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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: JHOVA] 1
#25160767 - 04/23/18 07:39 PM (5 years, 9 months ago) |
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boom boom

Registered: 02/27/18
Posts: 429
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Re: Cultivation General Discussion [Re: JHOVA]
#25160880 - 04/23/18 08:18 PM (5 years, 9 months ago) |
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Quote:
JHOVA said: You need to look up josex โno mercy for bacteriaโ and post a video of your sab work. Follow both steps and you wont be a mold farmer
Inoculated 4/11, opened today. MS drop to scalpel to agar.

I've had dozens of plates exactly like this one. The only blemish on the surface is where my syringe/scalpel touched the agar nearly two weeks ago, and I usually let them sit for 3 before I say fuck it and trash them. Smells like malt extract. No liquid or greasiness. No visible growth. Say what you will about sterile technique, but the fact is that mycelium didn't even begin to grow on that plate, or any of the ones like it, when a live culture introduced to it almost certainly would've colonized them. Spores are just bullshit. It's like I'm trying to breed pandas or some shit.
That's probably why there's mushrooms that are thousands of years old. It can take that long for some spores to land in an environment they can germinate in. If the fungus died after the first thousand years, odds are it wouldn't have reproduced, and the species would go extinct. Makes ya think.
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Tight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 4 months, 8 days
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Re: Cultivation General Discussion [Re: bodhisatta]
#25160895 - 04/23/18 08:23 PM (5 years, 9 months ago) |
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it isn't working
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: boom boom]
#25160922 - 04/23/18 08:34 PM (5 years, 9 months ago) |
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I canโt make anything from that pic sorry 
Maybe the syringe is bunk? If you try the josex method many people have used it specifically for 1. Stubborn spores And 2. For cleaning dirty shit. I wouldnt recommend it if i didnt think it would help you. Take that as you will.
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boom boom

Registered: 02/27/18
Posts: 429
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Re: Cultivation General Discussion [Re: JHOVA]
#25160976 - 04/23/18 09:00 PM (5 years, 9 months ago) |
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Quote:
JHOVA said: I canโt make anything from that pic sorry 
Maybe the syringe is bunk? If you try the josex method many people have used it specifically for 1. Stubborn spores And 2. For cleaning dirty shit. I wouldnt recommend it if i didnt think it would help you. Take that as you will.
There's nothing to see really. It's just a clean plate of agar. I've got 5 syringes I haven't even tested yet so of it is a syringe issue, surely one of these has got to be viable.
And yeah I've seen that about brf, and I think I read that tek a while ago, tho I appreciate the suggestion. I'll give it a go. Maybe I'll finally get some luck.
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Canharo
Glassblowing Extraordinaire



Registered: 10/27/14
Posts: 327
Last seen: 7 months, 29 days
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Re: Cultivation General Discussion [Re: myceliups]
#25161045 - 04/23/18 09:24 PM (5 years, 9 months ago) |
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Quote:
myceliups said:
Quote:
Canharo said:
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myceliups said: Redboy pin transferred to agar.

Sweeeet Myceliups, I transferred my first RB pin the other day, taking it to as many plates as I can in a little less then an hour. I'm about to pour up plates now to transfer the new growth from the RB pin & some for a few m.s. Pan Jam.'s. I got 6 apes on wbs with more soaking now to do 6 more A2G transfers tomorrow, happy to be busy 
Good stuff!!  Have you grown redboy before?
No I recently acquired a print threw Mr.Loaf and have had some issues getting a clean looking culture, no obvious contamination but just looked off. One of the cleanist looking plates put out this little guy so I put him to agar and tranffered all the new growth to 8 more petris last night
 The plate I took it from is forming knots like crazy now should be fun to watch, Ive got a good feeling about the RB now as I'm starting to feel comfortable with my agar work
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FishLevelMidnight
Aquaman



Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 25 days
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Re: Cultivation General Discussion [Re: Canharo]
#25161170 - 04/23/18 10:03 PM (5 years, 9 months ago) |
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I donโt want to spend time writing a โTEKโ if itโs common knowledge, but has anyone else done the following with stubborn spores?
Add spores to sterile swab (or from a stubborn swab like PE or APE). With clean scalpel, cut a small well into the center of the agar dish. With a clean pair of scissors, cut the head of the swab into the center of the dish, within the well. Soak with sterile water.
Let sit and germinatie. I did this with a PE swab from germs that wouldnโt germinate after 3 attempts, I got germination now, a T1 growing out and a fuzzy little tip I can roll around the plate and inoculate with. Going to try with super old R44 spores from a print and see how reproducible it is.
Anyone ever do something similar?
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Never heard of that approach, but it sounds viable. Dried out spores are always a stubborn creature to get to germinate. Another, popular, old school method of rehydrating spores is to suspend spores into solution, draw spore solution into syringes, them wrap syringe with a rubber band. https://www.shroomery.org/forums/showflat.php/Number/15383615#15383615
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: boom boom]
#25161455 - 04/24/18 01:49 AM (5 years, 9 months ago) |
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Quote:
boom boom said:
Quote:
JHOVA said: You need to look up josex โno mercy for bacteriaโ and post a video of your sab work. Follow both steps and you wont be a mold farmer
Inoculated 4/11, opened today. MS drop to scalpel to agar.

I've had dozens of plates exactly like this one. The only blemish on the surface is where my syringe/scalpel touched the agar nearly two weeks ago, and I usually let them sit for 3 before I say fuck it and trash them. Smells like malt extract. No liquid or greasiness. No visible growth. Say what you will about sterile technique, but the fact is that mycelium didn't even begin to grow on that plate, or any of the ones like it, when a live culture introduced to it almost certainly would've colonized them. Spores are just bullshit. It's like I'm trying to breed pandas or some shit.
That's probably why there's mushrooms that are thousands of years old. It can take that long for some spores to land in an environment they can germinate in. If the fungus died after the first thousand years, odds are it wouldn't have reproduced, and the species would go extinct. Makes ya think.
Looks like that syringe is fucked with mold (pics you posted before), unless that syringe is really important to you, I'd get a print and put it to agar, they give way less trouble than spore syringes. If that syringe is all you got, try squirting a drop or two on pf tek substrate, you don't need an actual cake for that, a smallish little puck will do.
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MooseShroom
Wandering Soul


Registered: 05/24/17
Posts: 332
Last seen: 8 days, 4 hours
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Re: Cultivation General Discussion [Re: Josex]
#25161469 - 04/24/18 02:01 AM (5 years, 9 months ago) |
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How often do you guys replace your scalpel blades? I just noticed one of mine was bent, which explained some of my wonky transfers.
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Canharo
Glassblowing Extraordinaire



Registered: 10/27/14
Posts: 327
Last seen: 7 months, 29 days
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Re: Cultivation General Discussion [Re: MooseShroom]
#25161528 - 04/24/18 03:24 AM (5 years, 9 months ago) |
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Quote:
MooseShroom said: How often do you guys replace your scalpel blades? I just noticed one of mine was bent, which explained some of my wonky transfers.
They are cheap enough I replace them every SAB session and I keep two ready at a time, I buy 400+ at a time and they come presterlized in individual packaging for cheap with a new handle. The repeated heating really starts to dull the blade after a long session, I've used them more then that but they are cheap and degrade quick
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: MooseShroom]
#25161664 - 04/24/18 06:10 AM (5 years, 9 months ago) |
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Quote:
MooseShroom said: How often do you guys replace your scalpel blades? I just noticed one of mine was bent, which explained some of my wonky transfers.
Depends on work load. 1 or twice a month seems reasonable. If I'm going transfer crazy maybe once a week.
Whenever it's dull, cracked, chipped or bent.
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Leftfield420
bong toker



Registered: 02/26/16
Posts: 10,023
Last seen: 7 hours, 15 minutes
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Re: Cultivation General Discussion [Re: mushboy]
#25161680 - 04/24/18 06:20 AM (5 years, 9 months ago) |
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I change mine out before each session...but only because I have like 200 blades
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boom boom

Registered: 02/27/18
Posts: 429
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Re: Cultivation General Discussion [Re: Leftfield420]
#25162101 - 04/24/18 11:04 AM (5 years, 9 months ago) |
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Quote:
Josex said: Looks like that syringe is fucked with mold (pics you posted before), unless that syringe is really important to you, I'd get a print and put it to agar, they give way less trouble than spore syringes. If that syringe is all you got, try squirting a drop or two on pf tek substrate, you don't need an actual cake for that, a smallish little puck will do.
My first syringe was definitely junk, and the second isn't looking a whole lot better. I've got 6 syringes right now, 4 with the caps still on, and I'm gonna nocc up 6 pucks with them. One puck each. We'll see how the syringes respond. I used SFD lids to try and provide enough GE for pinning if I can get one to colonize. And I know for my personal collection I'm going to deal exclusively with inoculation loops and spore prints, for sure.
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: boom boom]
#25162113 - 04/24/18 11:09 AM (5 years, 9 months ago) |
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if you still get mold/bacteria look into your technique. No vendor can be that bad i hope
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: boom boom]
#25162118 - 04/24/18 11:12 AM (5 years, 9 months ago) |
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Quote:
boom boom said:
Quote:
Josex said: Looks like that syringe is fucked with mold (pics you posted before), unless that syringe is really important to you, I'd get a print and put it to agar, they give way less trouble than spore syringes. If that syringe is all you got, try squirting a drop or two on pf tek substrate, you don't need an actual cake for that, a smallish little puck will do.
My first syringe was definitely junk, and the second isn't looking a whole lot better. I've got 6 syringes right now, 4 with the caps still on, and I'm gonna nocc up 6 pucks with them. One puck each. We'll see how the syringes respond. I used SFD lids to try and provide enough GE for pinning if I can get one to colonize. And I know for my personal collection I'm going to deal exclusively with inoculation loops and spore prints, for sure.
I don't like those needles spore syringes from vendor come with, very thick. They sell them with those needles so that noobs can waste a good amount of solution. Get 21G needles for that second syringe that doesn't seem to be fkd up with mold and switch it up.
MS drop to scalpel to agar doesn't sound pretty to me. After flaming that thick needle, the first 2 drops are as good as dead, more so if you also flamed the scalpel (which you should). I'd use the 21G needle and squirt some drops into some PF pucks.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: JHOVA]
#25162121 - 04/24/18 11:15 AM (5 years, 9 months ago) |
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Quote:
JHOVA said: if you still get mold/bacteria look into your technique. No vendor can be that bad i hope
Yeah but he didn't seem to be getting satellites from technique and didn't seem to be having the same mold issues with the second syringe. I want to give him a vote of confidence and say the first syringe was just fucked, not the first time I hear people getting bad sryinges from vendors.
Edited by Josex (04/24/18 11:25 AM)
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boom boom

Registered: 02/27/18
Posts: 429
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Re: Cultivation General Discussion [Re: Josex]
#25162324 - 04/24/18 12:42 PM (5 years, 9 months ago) |
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I've definitely got some work to do on my sterile technique yet, but I'm planning on getting a lot of practice doing transfers and cleaning up cultures that I don't care about once I get some mycelium. Then I can get some good feedback. With a spore syringe it's like ok that's bacteria. Is it my sterile technique or was my technique good and the bacteria was in the syringe? You don't know. Even with satellite colonies, it could've been from the syringe water moving to the side of the jar and leaving contams behind in a streak.
As far as ms to scalpel to agar, yeah it was sketchy. Not ideal but fuck it, nothing else was working. Not something I intend to try again if I don't have to, but I wanted to see if removing some of the water from the equation helped germination by keeping things a bit more together.
Thanks for the help guys. I'll give these pucks a couple weeks to see what happens. Would've filmed it JHOVA but I was in a bit of a rush.
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TheBlackCat
Possibly Human



Registered: 10/05/16
Posts: 2,819
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: boom boom]
#25163141 - 04/24/18 07:22 PM (5 years, 9 months ago) |
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Seems like my mushies look great and the pinsets are ok but they are all short and stubby. Clearly something went wrong somewhere. I'm thinking it's partially genetics and partially a humidity issue. I'm really sure it's not contamination. They are TOC's. What do you guys think?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: TheBlackCat]
#25163149 - 04/24/18 07:24 PM (5 years, 9 months ago) |
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kinda dry. light colored caps.. side pins?? id say dry.
id bottom water. maybe 1/4 cup water
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