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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Tookitooki]
#25542318 - 10/16/18 02:57 PM (5 years, 3 months ago) |
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Quote:
Tookitooki said: Is it possible to use a refractometer, to gauge the concentration of grain water?
Kind of. A refractometer for brewing is calibrated for glucose. When measuring wort there's a correction that has to be applied because of all the maltose. So kind of but not really. A hydrometer would be better tool
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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Re: Cultivation General Discussion [Re: bodhisatta] 1
#25542329 - 10/16/18 02:59 PM (5 years, 3 months ago) |
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I usually just dunk witches in mine. If they float, there's enough nutrition for the myc to thrive.
-------------------- Stop cold shocking your mycelium! Hot Spawn - Get It On 
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: bodhisatta] 1
#25542337 - 10/16/18 03:01 PM (5 years, 3 months ago) |
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Normal stuff for cubes on agar.

I wouldn't specifically aim for tomentose or use recipes that encourage that kind of growth. Also you really need not even measure pH for simple hobby growing of cubes.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 10 hours
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Re: Cultivation General Discussion [Re: Ferather]
#25542371 - 10/16/18 03:17 PM (5 years, 3 months ago) |
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Quote:
Tookitooki said: Is it possible to use a refractometer, to gauge the concentration of grain water?
Quote:
Ferather said: You can see the reflective edge of what I call slime in image 1, (12 o'clock). The same area in image 2 has a wavy lighter coloured line running. There is also a light blob at 6 o'clock in image 2. It could be condensate as you mentioned, does it run off if you tilt it?
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Some images and recipes for reference. You may need to adjust the recipe's to suit.
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Quote:
Mycolorado said:
Just wanted to bring this to the attention of mush cult. katbusa and Ferather have been experimenting with tea agar to clean up contaminated spawn and cultures. I thought it would fit in over here as a great alternative to antibiotic agar...tea is readily available and not everyone wants to use antibiotics. Also, bacterial contams are often present when starting from prints and syringes, and can really be discouraging to those starting out. I modified the original recipe to use less tea as the original amount seemed a bit excessive.
Here are a couple APE cultures and a malabar. All had some degree of bacterial contam and are now growing clear. Growth is very slow on this medium but will result in a clean culture.
NOTE: Spores will not germinate well on this agar!

200ml H2O 4.25g black tea (2 bags) 2g ME 4g Agar
Original post here
My recipe (for wood lovers) here: T-Gel antibacterial agar.
Notes:
For Cubensis, and other types use Mycolorado's recipe. Growth should be tomentose, not rhizomorphic. You may need to adjust the end pH.
tomentose myc hides contams way too well. Stop telling noobs to look for tomentose
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Tookitooki
Mycological Fabricator



Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Cultivation General Discussion [Re: bodhisatta]
#25542436 - 10/16/18 03:42 PM (5 years, 3 months ago) |
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Quote:
bodhisatta said:
Quote:
Tookitooki said: Is it possible to use a refractometer, to gauge the concentration of grain water?
Kind of. A refractometer for brewing is calibrated for glucose. When measuring wort there's a correction that has to be applied because of all the maltose. So kind of but not really. A hydrometer would be better tool
Thanks I'll look in to it more. I just want to compare my own batches. Just like the theory to lower nutes for faster growth. I think I'll experiencing to many nutes. I'll have pictures up soon.
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SunnyDayze
Finder of shrubberies



Registered: 12/30/17
Posts: 2,230
Loc: Canada
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Re: Cultivation General Discussion [Re: grainbrain]
#25542437 - 10/16/18 03:43 PM (5 years, 3 months ago) |
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Quote:
grainbrain said: I usually just dunk witches in mine. If they float, there's enough nutrition for the myc to thrive.
And if you have no witches, what else could float in water?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Tookitooki]
#25542442 - 10/16/18 03:45 PM (5 years, 3 months ago) |
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Quote:
Tookitooki said:
Quote:
bodhisatta said:
Quote:
Tookitooki said: Is it possible to use a refractometer, to gauge the concentration of grain water?
Kind of. A refractometer for brewing is calibrated for glucose. When measuring wort there's a correction that has to be applied because of all the maltose. So kind of but not really. A hydrometer would be better tool
Thanks I'll look in to it more. I just want to compare my own batches. Just like the theory to lower nutes for faster growth. I think I'll experiencing to many nutes. I'll have pictures up soon.
A total dissolved solids meter and a hydrometer would be where I would start.
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: bodhisatta]
#25542470 - 10/16/18 03:53 PM (5 years, 3 months ago) |
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All my grain substrates are being colonized in jars with mouths that aren't wide mouths (meaning I cant make it slip out as a brick). Is shaking what needs to be done so it colonizes on the inside? Or is it just to speed up the process?
I won't be able to get the cake out without smashing the jar. Wide mouth jars cost like 5 times as much and were half the size so no way I'm using that.
-------------------- One day I will evolve from agar mushroom grower
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 10 hours
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Grain jars dont need to come out as a cake they are mixed with bulk sub. The shake is just to increase speed and check for contams
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
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Fruiting regular mouth quart jars sucks dick for the cleanup.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: bodhisatta]
#25542560 - 10/16/18 04:27 PM (5 years, 3 months ago) |
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Quote:
bodhisatta said: Normal stuff for cubes on agar.

I wouldn't specifically aim for tomentose or use recipes that encourage that kind of growth. Also you really need not even measure pH for simple hobby growing of cubes.
Those are all aerial puff balls, the images I have provided are all flat(ish), as they should be. In addition, not all mycelium produce the rhizomorphs being described.
Here is an image, where you can compare the difference.
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25542573 - 10/16/18 04:30 PM (5 years, 3 months ago) |
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Quote:
tryptkaloids said: Grain jars dont need to come out as a cake they are mixed with bulk sub. The shake is just to increase speed and check for contams
The kit I started doing shrooms with had no bulk sub so I don't really know much about that yet. What are the chances of contams like when I put the colonized grain on top of coir+verm and then coir+verm on top of that.
Does bulk substrate increase the yield or something? I know it isn't absolute necessity but there is probably a very good reason why all of you do it.
-------------------- One day I will evolve from agar mushroom grower
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TheUltimateBohab
Fat and Slobby


Registered: 09/05/18
Posts: 319
Loc: LV-426
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is it possible to grow morels inside? or really at all? i find them in the wild on occasion...
-------------------- *DISCLAIMER* Everything I say on here is BULLSHIT!! I lie so I can kick it...
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 10 hours
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Quote:
IntergalacticSpore said:
Quote:
tryptkaloids said: Grain jars dont need to come out as a cake they are mixed with bulk sub. The shake is just to increase speed and check for contams
The kit I started doing shrooms with had no bulk sub so I don't really know much about that yet. What are the chances of contams like when I put the colonized grain on top of coir+verm and then coir+verm on top of that.
Does bulk substrate increase the yield or something? I know it isn't absolute necessity but there is probably a very good reason why all of you do it.
mushrooms are 90% water. The bulk sub supplies that water. How many times do we need to tell you kits suck before you understand?
Quote:
TheUltimateBohab said: is it possible to grow morels inside? or really at all? i find them in the wild on occasion...
Not yet... We try... Https://www.shroomery.org/forums/showflat.php/Number/9479734
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cultivation General Discussion [Re: Mateja]
#25543133 - 10/16/18 08:21 PM (5 years, 3 months ago) |
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Hey does anyone know where I might find some 4oz polypropylene containers with screw on lids? Kind of like the same idea as ziplock twist-n-lock but the size of glad mini rounds?
I've been scouring Amazon for months now cause I'm getting real tired of putting foil and paper towels on everytime I cook up a batch of no pour plates
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: ComebackKid]
#25543153 - 10/16/18 08:28 PM (5 years, 3 months ago) |
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have you tried 1/2 pint wide mouth jars? they are a little bigger than pasty plates.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Ferather]
#25543160 - 10/16/18 08:31 PM (5 years, 3 months ago) |
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Quote:
Ferather said:
Quote:
bodhisatta said: Normal stuff for cubes on agar.

I wouldn't specifically aim for tomentose or use recipes that encourage that kind of growth. Also you really need not even measure pH for simple hobby growing of cubes.
Those are all aerial puff balls, the images I have provided are all flat(ish), as they should be. In addition, not all mycelium produce the rhizomorphs being described.
Here is an image, where you can compare the difference.
Cool,.. well morphology on agar is basically meaningless. And not to measure dicks but we can compare what we've fruited from our cultures. Im happy with my results

I can make pretty plates too, and i don't have to link to ones i found on the internet.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cultivation General Discussion [Re: JHOVA]
#25543175 - 10/16/18 08:38 PM (5 years, 3 months ago) |
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I have 1/4 pints that could work but there's just something about using plastic that you just cant beat
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: ComebackKid]
#25543186 - 10/16/18 08:41 PM (5 years, 3 months ago) |
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there was some discussion in josex thread about containers off ebay/amazon. i think there was one you could get in the US.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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RomeoPapa
Jackass of All Trades



Registered: 05/14/17
Posts: 1,583
Loc: In the middle
Last seen: 1 year, 2 months
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Quote:
TheUltimateBohab said: is it possible to grow morels inside? or really at all? i find them in the wild on occasion...
Find out how to reliably fruit morels indoors and you will be a millionaire
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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