|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Zero Nowhere
Beer Drinker



Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon
|
Re: Cultivation General Discussion [Re: myceliups]
#25201739 - 05/13/18 05:50 PM (5 years, 8 months ago) |
|
|
Mine used to turn black from damprid drying.
We met aliens the night we ate the black ones!
Edited by Zero Nowhere (05/13/18 05:52 PM)
|
sangreen
Dunce


Registered: 03/31/18
Posts: 182
Loc: Enceladus
Last seen: 1 year, 1 month
|
Re: Cultivation General Discussion [Re: Zero Nowhere]
#25202195 - 05/13/18 10:45 PM (5 years, 8 months ago) |
|
|
It's time for another stupid experiment.
I had some petris that were so overgrown, the myc was going up the wall, through the gap under the lid, and back down the other side underneath the plastic wrap. I don't have any vacant grains to transfer this escape artist, so I cased it with damp verm. It has appeared to accept its new fate and begun to colonize the verm. Let's see how far it gets before it gives up.
-------------------- I figured it out, what life is about It crawled into my brain and it's starting to sprout
|
Mycosperiment
Stranger

Registered: 11/19/17
Posts: 198
Loc: USA
Last seen: 11 months, 17 days
|
Re: Cultivation General Discussion [Re: sangreen]
#25203160 - 05/14/18 12:06 PM (5 years, 8 months ago) |
|
|
Quote:
sangreen said: It's time for another stupid experiment.
I had some petris that were so overgrown, the myc was going up the wall, through the gap under the lid, and back down the other side underneath the plastic wrap. I don't have any vacant grains to transfer this escape artist, so I cased it with damp verm. It has appeared to accept its new fate and begun to colonize the verm. Let's see how far it gets before it gives up.
Please update this thread with your results. I've considered doning the same thing but never tried it. I wouldn't expect it to get very big or produce very much but should be enough to get a small print or maybe a few small prints. Should be nicer shaped fruits than ones that grow fully inside the closed plates.
|
stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
|
Re: Cultivation General Discussion [Re: Crispykoot]
#25203206 - 05/14/18 12:26 PM (5 years, 8 months ago) |
|
|
Quote:
Crispykoot said: Mycelium may be able to transfer nutrients and water some distance but for sure it chooses to conserve energy whenever possible.
I think we use way too much light. Time and time again I see huge shrooms coming out of tubs that I have taken out of my room that are kept in a very low light environment with a short day cycle. Healthy and normal but big. I feel like there is a trigger that we don't play with a whole lot. Light cycle/intensity and temps seem to be a part of it.
This is what I was initially thinking when I posted about the mycelial network. I'm curious about the value of light (or absence) and whether mycelium can transfer that "energy" as well. Although since we don't know how or if it's using light, it's just me thinking out loud.
|
sangreen
Dunce


Registered: 03/31/18
Posts: 182
Loc: Enceladus
Last seen: 1 year, 1 month
|
|
I did this one on the 10th or 11th. There's a thin line of bruising around the edge of the dish, where I trimmed the escaping mycelium. Colonization was initially only from the edges but has started in other regions. The bag is open for the photo, but is normally kept about half zipped. Misted lightly a few hours ago.
-------------------- I figured it out, what life is about It crawled into my brain and it's starting to sprout
Edited by sangreen (05/14/18 01:01 PM)
|
Mycosperiment
Stranger

Registered: 11/19/17
Posts: 198
Loc: USA
Last seen: 11 months, 17 days
|
Re: Cultivation General Discussion [Re: sangreen]
#25203600 - 05/14/18 03:40 PM (5 years, 8 months ago) |
|
|
Quote:
sangreen said: I did this one on the 10th or 11th. There's a thin line of bruising around the edge of the dish, where I trimmed the escaping mycelium. Colonization was initially only from the edges but has started in other regions. The bag is open for the photo, but is normally kept about half zipped. Misted lightly a few hours ago.

I've been using small 47mm plates for so long I forgot what a full sized plate looks like. This experiment might do better than I was imagining. It will be interesting to see the quantity you get. I've gotten 1,2, or sometimes 3 thin stemmed small capped mushrooms in vitro in my little plates with just a thin layer of malt extract agar. They should do a lot better with more media, a casing layer, more space and more fresh air.
|
Lemgrub


Registered: 04/02/18
Posts: 589
|
|
Quote:
stareatclouds said:
Quote:
Crispykoot said: Mycelium may be able to transfer nutrients and water some distance but for sure it chooses to conserve energy whenever possible.
I think we use way too much light. Time and time again I see huge shrooms coming out of tubs that I have taken out of my room that are kept in a very low light environment with a short day cycle. Healthy and normal but big. I feel like there is a trigger that we don't play with a whole lot. Light cycle/intensity and temps seem to be a part of it.
This is what I was initially thinking when I posted about the mycelial network. I'm curious about the value of light (or absence) and whether mycelium can transfer that "energy" as well. Although since we don't know how or if it's using light, it's just me thinking out loud.
I believe the photo reaction would be constrained to each primary hyphal knot, as this is the first step that actually requires light for fruiting body development. So all the primary hyphal knots would form in the dark anyway and just be waiting for a decent light exposure to set off a signal cascade to progress in development. There was one paper that showed just 30 minutes of exposure significantly upregulated gene expression in possible genes needed for fruiting body development.
Anyway, I wouldn't think the signal cascade would travel outside the bounds of each knot.
-------------------- We getting that pirate bay, alien shishkebab cordycep money

|
thelanzii

Registered: 11/13/12
Posts: 5,434
|
Re: Cultivation General Discussion [Re: Lemgrub]
#25205688 - 05/15/18 02:37 PM (5 years, 8 months ago) |
|
|
just finished my first no pour plate transfers some things i think it went ok
some difficulties were less visibility than i would like so i think i will use a desk lamp next time right on my sab or close by
it was somewhat diffcult to see what i was transferring and if i had made a proper cut
setting the agar the on the transfer plate was a challenge for some plates as sometimes the only way i could get the cut agar out of the original plate was to stab it which made it difficult to set it in the new plate
picking the agar up on one side of the scalpel when possible made transferring a lot easier
there were a few plates that didn;t get a transfer to the exact middle of the plate which was kind of frustrating
it was a good learning experience and I will have things to improve technique wise next transfer and inoculation session
hoping i get some good plates though
|
Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
|
Re: Cultivation General Discussion [Re: thelanzii]
#25205702 - 05/15/18 02:45 PM (5 years, 8 months ago) |
|
|
Munchauzen has a vid on proper transfer technique with no-pours and I also have a vid at the end of my no-pour guide to illustrate how to work with plates upside-down and how to scoop up the wedge. Try to emulate that, and do practices with plates you're gonna trash whenever possible.
|
stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
|
Re: Cultivation General Discussion [Re: Lemgrub]
#25205705 - 05/15/18 02:46 PM (5 years, 8 months ago) |
|
|
Quote:
Lemgrub said: I believe the photo reaction would be constrained to each primary hyphal knot, as this is the first step that actually requires light for fruiting body development. So all the primary hyphal knots would form in the dark anyway and just be waiting for a decent light exposure to set off a signal cascade to progress in development. There was one paper that showed just 30 minutes of exposure significantly upregulated gene expression in possible genes needed for fruiting body development.
Anyway, I wouldn't think the signal cascade would travel outside the bounds of each knot.
That's very interesting. Thanks for the response. I'll just assume water is responsible for the bottom fruits size and perhaps they'd get bigger with light.
|
Canharo
Glassblowing Extraordinaire



Registered: 10/27/14
Posts: 327
Last seen: 7 months, 29 days
|
Re: Cultivation General Discussion [Re: thelanzii]
#25206241 - 05/15/18 07:28 PM (5 years, 8 months ago) |
|
|
Quote:
EtherealMind said: just finished my first no pour plate transfers some things i think it went ok
some difficulties were less visibility than i would like so i think i will use a desk lamp next time right on my sab or close by
it was somewhat diffcult to see what i was transferring and if i had made a proper cut
setting the agar the on the transfer plate was a challenge for some plates as sometimes the only way i could get the cut agar out of the original plate was to stab it which made it difficult to set it in the new plate
picking the agar up on one side of the scalpel when possible made transferring a lot easier
there were a few plates that didn;t get a transfer to the exact middle of the plate which was kind of frustrating
it was a good learning experience and I will have things to improve technique wise next transfer and inoculation session
hoping i get some good plates though

E Real, I'm new to agar to but got a couple hundred transfers down over last couple months, for me a big thing is how thin I pour a plate, if you pour them thin its easier to get on the sides to scoop up you wedge instead of stab it, if its real thick it can be harder to remove wedges.
If you have to stab or it gets stuck to your scalpel when your trying to put it in the reciving plate take the blade and 'cut' into the new plate, you will free your transfer doing this by cutting it off as you 'slash' the plate, I hope that helps a bit that tid bit helped me a bit on stuck transfers or plates I got a little heavy-handed with and poured thick.
Also I have 3 lights when I do transfers, a room light, one on top or over my SAB and I have a tub under my tub as a table with a light pointing up I can flip on if need be, I like to draw on the back of my petri where im going to take transfers and can see the marker threw the sab a little easier then just the leading edge, between the lights and my marker guide lines its much easier for me. Only thing that sucks about not getting them in the center is you'll have to transfer sooner but in the beginning you'll want to transfer asap anyway, I'll put 3 transfers on one plate to save plates sometimes so you can use it to ypur advantage too. Hope any of this helps
|
misterswisher
Stranger
Registered: 04/07/18
Posts: 266
Last seen: 5 years, 18 days
|
Re: Cultivation General Discussion [Re: Canharo]
#25206542 - 05/15/18 10:23 PM (5 years, 8 months ago) |
|
|
|
Leftfield420
bong toker



Registered: 02/26/16
Posts: 10,023
Last seen: 9 hours, 36 minutes
|
|
What type of pre filters do you guys use on your hoods? I'm looking at one that is washable and has a lifetime guarantee for $40-$60 depending on brand
|
thelanzii

Registered: 11/13/12
Posts: 5,434
|
Re: Cultivation General Discussion [Re: Leftfield420]
#25208115 - 05/16/18 02:08 PM (5 years, 8 months ago) |
|
|
made some sfd lids today
an old spore syringe worked great for applying the silicone
i lost the tip that came with the tube
been wanting sfd lids for a while and finally made some
|
RomeoPapa
Jackass of All Trades



Registered: 05/14/17
Posts: 1,583
Loc: In the middle
Last seen: 1 year, 2 months
|
Re: Cultivation General Discussion [Re: thelanzii]
#25208534 - 05/16/18 05:19 PM (5 years, 8 months ago) |
|
|
After switching to sfd and plastic lids I won't ever go back to stuffing poly in metal lids again. What were you using before EM?
-------------------- It's better to have it and not need it Than it is to need it and not have it.
|
Canharo
Glassblowing Extraordinaire



Registered: 10/27/14
Posts: 327
Last seen: 7 months, 29 days
|
Re: Cultivation General Discussion [Re: RomeoPapa]
#25208558 - 05/16/18 05:26 PM (5 years, 8 months ago) |
|
|
I really got to get some of those plastic lids, seem like they'd make things a bit easier, but I'm also pretty used to my two piece metal lids I can get at my local market. I got to order some stuff anyway online though so I'll probley get a couple
|
thelanzii

Registered: 11/13/12
Posts: 5,434
|
Re: Cultivation General Discussion [Re: RomeoPapa]
#25208590 - 05/16/18 05:43 PM (5 years, 8 months ago) |
|
|
ez-felt lids
after reading a post by 13shrooms on why he only uses sfds i had to make the switch
want to rule out any variables
|
RomeoPapa
Jackass of All Trades



Registered: 05/14/17
Posts: 1,583
Loc: In the middle
Last seen: 1 year, 2 months
|
Re: Cultivation General Discussion [Re: thelanzii]
#25208691 - 05/16/18 06:31 PM (5 years, 8 months ago) |
|
|
I get my ball plastic lids at super target. Way cheaper there than any place I found online. The biggest pluses for them is they don't rust and they are one piece.
-------------------- It's better to have it and not need it Than it is to need it and not have it.
|
Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 12 days, 2 hours
|
Re: Cultivation General Discussion [Re: RomeoPapa]
#25208715 - 05/16/18 06:47 PM (5 years, 8 months ago) |
|
|
Quote:
RomeoPapa said: I get my ball plastic lids at super target. Way cheaper there than any place I found online. The biggest pluses for them is they don't rust and they are one piece.
I agree wholeheartedly. I just did a bunch of G2G tonight with the 2 piece metal lids. Do not like. Makes me nervous getting my hands near the threads when I have to pop the lid off.
|
Canharo
Glassblowing Extraordinaire



Registered: 10/27/14
Posts: 327
Last seen: 7 months, 29 days
|
Re: Cultivation General Discussion [Re: Asura]
#25208812 - 05/16/18 07:28 PM (5 years, 8 months ago) |
|
|
Yeah I checked the three big box stores near my home and work place and nothing, they do have a surprising amount of other accessories for jars and food preserving but no plastic lids.
Thats my typical luck when I shop places like that and after going all around and wasting my time for nothing I'd rather just go online then go back to those places
|
|