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InvisibleTookitooki
Mycological Fabricator
 User Gallery


Registered: 07/28/16
Posts: 1,157
Loc: Nowhere Flag
Re: Cultivation General Discussion [Re: Brain Bulb]
    #25563076 - 10/24/18 11:25 AM (5 years, 3 months ago)

Quote:

Brain Bulb said:
Ok, so I need to freeze it just not in the glass jars, lol.

The reason they are so full is because they are half pint jars not pint.  As far as inoculation, I’m not sure yet.  Would an agar wedge work?  If not I’ll do a LI.

Thanks all!

BB




Ice cube trays are great as mushboy said.  I used cupcake pans(wife likes to make cupcakes a lot).  I got two, gallon bags of gw pucks.


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Offlineteladi
FUNKSOULBROTHER


Registered: 06/27/17
Posts: 1,189
Loc: South Africa Flag
Last seen: 5 months, 22 days
Re: Cultivation General Discussion [Re: Tookitooki]
    #25563329 - 10/24/18 12:47 PM (5 years, 3 months ago)

Quote:

Tookitooki said:
Ice cube trays are great as mushboy said.  I used cupcake pans(wife likes to make cupcakes a lot).  I got two, gallon bags of gw pucks.






These are my prepped LC bottles (250ml Duran/Schott). 50 ml of GW and glass shards to be thawed diluted to 250ml when needed. Polyfill lids. They take more space your pucks, but bottle is already done. Mini cupcake pans may work for my bottles - make pucks from those and slip them right through. 40ish ml per puck with those. I imagine your pucks are closer to 100ml?


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InvisibleComebackKid
Multispore Enthusiast
Male User Gallery


Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
Trusted Cultivator
Re: Cultivation General Discussion [Re: teladi]
    #25563440 - 10/24/18 01:27 PM (5 years, 3 months ago)

Hey guys I'm looking for a reputable grower who also has access to a microscope  and can take quality microscopic pictures of spores...

If you know anyone who fits the bill can you drop some names for me?


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleGerms
Space Force
 User Gallery

Registered: 06/26/11
Posts: 4,607
Loc: Texas
Trusted Cultivator
Re: Cultivation General Discussion [Re: ComebackKid]
    #25563455 - 10/24/18 01:31 PM (5 years, 3 months ago)

Quote:

ComebackKid said:
Hey guys I'm looking for a reputable grower who also has access to a microscope  and can take quality microscopic pictures of spores...

If you know anyone who fits the bill can you drop some names for me?



Bodhisatta and verum come to mind
Not sure if fishermansjc has a scope


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Invisiblevan hattonFacebookDiscord
Still a noob
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Registered: 11/23/14
Posts: 5,617
Loc: Michigan
Trusted Cultivator
Re: Cultivation General Discussion [Re: Germs]
    #25563477 - 10/24/18 01:39 PM (5 years, 3 months ago)

Pretty sure he does


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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InvisibleComebackKid
Multispore Enthusiast
Male User Gallery


Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
Trusted Cultivator
Re: Cultivation General Discussion [Re: van hatton]
    #25563502 - 10/24/18 01:49 PM (5 years, 3 months ago)

Sweet thanks!!


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineFishLevelMidnight
Aquaman
Male User Gallery


Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 25 days
Re: Cultivation General Discussion [Re: ComebackKid]
    #25563703 - 10/24/18 02:54 PM (5 years, 3 months ago)

:hug:

I’d love to take some pics if you want to send a sample of spores .

I can add scale bar and take measurements as well


--------------------





Trade List





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InvisibleZero Nowhere
Beer Drinker
Male


Registered: 01/29/18 Happy 6th Shroomiversary!
Posts: 1,471
Loc: Standing on the moon
Re: Cultivation General Discussion [Re: FishLevelMidnight]
    #25564144 - 10/24/18 05:00 PM (5 years, 3 months ago)

Does anyone want a wild armillaria mellea print?  I have a shit ton.


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OfflineBrain Bulb
Insane in the Membrane
 User Gallery


Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
Re: Cultivation General Discussion [Re: Zero Nowhere]
    #25565780 - 10/25/18 08:40 AM (5 years, 3 months ago)

Any of you had a transfer come out like this?  Makes me think of a symbiote, lol.


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OfflineLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,023
Last seen: 4 hours, 54 minutes
Re: Cultivation General Discussion [Re: Brain Bulb]
    #25565978 - 10/25/18 10:07 AM (5 years, 3 months ago)

My guess is mold is meshed in that culture... How many days since you transfered that wedge to the new plate? That actually looks like straight mold to me not mycelium


Edited by Leftfield420 (10/25/18 10:38 AM)


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 9 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: Leftfield420]
    #25566051 - 10/25/18 10:32 AM (5 years, 3 months ago)

:whathesaid: start over.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineBrain Bulb
Insane in the Membrane
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Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
Re: Cultivation General Discussion [Re: Leftfield420]
    #25566337 - 10/25/18 12:00 PM (5 years, 3 months ago)

Quote:

Leftfield420 said:
My guess is mold is meshed in that culture... How many days since you transfered that wedge to the new plate? That actually looks like straight mold to me not mycelium





It’s been about 3 days.  Posted it on Bod’s Q&A as well and he said it looks about right.  If it was coming from the bottom up I’d be more inclined to think it’s mold.  Idk, gonna let it incubate a little longer


--------------------


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OfflineLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,023
Last seen: 4 hours, 54 minutes
Re: Cultivation General Discussion [Re: Brain Bulb]
    #25566393 - 10/25/18 12:18 PM (5 years, 3 months ago)

Time is about right..did you sandwhich myc between agar? But still looks like something is riding along...


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 9 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: Brain Bulb]
    #25566397 - 10/25/18 12:19 PM (5 years, 3 months ago)

It looks like hell to clean. I'd drop more spores


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineBrain Bulb
Insane in the Membrane
 User Gallery


Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25566474 - 10/25/18 12:54 PM (5 years, 3 months ago)

Quote:

Leftfield420 said:
Time is about right..did you sandwhich myc between agar? But still looks like something is riding along...




Could be.  Just hard to tell for sure right now.  PCS are very resistant to mold and bacteria.  I’ve incoluted 3 plates direct from a wild print and none have shown contamination.  This was from a clone so it’s a little different.  I’ll give it some more time to incubate before I do anything.

Quote:

tryptkaloids said:
It looks like hell to clean. I'd drop more spores




I have other plates.  This was one of a few transfers from a wild specimen I cloned a few days ago.


--------------------


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OfflineIntergalacticSpore
Grows shrooms on agar
Male


Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
Re: Cultivation General Discussion [Re: Brain Bulb]
    #25569550 - 10/26/18 03:26 PM (5 years, 3 months ago)

Any european cultivators here? I'm looking at "affordable" pressure canner or large pressure cooker that can do 15 PSI and hold 7 jars. I already have a really good pressure cooker that does everything very well but it can only hold 3 jars, yesterday I spent 9 hours preparing 9 jars and I think it's time for a change.

I see a lot of pressure canners on ebay and amazon, the problem is, they are all coming from US. I pay 123$ that's okay 50$ more for shipping and 36$ for VAT... Turns the whole deal a bit sour.

Where do you european cultivators buy okay pressure cookers at reasonable prices?:shrug:


--------------------
One day I will evolve from agar mushroom grower :goat:


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Cultivation General Discussion [Re: IntergalacticSpore]
    #25569561 - 10/26/18 03:29 PM (5 years, 3 months ago)

they buy them at very unreasonable prices it seems from what I've heard


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InvisibleLizardWizard
GnomeGrower
I'm a teapot User Gallery


Folding@home Statistics
Registered: 01/07/15
Posts: 13,692
Loc: the parking lot
Re: Cultivation General Discussion [Re: IntergalacticSpore] * 1
    #25569593 - 10/26/18 03:45 PM (5 years, 3 months ago)

:wave:

If you want an affordable option for a sterilizer, these are what I use, though you do have to build a controller option for ease of use :shrug:

18L stainless steel dental autoclave, from china



or same one, but from within the EU

You might have to pay import charges when you import from china, and shipping will take longer.

IIRC I bought mine from a chinese company with a shipping dock in Germany, so the customer service is equally chinglish either way. I like that it's an electric unit, and with the controller I made for about 20 bucks I can run it pretty easily without much babysitting once it's up to pressure. I also like that it runs silent (aside from venting of course) and that it has a very sturdy feel to it. Gasket and vents are replaceable. Still adds up to around 210-220 for the whole thing though.


--------------------
The best things in life
can be smelled on one's fingers.


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OfflineIntergalacticSpore
Grows shrooms on agar
Male


Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
Re: Cultivation General Discussion [Re: bodhisatta]
    #25569597 - 10/26/18 03:47 PM (5 years, 3 months ago)

Quote:

bodhisatta said:
they buy them at very unreasonable prices it seems from what I've heard


Ouch...
This is what I'm looking at by the way.


--------------------
One day I will evolve from agar mushroom grower :goat:


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OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Cultivation General Discussion [Re: bodhisatta] * 1
    #25569606 - 10/26/18 03:50 PM (5 years, 3 months ago)



--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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