antibacterial agar.
the point of agar is for anything that could potentially germinate on your grains to germinate on the agar instead, so you can transfer away from it. so you are kind of missing the point if you use antibacterial agar as your daily driver. you want the bacteria to germinate on the agar, if its going to germinate anywhere. go back to MEA.
Edited by Munchauzen (11/04/18 07:28 AM)
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ithikuss
Level 4 Mage



Registered: 08/13/18
Posts: 650
Loc: Nightosphere
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Re: Cultivation General Discussion [Re: Munchauzen]
#25591005 - 11/04/18 07:59 AM (5 years, 2 months ago) |
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Quote:
Munchauzen said:
Quote:
Crispykoot said: Antibiotic agar works for me. Its a good tool and I dont really see a ton of downsides at this point. My technique always needs improvement but I also manage to pull good weight for years at a time with no breaks.
Again the question was just whether folks thought that leaving a donor jar of LI in front of a flow hood uncovered during innoculations might be a place that contams can get in.
If I thought it was bacteria on my plates I'd use more antibiotic. I ran regular MEA for a 1.5 years before going to antibiotic and I rarely saw any bacteria except maybe on a germ plate.
Thanks again.
antibacterial agar only prevents bacteria from germinating. there could be endospores all over your plates when you go to expand it in LI, which will then germinate when you put it on grains. thats why I never recommend anyone use antibacterial agar. learn to clean your cultures the right way, and not use a crutch like antibacterial agar.
the point of agar is for anything that could potentially germinate on your grains to germinate on the agar instead, so you can transfer away from it. so you are kind of missing the point if you use antibacterial agar as your daily driver. you want the bacteria to germinate on the agar, if its going to germinate anywhere. go back to MEA.
I've only been doing agar for a short time now, but this makes nothing but sense... Also, if your technique is half good and clean your area (sab or in front of a filter) you should have only initially dirty plates. I have done a good amount of transfers and only 2 plates get signs of contam, 2/20+ transfers so far... which I am grateful when they showed up before getting thrown to spawn.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: ithikuss]
#25591339 - 11/04/18 10:33 AM (5 years, 2 months ago) |
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@Munchauzen
Fair point, so now I have a few questions.
1) In your given scenario, but instead agar to grain, wouldn't the endospore need to be in contact with grain to grow? 2) Would a recipe that allows endospores to germinate, show themselves, and then stall, be beneficial? 3) If I took a side scraping with a sterilized tool, what are the chances I get endospores?
I am willing to research a recipe that allows for question 2 to take place.
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butterflyaway
Kaleidoscope Queen



Registered: 10/26/18
Posts: 114
Loc:
Last seen: 3 years, 5 months
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Hey All,
I have searched these forums for an answer to this question and find varying thoughts of opinion. I’m hoping to get a singular thought if possible.
I’ve just harvested my first flush. I was ok, since I was kinda lost trying to get there but I did get 85 grams most of which were off of 2 of the 10 cakes I have.
All cakes pinned but some were tiny and haven’t really done anything yet. Some have opened up and I picked most of those.
So I’ve heard
Dunk them and roll them again pins and all for a second flush
Spray them for a second flush
Pick all the pins off and dunk and roll.
Which is correct.
Your help is appreciated.
Thanks!
-------------------- There is a better way. Finding the way — well that's the journey
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Bumholio
What's the craic



Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
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I would pick them, dunk 8 hrs, no roll, back in to fc. Continue til it don't fruit no more.
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 "great things may come to those who wait, but only what's left by those who hustle"
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 11 hours
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I don't think any more than an hour will help anything
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Nimph
Im Unclecaptainblue!!!!

Registered: 08/12/18
Posts: 1,605
Loc: The dirty
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Dunk over night your in no rush anyways.
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  Hunters save your exotic prints to trade! https://www.shroomery.org/forums/showflat.php/Number/25617539
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Smooby2
Learner


Registered: 07/13/18
Posts: 143
Last seen: 2 years, 11 months
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Re: Small Pins [Re: Nimph]
#25592069 - 11/04/18 04:51 PM (5 years, 2 months ago) |
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Anyone on here have experience building a flow hood? I have a few questions.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Quote:
IntergalacticSpore said: Lol I'm not trying to shit on you or anything but if you watched further you would hear him say "laptop or phone". You were a second or two too short on the patience.
No, I heard him mention the phone. I'm saying he called it a "labtop" like a moron would. It's a laptop.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Damn stare those tubs in your sig are dope holmes ... Man I miss the life haha
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: Boogieman47]
#25592520 - 11/04/18 08:11 PM (5 years, 2 months ago) |
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Thanks, brother. You'll be back soon enough. Your grows were always more impressive than mine, ha.
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Buck513

Registered: 04/17/14
Posts: 5,682
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”Labtop.”
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Cultivation General Discussion [Re: Buck513]
#25593761 - 11/05/18 11:35 AM (5 years, 2 months ago) |
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Thought this was pretty cool. Had a clone plate contaminated with green mold but I had let it sit for a couple days. The P. Caerulescens mycelium is fighting back against the mold.
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butterflyaway
Kaleidoscope Queen



Registered: 10/26/18
Posts: 114
Loc:
Last seen: 3 years, 5 months
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Quote:
Bumholio said: I would pick them, dunk 8 hrs, no roll, back in to fc. Continue til it don't fruit no more.
Is there any harm in not being able to pick all the small pins that did not mature. If they are not opened up? I could probably get them with a tweezer or something just don't want to destroy the mycelium. And, I don't want to stunt the second flush, if that is a possibility.
Still figuring this thing out.
Thanks for the help!
-------------------- There is a better way. Finding the way — well that's the journey
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Bumholio
What's the craic



Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
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Nah, they'll be grand, you can brush them off if you want or just leave them and myc will grow over them, cakes are pretty resilient, I've had one that I thought was fucked didn't think it was going to flush at all it's been in the fc for over 2 weeks with very little happening. Barely misted it, totally neglected it and it started pinning the other day.
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 "great things may come to those who wait, but only what's left by those who hustle"
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butterflyaway
Kaleidoscope Queen



Registered: 10/26/18
Posts: 114
Loc:
Last seen: 3 years, 5 months
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Quote:
Bumholio said: Nah, they'll be grand, you can brush them off if you want or just leave them and myc will grow over them, cakes are pretty resilient, I've had one that I thought was fucked didn't think it was going to flush at all it's been in the fc for over 2 weeks with very little happening. Barely misted it, totally neglected it and it started pinning the other day.
Right on! Thanks
-------------------- There is a better way. Finding the way — well that's the journey
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Quote:
butterflyaway said:
Quote:
Bumholio said: I would pick them, dunk 8 hrs, no roll, back in to fc. Continue til it don't fruit no more.
Is there any harm in not being able to pick all the small pins that did not mature. If they are not opened up? I could probably get them with a tweezer or something just don't want to destroy the mycelium. And, I don't want to stunt the second flush, if that is a possibility.
Still figuring this thing out.
Thanks for the help!
8 hours is not enough to hydrate the cake fully. 12-24h is better
-------------------- Cakes inside Water Tub
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