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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Cultivation General Discussion [Re: mushboy] 1
#25179787 - 05/02/18 10:44 AM (5 years, 8 months ago) |
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Quote:
mushboy said: the first step is not using bold larg ass font
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 14 hours
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Re: Cultivation General Discussion [Re: boom boom]
#25179878 - 05/02/18 11:59 AM (5 years, 8 months ago) |
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what is with all these people using large text for every word? Its an eyesore
Quote:
Josex said:Quote:
boom boom said:
Quote:
Josex said: It's not easy to get that metal piece, it's a female or male whatever that I can't remember the name right now. I don't like that it's exposed to open air even though it has that plastic thingy screwed on it. You don't have to worry about screwing on the needles, that's hardly a vector.
I did something similar here.
It's nice to get your LC into syringes but might not be worth the hassle when you can just pour.
Yeah, I don't really trust it to keep contams out over the long term. That's why I'm envisioning it more for LI than LC. You could take it straight out of the pc to the glovebox once cooled, toss in a big wedge, shake it up, and then start filling syringes. Whole process would take no time.
I just really don't like the idea of pouring. I know it works well for a lot of people, but I just see myself bumping the roof of my SAB and dumping contams into open containers, ruining all my inoculant. I'm making a bunch of one piece plastic SFD lids, and I feel great about my sterile technique with those and a syringe. I'd barely have to open the lid, and I'd never risk putting something that isn't sterile over the receiving jar. Plus they're easy to measure. If you've got a 60 ml syringe filled up, you can plan to put 2ml in 30 jars, and do it. With pouring it's gonna be way more guesswork.
As far as finding that metal piece, yeah that might be tricky. The pic is a jar that was definitely being sold at some point, but dude must have stopped making them because I couldn't find it for sale. Wouldn't mind making my own.
Only things I'm not liking about this idea are that in my experience, plastic lids don't seal fully, so I don't know how a jar like that would respond to being violently shaken. The other, which is more about LI's in general, is that idk how well I could trust mycelium to be evenly distributed throughout it. It'd suck to plan around using x amount of grain jars and then finding out that a bunch of them grew nothing because all you injected into them was sterile water.
Plastic lids will leak
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25179884 - 05/02/18 12:05 PM (5 years, 8 months ago) |
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The only way you could prevent plastic lids from leaking is with some plumbers tape i guess.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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Kyshroomer19xx
Est 19xx soldier



Registered: 04/22/17
Posts: 1,308
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Re: Cultivation General Discussion [Re: JHOVA]
#25179931 - 05/02/18 12:47 PM (5 years, 8 months ago) |
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How do i know if my grow bags are pc able
-------------------- RIP tom petty Of course it is happening inside your head, but why on earth should that mean it is not real? Albus Dumbledore
Edited by Kyshroomer19xx (05/02/18 12:50 PM)
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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They are sold for mushroom spawn. Those look like the right bags though.
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Kyshroomer19xx
Est 19xx soldier



Registered: 04/22/17
Posts: 1,308
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Last seen: 4 years, 6 months
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Re: Cultivation General Discussion [Re: hamloaf]
#25179947 - 05/02/18 12:58 PM (5 years, 8 months ago) |
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Ok cool any tips you could give me this will be my first time pcing bags
-------------------- RIP tom petty Of course it is happening inside your head, but why on earth should that mean it is not real? Albus Dumbledore
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: hamloaf]
#25179950 - 05/02/18 01:00 PM (5 years, 8 months ago) |
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You can buy silicone rings that make plastic lids water tight on ebay and amazon. Wide mouth or regular size are available.
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Adaephon17
Stranger


Registered: 05/02/18
Posts: 5
Last seen: 3 years, 4 months
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Re: Cultivation General Discussion [Re: sandman420]
#25179991 - 05/02/18 01:21 PM (5 years, 8 months ago) |
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Good morning everyone! I'm a long time lurker and this will be my first post. So I'm sure like most it could be done better. That being said I'm stoked to finally pop in and join the community here. My over anxious mind is just looking for feedback on my first tubs. I assume this is doing well but concerned my sub may have had too much moisture. Just looking for some experienced input, and thank you all for having got me this far.
Edited by Adaephon17 (05/02/18 01:21 PM)
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
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Re: Cultivation General Discussion [Re: sandman420]
#25180004 - 05/02/18 01:30 PM (5 years, 8 months ago) |
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Quote:
JHOVA said: The only way you could prevent plastic lids from leaking is with some plumbers tape i guess.
Quote:
sandman420 said: You can buy silicone rings that make plastic lids water tight on ebay and amazon. Wide mouth or regular size are available.
Yes, but they suck. Putting silicone in the lid would work better
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Quote:
XxSkittlez420xX said: i just got my pictures to upload finally on here so here's some of the recent albino penis envy pic's that i have taken. ALSO! can anyone tell me how to clone these bad boys? plz? lol
    
   
APE don't drop spores like regular cubes do. A spore print wouldn't be cloning, either way. To clone, tear apart the mushroom in a SAB, and following standarding sterile technique, scrape a piece of the inner tissue with your blade and place it in agar.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Quote:
Kyshroomer19xx said: Ok cool any tips you could give me this will be my first time pcing bags
I mean, yes. All kinds of tips. Do you have an idea, or a plan on which method you are going to use to PC, and inoculate the bags? What kind of PC are you working with? I need context in order to be able to give you any tips.
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XxSkittlez420xX


Registered: 04/21/18
Posts: 37
Loc: All Around New England, U...
Last seen: 4 years, 4 months
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Re: Cultivation General Discussion [Re: mushboy]
#25180108 - 05/02/18 02:37 PM (5 years, 8 months ago) |
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im going to buy the agar solution and the dishes and stuff to clone but what is SAB? idk that abbreviation.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Quote:
XxSkittlez420xX said: im going to buy the agar solution and the dishes and stuff to clone but what is SAB? idk that abbreviation.
Still Air Box. It's almost always a big, clear storage tote with arm holes cut in. It allows you to do "sterile" work because it protects the environment from falling debris and drafts (which kick up debris). Everyone on here will either use this or a LFH (laminar flow hood) which is basically a HEPA filter and fan blowing out clean air for you to work in. A SAB is cheaper and easier to get so most have that.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Adaephon17]
#25180182 - 05/02/18 03:20 PM (5 years, 8 months ago) |
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Quote:
Adaephon17 said:

looks decent. dont mist or anything. just let it work
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boom boom

Registered: 02/27/18
Posts: 429
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25180220 - 05/02/18 03:38 PM (5 years, 8 months ago) |
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Quote:
tryptkaloids said: Yes, but they suck. Putting silicone in the lid would work better
Can you explain what you mean? I'm also thinking that wrapping the lid in saran wrap after the transfer and before the shake would probably do the trick.
I did some browsing and I think I discovered a way to do this for very cheap. This thing is a female luer bulkhead to barb. It's available from a few different sources. Costs about $25 for 20 of them, or you could get one nice one in stainless steel for the same price.


 The left side is a female luer. A syringe can screw directly onto it. The middle is a threaded section. If I cut a hole in the lid the exact size of the diameter of the threads, I can pinch the lid between the nut and the hex section. Washers and silicon could be used to create a tight fit. The right side is a barb where a hose can be inserted. Autoclaveable hosing runs about $1 a foot. It would take about 6 inches for each jar. You could seal that with a hose clamp, silicon, or maybe loctite.
I'll probably give it a shot. So long as everything is polypropylene I imagine you'd get quite a bit of life out of a lid. You'd also need to silicon on a whatman filter obviously. And you'd need a female luer cap to keep it covered until you attach the syringe. Or maybe you could attach the syringe and pc the whole assembly lol. Now that's a sterile transfer.
Edited by boom boom (05/02/18 03:56 PM)
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: boom boom]
#25180250 - 05/02/18 03:57 PM (5 years, 8 months ago) |
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25180335 - 05/02/18 04:44 PM (5 years, 8 months ago) |
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Quote:
tryptkaloids said:
Quote:
JHOVA said: The only way you could prevent plastic lids from leaking is with some plumbers tape i guess.
Quote:
sandman420 said: You can buy silicone rings that make plastic lids water tight on ebay and amazon. Wide mouth or regular size are available.
Yes, but they suck. Putting silicone in the lid would work better
What? Silicone rings work perfectly fine, never had a single problem from them and I've used them for many years. Seals plastic lids perfectly and reusable forever. The ones that suck are the thin red Tattler silicone rings, those are fucking garbage and fall in the jar. The thick clear silicone rings stick to the underside of the lid when you open them and are great.
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Botis
Stranger


Registered: 02/04/18
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Re: Cultivation General Discussion [Re: sandman420]
#25180445 - 05/02/18 05:51 PM (5 years, 8 months ago) |
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Anybody else use something like this for cloning? This is a 4" surgical suture tying forceps with 3mm tips. I happened upon them while picking up some new scalpel handles. I struggled the first three times I took clones with the scalpel, both getting a suitable piece of material on the blade, as well as getting it off onto the plate. These forceps made it a breeze! I used the scalpel to make 4 cuts against the grain of the inner stem, then used these to grab a tiny sliver. Very easy to get onto the plate as well, as I just poked whichever tip the material was stuck to into the agar and gave a little twist.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: Botis]
#25180461 - 05/02/18 05:58 PM (5 years, 8 months ago) |
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I don't use, or have yet to use those for taking inner-tissue sample cloning material. I have used those to pick individual, colonized kernels of grain, and transfer them onto Petri dishes with agar to get a culture going, and or to save a culture that I've forgotten to otherwise make culture from agar out of. May have to give it a shot.
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Botis
Stranger


Registered: 02/04/18
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Re: Cultivation General Discussion [Re: hamloaf]
#25180489 - 05/02/18 06:16 PM (5 years, 8 months ago) |
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These are gonna be useful. I'm gonna need to do just that with some stubborn APE spores that I got to germ on some RGS in an invitro pod, after failing with 12 plates so far.
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