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Mr.Kaption
I breed snails


Registered: 02/16/18
Posts: 602
Last seen: 3 years, 6 months
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Re: Cultivation General Discussion [Re: Zero Nowhere]
#25094258 - 03/27/18 11:03 AM (5 years, 9 months ago) |
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Don’t mind my big shifty transfer, was my first agar plate ever. I tend to transfer early. I usually give them a little longer now.
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 1 day, 2 hours
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Re: Cultivation General Discussion [Re: Mr.Kaption]
#25094272 - 03/27/18 11:09 AM (5 years, 9 months ago) |
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OMG, I just found another contam'd plate, this time I'm almost sure its a bug! I can see the tracks in the condensation. 10 other plates are clean, just these two. Funny!
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Zero Nowhere
Beer Drinker



Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon
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Re: Cultivation General Discussion [Re: Mr.Kaption]
#25094273 - 03/27/18 11:09 AM (5 years, 9 months ago) |
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That's about what my plate looks like. Maybe not quite as advanced as yours. Maybe I'll wait a couple more days. I'm not really sure what the benefit is to transferring early or late.
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Mr.Kaption
I breed snails


Registered: 02/16/18
Posts: 602
Last seen: 3 years, 6 months
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Re: Cultivation General Discussion [Re: tedoro]
#25094276 - 03/27/18 11:10 AM (5 years, 9 months ago) |
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They are getting in wrapped plates?
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Mr.Kaption
I breed snails


Registered: 02/16/18
Posts: 602
Last seen: 3 years, 6 months
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Re: Cultivation General Discussion [Re: Mr.Kaption]
#25094287 - 03/27/18 11:13 AM (5 years, 9 months ago) |
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Early enough to out run tams but long enough to give some distance between clean myc and and possible ride along contams? That’d be my guess.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Mr.Kaption]
#25094292 - 03/27/18 11:14 AM (5 years, 9 months ago) |
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The lines in the condensation happens naturally. At least I've seen them on tons of fine plates.
I feel if it was actually mites the contamination would be in the center as well and not just the edge.
How old are those plates.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: van hatton]
#25094316 - 03/27/18 11:20 AM (5 years, 9 months ago) |
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Id bet my bottom dollar that thats from mites. I had my plants inside one year and i kept getting bad contams and every plate had those little trails on them in the condensation. On closer inspection there were tons of tiny, like damn near imperceptible to the naked eye tiny, white mites crawling around the edges of the dishes.
I almost had a mental breakdown thinking i was covered in these bitches cause there were a lot 
Havent noticed them this year. Kizzle has a good thread on them also. He was straight overrun.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: natedawgnow]
#25094321 - 03/27/18 11:20 AM (5 years, 9 months ago) |
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Trails from mites look all zigzag and thin. Water drop trails are thicker and dont stagger as much ive noticed
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 1 day, 2 hours
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Re: Cultivation General Discussion [Re: van hatton]
#25094324 - 03/27/18 11:21 AM (5 years, 9 months ago) |
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Maybe 3 days old.
Okay... its a SAB... I always accept some contams. I've just never noticed the lines before. what causes the lines in the condensation? Maybe something in the plastic petri manufacturing? The bug would get in during my transfer. but maybe its no bug. I mainly think it would be amusing if it was. I certainly can't see it.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: natedawgnow]
#25094336 - 03/27/18 11:23 AM (5 years, 9 months ago) |
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Quote:
natedawgnow said: Id bet my bottom dollar

damn, ive never seen mites like that. hope i never do. only gnats here and there during the winter months from plants being brought inside..
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: natedawgnow]
#25094341 - 03/27/18 11:24 AM (5 years, 9 months ago) |
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Quote:
natedawgnow said: Id bet my bottom dollar that thats from mites. I had my plants inside one year and i kept getting bad contams and every plate had those little trails on them in the condensation. On closer inspection there were tons of tiny, like damn near imperceptible to the naked eye tiny, white mites crawling around the edges of the dishes.
I almost had a mental breakdown thinking i was covered in these bitches cause there were a lot 
Havent noticed them this year. Kizzle has a good thread on them also. He was straight overrun.
This happened to me in a brewery lab to wrapped plates once. Either they can worm their way in or chew thru the film.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: tedoro]
#25094354 - 03/27/18 11:27 AM (5 years, 9 months ago) |
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Mites r so small they can crawl between the petri and whatever your wrapping with.
I always assumed the lines I had were just small water droplets accumulating then deciding which way to go.
I've never had plates that look like that go bad uninnoculated.
And yes kizzles thread is badass
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 1 day, 2 hours
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Re: Cultivation General Discussion [Re: van hatton]
#25094405 - 03/27/18 11:41 AM (5 years, 9 months ago) |
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I just read kizzles mite thread too.... damn, as if we don't have enough bad hombres to ward off. Mites!
I'm still unsure what mine is, I'm not concerned, but damn!!!
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Animegeek5150
Stranger


Registered: 03/19/18
Posts: 1
Last seen: 5 years, 9 months
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Re: Cultivation General Discussion [Re: mushboy]
#25094410 - 03/27/18 11:42 AM (5 years, 9 months ago) |
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So im new at growing and i wanted to know if these where knots and how long they might take https://files.shroomery.org/files/18-13/217224818-1522172188942483511715.jpg] [/url]
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: tedoro]
#25094436 - 03/27/18 11:51 AM (5 years, 9 months ago) |
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Quote:
tedoro said: I just read kizzles mite thread too.... damn, as if we don't have enough bad hombres to ward off. Mites!
I'm still unsure what mine is, I'm not concerned, but damn!!!
If you have those trails and an assortment of contams on your plate id be pretty convinced its mites. Not a rule just a thought based on my experience.
Edit: forgot to say all my plates were wrapped in plastic wrap or parafilm. They can easily make it through as well as grain filters. They can tunnel through sfd material.
I wrap my petris and put them in gallon ziplock bags now for safe measure
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Edited by natedawgnow (03/27/18 11:54 AM)
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MiracleMike




Registered: 02/15/16
Posts: 176
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Re: Cultivation General Discussion [Re: natedawgnow]
#25094457 - 03/27/18 11:59 AM (5 years, 9 months ago) |
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I wonder, why most growers photos looks like they've been shot on some chinese noname phone that was made around 10 years ago?
-------------------- Technically alcohol is a solution
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Ghostveil25
Electron


Registered: 02/04/18
Posts: 372
Loc: Oregon
Last seen: 2 years, 1 month
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Re: Cultivation General Discussion [Re: Mycolorado]
#25094466 - 03/27/18 12:03 PM (5 years, 9 months ago) |
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Quote:
Mycolorado said:
Quote:
Ghostveil25 said: This tells me that all those tubs ive recently lost to trich were caused by improper pasteurization. Im not pasturizing my substrate long enough. I think it would be fine had I not added coffee or if you do add coffee add an hour to pasteurization time. Go 3 hours instead of 2.
As mentioned you may simply have dirty spawn but your pasteurization is also likely an issue...what temps are you running? 2 hours is already too long for proper pasteurization of substrate and 3 hours will definitely ruin it. Generally, 1 hour between 150F and 160F is the target for pasteurizing most subs and casings...any longer and hotter will start to kill off the beneficial microbes, rendering the sub/casing susceptible to contams.

Generally, my temps are between 65 and 70 during colonization. At first i was pasturizing ar 150 for an hour and 15 minutes. I kept raising the time more and more after each tub would fail.
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 1 day, 2 hours
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Re: Cultivation General Discussion [Re: MiracleMike]
#25094469 - 03/27/18 12:04 PM (5 years, 9 months ago) |
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Mines def chinese. $900 iphone. shooting through crappy plastic petris.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Cultivation General Discussion [Re: tedoro]
#25094685 - 03/27/18 01:34 PM (5 years, 9 months ago) |
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Quote:
Ghostveil25 said:
Quote:
Mycolorado said:
Quote:
Ghostveil25 said: This tells me that all those tubs ive recently lost to trich were caused by improper pasteurization. Im not pasturizing my substrate long enough. I think it would be fine had I not added coffee or if you do add coffee add an hour to pasteurization time. Go 3 hours instead of 2.
As mentioned you may simply have dirty spawn but your pasteurization is also likely an issue...what temps are you running? 2 hours is already too long for proper pasteurization of substrate and 3 hours will definitely ruin it. Generally, 1 hour between 150F and 160F is the target for pasteurizing most subs and casings...any longer and hotter will start to kill off the beneficial microbes, rendering the sub/casing susceptible to contams.

Generally, my temps are between 65 and 70 during colonization. At first i was pasturizing ar 150 for an hour and 15 minutes. I kept raising the time more and more after each tub would fail.
Yeah, I meant the pasteurization temps...an hour and 15 min at 150F should be just about perfect (I pasteurize my Jiffy for casing at 150F for 1 hour). If you're properly pasteurizing, the contam is likely riding in your spawn, which is a whole other story. As mentioned, if you have access to coir, you might give it a shot as it's very simple and doesn't require any special preparation...just hydrate and add spwan. Jiffy works better as a casing than a sub.
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Ghostveil25
Electron


Registered: 02/04/18
Posts: 372
Loc: Oregon
Last seen: 2 years, 1 month
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Re: Cultivation General Discussion [Re: Mycolorado]
#25095206 - 03/27/18 04:34 PM (5 years, 9 months ago) |
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Yes, i have access to pretty much anything. This all started with a syringe and a malt extract agar. The cultures grew out and most were transfered 3 times. By the time i inoculated the wbs quart jars, i had some very clean looking disks to work with. All done in a very nice plexy glass SAB.
The jars take off and are 100% in about 8 days. I use a lot of wedges per jar. Then, i took horse manure/ coir/ coffee grounds/ verm and gypsum and sterilize in quart jars at 16psi for 2 hours. Allow to cool over night and spawn colonized jars to the substrate.
It actually starts out very nice and fluffy white. Then, usually starting around the polyfil on the sides, this dark grey mycelium shows up. It then spreads so fast that the tub is worthless within a couple of days. Within a few days these dark green valleys appear over the top and bottom. They never pin and just die. Everyone of them so far.
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