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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Jeezus Christmas you really need to lighted up man. If you ever read my 3 feet long posts you would know that I dont take my self that Sirius and that im half joking abiut caling neself a SCIEN5IST but he'll yeah I'm a scientist when I feel like it.
Like I told you times before lighten up a little and I don't think you should stop responding to I don't mind constructive criticism. You simply don't know what I mean with the double lid and it will become clear to you when I snap photos. Do go and put me on ignore now before I show you
-------------------- Cakes inside Water Tub
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
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Re: Cultivation General Discussion [Re: Mateja]
#25540590 - 10/15/18 09:35 PM (5 years, 3 months ago) |
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-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25540602 - 10/15/18 09:39 PM (5 years, 3 months ago) |
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It could be benefiting to other growers who have the same BRF jars as me. I'm just saying that the way I prepare my jars is pretty much the ONLY way it can be done. I follow PF Tek to the T and modify only what is necessary to even be able to make BRF jars
Pics coming soon it's not off topic if it's a little bit off topic :PPppPppP
It's just irritating that some people always have to comment thing like: "you are like this you are like that" implying implying implying... I am..
-------------------- Cakes inside Water Tub
Edited by Mateja (10/15/18 09:41 PM)
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
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Re: Cultivation General Discussion [Re: Mateja]
#25540617 - 10/15/18 09:45 PM (5 years, 3 months ago) |
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I still dont get why the extra lid is neccesary...
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25540631 - 10/15/18 09:49 PM (5 years, 3 months ago) |
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I'm on the subway wait 20 min hahaha the jars require both lids or it doesn't work
-------------------- Cakes inside Water Tub
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25540652 - 10/15/18 09:58 PM (5 years, 3 months ago) |
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Quote:
tryptkaloids said: I still dont get why the extra lid is neccesary...
It's not, evidenced by everyone else doing PF Tek successfully for decades plus without it. I went 23/24 on my first go and I'm not even a scientist. Maybe he's using soup cans or something instead of 1/2 pints. Whatever, though.
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Mateja]
#25540689 - 10/15/18 10:14 PM (5 years, 3 months ago) |
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These are my BRF jars (or fake scientist containers) however you wanna call em

See? The needle would barely scratch the top of the substrate if I was Slightly Stoopid (great band btw) to try to poke trough the outer lid. (And a have no clue why I would want to destroy the lids of these nice jars by poking holes in them)
This is how the 3 parts of the jar look

And this is how the jar looks with just the inner piece on. It has nothing to keep it secure to the jar rim so naturally the screw top goes on top...
-------------------- Cakes inside Water Tub
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: Mateja] 2
#25540751 - 10/15/18 10:44 PM (5 years, 3 months ago) |
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Right. So we use a single lid/ring combo. Do whatever works for you, but that's not, "pretty much how PF Tek instructs" and is unnecessary unless jar rings are banned in your country or something.
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mushroomnate
Pstranger



Registered: 05/17/17
Posts: 3,100
Last seen: 2 days, 13 hours
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Re: Cultivation General Discussion [Re: Mateja]
#25540760 - 10/15/18 10:48 PM (5 years, 3 months ago) |
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That's the first time I've seen a lid like that. But hey,if it works... 
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Like I said in the previous post, only difference between PF and this is I don't have a ring that holds the 'inoculation hatch' secured to the jar I have the 'full' lid. And this is the only practical way to do it with these 1/2 pint jars. And a haven't seen the rings in my country only the sen it from N.America and from Tek's on Internet.
-------------------- Cakes inside Water Tub
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
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Re: Cultivation General Discussion [Re: stareatclouds] 1
#25541368 - 10/16/18 08:29 AM (5 years, 3 months ago) |
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Quote:
stareatclouds said:
Quote:
tryptkaloids said: I still dont get why the extra lid is neccesary...
It's not, evidenced by everyone else doing PF Tek successfully for decades plus without it. I went 23/24 on my first go and I'm not even a scientist. Maybe he's using soup cans or something instead of 1/2 pints. Whatever, though.
see, i knew it was due to his situation, you could have just said all you have is single piece lids
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25541922 - 10/16/18 12:36 PM (5 years, 3 months ago) |
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This is one of the A2A transfers I did recently. Still learning about mycelium but this looks good to me. Would you agree or no?
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25541933 - 10/16/18 12:38 PM (5 years, 3 months ago) |
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I know I need to sterilize the wood chips I’m planning to use as casing for the Ps. Cyanescens I’m trying to grow. Can I sterilize them like coir or do they have to be PC’d?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25541996 - 10/16/18 01:01 PM (5 years, 3 months ago) |
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Quote:
Brain Bulb said: This is one of the A2A transfers I did recently. Still learning about mycelium but this looks good to me. Would you agree or no?

I see slime at 12 o'clock, and the effected area has differential growth, it actually looks like it's gone under it. I also see a small bit at 6 o'clock. The rhizomorphs may be normal for that agar recipe, however I don't like to see rhizomorphs at all. Likely still infected I'm afraid.
Quote:
Brain Bulb said: ..Can I sterilize pasteurize them like coir or do they have to be PC’d (sterilized)?
You can pasteurize them like coir, unless enriched with a starch-sugar based additive (such as grain flour, or bran), then PC.
Edit: If outdoors, see Bod's advice below.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25541999 - 10/16/18 01:03 PM (5 years, 3 months ago) |
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Quote:
Brain Bulb said: I know I need to sterilize the wood chips I’m planning to use as casing for the Ps. Cyanescens I’m trying to grow. Can I sterilize them like coir or do they have to be PC’d?
Outdoors? Just soak em. Thats it.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Cultivation General Discussion [Re: bodhisatta]
#25542059 - 10/16/18 01:23 PM (5 years, 3 months ago) |
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Quote:
bodhisatta said:
Quote:
Brain Bulb said: I know I need to sterilize the wood chips I’m planning to use as casing for the Ps. Cyanescens I’m trying to grow. Can I sterilize them like coir or do they have to be PC’d?
Outdoors? Just soak em. Thats it.
These will be for an indoor grow but I’ve made a outdoor bed I’m going to use a LI on.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Cultivation General Discussion [Re: Ferather]
#25542074 - 10/16/18 01:30 PM (5 years, 3 months ago) |
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Quote:
Ferather said:
Quote:
Brain Bulb said: This is one of the A2A transfers I did recently. Still learning about mycelium but this looks good to me. Would you agree or no?

I see slime at 12 o'clock, and the effected area has differential growth, it actually looks like it's gone under it. I also see a small bit at 6 o'clock. The rhizomorphs may be normal for that agar recipe, however I don't like to see rhizomorphs at all. Likely still infected I'm afraid.
Quote:
Brain Bulb said: ..Can I sterilize pasteurize them like coir or do they have to be PC’d (sterilized)?
You can pasteurize them like coir, unless enriched with a starch-sugar based additive (such as grain flour, or bran), then PC.
Edit: If outdoors, see Bod's advice below.
I’ll have to see if they are enriched. They’re alder wood chips for smoking.
Agar is Bod’s grain soak recipe. I’ll double check when I get home but I believe what you’re referring to as slime is condensation. Poured my agar a little too hot and didn’t let it sit long enough. Was looking at taking transfers from the 3 & 9 o’clock positions.
I thought rhizomorphs were what you wanted. The rhizo is the ropey myc correct?
Thanks for the correction on my verbiage as well. Still learning!
BB
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25542165 - 10/16/18 02:01 PM (5 years, 3 months ago) |
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Ferather doesnt grow mushrooms, just myc rhizomorphs are a good thing in the right context. The 'slime' he's referring to is just weak spots in the culture. I'd be happy to transfer from that
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Tookitooki
Mycological Fabricator



Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25542168 - 10/16/18 02:02 PM (5 years, 3 months ago) |
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Is it possible to use a refractometer, to gauge the concentration of grain water?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Tookitooki]
#25542235 - 10/16/18 02:25 PM (5 years, 3 months ago) |
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You can see the reflective edge of what I call slime in image 1, (12 o'clock). The same area in image 2 has a wavy lighter coloured line running. There is also a light blob at 6 o'clock in image 2. It could be condensate as you mentioned, does it run off if you tilt it?
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Some images and recipes for reference. You may need to adjust the recipe's to suit.
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Quote:
Mycolorado said:
Just wanted to bring this to the attention of mush cult. katbusa and Ferather have been experimenting with tea agar to clean up contaminated spawn and cultures. I thought it would fit in over here as a great alternative to antibiotic agar...tea is readily available and not everyone wants to use antibiotics. Also, bacterial contams are often present when starting from prints and syringes, and can really be discouraging to those starting out. I modified the original recipe to use less tea as the original amount seemed a bit excessive.
Here are a couple APE cultures and a malabar. All had some degree of bacterial contam and are now growing clear. Growth is very slow on this medium but will result in a clean culture.
NOTE: Spores will not germinate well on this agar!

200ml H2O 4.25g black tea (2 bags) 2g ME 4g Agar
Original post here
My recipe (for wood lovers) here: T-Gel antibacterial agar.
Notes:
For Cubensis, and other types use Mycolorado's recipe. Growth should be tomentose, not rhizomorphic. You may need to adjust the end pH.
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