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LizardWizard
GnomeGrower




Registered: 01/07/15
Posts: 13,692
Loc: the parking lot
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25136092 - 04/13/18 03:19 PM (5 years, 9 months ago) |
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In cultures like that, only time will tell IME. Tomentose growth occurs most often right after germination, but, in cubensis, will typically grow at least a little more rhizomorph over time, or when put on different media/substrates. I think in most other species of higher fungi as well. IME, cordyceps doesn't form rhizomorphs on agar, but that makes sense to me, as it's never on agar for long, since it ages too fast, which makes it a liability. You go straight to grains once you have expanded enough. But then in the grains, you'll eventually see it take on more rhizomorph characteristics. Whereas the "ice cream" look in grains points at a contam, just like the other extreme of ropey bacterial growth.
And even in that last pic mushboy, you can see that it IS more rhizomorphicly formed than that first pic!
-------------------- The best things in life can be smelled on one's fingers.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
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Re: Cultivation General Discussion [Re: LizardWizard]
#25136147 - 04/13/18 03:39 PM (5 years, 9 months ago) |
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Time doesn't seem to do shit. Could be the malt. I'll have to go back to pda, maybe it's time to try brf
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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LizardWizard
GnomeGrower




Registered: 01/07/15
Posts: 13,692
Loc: the parking lot
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25136150 - 04/13/18 03:40 PM (5 years, 9 months ago) |
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Different media give different behavior. Do give it at least 3 plates to run across to show the different behavior. Takes a while sometimes.
-------------------- The best things in life can be smelled on one's fingers.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25136151 - 04/13/18 03:40 PM (5 years, 9 months ago) |
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honestly? i prefer brf over potato d.
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Lemgrub


Registered: 04/02/18
Posts: 589
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Re: Cultivation General Discussion [Re: mushboy]
#25136170 - 04/13/18 03:48 PM (5 years, 9 months ago) |
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I only use Sabouraud's Dextrose Agar, which has a really high sugar content - 40g/L. Growth is pretty slow and in between rhizo and tomentose.
-------------------- We getting that pirate bay, alien shishkebab cordycep money

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Kyshroomer19xx
Est 19xx soldier



Registered: 04/22/17
Posts: 1,308
Loc:
Last seen: 4 years, 6 months
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Re: Cultivation General Discussion [Re: Lemgrub]
#25136190 - 04/13/18 03:54 PM (5 years, 9 months ago) |
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How long do you guys let your tubs sit before fruiting after spawning? I have had mine sitting for 7 days i have been waiting 10 cause thats what i read in the tek i first used cant remember what one right now.
-------------------- RIP tom petty Of course it is happening inside your head, but why on earth should that mean it is not real? Albus Dumbledore
Edited by Kyshroomer19xx (04/13/18 03:58 PM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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<--fruits at spawning
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Kyshroomer19xx
Est 19xx soldier



Registered: 04/22/17
Posts: 1,308
Loc:
Last seen: 4 years, 6 months
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Re: Cultivation General Discussion [Re: mushboy]
#25136202 - 04/13/18 03:59 PM (5 years, 9 months ago) |
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Ok cool i am going to fruit it
-------------------- RIP tom petty Of course it is happening inside your head, but why on earth should that mean it is not real? Albus Dumbledore
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Lemgrub


Registered: 04/02/18
Posts: 589
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I'm writing up a review of some literature on spore germination and found a nice little nugget.
Spore germination increased to 23% over a 7 day span in C. radiatus when exposed to a 45'C heat shock for four hours. This rate increased to 88% when heat shocked and exposed to chemicals such as furfural, commonly found in oats and other grains.
The evolutionary basis is interesting to think about to me. Radiatus is also a coprophile, so it likely passes through a cow or horse's stomach where it encounters those same 2 conditions. I think this strategy likely applies to cubensis as well.
source: http://mic.microbiologyresearch.org/content/journal/micro/10.1099/00221287-77-2-393
-------------------- We getting that pirate bay, alien shishkebab cordycep money

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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Lemgrub]
#25136465 - 04/13/18 06:04 PM (5 years, 9 months ago) |
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Quote:
Lemgrub said: I'm writing up a review of some literature on spore germination and found a nice little nugget.
Spore germination increased to 23% over a 7 day span in C. radiatus when exposed to a 45'C heat shock for four hours. This rate increased to 88% when heat shocked and exposed to chemicals such as furfural, commonly found in oats and other grains.
The evolutionary basis is interesting to think about to me. Radiatus is also a coprophile, so it likely passes through a cow or horse's stomach where it encounters those same 2 conditions. I think this strategy likely applies to cubensis as well.
source: http://mic.microbiologyresearch.org/content/journal/micro/10.1099/00221287-77-2-393
Every fungi is so different. There's probably a close to 0 chance that it will correlate to p.cubensis. might it, yes but the probability is way closer to 0% than it is to 100%
Oysters benefit from a cold shock for instance. Cubes absolutely do not.
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Lemgrub


Registered: 04/02/18
Posts: 589
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Re: Cultivation General Discussion [Re: bodhisatta]
#25136679 - 04/13/18 07:24 PM (5 years, 9 months ago) |
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Quote:
bodhisatta said:
Quote:
Lemgrub said: I'm writing up a review of some literature on spore germination and found a nice little nugget.
Spore germination increased to 23% over a 7 day span in C. radiatus when exposed to a 45'C heat shock for four hours. This rate increased to 88% when heat shocked and exposed to chemicals such as furfural, commonly found in oats and other grains.
The evolutionary basis is interesting to think about to me. Radiatus is also a coprophile, so it likely passes through a cow or horse's stomach where it encounters those same 2 conditions. I think this strategy likely applies to cubensis as well.
source: http://mic.microbiologyresearch.org/content/journal/micro/10.1099/00221287-77-2-393
Every fungi is so different. There's probably a close to 0 chance that it will correlate to p.cubensis. might it, yes but the probability is way closer to 0% than it is to 100%
Oysters benefit from a cold shock for instance. Cubes absolutely do not.
Very true. This studying I'm doing is more just to get back into the swing of research since I'm applying to some university research labs rn. The circumstantial evidence seems compelling to me in any case.
I'm rusty on genomics but I was thinking of doing some sequence alignments of related species for the shits.
The reproductive strategies can be an indicator however. Does pleurotus tend to grow in dung?
-------------------- We getting that pirate bay, alien shishkebab cordycep money

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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: Lemgrub]
#25136689 - 04/13/18 07:30 PM (5 years, 9 months ago) |
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It doesn't tend to in the wild, but I've grown it on a coir/dung substrate. https://www.shroomery.org/forums/showflat.php/Number/21482569#21482569
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LizardWizard
GnomeGrower




Registered: 01/07/15
Posts: 13,692
Loc: the parking lot
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Re: Cultivation General Discussion [Re: hamloaf]
#25137514 - 04/14/18 02:54 AM (5 years, 9 months ago) |
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lemgrub, the fuck is that sec pic in your sig? A dick-sucking table or something?
-------------------- The best things in life can be smelled on one's fingers.
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Leftfield420
bong toker



Registered: 02/26/16
Posts: 10,023
Last seen: 9 hours, 38 minutes
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Re: Cultivation General Discussion [Re: LizardWizard]
#25137702 - 04/14/18 06:43 AM (5 years, 9 months ago) |
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Looks like a fucked up version of a glory hole to me
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Kyshroomer19xx
Est 19xx soldier



Registered: 04/22/17
Posts: 1,308
Loc:
Last seen: 4 years, 6 months
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Re: Cultivation General Discussion [Re: Leftfield420]
#25137883 - 04/14/18 08:37 AM (5 years, 9 months ago) |
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Lol i just looked at that pic too and was wondering the same exact thing
-------------------- RIP tom petty Of course it is happening inside your head, but why on earth should that mean it is not real? Albus Dumbledore
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
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Can we have a conversation about lime vs heat pasturization? Can you only lime pasteurize straw? And is the main thing we are trying to do is lower the PH? If that's the case do I have to heat pasturize my peat considering I'll be adding lime? If so, why do I need both?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25138003 - 04/14/18 09:50 AM (5 years, 9 months ago) |
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Lime is alkaline and raises the ph. Ive never used it on straw but would guess people use it so they don’t have to heat and maintain pasteurization temps on such large volumes of water. If you’re referring to casings in the latter part of your question I would say the lime part of the equation is to render it less appetizing to the fungus you’re growing as well as to extend the efficacy of the heat pasteurization.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
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Re: Cultivation General Discussion [Re: Mycolorado]
#25138010 - 04/14/18 09:52 AM (5 years, 9 months ago) |
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Yea, I'm looking to whip up a casing. Thanks for the info!
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Lemgrub


Registered: 04/02/18
Posts: 589
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25138197 - 04/14/18 11:22 AM (5 years, 9 months ago) |
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Death grips - the year of the snitch cover art
-------------------- We getting that pirate bay, alien shishkebab cordycep money

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Kyshroomer19xx
Est 19xx soldier



Registered: 04/22/17
Posts: 1,308
Loc:
Last seen: 4 years, 6 months
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Re: Cultivation General Discussion [Re: Lemgrub]
#25138212 - 04/14/18 11:28 AM (5 years, 9 months ago) |
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I recently cut all the fruits from a mono and shoebox to try an get another flush. Some of the stumps turned into a nasty gooey looking stuff. Whats the best way to go about harvesting to get more flushes? I have twisted and pulled but it always tears up the sub bad
-------------------- RIP tom petty Of course it is happening inside your head, but why on earth should that mean it is not real? Albus Dumbledore
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