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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 9 hours
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Re: Cultivation General Discussion [Re: JHOVA]
#25572614 - 10/27/18 09:17 PM (5 years, 3 months ago) |
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Idk what's up with me today, but when i was in there it was too much of a shitshow to trust it without iso.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25572615 - 10/27/18 09:18 PM (5 years, 3 months ago) |
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Any worse than my video where it starred michael j fox as the lead charactor?
Got any vids
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 9 hours
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Re: Cultivation General Discussion [Re: mushboy]
#25572621 - 10/27/18 09:20 PM (5 years, 3 months ago) |
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Naw, that was the last thing on my mind. I gotta stop dosing before I get things done
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25572634 - 10/27/18 09:25 PM (5 years, 3 months ago) |
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I was rusty the other night myself. It's been 6months
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,672
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Re: Cultivation General Discussion [Re: mushboy]
#25573129 - 10/28/18 02:17 AM (5 years, 3 months ago) |
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Quote:
mushboy said: I was rusty the other night myself. It's been 6months
I'm a seasonal grower, don't like to run the dehydrator and the AC. So I can relate. Just did my 3rd round of agar work this season and seem worse each time. Way out of practice.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25573243 - 10/28/18 05:53 AM (5 years, 3 months ago) |
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Quote:
tryptkaloids said: I honestly just want to grow enough cubes to keep my depression at bay. I can do that. Im just honing it to do it cheaper
Ok, I thought your where implying you where being aimless and just posting kinda randomly (and in some cases being a bit rude).
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: Ferather]
#25573251 - 10/28/18 06:04 AM (5 years, 3 months ago) |
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Can you transfer a culture on agar or some other substrate too many times?
-------------------- One day I will evolve from agar mushroom grower
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gizmodo
Woodland Creature




Registered: 06/21/18
Posts: 2,064
Loc: Behind You
Last seen: 2 years, 8 months
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The further you transfer away from the origin the weaker it becomes I believe. That one of the reasons why keeping slants is a good idea, it allows you to keep cell divisions down by staying close to origin.
Edited by gizmodo (10/28/18 06:16 AM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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A master on agar will need expanding eventually, it's suggested to variate the media, this should encourage material detection and various enzymes. If you stick to the same recipe when expanding the master, it could end up being picky after transfer, or generating senescence.
There are various factors that will cause cellular senescence, DNA damage, and spores will be needed eventually.
An example is malt extract, it's soluble (not suggested long term), and made from grain + enzymes. The mycelium should secrete it's own enzymes, so I suggest flour or grain water agar.
You can also try a more complex sugar source such as cellulose (egg carton).
In most cases you use sterilized, cut down, toothpicks.
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DreamReality
MycoPilot



Registered: 03/31/09
Posts: 477
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Re: Cultivation General Discussion [Re: Ferather]
#25573279 - 10/28/18 06:41 AM (5 years, 3 months ago) |
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I believe senescence is something you obtain after like 120+ transfers. Plus they're cubes, they don't mind eating ''chicken'' for dinner every night.
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gizmodo
Woodland Creature




Registered: 06/21/18
Posts: 2,064
Loc: Behind You
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Re: Cultivation General Discussion [Re: DreamReality]
#25573281 - 10/28/18 06:43 AM (5 years, 3 months ago) |
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It is usually much less than 120.
-------------------- One must be mindful to maintain their balance on the slippery earth. Bod's Comprehensive Agar Resource Gizmodo's Market Stall Say No To Grow Kits
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DreamReality
MycoPilot



Registered: 03/31/09
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Re: Cultivation General Discussion [Re: gizmodo]
#25573290 - 10/28/18 06:48 AM (5 years, 3 months ago) |
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Quote:
bodhisatta said: Why Some Fungi Senesce and Others Do Not An Evolutionary Perspective on Fungal Senescence
Organisms with a ‘unitary body plan’ – such as many mobile animals – have a determinate structure composed of strict numbers of body parts, specialised organs and a separate germ line. Unitary organisms are arguably all subject to the process of senescence, resulting in death even under protected idealised conditions. Organisms with ‘modular body plans’, however – including plants, fungi and (sessile) animals such as hydroids and bryozoans – seem to escape this process and are potentially immortal. Modular organisms are composed of multiple genetically identical vegetative modules that may remain attached or become separated to form physiologically independent clones. Here all cells are in principle totipotent and capable of expansion and reproduction, so death of parts of a modular organism does not necessarily cause the death of the whole organism (Figure 17.1). In plant biology these modules are generally referred to as ‘ramets’. The ensemble of ramets that makes up a single genetic entity is generally referred to as a ‘genet’. The body plan of an organism has major implications for the wayselection can act. In modular organisms, selection rarely acts directly on the genet: the unit of selection is usually the ramet. Fungi are considered to be modular organisms with no clear distinction of a germ line. With the expansion of the mycelium, chances for reproduction are expected to increase,and each unit under favourable circumstances may produce offspring (Figure 17.1B, right). Fungi with such modular body plans are expected to be long-lived, as most fungi indeed seem to be. However, some fungi exist that do senesce, and their growth often seems to be limited by space or time. For these fungi, we consider the term ‘pseudounitary’ (Figure 17.1B, centre), as life history details and ecological conditions constrain the size of the soma and the opportunities for reproduction. In this chapter we discuss why fungi are usually long-lived and why there are exceptions to this. We predict life history traits and ecological conditions that favour the evolution of fungal senescence. Finally, we discuss the proximate mechanisms of fungal senescence in the light of this evolutionary context.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: DreamReality]
#25573299 - 10/28/18 06:53 AM (5 years, 3 months ago) |
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Here is another factor, and test, I can feed mycelium with 12%+ sugars, however on a complex media which is nutritional (macro-micro nutrients). The mycelium grew out vigorously rather than died, this is because the sugars where "spendable", which I will try to explain.
Enzymes, cells and various materials are composed of C-H-O, C-H-N-O plus variations, sucrose is 99.9% C-H-O. Due to ample macro-micro nutrient uptake-availability, the mycelium could spend the sugars.
The sugars where used along with the nutrients to produce cells, enzymes and so on.
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Here is my T-Gel, plain (no added flour), sorry for the image quality, I had temperature fluctuation issues. The first two are completely plain, the second two are with added macro-micro nutrients.
With added nutrients, the carbon present was more "spendable" as mentioned.


Tarragon oyster (lignicolous).
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@DreamReality
That's a nice quote, I have revived 2 year old spent, dried out mycelial tissue, a few times now.
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Edit: Add more data and illustrations (although with phenols, not sugars).
Edited by Ferather (10/28/18 07:16 AM)
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
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Re: Cultivation General Discussion [Re: sh4d0ws]
#25573336 - 10/28/18 07:16 AM (5 years, 3 months ago) |
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Quote:
sh4d0ws said: Anyone have suggestions for buying bulk rye or wheat in Ontario, Canada?
I know some farm feed stores will carry it but I've yet to find one. I have had success with finding wheat kernel at bulk barn but it is quite expensive.
Try getting "Fall Rye" seed at the farm feed store. People use it as a cover crop in the garden.Thats all I use. Bakery supply places will cary whole grains.
--------------------

Shadowboxing the apocalypse and wandering the land
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ManifoldPrime
Per Ardua Ad Astra



Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 1 month
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25573537 - 10/28/18 09:17 AM (5 years, 3 months ago) |
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Quote:
tryptkaloids said: Well, i made 6 qts and 6 pints yesterday. Went to inoculate them and it's like ive never seen a sab. I got 3 done and gave up. Today's not the day. I probably just wasted about 5 bux of grain. Whar would you do with it?
Trypt, dude, That was me like last week, It happens, you can overthink and mentally build up a myco task like SAB work until it seems monumental or impossible to do that day. Yesterday was the first time I threw out uninoculated grain that was left over from that sesh. Sometimes you feel like you're just fucking about imitating actual mycologists/biologists and every failure starts compounding a sense of personal failing.
Then you see a couple pins and suddenly it all seems worthwhile
-------------------- My Magnum Opus:Thai Variety Comparison Please correct me if I say something wrong! | Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Crispykoot]
#25573552 - 10/28/18 09:20 AM (5 years, 3 months ago) |
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Quote:
Crispykoot said:
Quote:
sh4d0ws said: Anyone have suggestions for buying bulk rye or wheat in Ontario, Canada?
I know some farm feed stores will carry it but I've yet to find one. I have had success with finding wheat kernel at bulk barn but it is quite expensive.
Try getting "Fall Rye" seed at the farm feed store. People use it as a cover crop in the garden.Thats all I use. Bakery supply places will cary whole grains.
Why spend more money for the same end result?
Rye berries from a specialty store or whole oats from TSC. 25kg' of whole oats costs a fraction of the price of anything else.
Edited by Stipe-n Cap (10/28/18 09:50 AM)
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#25573719 - 10/28/18 10:49 AM (5 years, 3 months ago) |
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oats are a pain to get down. they require way too much dry time for my liking. switching from animal food to people grade wheat was a major step towards clean spawn for me
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25573742 - 10/28/18 10:59 AM (5 years, 3 months ago) |
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Quote:
tryptkaloids said: oats are a pain to get down. they require way too much dry time for my liking. switching from animal food to people grade wheat was a major step towards clean spawn for me
To each their own, for me it was the opposite. The reduction in dry time doesn't justify the increased cost for me....but that's just me. I plan on making a wood framed screen drying rack with a box fan underneath, (seen it somewhere on the forum) this should significantly reduce drying time. (Which isn't really a problem to begin with, I just like projects)
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Wing
The Eye Tyrant



Registered: 04/25/11
Posts: 3,293
Last seen: 5 months, 11 days
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#25573809 - 10/28/18 11:40 AM (5 years, 3 months ago) |
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Quote:
p9hu7 said:
Quote:
tryptkaloids said: oats are a pain to get down. they require way too much dry time for my liking. switching from animal food to people grade wheat was a major step towards clean spawn for me
To each their own, for me it was the opposite. The reduction in dry time doesn't justify the increased cost for me....but that's just me. I plan on making a wood framed screen drying rack with a box fan underneath, (seen it somewhere on the forum) this should significantly reduce drying time. (Which isn't really a problem to begin with, I just like projects)
Dry time is easily shortened with a paper towel rub down I've tried fans, screens, heat, etc. Physically getting the moisture off is the fastest. Still needs to dry a slight bit more after though.
-------------------- My Old Grow Logs
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Wing] 1
#25573821 - 10/28/18 11:50 AM (5 years, 3 months ago) |
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Quote:
Wing said:
Quote:
p9hu7 said:
Quote:
tryptkaloids said: oats are a pain to get down. they require way too much dry time for my liking. switching from animal food to people grade wheat was a major step towards clean spawn for me
To each their own, for me it was the opposite. The reduction in dry time doesn't justify the increased cost for me....but that's just me. I plan on making a wood framed screen drying rack with a box fan underneath, (seen it somewhere on the forum) this should significantly reduce drying time. (Which isn't really a problem to begin with, I just like projects)
Dry time is easily shortened with a paper towel rub down I've tried fans, screens, heat, etc. Physically getting the moisture off is the fastest. Still needs to dry a slight bit more after though.
I'll grab something more sturdy than paper, maybe a dedicated shammy that I can wash and re-use.
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