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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25111273 - 04/03/18 03:17 PM (5 years, 9 months ago)

Rgs is rye grass seed its small and is completely different than rye.

RGS imo sucks. You can do 1 parts water to 2 parts seed by volume.
Load into jars and pressure cook.


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Invisiblenatedawgnow
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Re: Cultivation General Discussion [Re: bodhisatta] * 1
    #25111299 - 04/03/18 03:23 PM (5 years, 9 months ago)

@trypt-rye grass seed is definitely a thing bro. Used to work on a golf course
and they used winter rye grass seed every fall through spring and bermuda in the summer.

Winter rye is a grain used as a cover crop, winter ryegrass is a grass best grown in winter


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OfflineSteevo
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Re: Cultivation General Discussion [Re: bodhisatta]
    #25111328 - 04/03/18 03:29 PM (5 years, 9 months ago)

Sucks or not I’d like to see up to date comparisons for stone producers

Thanks bod

Trypt I have no idea what you’re talking about


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OfflineFailboat
Fuck Up

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Re: Cultivation General Discussion [Re: Steevo]
    #25111341 - 04/03/18 03:32 PM (5 years, 9 months ago)

I would only use it as a G2G master, but that said I will not buy it again. Id rather grab wbs.


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InvisibleLemgrub
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Registered: 04/02/18
Posts: 589
Re: Cultivation General Discussion [Re: Steevo]
    #25111351 - 04/03/18 03:33 PM (5 years, 9 months ago)

Here's an alrite prep for rgs I've used. I kinda like working with rgs, spreads myc very well after a shake and the seeds won't burst. Kinda smells like tea when PCing.

https://www.shroomery.org/forums/showflat.php/Number/19937837


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We getting that pirate bay, alien shishkebab cordycep money


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OfflineSteevo
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Re: Cultivation General Discussion [Re: Failboat]
    #25111373 - 04/03/18 03:40 PM (5 years, 9 months ago)

Has anyone rehydrated RGS before PC'ing?

I'm interested in making up batches of hydrated grain mixed in with other sub ingredients for side by side comparisons


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OfflineFailboat
Fuck Up

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Re: Cultivation General Discussion [Re: Steevo]
    #25111399 - 04/03/18 03:47 PM (5 years, 9 months ago)

I have, its touchy. I used it on its own and mixed with millet and spring berries


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Invisibleboom boom

Registered: 02/27/18
Posts: 429
Re: Cultivation General Discussion [Re: Failboat]
    #25111918 - 04/03/18 07:34 PM (5 years, 9 months ago)

How does senescence work, exactly? When does it "reset?" For instance, germinated spores are obviously the furthest from senescence. But what about a clone tissue? If you grew out a monotub from a clone culture, then took out a clone from the resulting harvest and made a monotub from that culture, and did that over and over, you'd begin to have issues with senescence at some point, yes? I'm guessing cloning is similar to making a transfer on agar in that regard. The more you do it, the older the culture gets, and the growth will eventually start to stagnate.

On a similar note, does the amount of volume that a culture has colonized play a role? Say we have a clean petri with newly germinated spores on it. For sample A, we take a transfer from this petri and inoculate a BRF cake. For sample B, we inoculate a few jars, G2G those jars once or twice, then spawn to bulk in a monotub. If we take a clone from the BRF cake in sample A, and a clone from the monotub in sample B, is the BRF clone going to be fresher and perhaps grow more aggressively since it has colonized a lot less substrate since it was germinated?


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InvisibleLemurLemur
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Registered: 01/30/17 Happy 7th Shroomiversary!
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Re: Cultivation General Discussion [Re: bodhisatta]
    #25112114 - 04/03/18 08:46 PM (5 years, 9 months ago)

The manual for my presto 23qt says i cant use it on stoces that use flames or it'll cause warping.

Is there a way to use a gas top stove without damaging my pc? Like if i use a real low flame?


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(when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]


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Invisiblethelanzii

Registered: 11/13/12
Posts: 5,434
Re: Cultivation General Discussion [Re: LemurLemur]
    #25112118 - 04/03/18 08:47 PM (5 years, 9 months ago)

i have used gas flames on my 16qt 90% of its usage and its not warped

jsut go easy on it


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: natedawgnow]
    #25112121 - 04/03/18 08:48 PM (5 years, 9 months ago)

Quote:

bodhisatta said:
Rgs is rye grass seed its small and is completely different than rye.

RGS imo sucks. You can do 1 parts water to 2 parts seed by volume.
Load into jars and pressure cook.



Quote:

natedawgnow said:
@trypt-rye grass seed is definitely a thing bro. Used to work on a golf course
and they used winter rye grass seed every fall through spring and bermuda in the summer.

Winter rye is a grain used as a cover crop, winter ryegrass is a grass best grown in winter



Why the fuck do we bring the word rye into it? Fuckin pisses me off. Let's just call it what it is, grass seed. It's all the same. We want grain anyway. Only reason I use grass seed is to make up for poor straining.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Invisiblethelanzii

Registered: 11/13/12
Posts: 5,434
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25112124 - 04/03/18 08:48 PM (5 years, 9 months ago)

fuck rye grass seed

its so easy to fuck up the prep


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: LemurLemur]
    #25112128 - 04/03/18 08:49 PM (5 years, 9 months ago)

Quote:

LemurLemur said:
The manual for my presto 23qt says i cant use it on stoces that use flames or it'll cause warping.

Is there a way to use a gas top stove without damaging my pc? Like if i use a real low flame?



Sorry for the double post, look at the btu's in your stove and the manual of your pc. I think the limit is 6000?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinehamloaf
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Registered: 12/23/09
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Re: Cultivation General Discussion [Re: boom boom]
    #25112134 - 04/03/18 08:53 PM (5 years, 9 months ago)

Quote:

boom boom said:
How does senescence work, exactly? When does it "reset?" For instance, germinated spores are obviously the furthest from senescence. But what about a clone tissue? If you grew out a monotub from a clone culture, then took out a clone from the resulting harvest and made a monotub from that culture, and did that over and over, you'd begin to have issues with senescence at some point, yes? I'm guessing cloning is similar to making a transfer on agar in that regard. The more you do it, the older the culture gets, and the growth will eventually start to stagnate.

On a similar note, does the amount of volume that a culture has colonized play a role? Say we have a clean petri with newly germinated spores on it. For sample A, we take a transfer from this petri and inoculate a BRF cake. For sample B, we inoculate a few jars, G2G those jars once or twice, then spawn to bulk in a monotub. If we take a clone from the BRF cake in sample A, and a clone from the monotub in sample B, is the BRF clone going to be fresher and perhaps grow more aggressively since it has colonized a lot less substrate since it was germinated?



Have a look through this thread.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisibleLemgrub
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Registered: 04/02/18
Posts: 589
Re: Cultivation General Discussion [Re: boom boom] * 2
    #25112143 - 04/03/18 08:56 PM (5 years, 9 months ago)

Quote:

boom boom said:
How does senescence work, exactly? When does it "reset?" For instance, germinated spores are obviously the furthest from senescence. But what about a clone tissue? If you grew out a monotub from a clone culture, then took out a clone from the resulting harvest and made a monotub from that culture, and did that over and over, you'd begin to have issues with senescence at some point, yes? I'm guessing cloning is similar to making a transfer on agar in that regard. The more you do it, the older the culture gets, and the growth will eventually start to stagnate.

On a similar note, does the amount of volume that a culture has colonized play a role? Say we have a clean petri with newly germinated spores on it. For sample A, we take a transfer from this petri and inoculate a BRF cake. For sample B, we inoculate a few jars, G2G those jars once or twice, then spawn to bulk in a monotub. If we take a clone from the BRF cake in sample A, and a clone from the monotub in sample B, is the BRF clone going to be fresher and perhaps grow more aggressively since it has colonized a lot less substrate since it was germinated?





It comes down to telomeric degradation. As mitosis carries on through thousands of generations, each time DNA polymerase comes to the very end of the chromosome there's a hindrance that forces it to terminate the copy a few bases short of the original. That's why telomeres are non-coding, so that the loss in info doesn't matter until a significant number of generations. There's other reasons a culture may lose virility, some epigenetic.


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We getting that pirate bay, alien shishkebab cordycep money


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Invisiblevan hattonFacebookDiscord
Still a noob
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Registered: 11/23/14
Posts: 5,617
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25112160 - 04/03/18 09:03 PM (5 years, 9 months ago)

Quote:

tryptkaloids said:
Quote:

LemurLemur said:
The manual for my presto 23qt says i cant use it on stoces that use flames or it'll cause warping.

Is there a way to use a gas top stove without damaging my pc? Like if i use a real low flame?



Sorry for the double post, look at the btu's in your stove and the manual of your pc. I think the limit is 6000?




9000 btus is what it says in the manual.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Offlinetryptkaloids
Learner
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Registered: 02/08/15
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Re: Cultivation General Discussion [Re: Lemgrub] * 3
    #25112165 - 04/03/18 09:05 PM (5 years, 9 months ago)

Damn, what a Mic drop for your third post! Good to have you around!


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25112173 - 04/03/18 09:08 PM (5 years, 9 months ago)

How thick do I let my lc get before use?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinehamloaf
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Re: Cultivation General Discussion [Re: tryptkaloids] * 1
    #25112177 - 04/03/18 09:09 PM (5 years, 9 months ago)

It's up to you.  The thicker it gets the more inoculum you have.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisibleLemgrub
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Registered: 04/02/18
Posts: 589
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25112190 - 04/03/18 09:14 PM (5 years, 9 months ago)

Quote:

tryptkaloids said:
Damn, what a Mic drop for your third post! Good to have you around!




:wink:

I've been stalking this place for a while, finally started cultivating several months ago. The amount of info that goes into this hobby was somewhat staggering, but I'm getting the hang of it. Spores are pissing me off, the only culture I have to work with was from a dried fruit my friend gave me that I've reinvigorated. The epigenetic change it went through after I finally got it to fruit was remarkable.

Anyway I'm glad to finally be on here. I try to do some research on the L-Trp -> Psilocybin pathway in my spare time. The other night I think I found the rate limiting step in the ezymatic synthesis, but I doubt it has much practical utility. If I knew how to code I could actually do some productive genomics research.


--------------------
We getting that pirate bay, alien shishkebab cordycep money


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