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InvisibleLizardWizard
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25136092 - 04/13/18 03:19 PM (5 years, 9 months ago)

In cultures like that, only time will tell IME. Tomentose growth occurs most often right after germination, but, in cubensis, will typically grow at least a little more rhizomorph over time, or when put on different media/substrates. I think in most other species of higher fungi as well. IME, cordyceps doesn't form rhizomorphs on agar, but that makes sense to me, as it's never on agar for long, since it ages too fast, which makes it a liability. You go straight to grains once you have expanded enough. But then in the grains, you'll eventually see it take on more rhizomorph characteristics. Whereas the "ice cream" look in grains points at a contam, just like the other extreme of ropey bacterial growth.

And even in that last pic mushboy, you can see that it IS more rhizomorphicly formed than that first pic!


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: LizardWizard]
    #25136147 - 04/13/18 03:39 PM (5 years, 9 months ago)

Time doesn't seem to do shit. Could be the malt. I'll have to go back to pda, maybe it's time to try brf


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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InvisibleLizardWizard
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25136150 - 04/13/18 03:40 PM (5 years, 9 months ago)

Different media give different behavior. Do give it at least 3 plates to run across to show the different behavior. Takes a while sometimes.


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25136151 - 04/13/18 03:40 PM (5 years, 9 months ago)

honestly? i prefer brf over potato d.


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InvisibleLemgrub
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Re: Cultivation General Discussion [Re: mushboy]
    #25136170 - 04/13/18 03:48 PM (5 years, 9 months ago)

I only use Sabouraud's Dextrose Agar, which has a really high sugar content - 40g/L. Growth is pretty slow and in between rhizo and tomentose.


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OfflineKyshroomer19xx
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Re: Cultivation General Discussion [Re: Lemgrub]
    #25136190 - 04/13/18 03:54 PM (5 years, 9 months ago)

How long do you guys let your tubs sit before fruiting after spawning? I have had mine sitting for 7 days i have been waiting 10 cause thats what i read in the tek i first used cant remember what one right now.


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RIP tom petty

Of course it is happening inside your head, but why on earth should that mean it is not real?
Albus  Dumbledore


Edited by Kyshroomer19xx (04/13/18 03:58 PM)


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Kyshroomer19xx]
    #25136200 - 04/13/18 03:56 PM (5 years, 9 months ago)

<--fruits at spawning


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OfflineKyshroomer19xx
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Re: Cultivation General Discussion [Re: mushboy]
    #25136202 - 04/13/18 03:59 PM (5 years, 9 months ago)

Ok cool i am going to fruit it


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RIP tom petty

Of course it is happening inside your head, but why on earth should that mean it is not real?
Albus  Dumbledore


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InvisibleLemgrub
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Re: Cultivation General Discussion [Re: Kyshroomer19xx] * 1
    #25136418 - 04/13/18 05:44 PM (5 years, 9 months ago)

I'm writing up a review of some literature on spore germination and found a nice little nugget.

Spore germination increased to 23% over a 7 day span in C. radiatus when exposed to a 45'C heat shock for four hours. This rate increased to 88% when heat shocked and exposed to chemicals such as furfural, commonly found in oats and other grains.

The evolutionary basis is interesting to think about to me. Radiatus is also a coprophile, so it likely passes through a cow or horse's stomach where it encounters those same 2 conditions. I think this strategy likely applies to cubensis as well.

source: http://mic.microbiologyresearch.org/content/journal/micro/10.1099/00221287-77-2-393


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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: Lemgrub]
    #25136465 - 04/13/18 06:04 PM (5 years, 9 months ago)

Quote:

Lemgrub said:
I'm writing up a review of some literature on spore germination and found a nice little nugget.

Spore germination increased to 23% over a 7 day span in C. radiatus when exposed to a 45'C heat shock for four hours. This rate increased to 88% when heat shocked and exposed to chemicals such as furfural, commonly found in oats and other grains.

The evolutionary basis is interesting to think about to me. Radiatus is also a coprophile, so it likely passes through a cow or horse's stomach where it encounters those same 2 conditions. I think this strategy likely applies to cubensis as well.

source: http://mic.microbiologyresearch.org/content/journal/micro/10.1099/00221287-77-2-393



Every fungi is so different. There's probably a close to 0 chance that it will correlate to p.cubensis. might it, yes but the probability is way closer to 0% than it is to 100%

Oysters benefit from a cold shock for instance. Cubes absolutely do not.


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InvisibleLemgrub
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Re: Cultivation General Discussion [Re: bodhisatta]
    #25136679 - 04/13/18 07:24 PM (5 years, 9 months ago)

Quote:

bodhisatta said:
Quote:

Lemgrub said:
I'm writing up a review of some literature on spore germination and found a nice little nugget.

Spore germination increased to 23% over a 7 day span in C. radiatus when exposed to a 45'C heat shock for four hours. This rate increased to 88% when heat shocked and exposed to chemicals such as furfural, commonly found in oats and other grains.

The evolutionary basis is interesting to think about to me. Radiatus is also a coprophile, so it likely passes through a cow or horse's stomach where it encounters those same 2 conditions. I think this strategy likely applies to cubensis as well.

source: http://mic.microbiologyresearch.org/content/journal/micro/10.1099/00221287-77-2-393



Every fungi is so different. There's probably a close to 0 chance that it will correlate to p.cubensis. might it, yes but the probability is way closer to 0% than it is to 100%

Oysters benefit from a cold shock for instance. Cubes absolutely do not.




Very true. This studying I'm doing is more just to get back into the swing of research since I'm applying to some university research labs rn. The circumstantial evidence seems compelling to me in any case.

I'm rusty on genomics but I was thinking of doing some sequence alignments of related species for the shits.

The reproductive strategies can be an indicator however. Does pleurotus tend to grow in dung?


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Offlinehamloaf
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Re: Cultivation General Discussion [Re: Lemgrub]
    #25136689 - 04/13/18 07:30 PM (5 years, 9 months ago)

It doesn't tend to in the wild, but I've grown it on a coir/dung substrate.
https://www.shroomery.org/forums/showflat.php/Number/21482569#21482569


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InvisibleLizardWizard
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Re: Cultivation General Discussion [Re: hamloaf]
    #25137514 - 04/14/18 02:54 AM (5 years, 9 months ago)

lemgrub, the fuck is that sec pic in your sig? A dick-sucking table or something?


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OfflineLeftfield420
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Re: Cultivation General Discussion [Re: LizardWizard]
    #25137702 - 04/14/18 06:43 AM (5 years, 9 months ago)

Looks like a fucked up version of a glory hole to me


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OfflineKyshroomer19xx
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Re: Cultivation General Discussion [Re: Leftfield420]
    #25137883 - 04/14/18 08:37 AM (5 years, 9 months ago)

Lol i just looked at that pic too and was wondering the same exact thing


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RIP tom petty

Of course it is happening inside your head, but why on earth should that mean it is not real?
Albus  Dumbledore


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Kyshroomer19xx]
    #25137977 - 04/14/18 09:36 AM (5 years, 9 months ago)

Can we have a conversation about lime vs heat pasturization?
Can you only lime pasteurize straw? And is the main thing we are trying to do is lower the PH? If that's the case do I have to heat pasturize my peat considering I'll be adding lime? If so, why do I need both?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleMycolorado
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25138003 - 04/14/18 09:50 AM (5 years, 9 months ago)

Lime is alkaline and raises the ph.  Ive never used it on straw but would guess people use it so they don’t have to heat and maintain pasteurization temps on such large volumes of water.  If you’re referring to casings in the latter part of your question I would say the lime part of the equation is to render it less appetizing to the fungus you’re growing as well as to extend the efficacy of the heat pasteurization.


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Mycolorado]
    #25138010 - 04/14/18 09:52 AM (5 years, 9 months ago)

Yea, I'm looking to whip up a casing. Thanks for the info!


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleLemgrub
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25138197 - 04/14/18 11:22 AM (5 years, 9 months ago)

Death grips - the year of the snitch cover art


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OfflineKyshroomer19xx
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Re: Cultivation General Discussion [Re: Lemgrub]
    #25138212 - 04/14/18 11:28 AM (5 years, 9 months ago)

I recently cut all the fruits from a mono and shoebox to try an get another flush. Some of the stumps turned into a nasty gooey looking stuff. Whats the best way to go about harvesting to get more flushes? I have twisted and pulled but it always tears up the sub bad


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RIP tom petty

Of course it is happening inside your head, but why on earth should that mean it is not real?
Albus  Dumbledore


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