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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23853136 - 11/21/16 01:25 AM (7 years, 2 months ago)

Quote:

noob47 said:
If you cloned an isolate would you still have an isolate of that tissue sample?



Why would you clone an isolate:crazy:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: cronicr]
    #23853138 - 11/21/16 01:27 AM (7 years, 2 months ago)

Haha im not got insomnia and was thinking about shit haha


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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23853141 - 11/21/16 01:29 AM (7 years, 2 months ago)

:lolsy::crondance:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineTiamo
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Re: Cultivation General Discussion [Re: cronicr]
    #23854146 - 11/21/16 12:11 PM (7 years, 2 months ago)

Can I use agar to inoculate PF cakes? Do I skip the dry verm layer and just drop a wedge in there?


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If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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Offlinewtfcrazymofo
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Re: Cultivation General Discussion [Re: Tiamo]
    #23854154 - 11/21/16 12:17 PM (7 years, 2 months ago)

Is it cool to do a pc run with some pour agar for 2.25 hours because I want to run whole oats at the same time?


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineKenetic
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Re: Cultivation General Discussion [Re: wtfcrazymofo]
    #23854166 - 11/21/16 12:24 PM (7 years, 2 months ago)

tiamo, I'd say yes, just use a filtered lid instead of a verm barrier, and to wtfcrazymofo I'd say no.  You will probably caramelized the nutrients, rendering them useless. I've never actually pc'd agar that long, I'm simply giving you an educated guess


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


Edited by Kenetic (11/21/16 02:47 PM)


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Offlinetombosley8
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Re: Cultivation General Discussion [Re: wtfcrazymofo]
    #23854168 - 11/21/16 12:25 PM (7 years, 2 months ago)

pretty sure you will destroy the sugar or nutes with much more than 45 min for no pour.

I think pour agar can go slightly longer since there is so much material together it doesn't overheat as fast.

But I wouldn't push it.


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Edited by tombosley8 (11/21/16 12:36 PM)


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OfflineA.RichardTrickle
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Registered: 11/04/16
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Re: Cultivation General Discussion [Re: wtfcrazymofo]
    #23854172 - 11/21/16 12:26 PM (7 years, 2 months ago)

It is OFFICIAL friends, the cake grows will be done forever in a couple of weeks!!!  :grin:
My pink buffalo and lizzard king germed on my pasty plates, hooray.
Started 20 jars of red milo and more agar plates today, bye bye cakes!
I am so fucking stoked you guys, I have been waiting YEARS for this.
Dick


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"When eating shit, it is best not to nibble.  Bite, Chew. Swallow. Repeat."
"If you're making love to your old lady, someone else is fucking her"
"Douchebags are children who never grew up, like Sheeklette, we should pity them."
[quote]Niffla said:
[quote]A.RichardTrickle said:
Dick[/quote]
http://www.youtube.com/v/kbwNUOUy-3c[/quote]


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Offlinemrmazdarx9
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Re: Cultivation General Discussion [Re: Kenetic]
    #23854181 - 11/21/16 12:31 PM (7 years, 2 months ago)

Quote:

kenetic said:
Pismo, I'd say yes, jupcse a filtered lid instead, and to wtf I'd say no.  You will probably caramelized the nutrients, rendering them useless. I've never actually pc'd agar that long, I'm simply giving you an educated guess



I dont know if im drunk or you but I didnt get that if were both drunk then we are both winning


--------------------
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Right Here
If you use "SWIM" you should DROWN


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OfflineKenetic
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Re: Cultivation General Discussion [Re: mrmazdarx9]
    #23854500 - 11/21/16 02:45 PM (7 years, 2 months ago)

I was responding to two people and I had a few typos....

fixed


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Offlinetump
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Re: Cultivation General Discussion [Re: Kenetic]
    #23855044 - 11/21/16 05:33 PM (7 years, 2 months ago)

You can cook non sugar based agar for that long. I've done so before. Malt, or corn starch agar for the win.


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InvisibleMunchauzen
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Re: Cultivation General Discussion [Re: tump] * 1
    #23855061 - 11/21/16 05:40 PM (7 years, 2 months ago)

Quote:

tump said:
You can cook non sugar based agar for that long. I've done so before. Malt, or corn starch agar for the win.



fyi, malt is a sugar.

Quote:

Malting grains develops the enzymes required for modifying the grain's starches into sugars, including the monosaccharide glucose, the disaccharide maltose, the trisaccharide maltotriose, and higher sugars called maltodextrines.




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OfflineGreg
always learning
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Re: Cultivation General Discussion [Re: Munchauzen] * 1
    #23855096 - 11/21/16 05:54 PM (7 years, 2 months ago)

:whathesaid:
Depending on the pH of the media starches can also hydrolyze to simple sugars and subsequently caramelize in a PC.
Basically just avoid PCing agar or LC for excessive amounts of time if possible.


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InvisibleMunchauzen
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Re: Cultivation General Discussion [Re: Greg] * 1
    #23855110 - 11/21/16 05:57 PM (7 years, 2 months ago)

Quote:

Greg said:
:whathesaid:
Depending on the pH of the media starches can also hydrolyze to simple sugars and subsequently caramelize in a PC.
Basically just avoid PCing agar or LC for excessive amounts of time if possible.



I had a feeling it would because of the amylopectin content, which is a type of glucose. I just wasn't sure, so I didn't want to speak without assurance.


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OfflineGreg
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Re: Cultivation General Discussion [Re: Munchauzen]
    #23855155 - 11/21/16 06:13 PM (7 years, 2 months ago)

Makes me want to go break out the Benedict's reagent and find the point cornstarch starts to actually break down in a PC :lol:


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OfflineDactylium
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Registered: 08/12/16
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Re: Cultivation General Discussion [Re: Greg]
    #23855156 - 11/21/16 06:14 PM (7 years, 2 months ago)

I thought you guys might get a kick out of this:


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InvisibleSubnet Mask
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Registered: 05/08/15
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Re: Cultivation General Discussion [Re: Dactylium]
    #23855162 - 11/21/16 06:15 PM (7 years, 2 months ago)



What a tool bag.


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Offlinetombosley8
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Re: Cultivation General Discussion [Re: tombosley8]
    #23855167 - 11/21/16 06:16 PM (7 years, 2 months ago)

Anyone??
Quote:

tombosley8 said:
any input?
Quote:

tombosley8 said:
When storing plates in the fridge, should I use one of those soft lunch boxes to insulate them and prevent temp fluctuations?

What kind of lunch box or other containers do you guys use?

If they are stacked up a few high will they be ok without insulation?

How long will a slightly thick poured plate last in the fridge before drying out?




On another note, If I pick 1 or two mushrooms at the perfect time and I don't want to use a dehydrator for just 2 fruits would storing them in the fridge preserve potency the best until a larger batch comes in to dry?




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OfflineDactylium
Don't touch me I'm sterile


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Re: Cultivation General Discussion [Re: Dactylium]
    #23855191 - 11/21/16 06:27 PM (7 years, 2 months ago)

Whoops just realized someone already made a thread about this


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Offlinewtfcrazymofo
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Registered: 07/26/15
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Re: Cultivation General Discussion [Re: tombosley8]
    #23855195 - 11/21/16 06:29 PM (7 years, 2 months ago)

Fresh ones rule. dried ones are not the same(less).  I would give them 2 weeks in a brown bag fridge.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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