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LizardWizard
GnomeGrower




Registered: 01/07/15
Posts: 13,692
Loc: the parking lot
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Re: Marmite is a rubbish agar additive [Re: JHOVA]
#25555801 - 10/21/18 03:34 PM (5 years, 3 months ago) |
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What a wonderful world, ain't it?
-------------------- The best things in life can be smelled on one's fingers.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Marmite is a rubbish agar additive [Re: JHOVA]
#25555813 - 10/21/18 03:38 PM (5 years, 3 months ago) |
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Think I know why I was getting mold/bacteria in my last batch of agar. Was in a hurry when it was coming down from PC so I may have helped it come down a little faster That or switching to spraying my SAB with soapy water rather than ISO mix. Either way things seem to be back on track.
On another note I found some more Caerulescens pinners starting in my yard a couple days ago but the temp has dropped and there’s a frost warning tonight. Is it worth it to take a clone from a pinner?
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
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Cultivation General Discussion [Re: Brain Bulb]
#25555840 - 10/21/18 03:46 PM (5 years, 3 months ago) |
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Thread title should not be fucked with
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: JHOVA]
#25555849 - 10/21/18 03:49 PM (5 years, 3 months ago) |
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Agreed. People have asked for the option to lock the subject lines of their threads but yea no avail lol
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LizardWizard
GnomeGrower




Registered: 01/07/15
Posts: 13,692
Loc: the parking lot
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You shouldn't spray with iso mix, that's a fire hazard. Spray with soapy or even just plain water. Just make sure you rinse out the bottle and spray nozzle with a 1/10th strength bleach solution every week.
CA Kratom Weed A little fun and games ppl
-------------------- The best things in life can be smelled on one's fingers.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Quote:
LizardWizard said: You shouldn't spray with iso mix, that's a fire hazard. Spray with soapy or even just plain water. Just make sure you rinse out the bottle and spray nozzle with a 1/10th strength bleach solution every week.
CA Kratom Weed A little fun and games ppl
Yeah, I switched from misting with my ISO mix to soapy water. Didn’t really think about safety at first. Unfortunately I have to take my SAB down between uses so I clean it out with my ISO mix before setting it up and mist with a mild dawn soap/water mix when working.
Just enjoyed some White Maeng Da tea myself
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LizardWizard
GnomeGrower




Registered: 01/07/15
Posts: 13,692
Loc: the parking lot
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Can't say I enjoy kratom teas. I capsulate (only the more sedating varieties) so I don't have to taste it. White maeng da would likely give me anxiety. Just like cerebral highs 
Getting older sucks.
-------------------- The best things in life can be smelled on one's fingers.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Quote:
LizardWizard said: Can't say I enjoy kratom teas. I capsulate (only the more sedating varieties) so I don't have to taste it. White maeng da would likely give me anxiety. Just like cerebral highs 
Getting older sucks.
Yes it does. I usually drink my WMD tea in the morning. I’ll steep it with some Irish breakfast tea and honey. Then turn it into an iced tea. Add a little lemon and you can barely taste it. Always have a variety of Kratom around though:-)
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Took a couple pinners yesterday to attempt cloning and with a freeze coming through I took the rest today.
Pinners from yesterday are showing growth:
Unfortunately I only had two more plates made up so I put multiple pieces of stem on each plate. Have the agar made, just waiting for more dishes to arrive tomorrow. Hopefully I’ll be able to isolate at least one clean culture.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Trying to learn what to look for in my cultures. Here are two transfers taken from the same plate at the same time. Transfer 1 appears to be more uniform and consistent in growth. Lots of rhizomorphic growth on the outer edges and has stayed flush with the agar. Transfer 2 looks good as well but still needs at least one more transfer. Growth is not as consistent and raised areas indicate bacterial growth. Any feedback is greatly appreciated!
Transfer 1: (IMO the better of the two.)



Transfer 2:



Thanks in advance,
BB
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gizmodo
Woodland Creature




Registered: 06/21/18
Posts: 2,064
Loc: Behind You
Last seen: 2 years, 8 months
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Well it depends on what you are looking for are you looking for contamination or indicators of healthy growth? Vigor, aggressive nature and strong rope like branches indicate a strong or lively strain culture. If you are looking for contamination you're looking for mycelium leaping over portions, growing uniformly, odd colors that aren't inherent to the actual strain, cessation of outward colonization, stuff like that. From what I see at least I feel like transfer two looks better than transfer one because of its dense networks, seemingly uniform growth, and rope like nature.
-------------------- One must be mindful to maintain their balance on the slippery earth. Bod's Comprehensive Agar Resource Gizmodo's Market Stall Say No To Grow Kits
Edited by gizmodo (10/21/18 07:32 PM)
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: This is WCA. [Re: gizmodo] 1
#25556631 - 10/21/18 10:10 PM (5 years, 3 months ago) |
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Quote:
gizmodo said: Well it depends on what you are looking for are you looking for contamination or indicators of healthy growth? Vigor, aggressive nature and strong rope like branches indicate a strong or lively strain culture. If you are looking for contamination you're looking for mycelium leaping over portions, growing uniformly, odd colors that aren't inherent to the actual strain, cessation of outward colonization, stuff like that. From what I see at least I feel like transfer two looks better than transfer one because of its dense networks, seemingly uniform growth, and rope like nature.
Thanks Giz,
Definitely transferring from both. The reason I liked #1 better is because it seems in the middle. Not too ropey, not too fluffy and there’s a lot going on at the edges. Definitely like #2 and agree that it looks strong but someone mentioned there is bacteria under fluff and it seems to have areas that have fluffed up.
Again I’ll be taking transfers from each, was just curious about my judgement at this point.
Thanks again!
BB
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Cultivation General Discussion [Re: Inocuole]
#25557230 - 10/22/18 07:56 AM (5 years, 3 months ago) |
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Quote:
gizmodo said:
Vigor, aggressive nature and strong rope like branches indicate a strong or lively strain culture.
No offense but can you elaborate on that a lot more please, because I have data and images that says otherwise, in addition not all mycelium aggregate that much. I've seen various mycelium turn from 'healthy' tomentose to aggressive rhizomorphs due to bacteria, toxins, and also low nutrition.
Healthy tomentose growth will populate the entire surface area, extreme rhizomorphs do not.

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@Brain Bulb
Your growth isn't what I would call rhizomorphic as such, more linear growth. I can also see improvement due to accumilation. The growth appears to start as 'low nutrition', however the further it grows out the more tomentose it becomes.
Here is some linear surface growth, which I would not call rhizomorphic (which is more rooty).

Here is an example of low nutrients > high nutrients (enzymes).

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gizmodo
Woodland Creature




Registered: 06/21/18
Posts: 2,064
Loc: Behind You
Last seen: 2 years, 8 months
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Re: Cultivation General Discussion [Re: Ferather]
#25557295 - 10/22/18 08:34 AM (5 years, 3 months ago) |
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That's what I read in Gourmet and medicinal mushrooms. 
Other factors come into play but I would wager that rope like mycelium can serve as an indicator of culture health. That doesn't mean there are no contaminates in the petri dish though and some varieties do not favor rope like growth. From what I remember a healthy strain starts out rhizomorphic and goes linear after awhile of transferring or if issues occur or something similar. Are these plates an example of linear growth? Which would be an indication of a weaker and less healthy mycelium or? Maybe you can help clear that up. Maybe my identification of a rope like bundle of hyphal connections is wrong? I know I don't know how to identify anything like that in rhizomorphic growth which is favored over linear growth for indication of proper health. I'll reread the chapter later today and see if I misread rope like hyphal bundles being an indicator of strength or something similar.
Picture
Edited by gizmodo (10/22/18 09:01 AM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: gizmodo]
#25557352 - 10/22/18 09:09 AM (5 years, 3 months ago) |
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It happens for various reasons and purposes. The main difference is the splitting, where dense rhizomorphs are clumped into a rope. In the case of a low nutrition rhizomorph, it will push out and then it should split when more nutrients are available.
You can also see rhizomorphs in dirt, where the mycelium is looking for pockets of food in inert materials. It can also aggregate if there is everything but one key nutrient, lets say it's missing nitrogen.
I've also seen the same mechanism triggered in response to unfavorable bacteria.
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Slightly off topic, but this image might help.
Edited by Ferather (10/22/18 09:25 AM)
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Cultivation General Discussion [Re: Ferather] 2
#25557640 - 10/22/18 11:11 AM (5 years, 3 months ago) |
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I’m not a mycologist, but from what I’ve seen with my cultures, the specimens that are more rhizomorphic/linear tend to colonize faster.
I also agree that not all mycelium grows the same. Here is a Panaeolus culture that threw me off. Initially I thought it was mold at but it’s also the first Pan culture I’ve done.

Some key things I’ve learned so far:
1.) Sterilization is key. Don’t try to take short cuts, they’ll bite you in the long run. 2.) Expect the unexpected and be prepared to deal with it 3.) The info provided from the Trusted Cultivators works if used correctly. They wouldn’t be TC’s if they didn’t know what they were doing. 4.) Not all methods will work for everyone. Find one that works for you. 5.) Don’t over think things. Yes this is science but have fun.
My two cents...
BB
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25557728 - 10/22/18 12:04 PM (5 years, 3 months ago) |
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I actually agree entirely with your first sentence, under certain circumstances (such as agar, and it's purpose). The richer the agar is, the thicker and slower the mycelium, there will be no-little rhizomorphs.
This is due to splitting and branching to nutrients (concentration, amount per gram).
---- ----
Fruiting medias:
If a substrate nutrient deficiency is limiting enzyme production and general growth, enrichment can speed up growth and-or population. In some cases the physical outgrowth may not be faster, maybe slower, but the physical population rate higher.
The same can be said for a substrates pH, which can limit enzyme stability and total activity.
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Low nutrition (A: mostly all carbon, missing nutrients | B: low pH, enriched):

Nutritional (A: paper pellets, enriched | B: pine, optimal pH, enriched):

Both outgrowth and population rates increased. Total biological efficiency increased.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Cultivation General Discussion [Re: Ferather]
#25557887 - 10/22/18 01:21 PM (5 years, 3 months ago) |
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I was just referring to agar cultures. Substrate and fruiting media’s are going to vary on the species.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25557894 - 10/22/18 01:23 PM (5 years, 3 months ago) |
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I know, I just didn't want you to standardize that rule to everything.
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superbarnie
Stranger

Registered: 03/27/16
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Re: Cultivation General Discussion [Re: Ferather]
#25559378 - 10/22/18 11:09 PM (5 years, 3 months ago) |
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Hi, im looking to make the jump from pasty plates to petri plates. The question is should I use plastic ones or glass? Plastic is cheaper but glass is reusable.
WHat are your thoughts?
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