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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 11 hours, 55 minutes
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Re: Cultivation General Discussion [Re: Thufir_Hawat] 1
#25164774 - 04/25/18 03:30 PM (5 years, 9 months ago) |
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Nah I do a constant harvest actoss all my shoeboxes, I pick em as they pop
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Doc9151
Mycologist



Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
Last seen: 1 year, 6 months
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Re: Cultivation General Discussion [Re: Failboat]
#25164776 - 04/25/18 03:32 PM (5 years, 9 months ago) |
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--------------------
  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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topsykretts
Stranger


Registered: 07/18/14
Posts: 384
Last seen: 1 year, 6 months
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Re: Cultivation General Discussion [Re: Failboat]
#25164809 - 04/25/18 03:44 PM (5 years, 9 months ago) |
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I took a biopsy from an agar plate that I inoculated with MS.. I had two plates, they were both the same age, and one of them fruited in the plate. I took the sample from the one that did not fruit and made a liquid culture from that.
Did I screw up by not taking it from the one that did fruit? I've already got a monotub that I just cased with verm 3 days ago and put into fruiting conditions.. the mycelium all looked aerial both in the jars and in the tub. Also got a shoebox that I can monitor. I don't see any knots yet.. am I just being impatient or did I screw up by using the plate that did not fruit to make my LC?
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 11 hours, 55 minutes
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Re: Cultivation General Discussion [Re: topsykretts]
#25164824 - 04/25/18 03:49 PM (5 years, 9 months ago) |
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I don't trust LC regardless. In your case, as long as the samples were clean, aaand your sterility in par, then you're gonna be fine.
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Thufir_Hawat
Mentat


Registered: 03/18/17
Posts: 399
Last seen: 4 days, 8 hours
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Re: Cultivation General Discussion [Re: Failboat]
#25164827 - 04/25/18 03:50 PM (5 years, 9 months ago) |
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Quote:
Quirkmeister92 said: Nah I do a constant harvest actoss all my shoeboxes, I pick em as they pop
Sweet, I'd been wondering about this but was having trouble coming up with the right search terms. Now to try and figure out what is keeping these fruits from gettin big... Already one surprise in this tub, apparently the spores that germinated were missing the leucism in the fruits the print came from.
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 11 hours, 55 minutes
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Re: Cultivation General Discussion [Re: Thufir_Hawat]
#25164836 - 04/25/18 03:54 PM (5 years, 9 months ago) |
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Fruit size is all genetic, and until you have an isolate the sizes will vary.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: Failboat]
#25165146 - 04/25/18 05:52 PM (5 years, 9 months ago) |
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Genetics are most important, but other conditions also play a part in fruit size. Even with isolates, you could experience variance in size between tubs with different substrate moisture levels. Fruits are like 90% water and size has to come from somewhere. But the genetics probably cap the size under optimal conditions,, so yeah.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Correct. Substrates either being too dry, or too wet will affect fruit body size. Genetics play main role in determining fruit body size in correlation to optimal conditions.
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Thufir_Hawat
Mentat


Registered: 03/18/17
Posts: 399
Last seen: 4 days, 8 hours
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Re: Cultivation General Discussion [Re: hamloaf]
#25165232 - 04/25/18 06:21 PM (5 years, 9 months ago) |
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Yeah that's my understanding, genetics are the limits, conditions are the... conditions.
14g bioassay underway for these lil misfits, had to pick a couple partners for the earlybird
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
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Re: Cultivation General Discussion [Re: Thufir_Hawat]
#25165289 - 04/25/18 06:47 PM (5 years, 9 months ago) |
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Use poop.
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Shadowboxing the apocalypse and wandering the land
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Lemgrub


Registered: 04/02/18
Posts: 589
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Quote:
fishermansjc said:
Quote:
Lemgrub said: And to those having trouble germinating spores, I've been doing some studying on that topic among the agarics.
Some strategies that may be worth a try are heat shocking at ~45℃ for at least an hour, using oat soak agar, or placing the agar container somewhere enclosed close to a lot of colonizing myc. A paper on amanita showed that a 7C compound exuded by live amanita myc induced germination in other amanita spores.
For the agar another paper showed that minimal media was more successful than enriched, so if trying the oat soak agar make sure it's not too nutritious.
I would be careful making assumptions from different genera, especially if there was not a mechanism proposed or identified in the papers IMHO
Correct. That's why I typed that they may be worth a try, not that they were shown to work for cubensis. Writing a disclaimer every time I make a suggestion is tedious. However if enough people try it and post the data then we could observe trends and start applying new techniques to cultivation.
I have some confidence that if a paper covers a closely related family/species with a similar strategy (coprophile) then it has a good chance to apply to cubes. I realize Amanita is ectomycorrhizal.
http://mic.microbiologyresearch.org/content/journal/micro/10.1099/00221287-77-2-393 https://en.wikipedia.org/wiki/Coprinopsis_radiata#cite_note-Mills_1973-6
there were another few I can't find the links for right now
-------------------- We getting that pirate bay, alien shishkebab cordycep money

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Thufir_Hawat
Mentat


Registered: 03/18/17
Posts: 399
Last seen: 4 days, 8 hours
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Re: Cultivation General Discussion [Re: Thufir_Hawat]
#25165817 - 04/25/18 10:50 PM (5 years, 9 months ago) |
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Quote:
Thufir_Hawat said: 14g bioassay underway for these lil misfits, had to pick a couple partners for the earlybird

Thoroughly grabbed by the boo-boo
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 11 hours, 55 minutes
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Re: Cultivation General Discussion [Re: Thufir_Hawat]
#25166683 - 04/26/18 11:58 AM (5 years, 9 months ago) |
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 Hidden contam. Shook it and then there it was.
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pacmanbreed



Registered: 10/12/16
Posts: 3,659
Last seen: 1 year, 11 months
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Re: Cultivation General Discussion [Re: Inocuole]
#25166707 - 04/26/18 12:06 PM (5 years, 9 months ago) |
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Hoping its not off topic. I was so happy i found this sacred cactus thrown away beside the road.

I guess its a calling to be around ethno forum and dig deeper for a while.
Sp much gratitude to brothers around mush cultivation for my 1st mush successful grow.
Edited by pacmanbreed (04/26/18 12:07 PM)
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
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Re: Cultivation General Discussion [Re: pacmanbreed]
#25166711 - 04/26/18 12:08 PM (5 years, 9 months ago) |
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Thrown away?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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pacmanbreed



Registered: 10/12/16
Posts: 3,659
Last seen: 1 year, 11 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25166718 - 04/26/18 12:11 PM (5 years, 9 months ago) |
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Yes brother tryp. I loaded them on the way home.
Im not sure though if its a sacred san pedro or if it contains mescaline.
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Thedenthead



Registered: 04/19/16
Posts: 531
Last seen: 4 months, 27 days
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Re: Cultivation General Discussion [Re: pacmanbreed]
#25166724 - 04/26/18 12:15 PM (5 years, 9 months ago) |
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Did a simmer / no soak prep of wbs for te first time. I’ve always just soaked overnight before to prep. Anyways, followed hamloafs tek, but my wbs came out way stickier and clumpier than ever. The wbs I’m using does have cracked corn so I’m assuming that’s the culprit. Regardless, I got 7 jars pc’d and nocced em up with wedges. Hoping it wasn’t a waste and a failed venture to try a new prep.
-------------------- Nothing but my meandering thoughts.
An ounce of prevention is worth a pound of cure.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: pacmanbreed]
#25166725 - 04/26/18 12:15 PM (5 years, 9 months ago) |
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Thats a cereus sp. Non psycboactive
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Marmie
Peter peter portion eater



Registered: 10/21/15
Posts: 918
Loc: Deep inside the night
Last seen: 3 months, 20 days
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Re: Cultivation General Discussion [Re: pacmanbreed]
#25166735 - 04/26/18 12:18 PM (5 years, 9 months ago) |
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Thats not a trich n doesnt contain mesc, sry bro. Its a nice cactus tho
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 11 hours, 55 minutes
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Re: Cultivation General Discussion [Re: Thedenthead]
#25166741 - 04/26/18 12:20 PM (5 years, 9 months ago) |
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Quote:
Thedenthead said: Did a simmer / no soak prep of wbs for te first time. I’ve always just soaked overnight before to prep. Anyways, followed hamloafs tek, but my wbs came out way stickier and clumpier than ever. The wbs I’m using does have cracked corn so I’m assuming that’s the culprit. Regardless, I got 7 jars pc’d and nocced em up with wedges. Hoping it wasn’t a waste and a failed venture to try a new prep.
I have found that cracked corn when simmered gums the whole jar up. If only soaked, then I am rather pleased with the results. In fact I'm gonna prep some meow. Gotta get them sunflower seeds out though, IME the cracked corn wbs sans SF is quite good. Gonna make masters with it to G2G into me oats
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