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InvisiblemushboyMDiscord
modboy
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Registered: 04/24/05
Posts: 32,281
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OG Cultivator
Re: Cultivation General Discussion [Re: bodhisatta]
    #25114031 - 04/04/18 04:06 PM (5 years, 9 months ago)

it LOOKS waxy.. not it IS wax:takingnotes:


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InvisibleJHOVA
Post whore
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Registered: 02/17/17
Posts: 4,727
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Re: Cultivation General Discussion [Re: mushboy]
    #25114036 - 04/04/18 04:08 PM (5 years, 9 months ago)

Yea its like when you take inventory for belongings “silver color watch” is not the same as a silver watch.


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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InvisibleLemgrub
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Registered: 04/02/18
Posts: 589
Re: Cultivation General Discussion [Re: JHOVA]
    #25114273 - 04/04/18 06:14 PM (5 years, 9 months ago)

I'm top fruiting a jar I thought was bacterial, and there's a significant amount of water pooling towards the bottom. Is this likely to get incorporated in the fruits or should I attempt to pour out? It's probably around 20-30ml of water.


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We getting that pirate bay, alien shishkebab cordycep money


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Offlinespace_shrooms


Registered: 05/04/17
Posts: 438
Last seen: 5 months, 1 day
Re: Cultivation General Discussion [Re: Lemgrub]
    #25114377 - 04/04/18 07:00 PM (5 years, 9 months ago)

Anyone else noticed pan cyans eat food coloring? I made LCs with bright blue agar from a cube plate, an oyster plate and a pan plate. All three turned blue as the liquid took on the color from the agar, but a few weeks later now they're colonised and the cube and oyster remain blue while the pan cyan has turned crystal clear. :shrug:


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InvisibleGerms
Space Force
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Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: space_shrooms] * 1
    #25114392 - 04/04/18 07:05 PM (5 years, 9 months ago)

Quote:

aicaca said:
Anyone else noticed pan cyans eat food coloring? I made LCs with bright blue agar from a cube plate, an oyster plate and a pan plate. All three turned blue as the liquid took on the color from the agar, but a few weeks later now they're colonised and the cube and oyster remain blue while the pan cyan has turned crystal clear. :shrug:



I haven’t used food coloring with any species but I have never heard of that, very cool indeed. Any pics?


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: space_shrooms] * 1
    #25114420 - 04/04/18 07:12 PM (5 years, 9 months ago)

Quote:

aicaca said:
Anyone else noticed pan cyans eat food coloring? I made LCs with bright blue agar from a cube plate, an oyster plate and a pan plate. All three turned blue as the liquid took on the color from the agar, but a few weeks later now they're colonised and the cube and oyster remain blue while the pan cyan has turned crystal clear. :shrug:



Ive noticed every mycelium eats food coloring. Food coloring is cool for keeping recipes separate but sucks for helping see what's going on on a dish


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Offlinespace_shrooms


Registered: 05/04/17
Posts: 438
Last seen: 5 months, 1 day
Re: Cultivation General Discussion [Re: Germs]
    #25114439 - 04/04/18 07:19 PM (5 years, 9 months ago)

Yea, the novelty has worn off.

Quote:

Germs said:
I haven’t used food coloring with any species but I have never heard of that, very cool indeed. Any pics?






1. pan cyan 2. oyster 3. cube

All of them from the same batch of blue plates, into the LC about 3 weeks ago.


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: Lemgrub]
    #25114517 - 04/04/18 07:59 PM (5 years, 9 months ago)

Quote:

Lemgrub said:
I'm top fruiting a jar I thought was bacterial, and there's a significant amount of water pooling towards the bottom. Is this likely to get incorporated in the fruits or should I attempt to pour out? It's probably around 20-30ml of water.



How sure are you it's water and not metabolites or bacterial soup? How long has it been there?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleLemgrub
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Registered: 04/02/18
Posts: 589
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25114855 - 04/05/18 12:05 AM (5 years, 9 months ago)

It's free of any off colors and while I'm sure there's some bacteria in the jar it's less than what I thought. I'm still trying to get my prep right, so these rye berries were loaded a bit too wet. The top just started forming primordia.


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We getting that pirate bay, alien shishkebab cordycep money


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OfflineGhostveil25
Electron


Registered: 02/04/18
Posts: 372
Loc: Oregon Flag
Last seen: 2 years, 1 month
Re: Cultivation General Discussion [Re: Lemgrub]
    #25114961 - 04/05/18 02:01 AM (5 years, 9 months ago)

Ive been reading up a lot on the possibility of cloning a dried specimen. I took a big dried mushroom and tore it open in the SAB. Everything was done in a sterile environment. I put gill material in a few dishes and a couple scrappings from the inner most part of the stem.

Its been two days since they were put to agar and there has been no activity as far as contamination. Im really hoping it works, but the more i read the more i realize it may be a very difficult process.

Also, i made my first spore syringe and put it to agar. We'll see what happens. Just wanted to share that with yall.

Thanks, Ghost


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InvisibleLemgrub
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Registered: 04/02/18
Posts: 589
Re: Cultivation General Discussion [Re: Ghostveil25]
    #25115070 - 04/05/18 04:38 AM (5 years, 9 months ago)

It took a minute for me, about 2 weeks before I could transfer. I hydrated in tap water for about 10 sec then dunked in iso, then plated. I put 3 samples on one plate to increase chance of recovery. The growth looked terrible, but after I cleaned it up and allowed it to fruit, the growth from the first clone looks very nice.


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We getting that pirate bay, alien shishkebab cordycep money


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OfflineCrispykoot
Jello Wrangler
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Registered: 10/16/16
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Re: Cultivation General Discussion [Re: bodhisatta]
    #25115126 - 04/05/18 05:50 AM (5 years, 9 months ago)

Quote:

bodhisatta said:
Quote:

aicaca said:
Anyone else noticed pan cyans eat food coloring? I made LCs with bright blue agar from a cube plate, an oyster plate and a pan plate. All three turned blue as the liquid took on the color from the agar, but a few weeks later now they're colonised and the cube and oyster remain blue while the pan cyan has turned crystal clear. :shrug:



Ive noticed every mycelium eats food coloring. Food coloring is cool for keeping recipes separate but sucks for helping see what's going on on a dish




I think food coloring is really good for seeing contaminants on plates. I wouldn't use colored for a LC but the contrast seems to really help.


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:gd_icon: Shadowboxing the apocalypse and wandering the land :gd_icon:


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OfflineLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,023
Last seen: 6 hours, 30 minutes
Re: Cultivation General Discussion [Re: Crispykoot]
    #25115171 - 04/05/18 06:29 AM (5 years, 9 months ago)

I think I got a sporeless lgt fruit...gonna check on it later...and most likely will clone it... I think all of us want a tub of sporeless fruits...


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Registered: 04/30/13
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Re: Cultivation General Discussion [Re: Leftfield420]
    #25115201 - 04/05/18 06:46 AM (5 years, 9 months ago)

I find transparent agar to be best for seeing anything going on. But whatever works for you is the best for you


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 10 hours
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Re: Cultivation General Discussion [Re: Lemgrub]
    #25115319 - 04/05/18 07:52 AM (5 years, 9 months ago)

Quote:

Lemgrub said:
It's free of any off colors and while I'm sure there's some bacteria in the jar it's less than what I thought. I'm still trying to get my prep right, so these rye berries were loaded a bit too wet. The top just started forming primordia.



I never let water stagnate for more than 24 hrs


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 10 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25115374 - 04/05/18 08:31 AM (5 years, 9 months ago)

What's the cheapest source for large mycobags?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinehamloaf
Loaf of Fam.
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Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25115378 - 04/05/18 08:34 AM (5 years, 9 months ago)

"Unicorn" down in Plano, Tx has some great deals on durable spawn bags.


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How I Get Stuff done. - My Reference Guide. - My Grows.


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OfflineSteevo
Just a cog in the death wheel
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Registered: 03/02/17
Posts: 2,702
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Re: Cultivation General Discussion [Re: hamloaf]
    #25115398 - 04/05/18 08:43 AM (5 years, 9 months ago)

You have to buy 1000 at a time with those Unicorn bags though don’t you?

I don’t have $300 to drop on bags all at once so I tend to go 30-50 at a time


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OfflineFishLevelMidnight
Aquaman
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Registered: 09/01/17
Posts: 2,328
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Re: Cultivation General Discussion [Re: Steevo]
    #25115418 - 04/05/18 08:54 AM (5 years, 9 months ago)

Quote:

Steevo said:
You have to buy 1000 at a time with those Unicorn bags though don’t you?

I don’t have $300 to drop on bags all at once so I tend to go 30-50 at a time




I've been working through my 100 bags I ordered. Was $60 or $65 with shipping and they are sturdy. Not as bad as dropping $300 at once, but I still feel like I got a good deal at 0.65$ a bag. I only use them for sterilization of substrates (I don't do monos anymore so I never need more than a quart of grain) for edibles so they should last me a little while.


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OfflineLeftfield420
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Male


Registered: 02/26/16
Posts: 10,023
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Re: Cultivation General Discussion [Re: FishLevelMidnight]
    #25115708 - 04/05/18 11:38 AM (5 years, 9 months ago)



Fuckin huge oyster mushroom... It's a little old but I'm gonna try to clone it


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