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Leftfield420
bong toker



Registered: 02/26/16
Posts: 10,023
Last seen: 9 hours, 21 minutes
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Re: Cultivation General Discussion [Re: NumeroEno]
#25409625 - 08/24/18 09:56 AM (5 years, 5 months ago) |
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I have an AA930 and it is almost too big for my stove because of the cabinets next(above) to the stove
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Legend21
Legend



Registered: 04/27/18
Posts: 1,148
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: Leftfield420]
#25409876 - 08/24/18 11:55 AM (5 years, 5 months ago) |
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What’s your guys call mold or no? This is transfer four about to make another transfer but I think I’m just transferring mold. I have had zero luck from swabbing spores so far
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Cultivation General Discussion [Re: Legend21] 1
#25409918 - 08/24/18 12:21 PM (5 years, 5 months ago) |
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I'd say myc. I've had to really work my AA+ cultures and keep an eye out for the slightest bit of rhizo to xfer, otherwise, they're very tomentose like that. The blue bruising in that last pic is tell tale of of leucistic and albino cultures in my experience.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: Mycolorado]
#25410091 - 08/24/18 01:52 PM (5 years, 5 months ago) |
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Quote:
Shomann said: I wouldn't spawn them. If you inoculated with LC and then had to shake a few days later and it's still not done, plus it's appearance. Shoebox material if you have to try for a spore print, otherwise I'd throw it in the compost pile.
Fuck that. He'd be more likely to get a print if he top fruits it.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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SirHuntington
Stranger


Registered: 05/03/15
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25411576 - 08/25/18 04:18 AM (5 years, 5 months ago) |
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Quote:
tryptkaloids said:
Quote:
Shomann said: I wouldn't spawn them. If you inoculated with LC and then had to shake a few days later and it's still not done, plus it's appearance. Shoebox material if you have to try for a spore print, otherwise I'd throw it in the compost pile.
Fuck that. He'd be more likely to get a print if he top fruits it.
Yea I'm thankfully passed the stage where I'm chasing for prints.
Still trying to find the baseline for what's too bacterial and what's good to spawn though.
I'm just going to chuck these ones, got some plates ready to be put to grain anyway
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Legend21
Legend



Registered: 04/27/18
Posts: 1,148
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: Mycolorado]
#25411615 - 08/25/18 04:44 AM (5 years, 5 months ago) |
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Quote:
Mycolorado said: I'd say myc. I've had to really work my AA+ cultures and keep an eye out for the slightest bit of rhizo to xfer, otherwise, they're very tomentose like that. The blue bruising in that last pic is tell tale of of leucistic and albino cultures in my experience.
Cool I was wondering why they were bruising I’ve had earlier transfers do that and I tossed them I guess on this fifth transfer I’ll try to put a plate to grains and see what happens. It’s hard to tell when it’s so tomentose. I appreciate your help!
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O0ZE
Ascended Drone for the hive



Registered: 02/28/18
Posts: 321
Loc: Getting Toasted in the sun
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: Legend21]
#25412370 - 08/25/18 11:56 AM (5 years, 5 months ago) |
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Quote:
Legend21 said: What’s your guys call mold or no? This is transfer four about to make another transfer but I think I’m just transferring mold. I have had zero luck from swabbing spores so far

what's the temp where you grow your plates? i think higher temps encourage rhyzomorphic growth. does look like fluffy mycelia to me, but the growth looks a little odd. it is absolutely all fluff. i believe ryzos is what you're looking for.
can mycelia be manipulated into producing rhyzo? i find that my rhyzo gets fluffy in cooler temps, and after it's been dormant, but between 78 - 85f for a few days i tend to see the mycelia go ryzo more. initial growth seems to alway be fluffy for me after a time in the fridge, or on a brand new transfer. my fastest colonizing jars, however go rhyzo immediately upon transfer. no idea why. genetics may play a part. rhyzo behaves differently than fluff/ moves faster and is more aesthetic, but almost all the experienced ones here say both produce the same results. right now I've got 12 jars and all 12 show both types of growth. i like having both. i keep the temp around 82-86F during the day and allow a dip to 70 at night. heating and cooling. i've also got a fan moving air around. on day 15 and nearly all jars are colonized.
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Not everything that counts is countable and not everything that is countable counts.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: O0ZE]
#25412387 - 08/25/18 12:06 PM (5 years, 5 months ago) |
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941 is considerably wider than a 921, 925 and 930. It holds 10 quart jars per layer vs the 7 jars per layer of the others.
Edit: Maybe it was 9 on bottom and 10 on top layer, it's been a minute since I ran jars in a 941.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: O0ZE] 1
#25412406 - 08/25/18 12:14 PM (5 years, 5 months ago) |
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Quote:
O0ZE said:
Quote:
Legend21 said: What’s your guys call mold or no? This is transfer four about to make another transfer but I think I’m just transferring mold. I have had zero luck from swabbing spores so far

what's the temp where you grow your plates? i think higher temps encourage rhyzomorphic growth. does look like fluffy mycelia to me, but the growth looks a little odd. it is absolutely all fluff. i believe ryzos is what you're looking for.
can mycelia be manipulated into producing rhyzo? i find that my rhyzo gets fluffy in cooler temps, and after it's been dormant, but between 78 - 85f for a few days i tend to see the mycelia go ryzo more. initial growth seems to alway be fluffy for me after a time in the fridge, or on a brand new transfer. my fastest colonizing jars, however go rhyzo immediately upon transfer. no idea why. genetics may play a part. rhyzo behaves differently than fluff/ moves faster and is more aesthetic, but almost all the experienced ones here say both produce the same results. right now I've got 12 jars and all 12 show both types of growth. i like having both. i keep the temp around 82-86F during the day and allow a dip to 70 at night. heating and cooling. i've also got a fan moving air around. on day 15 and nearly all jars are colonized.
Rhyzo can be encouraged by lower nutes. I don't think temps play a roll. Atleast i doubt its causation
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Legend21
Legend



Registered: 04/27/18
Posts: 1,148
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: O0ZE] 1
#25412554 - 08/25/18 01:21 PM (5 years, 5 months ago) |
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Quote:
O0ZE said:
Quote:
Legend21 said: What’s your guys call mold or no? This is transfer four about to make another transfer but I think I’m just transferring mold. I have had zero luck from swabbing spores so far

what's the temp where you grow your plates? i think higher temps encourage rhyzomorphic growth. does look like fluffy mycelia to me, but the growth looks a little odd. it is absolutely all fluff. i believe ryzos is what you're looking for.
can mycelia be manipulated into producing rhyzo? i find that my rhyzo gets fluffy in cooler temps, and after it's been dormant, but between 78 - 85f for a few days i tend to see the mycelia go ryzo more. initial growth seems to alway be fluffy for me after a time in the fridge, or on a brand new transfer. my fastest colonizing jars, however go rhyzo immediately upon transfer. no idea why. genetics may play a part. rhyzo behaves differently than fluff/ moves faster and is more aesthetic, but almost all the experienced ones here say both produce the same results. right now I've got 12 jars and all 12 show both types of growth. i like having both. i keep the temp around 82-86F during the day and allow a dip to 70 at night. heating and cooling. i've also got a fan moving air around. on day 15 and nearly all jars are colonized.
Temps are around 75-78 in the room with the plates. These are the only ones fluffy like that. Here’s another example same media different culture and it’s conpletley rhizo

I don’t mind if it’s not rhizo growth I just can’t tell if it’s mold or not it looks funky tomentose sucks for that reason it’s hard to spot what’s going on.
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O0ZE
Ascended Drone for the hive



Registered: 02/28/18
Posts: 321
Loc: Getting Toasted in the sun
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: Legend21]
#25413432 - 08/25/18 09:14 PM (5 years, 5 months ago) |
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yea, i tend to stay away from fuzzy as well.
why is one part of his plate fuzzy and the other is rhyzo?
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Not everything that counts is countable and not everything that is countable counts.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: O0ZE]
#25413483 - 08/25/18 09:54 PM (5 years, 5 months ago) |
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Either different genetics or contam. Im guessing the later due to how ununiform it is
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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O0ZE
Ascended Drone for the hive



Registered: 02/28/18
Posts: 321
Loc: Getting Toasted in the sun
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25414291 - 08/26/18 10:27 AM (5 years, 5 months ago) |
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so using fluff on plates is never ok unless one wants to ride on luck; all transfers should be rhyzomorphicly pure to avoid the gap/failure area that fluff creates? i'm putting that assertion to the test right now. i used mostly rhyzomorphic plates to perform all transfers, however the plates were not uniform. it's going to take me a few minutes, but i'll edit this post with some photos of my jars; i spotted something this morning that troubles me and has got me thinking about the future of this grow.
i first want to say that i didn't see this possible morph of mycelia, or contam until today. jars have been doing great growth wise, however! i question the effectiveness of my lids now, [that's got to be the vector] but at no time in previous successful grows was i ever having trouble with lids and it's only about the 5, or 6th time I've PC'd them. i'm thinking about isolating this shady jar in a little shoe box modded tub i have.
see that top fuzz that looks like polyfil? could it have something to do with availability of O2? I have a .3um SFD and a .3um layer of micropore tape over a vent above the SFD. these jars are not in a semi sterile enviroment and the tape was added about 10 days in. These jars are 15 days old.








i know many of the photos look the same, but i need to do the best i can with what i have to illustrate my concern so the answers are as close to accurate as possible.
As a side note, i'm thinking of going LC. it seems to be "what's next." How much more effort/steps and time does it require compared to agar?
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Not everything that counts is countable and not everything that is countable counts.
Edited by O0ZE (08/26/18 10:53 AM)
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: O0ZE]
#25414366 - 08/26/18 11:10 AM (5 years, 5 months ago) |
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Agar is more likely to bring success. Those jars are bacterial as fuck. Get clean spawn with agar before moving to liquids. Lc is more likely to contam than any other method other than open air
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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O0ZE
Ascended Drone for the hive



Registered: 02/28/18
Posts: 321
Loc: Getting Toasted in the sun
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25414500 - 08/26/18 12:10 PM (5 years, 5 months ago) |
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the grain was on the moist side pre and post pressure cook. no puddles, just droplets. lotta droplets. all my successful grows have had grain on the wetter side. yields and flushes were good. aseptic techniques were good. plates were not showing signs of bacteria. I've had this comment before. my tubs also don't show that telltale spotty look of bacterial grows. bacteria only hinders yield, but it won't kill a grow, or make the products toxic when dried in a dehydrator? I've not had success with inoculating dryer grain and getting full colonization. other photos will show that clear gel substance that mycelia produces in wetter environments. i'm not convinced it's bacteria. the mycelia is not producing metabolites to fight it and if the jars's moistness were directly related to bacteria, i'd have very little growth because the bacteria would have become entirely to prevalent in the jars. when i drop the temps at night the jars get a dryer appearance. bacteria can stop a jars growth dead in it's tracks and reproduces much faster then mycelia. my jars would have had zero chance if the moistness were directly tied to bacteria.
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Not everything that counts is countable and not everything that is countable counts.
Edited by O0ZE (08/26/18 12:19 PM)
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: O0ZE] 1
#25415580 - 08/26/18 09:00 PM (5 years, 5 months ago) |
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You act as if its one size first all.. There are estimated around 3.5 billion microbes, only around 200 thousand we know of.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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DreamReality
MycoPilot



Registered: 03/31/09
Posts: 477
Last seen: 3 months, 13 days
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25416011 - 08/27/18 02:44 AM (5 years, 5 months ago) |
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My first attempt at cloning. I'm starting to really enjoy agar work
Edited by DreamReality (08/27/18 03:10 AM)
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FishLevelMidnight
Aquaman



Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 25 days
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Re: Cultivation General Discussion [Re: DreamReality] 2
#25416390 - 08/27/18 08:56 AM (5 years, 5 months ago) |
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My Puerto Rican LC culture shoebox is like 4-7 days from harvest
I'll post pics in a few days once the shoebox is ready, but I wanted to just give mushboy some time to find out ways to make hats palatable

Just messing bud! Love the mushcult
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ManifoldPrime
Per Ardua Ad Astra



Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 1 month
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Re: Cultivation General Discussion [Re: DreamReality]
#25416635 - 08/27/18 11:19 AM (5 years, 5 months ago) |
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Quote:
DreamReality said:

My first attempt at cloning. I'm starting to really enjoy agar work
I started to enjoy agar work in early 2017. I fucking adore it now and do it 1-2 times a week, sometimes in stages. Both pours and no-pours are fascinating and fun to work with. Comparing medias, isolating traits like rhizomorphism, its always fun
-------------------- My Magnum Opus:Thai Variety Comparison Please correct me if I say something wrong! | Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |
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boom boom

Registered: 02/27/18
Posts: 429
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I've got 4 dishes with 4 different varieties in them that I've had sitting around for two weeks. A while to wait yet but I can't wait for them to pin because I'm getting the itch lol. It'll be fun getting clone masters from them.
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