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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25096727 - 03/28/18 09:57 AM (5 years, 9 months ago) |
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lme gives you that dope clear as glass look. i use cold water hydration.


water and lme into a jug and put in the fridge. shake every so often. pour into bottles and pc
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Cultivation General Discussion [Re: mushboy]
#25096736 - 03/28/18 10:00 AM (5 years, 9 months ago) |
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I use LME for plates as well as LC. Those look great mushboy! That Pan LC is thick!
Edited by Mycolorado (03/28/18 10:02 AM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Mycolorado]
#25096744 - 03/28/18 10:03 AM (5 years, 9 months ago) |
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thanks mang
i used it on some stuff im about to fruit.. super excited.
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Ghostveil25
Electron


Registered: 02/04/18
Posts: 372
Loc: Oregon
Last seen: 2 years, 1 month
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25096792 - 03/28/18 10:20 AM (5 years, 9 months ago) |
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My mixture is 10grams LME, 9 grams agar agar and 500ml water. How would i adjust that to make it good for spore germination. I think my mixture was even higher when i swiped the dishes with spores. Maybe 15 gr LME 10 gr agar 500ml water.
Id love to start with my own prints i just havent had success yet. At least the dishes didnt contaminate. That tells me my sterile tech was good, just my inoculation tech isnt so good.
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Tookitooki
Mycological Fabricator


Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Cultivation General Discussion [Re: mushboy]
#25096793 - 03/28/18 10:20 AM (5 years, 9 months ago) |
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I was referring to lme and Dex mix for lc. Seems most people got away from putting dextrose in things.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Ghostveil25]
#25096806 - 03/28/18 10:26 AM (5 years, 9 months ago) |
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you want a softer agar for stubborn spores so dont go up a gram
Quote:
Ghostveil25 said: just my inoculation tech isnt so good.
you get better every time you work(hopefully)
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: mushboy]
#25096903 - 03/28/18 10:53 AM (5 years, 9 months ago) |
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Tooki try the poke it's super easy and clean.
Shake the bag
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: Tookitooki]
#25097015 - 03/28/18 11:27 AM (5 years, 9 months ago) |
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Quote:
Tookitooki said: I was referring to lme and Dex mix for lc. Seems most people got away from putting dextrose in things.
It's not necessary. There's already plenty of sugar in malt. Addition of dextrose is overkill, and can send culture into diabetic shock.
On topic of sediment; I don't worry about it. If liquid media broth was PCed correctly it should be very translucent. Sediment settles at bottom. Myce consolidates it, and uses it as a leap off point, as well.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: hamloaf]
#25097027 - 03/28/18 11:31 AM (5 years, 9 months ago) |
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 i dont mind sediment either.
 this agar pin is covered in 'dirty' broth from the sediment. it kinda outlines the 'jellyfish'
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Tookitooki
Mycological Fabricator


Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Cultivation General Discussion [Re: van hatton]
#25097138 - 03/28/18 12:09 PM (5 years, 9 months ago) |
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Thanks ham.
I'll give the bags a shake this weekend van. These bags were just a test anyways. I sealed them after the PC, not in front of a hood or in a SAB, nor did I run sleeves. The fact they are not green, yellow, brown, slimey gooey, is a win in my book. My lc failed me tho haha. Win some loose some, all a process of learning
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Tookitooki]
#25097145 - 03/28/18 12:13 PM (5 years, 9 months ago) |
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these fuckin oats.
 heres wbs jar and an oat jar(on the right). both inoculated with the same clone LC. the wbs was inoculated a couple day after the oats. same broth..
anyway the oats finished yesterday.
  same thing with damn near all my oats. they colonize and start oozing metabolites like its sweatin the shit.
not my sexy bseed tho..
 damn i miss you gurl.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: mushboy]
#25097206 - 03/28/18 12:47 PM (5 years, 9 months ago) |
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that's how I feel about my rye.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Tight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 4 months, 8 days
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Re: Cultivation General Discussion [Re: van hatton]
#25097334 - 03/28/18 01:37 PM (5 years, 9 months ago) |
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What is the longest you guys have waited to PC grains after soaking/cooking? I cooked some last night, let them dry while I slept. Woke up this morning and loaded jars and began PC cycle. I was interrupted just as PC reached 15 psi and had to shut it off. I won't be able to do a PC run until tonight after I get off work, some 24 hours after I originally began the process.
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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aftr they were hydrated? like 12hrs
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
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Re: Cultivation General Discussion [Re: mushboy]
#25097418 - 03/28/18 02:14 PM (5 years, 9 months ago) |
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I've never waited that long. I bet you'd be ok if you simmered them quite a bit. But if they were lightly simmered, with zero burst kernels, they might be awfully dry.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 11 hours, 21 minutes
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Re: Cultivation General Discussion [Re: tedoro]
#25097558 - 03/28/18 03:29 PM (5 years, 9 months ago) |
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Ive got sone grains over 24hr past drying, but i jarred em to minimize moisture loss, speaking of which i have got to PC them or else...
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
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Re: Cultivation General Discussion [Re: Failboat]
#25097580 - 03/28/18 03:41 PM (5 years, 9 months ago) |
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I have aome fucked up agar plates where I used the shitty agar premix crap. I bought pure agar today, and I have read about the hot pour recently. Would it make sense to do a hot pour over my transfers that are on the unstable agar goop?
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Doc9151
Mycologist



Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
Last seen: 1 year, 6 months
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Re: Cultivation General Discussion [Re: Failboat]
#25098276 - 03/28/18 08:51 PM (5 years, 9 months ago) |
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Quote:
Quirkmeister92 said: I have aome fucked up agar plates where I used the shitty agar premix crap. I bought pure agar today, and I have read about the hot pour recently. Would it make sense to do a hot pour over my transfers that are on the unstable agar goop?
I would make a transfer to a clean plate.
--------------------
  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 11 hours, 21 minutes
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Re: Cultivation General Discussion [Re: Doc9151]
#25098346 - 03/28/18 09:27 PM (5 years, 9 months ago) |
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I took more transfers from the original plates. As for the transfers I previously made, the growth will be on goo basically, so I thought the hot pour would make it so I can actually cut and transfer a solid wedge.
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MurderByNumbers
Stranger

Registered: 02/13/18
Posts: 9
Last seen: 3 years, 2 months
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Re: Cultivation General Discussion [Re: Failboat]
#25098468 - 03/28/18 11:19 PM (5 years, 9 months ago) |
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Anyone know what common causes for Pan cyans aborting would be? First time, did 30 jars (lost about half from contam. using wild samples) moved these to tubs after 14 days (full colonization +5 days) Kept in the dark for 3 days then moved to 12/12
now 2 weeks later.. some look like they just recovered and did nothing beyond that, just made everything white with 0 knots forming.
some made loads of beautiful knots and began pinning (was excited) they reached between .5-1.25'' in height and have all aborted. all subsequent pins have done the same thing.
some look to be colonizing the casing layer and aren't knotting but the myc looks healthy
Humidity, temp, Gas exchange are well within acceptable ranges based on information i've found.
Main thing i can think of is that i didn't use cpoo/hpoo. Have read that it wouldn't fruit without, but i had pinning going.. not sure why it would abort though?
any help appreciated. shy to post pics :P
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