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DreamReality
MycoPilot



Registered: 03/31/09
Posts: 477
Last seen: 3 months, 13 days
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Quote:
IntergalacticSpore said:
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DreamReality said:
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IntergalacticSpore said: 2 days ago I inoculated 12 petri dishes I had the bright idea of substituting some of the rye flour I've been using with honey.
Doesn't sound promising if there's been 2 days of no growth.
Thing is, nothing is growing,the agar pieces that I put on top just got white from all sides and refused to touch the "lava" what the hell. This is my 5th transfer to agar, started with 7 and did 12 every round after with fairly okay rate. This time not even one is showing any growth. It's like as if I swapped distilled water for agar with 70% prof alcohol lmao. I'd guess it's bacterial if it was one or two but this is 12 I poured and I cooked that agar in PC for 90 minutes at 15 PSI, poured it in SAB so theres no way every one of them is contaminated.
This is so spooky. 
It's probably your agar recipe. The cubes must not be a fan of it
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: DreamReality]
#25579436 - 10/30/18 06:36 PM (5 years, 2 months ago) |
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Quote:
DreamReality said: It's probably your agar recipe. The cubes must not be a fan of it 
Yeah that's the conclusion I came to as well. Even mold don't like it too much judging by how the mold spot isn't doing much at all 1 day after it became visible.
This is intriguing failure.
-------------------- One day I will evolve from agar mushroom grower
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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90 minutes is a loooong time for agar, I do 20 minutes with 0 issues, maybe you caramelized the sugars.
Got some jars in the PC, my PESA LC has been ready for a minute lol.
 It's about time I get something besides truffles to grain!
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: 00Burnout]
#25579525 - 10/30/18 07:06 PM (5 years, 2 months ago) |
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Quote:
00Burnout said: 90 minutes is a loooong time for agar, I do 20 minutes with 0 issues, maybe you caramelized the sugars.
Got some jars in the PC, my PESA LC has been ready for a minute lol.
 It's about time I get something besides truffles to grain!
Hmm that could be possible, it still has 0.7% rye flour inside though, that should make it possible to grow on agar right?
-------------------- One day I will evolve from agar mushroom grower
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 9 hours
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Quote:
IntergalacticSpore said:
Quote:
DreamReality said: It's probably your agar recipe. The cubes must not be a fan of it 
Yeah that's the conclusion I came to as well. Even mold don't like it too much judging by how the mold spot isn't doing much at all 1 day after it became visible.
This is intriguing failure.
There is no failure. Only success and lessons
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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Quote:
IntergalacticSpore said:
Quote:
00Burnout said: 90 minutes is a loooong time for agar, I do 20 minutes with 0 issues, maybe you caramelized the sugars.
Got some jars in the PC, my PESA LC has been ready for a minute lol.
 It's about time I get something besides truffles to grain!
Hmm that could be possible, it still has 0.7% rye flour inside though, that should make it possible to grow on agar right?
It should, but how much honey did you use? If your agar is super nutritious it could take a long ass time to colonize because of the availability of nutes. The idea is to have just enough nutrition for the mycelium to need to actively look for food without weakening it. I use approx 2% malt extract in my agar
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: 00Burnout]
#25579678 - 10/30/18 07:57 PM (5 years, 2 months ago) |
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Quote:
00Burnout said: It should, but how much honey did you use? If your agar is super nutritious it could take a long ass time to colonize because of the availability of nutes. The idea is to have just enough nutrition for the mycelium to need to actively look for food without weakening it. I use approx 2% malt extract in my agar
Its 2-2.1% it is simple sugar though.
-------------------- One day I will evolve from agar mushroom grower
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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Seems kinda high with the rye flour in there, been a long ass time since I looked into it but I think you want to keep overall sugar below 2%. I could be wrong and I'm sure someone will chime in and correct me if I am lol
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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teladi
FUNKSOULBROTHER


Registered: 06/27/17
Posts: 1,189
Loc: South Africa
Last seen: 5 months, 22 days
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Re: Cultivation General Discussion [Re: 00Burnout]
#25580389 - 10/31/18 12:42 AM (5 years, 2 months ago) |
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I just found three boxes of prepped jars in my store that I forgot about. Foil included. Huzzah! I can continue to ignore the pile of used jars for a few more days.
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: 00Burnout]
#25580523 - 10/31/18 02:01 AM (5 years, 2 months ago) |
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Quote:
00Burnout said: Seems kinda high with the rye flour in there, been a long ass time since I looked into it but I think you want to keep overall sugar below 2%. I could be wrong and I'm sure someone will chime in and correct me if I am lol
Man I did 7% rye flour first time I did agar and it was fine. Meanwhile a 2% mix of honey and rye flour results in this after 3 days. What is dis. Lmao.

 
-------------------- One day I will evolve from agar mushroom grower
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Nimph
Im Unclecaptainblue!!!!

Registered: 08/12/18
Posts: 1,605
Loc: The dirty
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Did umakd the flour yourself or by it from s store?store stuff dont work causd preservatives.
Use 1 taple spoon in boiling water 250ml and 2 agar after the water changes color. You cant throw haft a handful in the grain flour boil for darker agar an different nutes for your myc.
That recipe work great
--------------------
  Hunters save your exotic prints to trade! https://www.shroomery.org/forums/showflat.php/Number/25617539
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: Nimph]
#25580577 - 10/31/18 03:02 AM (5 years, 2 months ago) |
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Quote:
Nimph said: Did umakd the flour yourself or by it from s store?store stuff dont work causd preservatives.
Use 1 taple spoon in boiling water 250ml and 2 agar after the water changes color. You cant throw haft a handful in the grain flour boil for darker agar an different nutes for your myc.
That recipe work great
That's the thing I've used this same flour 4 times already and it worked perfectly.
-------------------- One day I will evolve from agar mushroom grower
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 9 hours
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Quote:
IntergalacticSpore said:
Quote:
00Burnout said: Seems kinda high with the rye flour in there, been a long ass time since I looked into it but I think you want to keep overall sugar below 2%. I could be wrong and I'm sure someone will chime in and correct me if I am lol
Man I did 7% rye flour first time I did agar and it was fine. Meanwhile a 2% mix of honey and rye flour results in this after 3 days. What is dis. Lmao.

  
too much honey would be my guess... honey has antifungal properties
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25580983 - 10/31/18 08:23 AM (5 years, 2 months ago) |
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Quote:
tryptkaloids said: too much honey would be my guess... honey has antifungal properties
Time to start sterilizing my scalpel with honey
-------------------- One day I will evolve from agar mushroom grower
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tryingtotrip
Stranger

Registered: 09/26/18
Posts: 57
Last seen: 5 years, 1 month
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It’s definitely the anti fungal properties of honey that is inhibiting growth. It also depends on the type of honey you’re using. If it’s a cheap brand that has a mix of simple sugar/honey then you could have better odds but if it’s true honey then have fun trying to grow anything on those dishes. Why use honey? Just add a little cane sugar or boil oats and use the water left over
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Tookitooki
Mycological Fabricator



Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Cultivation General Discussion [Re: tryingtotrip]
#25581287 - 10/31/18 10:13 AM (5 years, 2 months ago) |
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Edited by Tookitooki (10/31/18 10:15 AM)
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Tookitooki
Mycological Fabricator



Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Cultivation General Discussion [Re: Tookitooki]
#25581321 - 10/31/18 10:21 AM (5 years, 2 months ago) |
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If I had to take a guess, maybe to many nutes. I ran a batch of agar using concentrated grain soak water. Half no additional nutes, half got 1% Dex. Both grew like shit. Went from great looking cultures, to WTF. Growth was very very slow and super thick. Last night I finally transferred those cultures, from those plates, back to MEA. Just to see what the myc does. Also dropped to jars, for science.
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: Tookitooki]
#25581393 - 10/31/18 10:44 AM (5 years, 2 months ago) |
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Quote:
Tookitooki said: If honey is an issue, how does honey LC work so well.
https://www.shroomery.org/forums/showflat.php/Number/22929639#22929639
That's what I thought when I made the agar. I also made honey LC, that one ain't growing either lol. My last petris are made with 2% light DME and 1.5% agar only. Just inoculated them today but I had to reuse petris for source that I opened twice already so there's almost certain to be some contams.
Oh as for honey, its some really healthy honey I get directly from the beekeeper that I know is legit. If natural honey is stronger and honey by itself is antimicrobial nothing will grow on that thing hahaha.
 I welcome the learning experience.
-------------------- One day I will evolve from agar mushroom grower
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tryingtotrip
Stranger

Registered: 09/26/18
Posts: 57
Last seen: 5 years, 1 month
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Re: Cultivation General Discussion [Re: Tookitooki]
#25581762 - 10/31/18 01:12 PM (5 years, 2 months ago) |
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Quote:
Tookitooki said: If honey is an issue, how does honey LC work so well.
https://www.shroomery.org/forums/showflat.php/Number/22929639#22929639
Probably using fake or cheap honey like I said in my post. Why do you think honey never goes bad and was actually used to preserve foods in the past.
"Not only is honey antibacterial, but it is antiviral and antifungal. These properties alone make it more powerful than conventional antibiotics. Honey can target undetected fungal conditions that may be at the root cause of perpetual illness.
Meschwitz said that her team of researchers has been measuring the level of antioxidant activity of honey. "We have separated and identified the various antioxidant polyphenol compounds. In our antibacterial studies, we have been testing honey's activity against E. coli, Staphylococcus aureus and Pseudomonas aeruginosa, among others."
While many commercial brands of honey are filtered and fake, the best place to look for delicious, medicinal, and unfiltered raw honey is at local honeybee farms."
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: tryingtotrip]
#25581766 - 10/31/18 01:13 PM (5 years, 2 months ago) |
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Honey isn't anti anything once diluted. The only reason its antibacterial and antifungal is because its so hygroscopic when in its natural state. It is like a desiccant.
A jar of sugar is anti bacterial and anti fungal too so is corn syrup if sufficiently thick enough (low water content).... Lol
Add a shot glass of water to jar of honey stir it up and it will mold and go bacterial like mad in a week.
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