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tryingtotrip
Stranger

Registered: 09/26/18
Posts: 57
Last seen: 5 years, 1 month
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Re: Cultivation General Discussion [Re: bodhisatta]
#25581804 - 10/31/18 01:26 PM (5 years, 2 months ago) |
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"Glucose oxidase is practically inactive in full strength honey. It becomes active to form hydrogen peroxide only when the honey is diluted. One explanation for this is that the activity of the enzyme is suppressed by the low pH in ripened honey. The enzyme has an optimum pH of 6.1, with a good activity from pH 5.5 to pH 8, but the activity drops off sharply below pH 5.5 to near zero at pH 4. Another possible explanation is that that enzymes need a sufficiently high level of free water to be active, and in undiluted honey the water that is present is almost all bound up on the sugar molecules."
I would have to agree that when it is diluted as much as the honey LC it has less effect but not diminished. Also, like I said it depends on the quality of the honey. Many can get away with cheap honey but probably not the honey he is using.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: tryingtotrip]
#25581890 - 10/31/18 01:55 PM (5 years, 2 months ago) |
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Buy pure honey, add a shot glass of water 30mL or 1oz to 8oz/250mL of the more pure organic A+++ quality honey you can find. And then tell me what happened to it after a week.
We use honey at 1-4% where it becomes a food for all microorganisms.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: bodhisatta]
#25581970 - 10/31/18 02:27 PM (5 years, 2 months ago) |
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I agree with bod, it will be the amount of sugars that will preserve honey, and why it can preserve various materials. High concentrations of most materials will be preservative, such as lots of salt (which was also used).
I know honey works (sometimes) and pure sucrose does not, so here is the difference, nutrients.
Both honey and sucrose are a combination of glucose-fructose as the carbon source. However, honey contains nitrogen, vitamins* and generally more nutrients.
Honey nutrients | Sucrose (white sugar) nutrients.
Many inhibitory effects can be overcome when there is enough potential in the organism. And also what bod has said, any inhibitory materials would be diluted in water.
Based on the nutritional analysis, inhibitory effects would be trace.
* Vitamins can also contain nitrogen.
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tryingtotrip
Stranger

Registered: 09/26/18
Posts: 57
Last seen: 5 years, 1 month
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Re: Cultivation General Discussion [Re: bodhisatta]
#25582036 - 10/31/18 02:59 PM (5 years, 2 months ago) |
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All I’m saying is it has more anti properties than table sugar and it is ignorant to say it doesn’t. This isn’t a binary answer and honey is extremely multifactorial in its properties like polyphenols and what ever properties the pollen of the certain flower had. It does have more nutrients but is more problematic (in theory ) than just mea or simple sugar
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tryingtotrip
Stranger

Registered: 09/26/18
Posts: 57
Last seen: 5 years, 1 month
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Re: Cultivation General Discussion [Re: Ferather]
#25582068 - 10/31/18 03:17 PM (5 years, 2 months ago) |
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But if the organism is futile and can’t overcome the anti fungal properties?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: tryingtotrip]
#25582122 - 10/31/18 03:38 PM (5 years, 2 months ago) |
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There's too much sugar, various inhibitory effects can be overcome as more carbon-other becomes available. I remember a test I read, where trich grew out vs inhibitory effects, when sugar was added.
Given that honey is around 82% sugar, I doubt it will have any real effect.
It's more likely the honey you used has a nutrient deficiency, if not treated (organic). Even grains vary, given the small amounts present, honey could be bland.
Also note, adding carbon to carbon upsets the nitrogen ratio (more carbon than before).
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Cultivation General Discussion [Re: Ferather]
#25582843 - 10/31/18 09:24 PM (5 years, 2 months ago) |
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Boogie is back I am free well kinda I wont be on much but missed you all and will be on as soon as I can
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Cultivation General Discussion [Re: Boogieman47]
#25582973 - 10/31/18 10:47 PM (5 years, 2 months ago) |
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Whats the latest craze ?? Anyone figure out how to make train sterilize itself haha
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: Boogieman47]
#25583208 - 11/01/18 01:58 AM (5 years, 2 months ago) |
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Could it be possible that 100% mycelium growbox companies would intentionally screw with the genetics of shrooms to make them weak at colonizing or very picky?
-------------------- One day I will evolve from agar mushroom grower
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 12 hours
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Re: Cultivation General Discussion [Re: Boogieman47] 1
#25583249 - 11/01/18 02:19 AM (5 years, 2 months ago) |
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Quote:
IntergalacticSpore said: Could it be possible that 100% mycelium growbox companies would intentionally screw with the genetics of shrooms to make them weak at colonizing or very picky?
why would they do that when they can just be lazy and use shitty technique?
Quote:
Boogieman47 said: Whats the latest craze ?? Anyone figure out how to make train sterilize itself haha
missed the fuck out of you boog! did you miss the poke or pasty's ezlc
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25583286 - 11/01/18 02:53 AM (5 years, 2 months ago) |
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Quote:
tryptkaloids said: why would they do that when they can just be lazy and use shitty technique?
So they can keep you buying because you can't figure it out. I don't know I'm just looking for reasons to justify my enormous failures. I might be just completely incapable of seeing my bacterial contams. I'm very close to giving up on these mckennais I started with. Shit grows on agar fine but when I put it on rye grain it just spreads 1 centimeter until it exhausts whatever is in the agar and then stops moving.
The only substrate that is colonizing is the overly wet grain that I mixed with verm but that one is bacterial for sure because it's colonizing so slowly. It still hasn't fully colonized in 30-ish days, I'm not too shocked since that was inoculated with agar I made with clone. This last A2G was tier 3 agar though, the best 2 petri dishes out of 12 had really uniform growth no rhizo and no uncolonized spots after it finished colonizing agar 3 days later. Is sterilization the only reason why grain is harder? Because my PC is 15 PSI and I did 2 hours in it.
I might unironically start growing shrooms on petri dishes, 4 out of 7 of my first attempt to clone clone to agar pinned and had 1-4 fruiting bodies each. Could give BRF PF tek a try I guess.
-------------------- One day I will evolve from agar mushroom grower
Edited by IntergalacticSpore (11/01/18 03:10 AM)
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: Boogieman47] 2
#25583292 - 11/01/18 03:05 AM (5 years, 2 months ago) |
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Quote:
Boogieman47 said: Whats the latest craze ?? Anyone figure out how to make train sterilize itself haha
The Josex Poke is pretty crazy, people are doing unmodified lids often, Micropore tape has replaced Polyfil for many people in their tubs, and MudaFuka unfortunately passed away recently. Glad to see you back, brother. I hope you're as good as you can be.
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van hatton
Still a noob


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Boogieman47]
#25583306 - 11/01/18 03:34 AM (5 years, 2 months ago) |
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Quote:
Boogieman47 said: Boogie is back I am free well kinda I wont be on much but missed you all and will be on as soon as I can
Super glad to see u dude. How'd u eat?
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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ManifoldPrime
Per Ardua Ad Astra



Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 1 month
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Re: Cultivation General Discussion [Re: van hatton]
#25583378 - 11/01/18 05:04 AM (5 years, 2 months ago) |
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How much spawn would I need roughly for a 25l mono ? I dont normally do monos, and I got a couple jars of spawn ready but I wanna know the minimum I could get away with. Say two 1litre jars worth (2/3rds full) to 2l Coir? The tub is 40cm X 27 cm, so if I want bare minimum 3cm of sub depth I'd need around 3,2 l of sub. Sound right?
-------------------- My Magnum Opus:Thai Variety Comparison Please correct me if I say something wrong! | Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |
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teladi
FUNKSOULBROTHER


Registered: 06/27/17
Posts: 1,189
Loc: South Africa
Last seen: 5 months, 22 days
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I do 14 litres of sub in my 42 litre monos.
Is 3cm enough? I'd increasing your substrate depth by either dub tubbing, or using a shopping bag to cover them. You could do spawn runs with the lids on for both options.
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coltbord
Stranger


Registered: 10/31/18
Posts: 5
Last seen: 4 years, 10 months
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Re: Cultivation General Discussion [Re: teladi]
#25583454 - 11/01/18 06:42 AM (5 years, 2 months ago) |
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Hi all wonderful shroomers 
Can someone helpe me confirm that this coco brick is the right kind of coco coir to use for bulk spawn in a monotub?
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Bumholio
What's the craic



Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
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Re: Cultivation General Discussion [Re: coltbord]
#25583458 - 11/01/18 06:48 AM (5 years, 2 months ago) |
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You can get it cheaper from pets at home. It's like £3 for 650g blocks, if you order online and collect in store you can get 4 for £9
http://www.petsathome.com/shop/en/pets/coco-coir-fibre-brick-650g
Edited by Bumholio (11/01/18 06:49 AM)
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: coltbord]
#25583459 - 11/01/18 06:48 AM (5 years, 2 months ago) |
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That coir is for weed and has trichoderma added, which we don't want. So you'll need to sterilize it for use in mycology. And it's also expensive. Get coir used for reptile bedding. It doesn't have trichoderma added and doesn't need sterilization.
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
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Quote:
stareatclouds said: That coir is for weed and has trichoderma added, which we don't want. So you'll need to sterilize it for use in mycology. And it's also expensive. Get coir used for reptile bedding. It doesn't have trichoderma added and doesn't need sterilization.
How do you find out which has trich added?
-------------------- One day I will evolve from agar mushroom grower
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Cultivation General Discussion [Re: stareatclouds] 1
#25583474 - 11/01/18 07:03 AM (5 years, 2 months ago) |
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Quote:
stareatclouds said: Get coir used for reptile bedding. It doesn't have trichoderma added and doesn't need sterilization.
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