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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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O0ZE
Ascended Drone for the hive



Registered: 02/28/18
Posts: 321
Loc: Getting Toasted in the sun
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: bodhisatta]
#25432745 - 09/03/18 11:04 AM (5 years, 4 months ago) |
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what is the unit that's being gauged do... ah, so that's a no shit sterilizer. 20 PSI better? so your stuff is cleaner using that, or are we talking professional choice? if it's a professional choice what are all the whys?
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Not everything that counts is countable and not everything that is countable counts.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: O0ZE]
#25432748 - 09/03/18 11:06 AM (5 years, 4 months ago) |
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The AA 25x. Got it used for 200 dollars so i was wary of how well it would work right out of the box
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: bodhisatta]
#25432906 - 09/03/18 12:28 PM (5 years, 4 months ago) |
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Quote:
gizmodo said: They do look rather wet and creamy. Was that your indicator tryp?
Wet spots, uncolonized grains and discoloration.
This is condensation. this looks more like bacterial goop
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: tryptkaloids] 1
#25432914 - 09/03/18 12:32 PM (5 years, 4 months ago) |
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  This is bacterial goop. My go-to indicator
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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gizmodo
Woodland Creature




Registered: 06/21/18
Posts: 2,064
Loc: Behind You
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25432926 - 09/03/18 12:37 PM (5 years, 4 months ago) |
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The ole goop spoop.
-------------------- One must be mindful to maintain their balance on the slippery earth. Bod's Comprehensive Agar Resource Gizmodo's Market Stall Say No To Grow Kits
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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Re: Cultivation General Discussion [Re: gizmodo]
#25432961 - 09/03/18 12:55 PM (5 years, 4 months ago) |
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It's gonna be weird goin back to cubes, I've gotten so use to funky lookin truffle jars that I might end up lettin somethin bacterial slide past me on accident lol
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
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Re: Cultivation General Discussion [Re: bodhisatta]
#25434400 - 09/04/18 06:09 AM (5 years, 4 months ago) |
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Quote:
bodhisatta said: The AA 25x. Got it used for 200 dollars so i was wary of how well it would work right out of the box
One thing I found with my AA's is to tighten the lid down just a little before venting...Get the lid placed evenly and tight enough to stay on well but after venting when everything is nice and hot then tighten the lid down snug. New AA's will not seal perfectly for a few rounds.
I picked up an AA 910 for $75 Canadian recently and that thing is awesome for agar and blender jars...Its a mini sterilizer.
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Shadowboxing the apocalypse and wandering the land
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RomeoPapa
Jackass of All Trades



Registered: 05/14/17
Posts: 1,583
Loc: In the middle
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Re: Cultivation General Discussion [Re: Crispykoot]
#25434596 - 09/04/18 09:00 AM (5 years, 4 months ago) |
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Nice Crispy. You got a steal on a "baby tank". I can't Express enough how much I love my AA921.
I agree that the first couple times it didn't seal properly and I was about to have a literal melt down. Considering what I paid for the damn thing.
The plug in model like Bod has would be super fuvking sweet though!
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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Timmy Meow



Registered: 04/05/15
Posts: 546
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Re: Cultivation General Discussion [Re: RomeoPapa]
#25435090 - 09/04/18 01:16 PM (5 years, 4 months ago) |
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Is this what monokaryotic mycelium looks like? (The middle plate)

If not, does anyone have pics of how monokaryotic mycelium looks?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Timmy Meow]
#25435250 - 09/04/18 03:04 PM (5 years, 4 months ago) |
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No, and no. You won't accidentally get monokaryotic mycelium. It's damn near impossible in my efforts trying to deliberately grow it by diluting spores. To get monokaryotic mycelium I would imagine you need a dissection (micromanipulator) microscope. I've never once seen a picture of monokaryotic cubensis mycelium.
You have dikaryotic mycelium. Sometimes it looks light and watery the first few transfers. Especially the leading edge of the growth.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: bodhisatta]
#25435261 - 09/04/18 03:11 PM (5 years, 4 months ago) |
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I've seen this happen when nutes are too low
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Timmy Meow



Registered: 04/05/15
Posts: 546
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25435444 - 09/04/18 04:37 PM (5 years, 4 months ago) |
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Yeah I think bod is on point since it was the leading edge of streaked multispores. First transfer from MS.
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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Re: Cultivation General Discussion [Re: bodhisatta]
#25435598 - 09/04/18 05:38 PM (5 years, 4 months ago) |
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Quote:
bodhisatta said: No, and no. You won't accidentally get monokaryotic mycelium. It's damn near impossible in my efforts trying to deliberately grow it by diluting spores. To get monokaryotic mycelium I would imagine you need a dissection (micromanipulator) microscope. I've never once seen a picture of monokaryotic cubensis mycelium.
You have dikaryotic mycelium. Sometimes it looks light and watery the first few transfers. Especially the leading edge of the growth.
Didn't RR or Workman post pics of monokaryotic mycelium at one point? I've read about it a few times for sure, just not sure who posted. It's been like 2 years since I've seen anything mentioned about monokaryotic myc and I'm lucky if I remember what I ate for breakfast some days, so yeah lol
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: 00Burnout] 1
#25435650 - 09/04/18 05:52 PM (5 years, 4 months ago) |
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I haven't seen a pic. if you count a micrograph then this post counts
Quote:
RogerRabbit said: I had an eyepiece camera for my cheapie scope, but was never satisfied with the pictures it took. This one is a trinocular compound microscope with a CCD tube, holding a 3 megapixel video camera. I like a video camera for looking at mycelium, since it's actually very active, and you can see and record videos of the cellular activity. Even spores have rapidly moving 'internals' in the minutes just before the mycelium pops out. The picture above is a still shot from a movie I took a few years ago of the spore germinating. Here's a frame from a minute or two before and one from a minute or so after the one above.

Below is another shot of a different cluster with monokaryotic mycelium emerging from several spores, taken about fifteen minutes after germination. I was hoping to get a video of the first clamp connections forming, but no dice. This one is at 100X.
https://www.shroomery.org/forums/showflat.php/Number/12841828#12841828
assuming RR also did dilution you can see that there's still too many spores for that mycelium to stay monokaryotic for more than a half hour or so, and to try to transfer something that small would require micro-manipulation
Quote:
Workman said: I always assumed that serial dilutions were necessary to produce single spore isolates. That always seemed like too much work and I never took the time to even attempt it.
I was familiar with the traditional streak method for isolating single bacteria on agar, so I tried the same thing with spores. I works pretty good most of the time and spore clumping doesn't seem to be as much of an issue as expected. You just need to transfer the isolated germination points to new plates as soon as they are visible. Otherwise they may grow and combine with nearby mycelium or barely germinated spores that may not even be visible as mycelium.
You still need a microscope to look for clamp connections in the mycelium to confirm it is monokaryotic.
Below are images of the streaking technique as used for bacteria.
no pics tho so sorry workman even tho he's workman I'll believe it when I see it. Because I sure as shit would have took some pics.
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O0ZE
Ascended Drone for the hive



Registered: 02/28/18
Posts: 321
Loc: Getting Toasted in the sun
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: bodhisatta]
#25435792 - 09/04/18 06:59 PM (5 years, 4 months ago) |
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so it's done. (3) of them looked like they had trich, green spots. tossed half the spawn from the jars that had it in it. TBH i really feel like they are all going to trich up. I believe the contamination came from weaknesses in the .3um SFD. if not just the nature of .3UM SFDs then. The environment was not semi sterile and the manufacturer suggestion was to use micropore tape over the top port if used in a non semi sterile environment. i'm thinking about using more reliable lids for agar use in the future. or just use the mircospore tap until i start seeing mass failure. those lids weren't cheap. OR the smartest thing. put the dam jars back in my fricken work space where it's semi sterile.
we'll see. QUESTION
does mycelium benefit from light cycling? if not, i'll just leave the lights on. i imagine that leaving the light on is what the grocery store button mushrooom industry does. gotta keep'em workin' and puttin' out.
--------------------
Not everything that counts is countable and not everything that is countable counts.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 11 hours
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Re: Cultivation General Discussion [Re: O0ZE] 1
#25435825 - 09/04/18 07:11 PM (5 years, 4 months ago) |
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It does benefit from a 12/12 but constant light can work
My bet is it wasnt the sfd... Most likely a combination of all 3 vectors, poor lid build, iffy inoculant/technique, and bad grain prep
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25435881 - 09/04/18 07:28 PM (5 years, 4 months ago) |
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On the AA sterilizer without a basket you dont have to tie the vent tube up, it isn't long enough to go down into the water. You can just let it dangle, i do it all the time. You can hold the lid level next to the cooker to get an idea of high far down it goes, but on mine at least it doesn't really come close to the water level.
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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Re: Cultivation General Discussion [Re: sandman420]
#25436362 - 09/04/18 10:20 PM (5 years, 4 months ago) |
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Ahh yeah bod, those were the posts I saw, back when I created my account. I spent too much time reading and no time growing back then and ended up confused af for a fat minute lol.
But yeah, I don't count that and agree, pics or it didn't happen.
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: Crispykoot]
#25436695 - 09/05/18 03:27 AM (5 years, 4 months ago) |
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Quote:
Crispykoot said:
Quote:
bodhisatta said: The AA 25x. Got it used for 200 dollars so i was wary of how well it would work right out of the box
One thing I found with my AA's is to tighten the lid down just a little before venting...Get the lid placed evenly and tight enough to stay on well but after venting when everything is nice and hot then tighten the lid down snug. New AA's will not seal perfectly for a few rounds.
My new AA921 still won't seal properly. Going to try and vent like this first and then tighten the nuts. Thanks!
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Huskies
Boop More Snoots



Registered: 03/22/16
Posts: 1,048
Last seen: 1 day, 3 hours
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BRF plates, any thoughts? I ran out of agar powder so I did a batch of these this morning.
What should I expect/know in comparison to regular pasty plates?
-------------------- I call them Huskies cause you tell them to go "Mush! Mush""
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