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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: ComebackKid]
    #23835212 - 11/15/16 11:36 AM (7 years, 2 months ago)

Well you need to wait another couple days for that to grow out some more to get a bigger wedge you could make four jars on one plate or do an li


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: ComebackKid]
    #23835224 - 11/15/16 11:39 AM (7 years, 2 months ago)

Comeback, you got some pics of clean agar that's ready to be used to inoculate?

I just haven't seen enough agar to make an educated guess.



Oh shit.

I have two plates of this transfer 2 GT. Both look equally as clean. I'll use one for my first ever liquid inoculate, and take transfers from the other.

Is there any point to do say 10 transfers? If I keep transferring the hell out of it, am I gonna get "more" or "better" results?

Wait a few more days huh. Okay I can do that. I prefer to do my PC runs and stuff on the weekend so my wife can watch little man. I take up the whole kitchen for a whole day.


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KSSS And PE WBS.
 


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Offlineenlightenment
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23835236 - 11/15/16 11:44 AM (7 years, 2 months ago)

Quote:

Moabfighter said:
Is there any point to do say 10 transfers? If I keep transferring the hell out of it, am I gonna get "more" or "better" results?





Was it a MS grow or clone?
If MS: No not better results. You may get an isolate if you make a lot of transfers but you never know how it performs until you test it. If it is crap you wasted a lot of plates...


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OfflineNDStepp84
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Re: Cultivation General Discussion [Re: Moabfighter] * 1
    #23835243 - 11/15/16 11:45 AM (7 years, 2 months ago)

Rubber side down if you don't tighten them too tight before PC'ing they aren't bad. You can always find one part of the lid that sticks out more on one side after removing the ring, holding the jar and pushing up with light pressure with the tip of your thumb and holding the pressure for a sec usually breaks the seal without the lid flying off or any problems.


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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23835250 - 11/15/16 11:48 AM (7 years, 2 months ago)

That plate looks clean man .. and what do you mean 10 transfers? You can 100000000 transfers to new agar if you mean 10 qts of grains then make a jar with like 100ml water and make your li and just equally disperse it ... an ms plate can get better with transfers 100% but you could end up with shit genetics like my orissa indias i grew


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23835262 - 11/15/16 11:53 AM (7 years, 2 months ago)

I was just wondering at what point you stop transferring, just "when it's clean"?

100ml of water for my LI for ten jars. Wasn't specifically asking that, however, I now have that noted as that's my gameplan.


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23835270 - 11/15/16 11:55 AM (7 years, 2 months ago)

You could make 250 just to be safe just dont pour too much ... and you have to keep transferring so its fresh and never dies off so its a cinstant job you could be on transfer 100 and get nasties in there so you get it


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23835273 - 11/15/16 11:56 AM (7 years, 2 months ago)

Ahhhhh. Now THERES some information.

I can keep this GT going via continuously transferring. Never have to get spores out again. Damn!

Big things coming friends. Can't wait.


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KSSS And PE WBS.
 


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23835275 - 11/15/16 11:58 AM (7 years, 2 months ago)

Well you may have to but if youre clean you shouldnt have to unless you want a different cube


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23835281 - 11/15/16 12:01 PM (7 years, 2 months ago)

Can I just store the plate in the fridge for an extended period of time?


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KSSS And PE WBS.
 


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23835291 - 11/15/16 12:03 PM (7 years, 2 months ago)

Ya thats what slants are for you could keep a plate im sure for a month or two ive never done it so im not sure...


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InvisibleJosex
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23835303 - 11/15/16 12:06 PM (7 years, 2 months ago)

Moab, when you play with agar you can choose lots of ways to do things. This is what I'll do as soon as I have my new apartment set up; I'm going to start with two varieties (mexican and AA+). I'm gonna noc 10 plates from prints for each variety (20 in total). Transfer leading edge from each plate to new plates, and I'm going to be repeating this until I see nice and organized growth (maybe 3 or 4 transfers).

Once I have nice growth in the 20 plates I'm gonna go ahead and and choose a number of them to noc some grains, BUT before that I'll transfer from those plates to new plates to keep isolating further, while at the same time I can have some "limited MS" grows going.

My goal is to keep isolating until I have 20 pure cultures to test out and in the meantime I'll be using donor plates to keep some monotubs going. I'd just rather work with isolates than clones, but that's just me.


Edited by Josex (11/15/16 12:52 PM)


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Josex]
    #23835318 - 11/15/16 12:12 PM (7 years, 2 months ago)

20 plates is alot for a beginner nless you only do one transfer on each i usually do 3 per plate ... just dont get overwhelmed moab keep it simple at first till youre comfy eith yourself


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23835333 - 11/15/16 12:16 PM (7 years, 2 months ago)

I have over 20 plates going, and as a beginner, it's fucking me up. Too much stuff going on. Gonna trash a few tomorrow.


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KSSS And PE WBS.
 


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23835341 - 11/15/16 12:19 PM (7 years, 2 months ago)

Let some pin for clones may take a minth but they should pin


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InvisibleComebackKid
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23835352 - 11/15/16 12:23 PM (7 years, 2 months ago)

Sorry man no pics of clean agar right now. I recently started a new project. Made a transfer from my last clean plate and flushed all my old cultures and plates lastnight.

Only advice I can give is that if there's myc and no other funny business then your good to go!


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleJosex
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23835354 - 11/15/16 12:23 PM (7 years, 2 months ago)

Quote:

noob47 said:
20 plates is alot for a beginner nless you only do one transfer on each i usually do 3 per plate ... just dont get overwhelmed moab keep it simple at first till youre comfy eith yourself




I think I'm going to do one transfer on each. I know Moab has set the bar high for himself :hehehe: The worst that could happen if he started 20 plates from MS is that he'd have a lot of cultures to work with and an awful lot of spawn


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Josex]
    #23835365 - 11/15/16 12:25 PM (7 years, 2 months ago)

That Asian store I got the agar from, had live fucking crabs, all sorts of live fishes, everything man.

They had shrimps wth heads on 5.99 a lb. got a pound of them, and just nuked one in the microwave.....


Best fucking shrimp I've ever ate that wasn't on the gulf coast. Holy SHIT


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23835372 - 11/15/16 12:29 PM (7 years, 2 months ago)

Also totally forgot that I got a Texas yellow Cap print in the mail yesterday that I'm real excited about. And a gently used PE6 print on the way....

Fuck me I love this hobby.


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InvisibleComebackKid
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Re: Cultivation General Discussion [Re: Josex]
    #23835375 - 11/15/16 12:30 PM (7 years, 2 months ago)

Quote:

Josex said:
Quote:

noob47 said:
20 plates is alot for a beginner nless you only do one transfer on each i usually do 3 per plate ... just dont get overwhelmed moab keep it simple at first till youre comfy eith yourself




I think I'm going to do one transfer on each. I know Moab has set the bar high for himself :hehehe: The worst that could happen if he started 20 plates from MS is that he'd have a lot of cultures to work with and an awful lot of spawn




Hey I started 12 plates last night. Can never have too many when trying to germ stubborn spores



--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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