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OfflineThedenthead
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Registered: 04/19/16
Posts: 531
Last seen: 4 months, 27 days
Re: Cultivation General Discussion [Re: Asura]
    #25104755 - 03/31/18 02:26 PM (5 years, 9 months ago)

Alright, I’m gonna let them go a few more days. Gotta prep more plates anyway. I was surprised that out of the 9 plates I put the spores too none have shown any bacteria. Some haven’t progressed at all but no obvious bacteria on them. Guess the print I had was relatively “clean”.


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Nothing but my meandering thoughts.

An ounce of prevention is worth a pound of cure.


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InvisibleJHOVA
Post whore
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Registered: 02/17/17
Posts: 4,727
Loc: Flag
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25104757 - 03/31/18 02:27 PM (5 years, 9 months ago)

Ginger root tea.


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
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Re: Cultivation General Discussion [Re: Thedenthead]
    #25104791 - 03/31/18 02:53 PM (5 years, 9 months ago)

Quote:

Thedenthead said:
Alright, I’m gonna let them go a few more days. Gotta prep more plates anyway. I was surprised that out of the 9 plates I put the spores too none have shown any bacteria. Some haven’t progressed at all but no obvious bacteria on them. Guess the print I had was relatively “clean”.



I would transfer now. You may have clean enough after first transfer, I had a clean germination plate I used the other day


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineCanharo
Glassblowing Extraordinaire
I'm a teapot


Registered: 10/27/14
Posts: 327
Last seen: 7 months, 29 days
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25105411 - 03/31/18 08:07 PM (5 years, 9 months ago)

Yeah I've been trying to work with agar but I feel like I'm not having alot of luck.  Will keep up though refuse to quit, but I'd love some feedback or advice anyone is willing to offer. 
This is a 2nd transfer away from MS after I already took the 3rd transfers a while back and its grown out a bit.  Since it was T2 I wanted to transfer the leading edge again to make sure its clean. Was unsure if its was rhizo on one side and cottony on the other because of genetics or if there was a contamination present on oneside or the other.


Heres what the 3rd Transfers look like after transfering the leading rhizomorphic edge


I've been using the advanced search engine like crazy & most I could find that looked like this transparent like mycelium? (mold?) was this from this response from bod 9 months ago
Quote:

bodhisatta said:



clear growth like this tightens up, if it's just the leading edge like that it's fine but your plates don't look like cubensis mycelium to me. 




Did I get unlucky and just transfer weak genetics that are thicking up? Am I riding bacteria, or is this mold?  All the post I find that sound like what I'm seeing have the photos have deleted. 

Also these how do they look?  Different culture but still cubes, am I being paranoid?  Or is my paranoia justifiable?


I'm reading on here as many hours a day as I can haha, I'm using MEA and the only difference between t2 & t3s was t3 had slightly less MEA (0.5G difference, Agar 5G to MEA 4.5G to 250ml water) sterilized for 45 minutes at 15 psi & work in a still air box.  Room has a hepa running hours befor work, shut down 3 hours befor SAB work, SAB has raised rack, damp soapy towl freshly laundered.  I bathe in 70% iso my arms and gloves, and spray a 10% bleach solution im the air an hour befor going in to work and my tools sit in alcohol till they're flamed outside and brought into the SAB as needed


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: Canharo]
    #25105570 - 03/31/18 09:29 PM (5 years, 9 months ago)

It's all looking good. First pic is just genetics. Second pics appear to be disliking the mutes provided. I would switch to something else. Like brf or potato flakes. You could probably even fix it by changing the amount of mea you use.  Third set of pics looks great


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineAtomHeart
MK Ultra Operative
Male


Registered: 05/05/06
Posts: 397
Last seen: 3 years, 1 month
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25107326 - 04/01/18 08:27 PM (5 years, 9 months ago)

Almost no better feeling in this world than spending 3 hours prepping and sterilizing some grain jars, just to accidentally pull the lid off of one in open air because it was unscrewed too far.


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You once ripped a man's jaw off...I seent it!



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OfflineMooseShroom
Wandering Soul


Registered: 05/24/17
Posts: 332
Last seen: 8 days, 6 hours
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25107352 - 04/01/18 08:42 PM (5 years, 9 months ago)

Quote:

tryptkaloids said:
It's all looking good. First pic is just genetics. Second pics appear to be disliking the mutes provided. I would switch to something else. Like brf or potato flakes. You could probably even fix it by changing the amount of mea you use.  Third set of pics looks great




How would you determine if you should add more nutes or remove some?


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: MooseShroom] * 1
    #25107408 - 04/01/18 09:06 PM (5 years, 9 months ago)

I wouldn't go under 1.3% or over 2.5%


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineMooseShroom
Wandering Soul


Registered: 05/24/17
Posts: 332
Last seen: 8 days, 6 hours
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25107433 - 04/01/18 09:19 PM (5 years, 9 months ago)

Quote:

tryptkaloids said:
I wouldn't go under 1.3% or over 2.5%




Just trial and error surrounding those numbers then? I've had some growth like that on my plates but it seemed to be more on the 1.5% plates than the 2% plates now that I think about it.


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: MooseShroom]
    #25107442 - 04/01/18 09:26 PM (5 years, 9 months ago)

Yep.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Invisiblethelanzii

Registered: 11/13/12
Posts: 5,434
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25107466 - 04/01/18 09:50 PM (5 years, 9 months ago)

what the go to wbs brand?


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InvisibleJHOVA
Post whore
Male User Gallery


Registered: 02/17/17
Posts: 4,727
Loc: Flag
Re: Cultivation General Discussion [Re: thelanzii]
    #25107486 - 04/01/18 10:03 PM (5 years, 9 months ago)

pennington classic is mostly milo and millet with a little sunflower mixed in. you can remove the sunflowers right before straining.


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InvisibleLemurLemur
Pray for Boog
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Registered: 01/30/17 Happy 7th Shroomiversary!
Posts: 6,004
Loc: Drinking on the roof Flag
Re: Cultivation General Discussion [Re: thelanzii]
    #25107557 - 04/01/18 11:03 PM (5 years, 9 months ago)

^The green label for red milo dominate, light blue label for more yellow millet.


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(when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]


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OfflineTheBlackCat
Possibly Human
Female User Gallery


Registered: 10/05/16
Posts: 2,819
Last seen: 1 year, 5 months
Re: Cultivation General Discussion [Re: LemurLemur]
    #25107599 - 04/01/18 11:42 PM (5 years, 9 months ago)

Sorry I haven't been around. I've been busy being herded around like cattle through apartments every day. But I'm still around.


I built a temporary Martha but my wild oysters are really unhappy in it. They look all shitty and leggy. Any recommendations for a good Martha timer and fan setup. Any links on here I can read up on it better. I'm just super busy and really want to fruit these bags I've hd  Any amazon links. Looking for something heap and two day. I just don't know how to search for the timers that come on for a few minutes every hour. I keep getting the regular one hour timers. What a good search term?


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Offlinehamloaf
Loaf of Fam.
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Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: TheBlackCat]
    #25107782 - 04/02/18 04:07 AM (5 years, 9 months ago)

Go to Amazon.  Use search words "Timer Outlet, Nearpow Multifunctional Infinite Cycle Programmable Plug-in Digital Timer Switch With 3-prong Outlet for Appliances, Energy-saving Timer, 15A/1800W"


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How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisibleMiracleMike
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Folding@home Statistics
Registered: 02/15/16
Posts: 176
Re: Cultivation General Discussion [Re: hamloaf]
    #25107793 - 04/02/18 04:23 AM (5 years, 9 months ago)

I think I caught flu. Is it dangerous for my shrooms? I got 2 trays with B+ and Golden Teacher and they just start pinnig 2 days ago. And Also I've got big mono with B+ in colonization process. I worry cause I grow em in the room I living and I have no place to move them. Is it possible to contaminate shrooms with my respiratory decease?


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Technically alcohol is a solution


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Invisiblethelanzii

Registered: 11/13/12
Posts: 5,434
Re: Cultivation General Discussion [Re: MiracleMike]
    #25107804 - 04/02/18 04:41 AM (5 years, 9 months ago)

as long as the spawn is clean you are fine

open air is far from sterile with or without your flu


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OfflineSteevo
Just a cog in the death wheel
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Registered: 03/02/17
Posts: 2,702
Loc: Here and There
Last seen: 1 year, 9 months
Re: Cultivation General Discussion [Re: MiracleMike]
    #25108033 - 04/02/18 08:33 AM (5 years, 9 months ago)

Quote:

MiracleMike said:
I think I caught flu. Is it dangerous for my shrooms? I got 2 trays with B+ and Golden Teacher and they just start pinnig 2 days ago. And Also I've got big mono with B+ in colonization process. I worry cause I grow em in the room I living and I have no place to move them. Is it possible to contaminate shrooms with my respiratory decease?




You’re just takin a piss yeah? :lol:


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: Steevo]
    #25108056 - 04/02/18 08:50 AM (5 years, 9 months ago)

Mushrooms are resistant to human pathogens


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleLizardWizard
GnomeGrower
I'm a teapot User Gallery


Folding@home Statistics
Registered: 01/07/15
Posts: 13,692
Loc: the parking lot
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25108170 - 04/02/18 10:14 AM (5 years, 9 months ago)

Mushrooms can be human pathogens.


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The best things in life
can be smelled on one's fingers.


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