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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: AtomHeart]
#25092648 - 03/26/18 05:56 PM (5 years, 10 months ago) |
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A single fruit can be set together from more than one strain, so there is still several genetic variances with culture generated from clone.
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 1 day, 2 hours
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Re: Cultivation General Discussion [Re: Ghostveil25]
#25092701 - 03/26/18 06:17 PM (5 years, 10 months ago) |
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Hey, your story sounds like mine. Took me a while to hunt my problems down. It helped a lot to do tests to try to narrow down where the problem was. And to post photos of plates that you feel are clean.... Photos of the jars growing out that you think are clean.
I was learning bags. And my agar work was decent. But I didn't know I couldn't open a bag in a SAB. Until I tested opening one, without noc'ing it (it went bad). And when I started posting photos of grain colonizing that I thought was clean, but others highlighted bacteria I didn't know how to see.
So helpful! And I've been at it for 9 years.
T
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 13 hours, 24 seconds
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Re: Cultivation General Discussion [Re: tedoro]
#25092759 - 03/26/18 06:34 PM (5 years, 10 months ago) |
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Bags in a SAB is tricky for sure, but I think I have 2 I made work out so far. G2G is my favorite method so far.
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Zero Nowhere
Beer Drinker



Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon
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Re: Cultivation General Discussion [Re: Failboat]
#25092865 - 03/26/18 07:01 PM (5 years, 10 months ago) |
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My ms 3rd transfer culture is way more rhiz0 and growing way faster than my clone 1st transfer. Is this normal? I feel like I would rather put the ms to grain than the clone
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 14 hours
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Re: Cultivation General Discussion [Re: Zero Nowhere]
#25092881 - 03/26/18 07:08 PM (5 years, 10 months ago) |
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Whenever that happens to me I assume I've lost the genetic lottery. Could be some sort of bacteria in the culture i assume
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Zero Nowhere
Beer Drinker



Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25092903 - 03/26/18 07:14 PM (5 years, 10 months ago) |
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Quote:
tryptkaloids said: Whenever that happens to me I assume I've lost the genetic lottery. Could be some sort of bacteria in the culture i assume
You mean in the clone culture? I was wondering if it had bacteria. There was alot of weird caps in the tub it came from. I guess it looked like new shrooms were starting to grow from the caps. Is that from bacteria? Would you scrap it and go with the fast rhizo ms culture?
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 14 hours
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Re: Cultivation General Discussion [Re: Zero Nowhere]
#25092919 - 03/26/18 07:18 PM (5 years, 10 months ago) |
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My advice is worthless here. I think you had rosecomb deformities. I think they are from a type of bacteria. I would try a hot pour then transfer to a less nutritious batch.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Zero Nowhere
Beer Drinker



Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25092936 - 03/26/18 07:24 PM (5 years, 10 months ago) |
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Quote:
tryptkaloids said: My advice is worthless here. I think you had rosecomb deformities. I think they are from a type of bacteria. I would try a hot pour then transfer to a less nutritious batch.
Hot pour? I'm not sure what that is. You mean just pour some plates, instead of pre pour? Would diluting oat steep water be the same as less nutes? I'm a tard bro. I appreciate your help.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 14 hours
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Re: Cultivation General Discussion [Re: Zero Nowhere]
#25092956 - 03/26/18 07:29 PM (5 years, 10 months ago) |
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Pouring more hot agar on top of a new transfer. And yes.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Zero Nowhere
Beer Drinker



Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25092971 - 03/26/18 07:33 PM (5 years, 10 months ago) |
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Quote:
tryptkaloids said: Pouring more hot agar on top of a new transfer. And yes.
Gotcha, thanks bud. I'll give it a try.
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MiracleMike




Registered: 02/15/16
Posts: 176
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Re: Cultivation General Discussion [Re: Zero Nowhere]
#25093456 - 03/27/18 12:08 AM (5 years, 10 months ago) |
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OMG IT'S PINNING!

Fuck yeah I'll triping this Fool's day!
-------------------- Technically alcohol is a solution
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Ghostveil25
Electron


Registered: 02/04/18
Posts: 372
Loc: Oregon
Last seen: 2 years, 1 month
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Re: Cultivation General Discussion [Re: MiracleMike]
#25093546 - 03/27/18 01:35 AM (5 years, 10 months ago) |
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Just wanted to say that im almost positive that ive found my source of contamination. Ive mixed spawn quarts with equal parts of jiffy seed starter plus a jiffy casing of about 1/2 inch. This is taking off like you wouldnt believe.
This tells me that all those tubs ive recently lost to trich were caused by improper pasteurization. Im not pasturizing my substrate long enough. I think it would be fine had I not added coffee or if you do add coffee add an hour to pasteurization time. Go 3 hours instead of 2.
I eliminated all nutritive sources down to just my spawn and jiffy mix and there is no contamination.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Ghostveil25]
#25093993 - 03/27/18 08:50 AM (5 years, 9 months ago) |
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jiffy mix isnt for bulk. its for casing. which needs proper pasteurization because it has peat in it.
why not use coco coir and skip the coffee? it doesnt need past. problem solved unless your spawn is still dirty
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MiracleMike




Registered: 02/15/16
Posts: 176
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Re: Cultivation General Discussion [Re: mushboy]
#25094085 - 03/27/18 09:38 AM (5 years, 9 months ago) |
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Agre with mushboy. I use coco coir with current grow as substrate and as a casing. If aply it in right moment it won't be colonized. Many people says that cubes need no casing, but I find it very usefull in my grow. And yes, it is very easy. Easy AF. Just grains and just coir. In first grow I used peat moss and verm 50/50 for BRF cakes and got trich in the middle of second flush. I can't say this is an issue, it's just were soooo difficult. I had to make BR flower in blender, I had chop peat moss.. This lots of action stops me from growing second time for two years. Then recently I've lurked shroomery for easier TEKs and found BODs EASY AF TEKs. Then I was like "Jiziz! Fuck this cake shit, I'm doin grains and coir!"
-------------------- Technically alcohol is a solution
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 1 day, 2 hours
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Re: Cultivation General Discussion [Re: MiracleMike] 1
#25094111 - 03/27/18 09:52 AM (5 years, 9 months ago) |
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Mike~ When you add your coir casing at "just the right time" to keep it from colonizing, when might that be?
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Cultivation General Discussion [Re: Ghostveil25]
#25094132 - 03/27/18 10:06 AM (5 years, 9 months ago) |
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Quote:
Ghostveil25 said: This tells me that all those tubs ive recently lost to trich were caused by improper pasteurization. Im not pasturizing my substrate long enough. I think it would be fine had I not added coffee or if you do add coffee add an hour to pasteurization time. Go 3 hours instead of 2.
As mentioned you may simply have dirty spawn but your pasteurization is also likely an issue...what temps are you running? 2 hours is already too long for proper pasteurization of substrate and 3 hours will definitely ruin it. Generally, 1 hour between 150F and 160F is the target for pasteurizing most subs and casings...any longer and hotter will start to kill off the beneficial microbes, rendering the sub/casing susceptible to contams.
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 1 day, 2 hours
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Re: Cultivation General Discussion [Re: Mycolorado]
#25094158 - 03/27/18 10:21 AM (5 years, 9 months ago) |
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I thought this petri contam was weird. I haven't been getting many lately. Then I wondered if a tiny fly got in, when the lid was off. (I'm using a SAB) The contam is in long little lines, perhaps footprints?
I guess more likely is a water drop rolling around. I can't remember now.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
Edited by tedoro (03/27/18 10:23 AM)
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MiracleMike




Registered: 02/15/16
Posts: 176
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Re: Cultivation General Discussion [Re: tedoro]
#25094170 - 03/27/18 10:30 AM (5 years, 9 months ago) |
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I can't express this kind of knoledge in numbers or some kind of "omens". This is mostly intuitive. I waiting until tray is really near pinning. I don't know for sure, but I think I read something about this, like Late Casing TEK. The point is to apply casing as close as possible to pinning process, or right when you see the first bébés. Mycelium is kinda switching from expansion (growing) mode to fruiting and not interested much in your yummy casing.
On the first photo you can see my coir casing slightly colonized but it is also a thin one. And pins are coming though. I case that tray 4 days before pinning. It was the 9th day after spawning grains to coir and the 5th day after putting tray to FC. On the second photo you can see tha same tray cased during spawning and look how this double time thicker casing layer was colonized in 3 days.
-------------------- Technically alcohol is a solution
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 1 day, 2 hours
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Re: Cultivation General Discussion [Re: MiracleMike]
#25094189 - 03/27/18 10:36 AM (5 years, 9 months ago) |
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Cool. I've always enjoyed having some uncolonized coir on the surface. I feel less cautious about adding water if there is that buffer.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Zero Nowhere
Beer Drinker



Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon
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Re: Cultivation General Discussion [Re: tedoro]
#25094192 - 03/27/18 10:38 AM (5 years, 9 months ago) |
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How soon do you guys transfer away from germination plate? As soon as you see myc?
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