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FishLevelMidnight
Aquaman



Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 25 days
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Re: Cultivation General Discussion [Re: LizardWizard]
#25135687 - 04/13/18 12:38 PM (5 years, 9 months ago) |
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Quote:
LizardWizard said: It's not the point to make them less visible, the point is to put a culture with hitchhiking contams onto a plate made with colloidal silver, as to give the culture the equivalent of an IV drip of antibiotics, but then in a more natural way since antibiotics are also known to weaken fungi. Run a contam culture over that media for 4-5 plates, and who knows, might do a whole lot better..? That's for him to find out.
Another why is for scientific advancement.
Which antibiotics weaken fungi, and which fungi? Isn’t that the whole point of antibiotics is that they are acting on cellular components that only prokaryotes have... fungi share the same cellular machinery as humans so for the most part, an antibiotic that also effects fungi will effect human cells...
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 12 hours, 46 seconds
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25135698 - 04/13/18 12:41 PM (5 years, 9 months ago) |
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Quote:
tryptkaloids said:
Quote:
tryptkaloids said: I'm still not fully sure if my cultures are bacterial or just puffy.. Is a raised culture a sign of bacteria or just morphology? It could be grain prep that's fuckin with me...
I can't be the only person to experience this... Nobody?
I've had puffy growth on clean plates, I think...
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
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Quote:
fishermansjc said:
Quote:
LizardWizard said: It's not the point to make them less visible, the point is to put a culture with hitchhiking contams onto a plate made with colloidal silver, as to give the culture the equivalent of an IV drip of antibiotics, but then in a more natural way since antibiotics are also known to weaken fungi. Run a contam culture over that media for 4-5 plates, and who knows, might do a whole lot better..? That's for him to find out.
Another why is for scientific advancement.
Which antibiotics weaken fungi, and which fungi? Isn’t that the whole point of antibiotics is that they are acting on cellular components that only prokaryotes have... fungi share the same cellular machinery as humans so for the most part, an antibiotic that also effects fungi will effect human cells...
don't antibiotics come from fungal enzymes?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Steevo
Just a cog in the death wheel



Registered: 03/02/17
Posts: 2,702
Loc: Here and There
Last seen: 1 year, 9 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25135732 - 04/13/18 12:56 PM (5 years, 9 months ago) |
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Are you talking about tomentose trypt? Any pics?
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: Steevo]
#25135772 - 04/13/18 01:12 PM (5 years, 9 months ago) |
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Not just tomentose, it's aerial. If you can call it that... If grows up a bit. I'll get a pic after work
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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LizardWizard
GnomeGrower




Registered: 01/07/15
Posts: 13,692
Loc: the parking lot
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Re: Cultivation General Discussion [Re: tryptkaloids] 1
#25135777 - 04/13/18 01:14 PM (5 years, 9 months ago) |
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Quote:
tryptkaloids said:
Quote:
fishermansjc said:
Quote:
LizardWizard said: It's not the point to make them less visible, the point is to put a culture with hitchhiking contams onto a plate made with colloidal silver, as to give the culture the equivalent of an IV drip of antibiotics, but then in a more natural way since antibiotics are also known to weaken fungi. Run a contam culture over that media for 4-5 plates, and who knows, might do a whole lot better..? That's for him to find out.
Another why is for scientific advancement.
Which antibiotics weaken fungi, and which fungi? Isn’t that the whole point of antibiotics is that they are acting on cellular components that only prokaryotes have... fungi share the same cellular machinery as humans so for the most part, an antibiotic that also effects fungi will effect human cells...
don't antibiotics come from fungal enzymes?
Got my shit mixed up, sorry.
-------------------- The best things in life can be smelled on one's fingers.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25135780 - 04/13/18 01:16 PM (5 years, 9 months ago) |
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you talkin about dis kinda thang?

where its more like a puff ball than a growing culture? opposite of these?
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
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Re: Cultivation General Discussion [Re: mushboy]
#25135847 - 04/13/18 01:40 PM (5 years, 9 months ago) |
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Yes
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25135849 - 04/13/18 01:41 PM (5 years, 9 months ago) |
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Forever I thought it was a sign of something hidden in there. But lately I'm leaving towards it being my grain prep
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Jeebo
Stranger
Registered: 06/06/17
Posts: 71
Last seen: 5 years, 7 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25135870 - 04/13/18 01:47 PM (5 years, 9 months ago) |
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I poured quite a few pasty plates now and I've literally never seen Rhizo growth, no idea why.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
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Re: Cultivation General Discussion [Re: Jeebo]
#25135895 - 04/13/18 01:56 PM (5 years, 9 months ago) |
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Try lowering the nutes
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Jeebo
Stranger
Registered: 06/06/17
Posts: 71
Last seen: 5 years, 7 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25135959 - 04/13/18 02:22 PM (5 years, 9 months ago) |
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currently at 5g to 500ml of agar, I think I already lowered them from the "standard" ratios a few times. not sure though.
My cultures had a lot of mold and bacteria running through them when I was working in a tiny sab, at what point would transferring away be pointless and be worth starting from spores again.
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Mr.Kaption
I breed snails


Registered: 02/16/18
Posts: 602
Last seen: 3 years, 6 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25135975 - 04/13/18 02:27 PM (5 years, 9 months ago) |
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Took me 9 transfers to start seeing rhizo with PE. I’m still using 10g ELME/ 10g agar.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: Jeebo]
#25135977 - 04/13/18 02:30 PM (5 years, 9 months ago) |
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Quote:
Jeebo said: currently at 5g to 500ml of agar, I think I already lowered them from the "standard" ratios a few times. not sure though.
My cultures had a lot of mold and bacteria running through them when I was working in a tiny sab, at what point would transferring away be pointless and be worth starting from spores again.
Agar is not nutrient source. Malt extract, potato broth/flakes, dog food etc are nutrient sources. Amount of agar used determines texture of agar (hard or soft).
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Jeebo
Stranger
Registered: 06/06/17
Posts: 71
Last seen: 5 years, 7 months
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Re: Cultivation General Discussion [Re: hamloaf]
#25136054 - 04/13/18 02:59 PM (5 years, 9 months ago) |
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Yeah I know that loafy 5g of LME Is what I was trying to say.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
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Re: Cultivation General Discussion [Re: mushboy]
#25136061 - 04/13/18 03:05 PM (5 years, 9 months ago) |
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Quote:
mushboy said: you talkin about dis kinda thang?

where its more like a puff ball than a growing culture? opposite of these?

Can nobody answer this question?.... Am I just seeing tomentose traits or is something hiding in there?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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sangreen
Dunce


Registered: 03/31/18
Posts: 182
Loc: Enceladus
Last seen: 1 year, 1 month
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25136072 - 04/13/18 03:09 PM (5 years, 9 months ago) |
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I prepared half my (tater-honey) agar media with 10ml less water than normal. I diluted 10ml of 10ppm colloidal silver in 240ml of media, resulting in a solution that is 400ppb colloidal silver. The other half of the media was prepared with no special additives. Bottles were PCed at 17 PSI for 40 minutes (I'm compensating for high altitude :P) and poured in an SAB using a standard technique. I got 10 plates of the regular stuff (which was poured first) and 11 plates of the silver stuff (probably due to refinement of technique). I took a regular dish and a silver dish (once cooled) and swabbed them with a cheek scraping- 12 hours later, nothing on either, but I'll keep people updated on my idiot experiment. There wasn't a difference in consistency between the regular and silver stuff, either liquid or solid.
-------------------- I figured it out, what life is about It crawled into my brain and it's starting to sprout
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25136082 - 04/13/18 03:13 PM (5 years, 9 months ago) |
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Quote:
tryptkaloids said:
Quote:
mushboy said: you talkin about dis kinda thang?

where its more like a puff ball than a growing culture? opposite of these?

Can nobody answer this question?.... Am I just seeing tomentose traits or is something hiding in there?
one is brf one is light malt(blue). the brf is also less nutes, 1%. so heed your own advice and use less nutes.. or change up the media.
or dont do anything because .....?
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: mushboy]
#25136086 - 04/13/18 03:15 PM (5 years, 9 months ago) |
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They're different cultures though... Did you take that first one to grain?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: mushboy]
#25136089 - 04/13/18 03:17 PM (5 years, 9 months ago) |
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ackchuaallllyy,
the lgt i got on malt will be the first time i put it to 1% brf.. which im doing later. on malt its aalways fuzzy
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